About the lab

The laboratory Aquatic Animal Housing is located at Federal University of Lavras in Minas Gerais state, Brasil. The laboratory conducts pioneering research using the zebrafish model to study ecotoxicology, genetics of alcoholism, obesity and farmacology.

Featured research (1)

Due to its multifactorial and yet to be fully understood origin, ethanol addiction is a field that still requires studies for the elucidation of novel genes and pathways that potentially influence the establishment and maintenance of addiction-like phenotypes. In this context, the present study aimed to evaluate the role of the LRRK2 pathway in the modulation of ethanol preference behavior in Zebrafish (Danio rerio). Using the behavioral Conditioned Place Preference (CPP) paradigm, we accessed the preference of animals for ethanol. Next, we evaluated the transcriptional regulation of the gene lrrk2 and the receptors drd1, drd2, grin1a, gria2a, and gabbr1b in the zebrafish brain. Additionally, we used a selective inhibitor of Lrrk2 (GNE-0877) to assess the role of this gene in the preference behavior. Our results revealed four distinct ethanol preference phenotypes (Light, Heavy, Negative Reinforcement, and Inflexible), each showing different transcriptional regulation patterns of the drd1, drd2, grin1a, gria2a, and gabbr1b receptors. We showed that the lrrk2 gene was hyperregulated only in the brains of the animals with the Inflexible phenotype. Most importantly, we showed, for the first time in the context of preference for ethanol, that treatment with the GNE-0877 inhibitor modulates the transcription of the target receptor genes and reduces the preference for ethanol in the animals of the Inflexible group. This result corroborates the hypothesis that the LRRK2 pathway is involved in the inflexible preference for ethanol behavior. Lastly, we identified a possible pharmacological target for the treatment of abusive preference behavior for ethanol.

Lab head

Luis Solis-Murgas
Department
  • Departamento de Medicina Veterinária
About Luis Solis-Murgas
  • I am a professor and researcher at Universidade Federal de Lavras in Brasil. My main research interests lie in the areas of Fish Physiology, ecotoxicology and animal reproduction. https://orcid.org/0000-0002-0066-7505

Members (8)

Daniella Paula
  • Universidade Federal de Lavras (UFLA)
Isadora M. Paiva
  • Federal University of Minas Gerais
Tassia Flávia Dias Castro
  • Universidade Federal de Lavras (UFLA)
Isabela Martins Di Chiacchio
  • Universidade Federal de Lavras (UFLA)
Moises Silvestre de Azevedo Martins
  • Universidade Federal de Lavras (UFLA)
Gilmara Junqueira Machado
  • Universidade Federal de Lavras (UFLA)
Fernando Marcos Rubim
  • Universidade Federal de Lavras (UFLA)
Isadora Marques Paiva
Isadora Marques Paiva
  • Not confirmed yet
Bárbara do Carmo Rodrigues Virote
Bárbara do Carmo Rodrigues Virote
  • Not confirmed yet
Aline Ferreira Souza de Carvalho
Aline Ferreira Souza de Carvalho
  • Not confirmed yet