Constructivism(with privilege heritability) and intelligent design both impact the evolutionism creationism debate.

What is the relationship between the maximal density of a bacterial population during anaerobic and aerobic growth for a specific species - a facultative anaerobe?

I'm working with a microbial consortium in a bioreactor. The microbial community acts as a black box, and I'm trying to elucidate what's inside and how it changes over time. I'm planning to perform metagenomic analysis and MAG reconstruction at time point 1 and then observe what happens at later time points.

I'm planning to take samples at more than two time points. I'm a bit unsure whether I can reconstruct MAGs just once—using data from the first time point—and then use those MAGs to align the reads from the other time points, or if I should reconstruct MAGs separately or jointly using reads from multiple time points.

I'm planning to see how the presence/absence and abundance of the microorganisms in the consortia change over time in the bioreactor system. I would appreciate any paper/review recommendation to read.

Greetings, everyone.

I'm currently optimising a protocol on eDNA extraction from freshwater samples for a species-specific assay. I want to test multiple workflows, including either filtration or centrifugation of water samples. (Both approaches have been previously shown to be reliable for the species of interest.) Here's the thing: for centrifugation, I plan to use at least 200 ml of water split into 50 ml falcon tubes. After centrifugation, the eDNA-containing pellets should be pooled into a single 1.5 ml microcentrifuge tube for DNA isolation. The thing is: how would you go about the pellet-pooling part? I've already tested it, and the best thing I've figured out is to carefully remove as much supernatant as possible and then gently aspirate the pellet into a large pipette tip. However, it feels somewhat unwieldy and I'm afraid this step may introduce unnecessary inconsistencies. If you have experience with this sort of pellet transfer and pooling, do you have any tips on how to get the best results possible?

(The already-published papers only mention that pellets were pooled, without additional specification. I plan to contact their authors to ask for details, but having some do's and don'ts from a larger number of people cannot hurt.)

Easy, Reliable and cost effective laboratory tests available in India

Hi all,

I recently sequenced RNA from E. coli, and the FastQC report shows multiple warning symbols. Specifically, I want to know if a duplication level exceeding 70% would still make the data suitable for further analysis.

Any suggestions are welcome!

Anuradha

Is anyone using the differential supercoiling anatomy of DNA for phylogenetics: Any ideas on Phylogenetic Signal in such Data?