Lab

Experimental Thoracic Surgery Lab

About the lab

The lung epithelium represents an interface between the body and its environment. Many pulmonary diseases like viral infection, bacterial pneumonia and lung cancer formation start here. Our lab strives to clarify the underlying pathomechanistic processes to contribute to the development of new treatment approaches in lung diseases. Being a surgical lab we keep an eye on translation of research findings into the clinical setting. Our group has far-reaching experience in tissue engineering and 3D airway tissue models generated from primary human cells. These human tissue models help us and our scientific partners to close the gap between basic research and clinical application.

Featured projects (4)

Project
Clinical study on the surgical treatment of primary pneumothorax.
Project
Applying tissue engineering technologies to generate a human lung cancer model for applied research.
Project
Development of a lung perfusion device that restores ventilation and perfusion of human and porcine lungs. The device represents a technology platform for translational research approaches in pulmonary medicine.
Project
Generation of three-diemnsional (3D) airway models by tissue engineering techniques for infection research.

Featured research (9)

Introduction: The human breath is an accurate but complex read-out of many physiological processes in the organism that can be monitored via volatile organic compounds (VOCs) in the exhaled air. However, there are many confounding variables that limit the transfer and application of breath analysis to become a clinical procedure. Method: This work aims to establish a systematic procedure for sampling and characterization of various everyday influences of healthy subjects using proton transfer reaction-mass spectrometry (PTR-MS). In order to limit the influencing factors on the breath profile, a standard analysis procedure for sampling and evaluation of the exhaled breath samples was developed. The correlations between the selected experimental conditions and the resulting VOC profiles were investigated using a non-parametric Wilcoxon rank sum test. Results: In addition to the relevant influence of methodological experimental parameters, interesting insights into the effect of everyday factors on the exhalat gas were obtained and discussed. Furthermore, subject and condition-specific differences were found in the exhaled air of male and female subjects. Conclusion: With a more robust, standardized and reproducible breath sampling protocol, breath analysis is a promising non-invasive tool towards a system-wide understanding and personalized diagnosis and treatment of a wide range of diseases.
In vitro aufgebaute humane Atemwegsmodelle können die menschliche Physiologie von Atemwegsepithelien besser nachbilden als Modelle mit Zelllinien. Zelllinien weisen im Vergleich zu primären Zellen Limitationen in der Zellfunktion auf, wie z.B. das Fehlen von Tight Junctions. Die Verwendung von primären Zellen ist jedoch anspruchsvoller, da diese nicht beliebig oft passagiert werden können. In 3D-Modellen verbessert eine Co-Kultivierung mit Fibroblasten auf einer biologischen Matrix und der apikale Kontakt zu Luft (Airlift) die Differenzierungsfähigkeit.
Penetrierende Thoraxtraumata stellen eine Verletzung mit signifikanter Morbidität und Mortalität dar. Abhängig von dem klinischen Zustand des Patienten und den erwarteten Verletzungen stehen hierbei verschiedene Zugangswege zur Verfügung. Vor dem Hintergrund eines möglichst niedrigen zusätzlichen Operationstraumas („second hit“) sind der Nutzen und die Limitationen von minimalinvasiven Zugangswegen gegenüber konventionellen offenen Zugängen abzuwägen.

Lab head

Thorsten Walles
Department
  • Clinic for Cardiac and Thoracic Surgery
About Thorsten Walles
  • Thorsten Walles is Full Professor for Thoracic Surgery and Chief of the Thoracic Surgery service at the Magdeburg University Hospital (Germany). His clinical interests are thoraco-oncologic surgery as well as robotic thoracic surgery. Scientifically, he develops and applies tissue engineering approaches for basic and applied research in thoracic and pulmonary medicine.

Members (5)

Henning Busk
  • Otto-von-Guericke-Universität Magdeburg
Helena Linge
  • University Hospital Magdeburg
Julian Maurer
  • Otto-von-Guericke-Universität Magdeburg
Hailong Wang
  • Otto-von-Guericke-Universität Magdeburg
Ajay Wagh
  • Otto-von-Guericke-Universität Magdeburg
Marie-Louise Reckzeh
Marie-Louise Reckzeh
  • Not confirmed yet
Bastian Fakundiny
Bastian Fakundiny
  • Not confirmed yet
Isabell Knoblich
Isabell Knoblich
  • Not confirmed yet
Julian Maurer
Julian Maurer
  • Not confirmed yet
Carla Bever
Carla Bever
  • Not confirmed yet

Alumni (5)

Kathrin Kehrer
  • University Hospital Würzburg
Lisa Mayer
  • University of Wuerzburg
Private Profile
Private Profile