literature shows glucose is probably the best source of carbon for bacillus species.
I'm now growing it in Nutrient broth at 37C, and I've found no problem.
is there any selective media
LB is not the best of choices. I don't know what you intend to do, but normally you will like to have synchroneously developing cultures that go deliberately into spore Differentiation.
The best medium for Bacillus subtilis, where the cells grow rapidly during the exponential phase, normally (with the 168 derivatives, but not with all strains) show a clear T-zero at the beginning of the stationary phase and sporulate pretty synchroneously with normally more than 80 % efficiency is NSM or - if you want to have it buffered (which I normally preferred) NSMP.
For good sporulation, it is never a good idea to add glucose as carbon source, due to catabolite repression.
Start with NB-Medium: 8 g Nutrient Broth (Difco) per l, autoclaved.
Add per 100 ml NB: 1. 0.5 ml Metal Mix (14 ml 1 M Ca-chloride; 10 ml 0.1 M Mn chloride; 20 ml 1 M Mg chloride; 56 ml water), sterile-filtered or made from pre-sterilized stock solutions 2. 0.05 ml Fe-solution (32.4 mg Fe(III)-chloride; 1 ml 1 M HCl in 100 ml water), sterile-filtered 3. 5 ml Phosphate buffer (95 g K2HPO4 x 3 H2O, 124 g KH2PO4, ad 1l water), autoclaved
and that's it. It works.
Institute for Genetic Engineering
Friedrich Schiller University Jena
Provinciale Hogeschool Limburg
University of Science and Technology of China
Malihe vahidi nasab
University of Tehran
National University of General San Martín
University of Agriculture Faisalabad
National Institute of Technology Rourkela
An error occurred while rendering template.