Question

Asked 25th Feb, 2019

Defence Research and Development Organisation

Can anyone suggest the complete Experimental Process with possible protocols ?

I am working to find a bioactive compound from a bacterial source which can show antifungal activity. So I have established the bacterial culture in a synthetic medium. What we found that the enzyme/proteinous compound is mainly extracellular in nature. Now I want to go for isolation, extraction and purification of the compound from the liquid culture of bacteria at laboratory scale. So I am asking to share your knowledge about the sort of suitable methods which will be helpful for getting the desired compound.

Bacterial Cell Culture

Bioactive Compounds

Antifungal

Purification

Bioprocess Engineering and Fermentation Technology

Bioprocess Engineering

Extraction

Enzyme Purification

Enzyme Biotechnology

Enzyme Activity Assay

Protein Extraction

Extracellular Enzymes

Antifungal Activity

24th May, 2020

Amir Reza Abbasian

Shahed University

Try this website protocols

http;//www.BioDataCenter.ir

Cite

Laith Al-Ani

University of Baghdad

Alaa Hani Al-Charrakh

University of Babylon

Dhifaf Zeki

University Of Kufa

Rana Kadhim Mohammed

University of Baghdad

Nuha Zubbair

Kwara State Polytechnic, Ilorin, Nigeria

25th Feb, 2019

Manuele Martinelli

checkmab srl

Dear Swaraj

there is no a simple and Universal answer to your question, it depends from the properties of the bioactive compounds (eg it is a protein, a small molecule, which is mw? pi? ) and of the media from which it need to be recovered and purified.

ciao

Manuele

Cite

25th Feb, 2019

Swaraj Mohanty

Defence Research and Development Organisation

Manuele Martinelli

Sir,

Thank you for your wise suggestion. Our interest is limited to the proteinous bioactive compounds(as we believe that most enzymes are proteinous in nature). And next is the bioactive compounds are mainly extracellular in nature(We have checked that by performing experiments). And once we extract it and then only we can find the MW & PI of the protein along with the estimation of the proteinous compound. We have the bacterial liquid culture(maintained in a synthetic media similar to LB media with minor modification).

So I want to know the general isolation & extraction procedure for gram positive bacteria(our bacteria belongs to Bacillus sp. ).

Cite

25th Feb, 2019

Yogesh Kumar Negi

College of Forestry, VCSG UUHF, Ranichauri

Dear Swarj you can isolate the desired compound by salting out method or ion exchange chromatography.

Cite

24th May, 2020

Amir Reza Abbasian

Shahed University

Try this website protocols

http;//www.BioDataCenter.ir

Cite

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