BCM7 from AI protein in milk?

Hello,

I am very new to the FTIR technique and recently stumble upon an observation that I cannot explain.

So when I analyzed the native proteins, the spectra showed no transmittance in the Amide A range. But when the protein was hydrolyzed first prior to measurement, it showed a peak in Amide A range.

From reading several books and publications, I understand that Amide A corresponds to the bond between N and H in peptides. So why does it require hydrolysis of a protein into peptides in order for this peak to show?

I am developing an MRM method for multi-analyte quantification using triple quad mass-spectrometer. Shall I go ahead with single transition which is stable and most abundant for each analyte or should I select at least two transitions for each analyte in my experiment. Which will be the better way. I will be great to learn about this from you all . Thanks and Regards, Vaibhav

I ordered a synthetic peptide (sequence = MHHHHHH)

This peptide has a molecular weight of 0.97 kDa based on a web-based prediction tool and mass spectrometry.

However, when I ran it on the SDS-PAGE, I can see the protein bands around 10~15 kDA

What can be potential problems?

The picture shows the result of my 16.5% SDS-PAGE.

I ran the SDS-PAGE a little bit because the peptide was too small and I thought it might escape from the SDS-PAGE

I have 5 sets with 200 variables.

Hi guys, I am purifying a protein by Ni-NTA column, but I am getting two bands in SDS-PAGE. What do you know to fix this problem?

Please.

Hello everyone!

I am facing a problem that PDF does not build properly during the submission of a revised manuscript in a journal. I contact the Team of that journal, they replied me but i did not understand. Kindly if anyone can understand or solve this problem. I will be grateful for him. Following are the steps:

Please be so kind to go through the following steps below, then rebuild the PDF.

1. Please verify that all associated TeX related files ( .sty, etc) files are uploaded to the submission.

2. Please verify that files are not referenced in sub-directories/subfolders, otherwise the paper will not build properly.

3. Please verify that all the .EPS image files are uploaded to the submission.  Please verify that the EPS image files are not referenced in sub-directories/subfolders, otherwise your paper will not build correctly.

4. Please verify the EPS image name is correct in your TeX file.

After steps 1-4 above are reviewed/corrected, please rebuild your submission.

For example: I recently downloaded a nice freeware (SeeMS) to visualize spectral data from Thermo's .RAW files.

However, I cannot work with data processing from home, as only PCs at the lab are licensed to use XCalibur, and no other freeware seem to extract data from these files in the same way I do with XCalibur. The most basic processing I am used to do is generating a mass vs. intensity table to work with Excel and multivariate data analyses software.

Can anyone help me with a freeware with these resources?

Thanks in advance!

What should be the column height and diameter ratio in protein size exclusion chromatography?

Hi, I found some new metabolites of a compound through urine sample analysis and I wanted to quantify those metabolites using UPLC-MS/MS. The metabolites standard is not available. Therefore, without knowing the Q3 how can I quantify those metabolites?

Though, I've the MS/MS fragments of those metabolites (acquired during metabolite identification study using UHPLC-Orbitrap-MS). Please share your valuable experiences and suggestions.

Thank you.

Regards,

Md. Mamunur Rashid.