Could Wolfram Alpha facilitate life extension and anti-aging? Specifically how?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important:

1. Open ssDNA bubble within replicable (in E. coli) genetic element. So no C-Traps under force.

2. No proteins, nucleic acids, or other toxic chemicals supporting the bubble. Can help during nucleation, but bubble has to be accessible for protein interaction.

3. Stable in bioorthogonal conditions. Physiological pH, salt, 37 °C, etc.

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

Do 2 plasmids containing one lac and one tac promoters induce protein expression in the same cell?

I have to estimate the growth of my bacterial cultures spectrophotometrically and I read articles of measurement at an OD of 600nm. Also what to do if the values exceed 1. What is the proper method for the measurement of the same.

In the identified case of familial desminopathy (T341P DES mutation in heterozygous state), the son has bradycardia, but the father did not have bradycardia. How can this fact be explained?

In my study, I observed a significant difference between the mean expected heterozygosity (He) of 0.88 and the observed heterozygosity (Ho) of 0.40. I am seeking insights into the potential causes for this discrepancy. One possible explanation could be inbreeding, which increases homozygosity and lowers observed heterozygosity. Another factor might be genetic drift, particularly in small populations, where random changes in allele frequencies can lead to a reduction in heterozygosity. I would appreciate any further explanations or insights into these or other possible reasons for such a substantial difference between He and Ho in my study.