Can I use basta as a selectable marker in fungal tranformation?

Sigma lysing enzyme L1412 has been discontinued. Is there a known commercially available alternative to this ?

For those in the area of either archaeogenetics or forensic genetics, are there any firm opinions on which human body parts function to preserve DNA best during decomposition, and would therefore be the the most suitable parts to process when extracting DNA for sequencing?

Does the answer change depending on whether the remains are somewhat recently buried (past few years) versus decades to hundreds to thousands of years old? Does the answer also depend on whether the body underwent preservation techniques, like embalming? I'm asking for situations where the body has not been frozen.

I have read that some specific types of teeth and bones may be ideal candidates, but any additional insight, experience, or nuance would be greatly appreciated.

I’m looking for a rapid method to extract protein from tobacco plant tissue, using up to 1-2 grams of leaves, with sufficient yield for protein analysis such as Western blot. I need to screen hundreds of plants, so the method should be as simple and efficient as possible.

Thank you for your advice.

I found that fingerprint analysis of oligonucleotide fragments was used in molecular biology in some top-tier journals in the 1970s, but it seems difficult to find such experimental methods in current journals. Why is that? Does this experimental approach have any defects? What methods can be used to replace it now?

a small protein (~5KDa) is tagged with HA chromosomally. On doing western blotting and probing blot with anti HA antibody, the protein is showing band at ~13 kDa whereas expected size is ~6 kDa. Resolving gel used is 15%. How to resolve this problem?

I am planning to order an antibody, but for the commercially available antibody, it is written that the application is ELISA. So, if I want to order the same antibody can we use that?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan while I am sure about the dilution and the similar RNA load since I confirmed the CT values by the qPCR and they were the same nearly ( Photo attached)

Any suggestions?

The procedures involve

1- Extraction of Total RNA by the Invitrogen kit

2- Dilute the RNA so that 200ng/ul is there

3- Denatutartin the RNA 95oC for 5 min then on ice till loading

4- load the RNA 5ul on the membrane ( total 1 ug/spot)

5- Air dy 15 mins

6- UV cross-linking 2 mins twice

7-Wash by PBST 5 mins

8- Block with 5% skimmed milk in PBST

9- Primary antibody 1:1000 in 5% skimmed milk in PBST over nigth in 4 C

10- Wash three times PBST 5min each

11- Secondray antibody 1:3000 n 5% skimmed milk in PBST, 2 hours in the room temperature

12- Wash three times PBST 5min each then imaging