Asked 14th Jan, 2013
Could you tell me how I can transform raw information of the brainwaves in Hetz?
I'm using Neurosky technology, but the information about brainwaves are in raw. Then my goal is to transform raw information in Hertz, like the brainwaves theory says.
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How to analyze cross-correlation data ?
- Livio Oboti
Can anyone suggest an efficient and standard method to analyze differences between cross-correlograms related to a group of pair recordings (each pair has a cross-correlogram) in different conditions ?
My aim is to assess statistical significance among cross-correlation values in 4 different conditions (each pair has four recording conditions).
The software MiniAnalysis was used to calculate the cross-correlogram for each cell pair but it does not contain any routine to run this statistical analysis (I might double check but it seems to me it doesn't).
I have been suggested to use Bootstrap analysis or trial shuffling. I found so far only theoretical articles about both methods, none streamlining the procedure from a more practical standpoint. In addition, although I have understood the general meaning of these tests, I was wondering whether there are caveats or exceptions I should take into account, given that my "measures" are "repeated" for each element of the sample (each element/subject/pair has 4 measures that need to be compared).
Last: I do have access to Matlab but I am not a matlab user, so any suggested method that would avoid the use of this software would be preferred.
What neuron excitability properties are most important for publication?
- Cody Boyle
I read papers that report several neuronal properties between experimental and control conditions and wonder how I should be selecting data to report on. I see some trends. Spike half-width, rheobase, input resistance, capacitance, and RMP are often shown. Some include others.
Why? How do I choose which to share. Do people report statistically significant findings?
I am wondering what data to report and why? Are these data points often include to please manuscript reviewers?
Can anyone point me to any literature to explain what neuronal properties are most important to show?
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Does profession have the larger influence on mindset and decision making than culture?
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I remember reading a publication or commentary about this once, but I do not at all recall the source. Is there anyone that recalls said document?
Why is the impedance of ECoG electrodes tested at a frequency range from 1 Hz -100 kHz?
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I am testing the impedance of a material that will be used for ECoG electrodes.
I was told to test it at a frequency range from 1 Hz - 100 kHz but why is this range used? I wasnt able to find a satisfactory answer.
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MEA Neuronal Activity Assay?
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How to depolarize cultured primary neurons with KCl without them dying?
- Max Karasev
Dear colleagues, I’ve started working with cultured primary neurons and came across a problem.
I need to depolarize neurons for different time intervals (up to 1 hour) and then use them for an assay 2 days after. The problem is that most of them are dying after depolarization.
I culture neurons in complete neurobasal medium (Neurobasal + 2% B27 supplement + 1%Glutamax +1% penstrep) with 1/3 media change every 3 days. I depolarize them on DIV11-14 by swapping media on Tyrode’s solution (45mM KCl) for up to an hour. Then I wash the cells with Tyrode’s solution (5mM KCl) twice and swap the collected media back.
45mM K+ Tyrode’s: 100 mM* NaCl; 45 mM KCl; 1 mM MgCl2; 1.8 mM CaCl2; 1.04 mM Na2HPO4; 26.2 mM NaHCO3; 10.9 mM HEPES; 10 mM D-glucose
* NaCl is used to adjust osmolarity of the solution, so concentration varies.
Since in the current setup I depolarize cells in 5%CO2 incubator I used buffering formula of Neurobasal media. I adjust the pH to Neurobasal’s pH=7.7 and checked that in CO2 incubator it equilibrates to pH=7.4. And I adjust solution’s osmolarity to match the current neuron’s medium too.
Also, I depolarized neurons in live cell imaging using GCaMP6s to monitor calcium elevation and after minutes I can see that some neurites are destroyed (Fig.1, attached) and after 0.5-1 hour cells don’t look good and most of them die afterwards (Fig.2).
At the same time, I keep seeing papers with no explicit details on solution and osmolarity where cultured primary neurons are stimulated with KCl for hours. For example, here 6 hours of 55mM KCl (https://www.nature.com/articles/nature09033).
I guess there are a lot of people routinely working with primary neuronal culture. Could you please help me, what am I missing?
Dweck's theory: Flores adaptation - cut off points to categorize parents as Growth Mindset-parents and Fixed-Mindset parents?
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Can you please help me with the Dweck's theory of implicit intelligence? I found an 8-item scale by Flores but there are not any cut off points in order to categorize the parents I intend to measure as Fixed mindset parents or Growth Mindset parents.
According to Dweck's theory I want to investigate parental characteristics and controlling for their mindset score. In the articles I find no cut off points are claimed.
In a mindset quiz (no writer available) the suggested cot-off points are:
Strong growth mindset: 45-60 points,
Growth mindset with some fixed ideas: .34-44 points,
Fixed mindset with some growth ideas: 21-33 points and
Strong Fixed mindset: 0-20 points.
The intervals are not equal.
Is there any justification for this?