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Featured research (6)
The current study was conducted in the postgraduate laboratory of the college of Agriculture/University of Al-Muthanna/Department of Animal Production, which aimed to find out the effect of follicle diameter and the culture medium on the percentage of in vitro maturation of sheep oocytes (Ovis Aries). The experiment included in vitro maturation of sheep oocytes aspirated from large, medium and small follicles in different culture media (named A, B and C). In vitro maturation of oocytes was carried out using three culture media that differed only in the concentration of sucrose, which were 0. M, 0.25 M and 0.5 M for A, B and C culture media respectively. The results of the current study showed significant superiority (P ≤ 0.05) of culture medium C than the two media A and B in the percentages of in vitro maturation, which were to 38.0 ± 1.71 %, 27.37±1.47 % and 21.902±0.76 % for C, A and B respectively. The results also indicated significant effect (P ≤ 0.05) of follicle diameter in the percentages of in vitro maturation of sheep oocytes which were 38.0±1.71 %, 29.57±2.06 % and 18.5± 0.27 % for large, medium and small follicles respectively. It could be conclusion from the present study that the follicle diameter and culture medium had a significant effect on the percentage of in vitro maturation of sheep oocytes.
The experiment was conducted in the Agricultural research's station due to the College of Agriculture/ Al-Muthanna University in the south of Iraq, to study the effect of adding soybean milk and omega3 to the tris extender on the quality of Dama.Dama (D.Dama) Gazelle semen under cryopreservation-thawing conditions. The semen was collected from four adults males of D.Dama Gazelle using electric ejaculator system .The experiment was contains four treatments :the control treatment using the traditional tris extender only , T1.adding 5% soybeans to the tris extender , T2.adding 2% omega3 to the tris extender, T3. Adding 5% soybeans + 2% omega3 to the tris extender. It was observed highly significant effect(p≤ 0.01) of adding 5% soybean(T1) and adding 5% soybean+ 2% omega3(T3) to the tris extender on the sperm total motility% and progressive motility % associated with highest viability percentage(explained by the percentage of live sperms) and significant superiority in the results of the HOST test was noticed in this two treatments (T1 and T3).This results refers to better cryopreservation success of D. dama Gazelle spermatozoa in particular it was associated with a very good results of acrosome integrity in all treatments, this is the first study in Iraq deals with cryopreservation of D.Dama Gazelle semen, that helps to application of artificial insemination and (for the future) to conservation of the genetic resource for this endangered species.
This experiments was conducted in the laboratory of High Institute of Infertility Diagnosis and Assisted Reproductive Technologies in Bagdad, to investigate the effects of ultra rapid cryopreservation (vitrification) on the normal morphology and viability of ewes early stage embryos. The sheep ovaries were collected from the slaughters ewes immediately after slaughtering ,the immature oocytes were subjected into in vitro maturation(IVM) and in vitro fertilization(IVF) programs .The early embryonic stages were cryopreserved using the vitrification technique, then thawed and assessed from the morphology and viability at two periods : post thawing and 2hr.post thawing. The morphology and viability of early embryonic stages, showed no significant differences in two pronuclear (2PN) at post thawing and 2hr.post thawing periods namely 88.88 and 83.33% respectively. While the 1-2cell showed a higher percentage of normal morphology (93.33%) at the post thawing and 2hr.post thawing periods,also showed a high percentage of normal morphology 3-4cell embryos at post-thawing and 2hr.post-thawing (86.66%). A high percentage of viable 3-4 cells embryos were obtained at post-thawing (93.33%),which were decreased to 86.67% at 2hr.post-thawing .These results described for the first time in Iraq, using the vitrification technique with 2PN,1-2cells,3-4cells embryonic stages. This will participates in improving the reproductive efficiency of sheep via producing large numbers of embryos which could be used for application of reproductive technologies .
This experiment was conducted at the station of sheep and goat research's / the state board of agricultural research ,the artificial Insemination Department belongs to the Ministry of Agriculture and in the High Institute of Infertility Diagnosis and Assisted Reproductive Technology at Al-Nahrain university, Baghdad, Iraq to investigate the effects of cryopreservation on some ram semen characteristics. Six Turkish Awassi rams were used for semen collection and evaluation. several samples of ram semen were collected and evaluated for Volume, pH,color,Sperm concentration ,Mass activity and individual motility using computer assisted semen analysis (CASA) Dead and abnormal sperm and DNA fragmentation index (DFI). Then the semen was diluted 1:19(semen: diluents v/ v) using a Tris diluents and subjected into slow cryopreservation program .The semen samples were assessed after freezing-thawing. Cryopreservation causes a clear decadency in some parameters of ram semen .Result shows that there was a significantly (p< 0.01) affect of cryopreservation on the dead sperms percentage (31.6 ± 1.92), individual motility(IM)(31.33± 1.20%) and DNA fragmentation index (DFI) (29.83± 4.45%). Introduction :