Toxics

Exposure to fine particulate matter (PM2.5) is linked to increased systemic inflammation, particularly in individuals with metabolic syndrome (MS). This study assessed the impact of nutrition and PM2.5 exposure on inflammatory markers in individuals with MS. A total of 50 participants (25 with MS, 25 healthy controls) were monitored during a high-PM2.5 exposure period (HEP) and a low-PM2.5 exposure period (LEP). Dietary intake, health assessments, and inflammatory markers—TNF-α, IL-6, and CRP—were evaluated. The MS group had significantly higher BMI, fasting blood glucose, and triglyceride levels and lower HDL-C than the healthy group (p < 0.01), but these parameters did not change significantly between the HEP and LEP. Notably, dietary fiber intake increased in the MS group during the LEP (p < 0.05). CRP levels were higher in the MS group and significantly decreased in both groups during the LEP (p < 0.05). IL-6 was higher in the MS group during the HEP but did not significantly change across periods. TNF-α showed no differences. Dietary fiber intake was inversely correlated with IL-6 and CRP in the healthy group and strongly correlated with CRP in the MS group (r = −0.403, p < 0.01). Antioxidant vitamins were inversely correlated with inflammation only in healthy participants. These findings suggest that an increased dietary fiber intake may help reduce PM2.5-induced inflammation, particularly in individuals with MS.

Chromium (Cr) contamination poses severe risks to plant health and soil quality, requiring sustainable remediation methods. This study explored the synthesis of zirconia nanoparticles (PF-ZrO2 NPs) from Sonchus asper extract and assessed their potential to alleviate Cr toxicity in Chinese cabbage (Brassica rapa var. pekinensis). The characterization of nanoparticles was performed through XRD, SEM, and FTIR analyses, confirming their crystalline nature, structure, and surface chemistry. The results indicated significant declines in plant growth, chlorophyll content, biomass, and nutrient uptake under Cr stress (treatments T2 and T4), accompanied by elevated oxidative stress indicators (H2O2, MDA) and Cr accumulation. The application of PF-ZrO2 NPs (T3 and T5) notably reduced shoot Cr concentrations (by 58.94% and 35.90%) and improved the chlorophyll level (by 5.41% and 14.41%). Additionally, nanoparticles increased antioxidant enzyme activity (SOD, POD, CAT) and improved soil properties (pH, cation exchange capacity, nutrient retention). These findings suggest green-synthesized PF-ZrO2 NPs are effective, environmentally friendly candidates for Cr remediation in contaminated soils.

Chemical regulatory authorities require skin sensitization information for a number of chemical sectors, such as cosmetics, pesticides, newly marketed chemicals, household products, and topical pharmaceuticals [...]

One hazardous material that occurs naturally in the environment and induces oxidative stress is cadmium (Cd). Epidemiological data revealed that exposure to cadmium in the workplace and environment might be linked to many illnesses and serious testicular injuries. Aims: It is taught that antioxidants can protect different organs against environmental toxic compounds. Therefore, the current investigation aims to show the role of antioxidants (gallic acid and selenium) in the protection against cadmium toxicity, including the architecture of the testes, semen properties, steroid hormones, protein expression of cytochrome P450 [CYP 19 and 11A1] contributing to the production of steroid hormones, and antioxidant enzyme activities, in male rabbits. Methods: Male rabbits were given cadmium orally three times/week [1 mg/kg BW] for twelve weeks. In addition, gallic acid (20 mg/kg) or selenium (1 mg/kg BW) was administered two hours before cadmium treatment. This investigation included a spectrophotometer, histopathology, and Western immunoblotting techniques. Results: Cadmium treatment significantly reduced sperm counts, testosterone, and estrogen levels after four, eight, and twelve weeks of treatment. In addition, after a 12-week treatment of rabbits with cadmium, the activity of 17β-hydroxysteroid dehydrogenase and antioxidant enzymes, including catalase, superoxide dismutase, glutathione reductase, glutathione peroxidase, and glutathione S-transferase, as well as the glutathione levels, were inhibited in the testes tissue. On the other hand, following cadmium treatment, rabbit’s testes showed a discernible increase in free radical levels. Interestingly, the activity of antioxidant enzymes and level of free radicals were recovered in rabbits treated with gallic acid or selenium before cadmium treatment. In addition, after 12 weeks of cadmium treatment, the steroidogenic protein expressions of CYP 11A1 and CYP 19 were upregulated and downregulated in the testes, respectively. Interestingly, after pretreatment of rabbits with either gallic acid or selenium for two hours before cadmium administration, the downregulated CYP11A1 was restored to normal levels. In the histopathological investigation, immature spermatozoids and sloughed spermatogonium cells were observed in cadmium-treated rabbits’ testes. On the other hand, pretreatments of rabbits with gallic acid or selenium mitigated and alleviated the adverse effects of cadmium on testes architecture and increased the production of healthy sperm. Conclusions: The lower levels of steroid hormones could be due to the downregulation of CYP11A1, inhibition of 17β-hydroxysteroid dehydrogenase, antioxidant enzyme activities, and the induction of free radical levels. Furthermore, the pretreatment of rabbits with gallic acid or selenium mitigated the adverse effects of cadmium on the tissue architecture of testes and steroid hormone levels.

Amidst China’s rapid industrialization and deteriorating air quality, emerging evidence suggests a parallel decline in male reproductive health. However, large-scale assessments of pollution-semen quality associations remain scarce. This nationwide multicenter study investigated these relationships among 27,014 Chinese men using high-resolution satellite-derived exposure estimates (PM2.5, PM10, NO2, O3, CO, and SO2) and generalized linear mixed models (GLMM), adjusting for key demographic confounders. A case-control study involving 5256 cases and 21758 controls used the exposure values of air pollutants 90 days prior to sperm collection for epidemiological exposure analysis reactions to obtain the association between sperm quality and air pollution. This study demonstrates significant associations between increased exposure to regional air pollutants and the risk of substandard semen quality in China. Key findings reveal NO2’s potential reproductive toxicity, showing a 79.7% increased risk of semen volume abnormalities per 11.34 µg/m3 exposure (OR = 1.797, 95% CI: 1.402–2.302). Susceptibility disparities emerged, with 16.4-fold greater PM2.5 sensitivity in obese individuals (OR = 1.121 vs. 1.007) and 133% higher PM10 risk in urban residents (OR = 1.342 vs. 1.006). Strikingly, SO2 exposure at 15% of the WHO 24 h average guideline (6.16 µg/m3) was associated with a 3.8% increase in abnormalities, indicating the challenge of the current safety thresholds. These findings highlight the need for policy reforms, including (1) incorporating reproductive health endpoints into air quality standards, (2) implementing antioxidant interventions for high-risk groups, and (3) strengthening traffic emission controls in urban planning. This study underscores the need for comprehensive strategies to mitigate the impact of air pollution on male reproductive health.

Perfluorooctane sulfonate (PFOS), a persistent organic pollutant, has raised significant public health concerns because of its widespread environmental presence and potential toxicity. Epidemiological studies have linked PFOS exposure to respiratory diseases, but the underlying molecular mechanisms remain poorly understood. Male C57 BL/6J mice were divided into a control group receiving Milli-Q water, a low-dose PFOS group (0.2 mg/kg/day), and a high-dose PFOS group (1 mg/kg/day) administered via intranasal instillation for 28 days. Lung tissue transcriptome sequencing revealed significantly enriched differentially expressed genes in the Ras and Rap signaling pathways. Key genes including Rap1b, Kras, and BRaf as well as downstream genes, such as MAPK1 and MAP2K1, exhibited dose-dependent upregulation in the high-dose PFOS exposure group. Concurrently, the downstream effector proteins MEK, ERK, ICAM-1, and VEGFa were significantly elevated in bronchoalveolar lavage fluid (BALF). These alterations are mechanistically associated with increased oxidative stress, inflammatory cytokine release, and pulmonary tissue damage. The results indicated that PFOS-induced lung injury is likely predominantly mediated through the activation of the Rap1b- and Kras-dependent BRaf-MEK-ERK axis. These findings highlight the critical role of Ras/Rap signaling pathways in PFOS-associated respiratory toxicity and underscore the need to develop therapeutic interventions targeting these pathways to mitigate associated health risks.

This comprehensive review offers new perspectives on the distribution, sources, and ecotoxicological impacts of polycyclic aromatic hydrocarbons (PAHs) in freshwater systems. Unlike previous reviews, this work integrates recent findings on PAH dynamics within environmental matrices and emphasizes spatiotemporal variability across geographic regions. It critically examines both anthropogenic and natural sources, as well as the physical, chemical, and biological mechanisms driving PAH transport and fate. Special attention is given to the ecotoxicological effects of PAHs on freshwater organisms, including bioaccumulation, endocrine disruption, and genotoxicity. Notably, this review identifies key knowledge gaps and proposes an interdisciplinary framework to assess ecological risk and guide effective monitoring and management strategies for the protection of freshwater ecosystems.

The efficient separation and removal of key nuclides is important for the nuclear fuel cycle from the aspects of radioactivity reduction and potential resource recycling. The urgent objective is to design and develop functional materials for the separation and removal of important nuclides. Porous silicon-based adsorbents are considered highly advantageous materials for separating and removing radioactive nuclides in complex environments due to their excellent mechanical properties, high porosity, and functionalization ability. In this review, we compiled the applications of porous silica-based materials in recent years in the separation and removal of key nuclides, such as actinides, lanthanides, strontium, cesium, iodine, and platinum group metals; discussed their separation and removal performances; analyzed the constitutive relationship between key radionuclides and porous silica-based adsorbents; and systematically described the properties and mechanisms of different types of porous silica-based adsorbents. This article aims to provide some ideas for the design of an advanced separation process in the nuclear fuel cycle.

Atmospheric intermediate volatile organic compounds (IVOCs) are important precursors of secondary organic aerosols (SOAs), and in-depth research on them is crucial for atmospheric pollution control. This review systematically synthesizes global advancements in understanding IVOC sources, emissions characterization, compositional characteristics, ambient concentrations, SOA contributions, and health risk assessments. IVOCs include long-chain alkanes (C12~C22), sesquiterpenes, polycyclic aromatic hydrocarbons, monocyclic aromatic hydrocarbons, phenolic compounds, ketones, esters, organic acids, and heterocyclic compounds, which originate from primary emissions and secondary formation. Primary emissions include direct emissions from anthropogenic and biogenic sources, while secondary formation mainly results from radical reactions or particulate surface reactions. Recently, the total IVOC emissions have decreased in some countries, while emissions from certain sources, such as volatile chemical products, have increased. Ambient IVOC concentrations are generally higher in urban rather than in rural areas, higher indoors than outdoors, and on land rather than over oceans. IVOCs primarily generate SOAs via oxidation reactions with hydroxyl radicals, nitrate radicals, the ozone, and chlorine atoms, which contribute more to SOAs than traditional VOCs, with higher SOA yields. SOA tracers for IVOC species like naphthalene and β-caryophyllene have been identified. Integrating IVOC emissions into regional air quality models could significantly improve SOA simulation accuracy. The carcinogenic risk posed by naphthalene should be prioritized, while benzo[a]pyrene requires a combined risk assessment and hierarchical management. Future research should focus on developing high-resolution online detection technologies for IVOCs, clarifying the multiphase reaction mechanisms involved and SOA tracers, and conducting comprehensive human health risk assessments.

Soil contamination by potentially toxic elements (PTEs) poses a major environmental concern. The distribution and concentration of these elements can vary significantly in polluted areas, making detailed assessments crucial. A comprehensive analysis is essential to accurately characterise contamination patterns, as a foundation for effective site evaluation and remediation efforts. This study evaluates the effectiveness and reliability of X-ray fluorescence (XRF) and inductively coupled plasma mass spectrometry (ICP-MS) for determining PTEs in soil samples. Statistical analyses reveal significant differences between the two techniques for Sr, Ni, Cr, V, As, and Zn, likely due to variations in detection sensitivity, calibration methods, or matrix effects. Pb exhibits a weaker difference, suggesting a potential, yet statistically insignificant, difference between methods. Correlation analyses indicate a strong linear relationship for Ni and Cr, while Zn and Sr display high variability, limiting direct comparability. Bland–Altman plots highlight systematic biases, particularly for V, where XRF consistently underestimates concentrations compared to ICP-MS. These findings underscore the importance of selecting the appropriate analytical technique based on detection limits, sample characteristics, and measurement reliability. While both methods provide valuable insights for environmental monitoring, carefully considering their limitations is crucial for accurate contamination assessment.

Lead is a harmful heavy metal known to alter the environment and affect human health. Several industries have contributed to the increase in lead contamination, making it a major global concern. Thus, remediation strategies are necessary to prevent lead bioaccumulation and deleterious health effects. The aim of this study was to determine the capacity of the green microalga Chlorella vulgaris (C. vulgaris or CV) to remove lead in an animal model and prevent the accumulation of this heavy metal in the principal organs (brain, liver, and kidney) and blood. Forty male Wistar rats were randomly assigned to four groups (n = 10): control group (CT); C. vulgaris supplementation group, 5% of the diet (CV); lead acetate administration group, 500 ppm (Pb); and C. vulgaris supplementation group, 5% of the diet plus lead acetate administration group, 500 ppm (CV–Pb). After 4 weeks of exposure, we measured lead accumulation, memory function, oxidative stress, and antioxidant activity (SOD and GSH). Lead exposure altered memory function, increased oxidative stress in the brain and kidney, and increased SOD activity in the brain. Supplementation with C. vulgaris restored memory function to control levels; reduced oxidative stress in the brain and kidney; and decreased the accumulation of lead in the liver, kidney, and blood of rats exposed to lead. Based on our results, C. vulgaris is a lead chelating and antioxidant agent in animal models.

Citrinin (CTN) is a mycotoxin that adversely affects livestock by contaminating stored grains, leading to significant health and economic impacts. This study investigates the toxicological effects of CTN on porcine small intestinal epithelial cells (IPEC-J2) and explores potential mitigation strategies using natural products and chemical inhibitors. Our study demonstrates that CTN induces cytotoxicity through the TGF-β signaling pathway, triggering apoptosis and G2/M phase cell cycle arrest. We examined cell viability, cell cycle progression, and gene expression changes in IPEC-J2 cells treated with CTN, 4′-Hydroxydehydrokawain (4-HDK), and LY-364947, a TGF-β receptor inhibitor. LY-364947 treatment confirmed that CTN-induced toxicity is mediated through TGF-β signaling. Although 4-HDK alleviated CTN-induced cytotoxicity by improving cell viability and reducing apoptosis, its direct involvement in TGF-β inhibition remains unclear. These results suggest that CTN disrupts intestinal epithelial cell homeostasis via TGF-β activation, whereas 4-HDK may exert protective effects through an alternative mechanism. Our study provides novel insights into CTN-induced toxicity mechanisms and highlights the therapeutic potential of 4-HDK as a mitigator of mycotoxin-induced cellular damage.

Polybrominated diphenyl ethers (PBDEs) are synthetic halogen compounds, industrially used as flame retardants in many flammable products. PBDEs are environmentally persistent and bioaccumulative substances that were used from the 1970s and discontinued in the 1990s. PBDEs are present in air, soil, water, and food, where they remain stable for a long time. Chronic exposure to PBDEs is associated with adverse human health effects, including cancer, immunotoxicity, hepatotoxicity, reproductive and metabolic disorders, motor and hormonal impairments, and neurotoxicity, especially in children. It has been demonstrated that PBDE exposure can cause mitochondrial and DNA damage, apoptosis, oxidative stress, epigenetic modifications, and changes in calcium and neurotransmitter levels. Here, we conduct a comprehensive review of the molecular mechanisms of the neurotoxicity of PBDEs using different approaches. We discuss the main neurotransmitter pathways affected by exposure to PBDEs in vitro and in vivo in different mammalian models. Excitatory and inhibitory signaling pathways are the putative target where PBDEs carry out their neurotoxicity. Based on this evidence, environmental PBDEs are considered a risk to human public health and a hazard to biota, underscoring the need for environmental monitoring to mitigate exposure to PBDEs.

This study analyzed the factors influencing personal PM2.5 exposure levels among adults with allergic diseases in Seoul using a linear mixed-effects (LMEs) model. The average personal PM2.5 exposure concentration of the study participants was 17.38 μg/m3, exceeding the World Health Organization (WHO) daily recommended guideline (15.00 μg/m3), though it was relatively low compared to global levels. Inter-individual exposure variability was approximately 43.5%, with exposure levels varying significantly depending on microenvironments. Notably, 58% of participants exhibited higher exposure on weekends compared to weekdays, likely associated with increased outdoor activities. The LMEs model results identified smoking (90.81% higher in smokers), temperature, relative humidity, outdoor pollutants (PM2.5, O3, CO), indoor PM2.5 and CO concentrations, and time spent in residential environments as factors increasing exposure, while rainfall (91.23% reduction), wind speed, and air purifier use were identified as factors reducing exposure. These findings suggest that individual activity patterns and environmental factors significantly influence exposure levels, highlighting the need for personalized mitigation strategies and national fine dust policies. This study is expected to provide scientific evidence contributing to the reduction in health risks and improvement of quality of life for individuals with allergic diseases.

Tryptophan (Trp) is an important essential amino acid that plays a variety of physiological functions in the human body, including being a precursor of neurotransmitter and participating in immune regulation. Currently, more and more studies show that some pollutants in the environment can affect the metabolism of Trp and consequently affect human health. The present paper offers a comprehensive overview of prior research investigating the impact of environmental pollutants, including inorganic and organic contaminants, microplastics, and nanoplastics on the nervous system, immune system, digestive system, and maternal–fetal pregnancy, revealing their detrimental effects on Trp metabolism and human well-being.

To clarify the heavy metal(loid) contamination characteristics and health risk in nine typical greenhouse production areas in Jingmen, Central China, the total concentrations of As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn in 176 soils and 332 vegetables were analyzed. Cadmium (100%) and Ni (4/44) exceeded the Chinese standard limits, while As (36/44), Cd (36/44), Cr (9/44), and Ni (1/44) exceeded the international soil quality standards. The As, Cd, Cr, Ni, and Pb contents in all vegetables were over both the domestic and international standard limitations. The soil pollution levels of Hg and Cd and the potential ecological risk in Zhanghe and Lishi require more attention. Significant levels of Cu, Pb, and Cr in the soil and Hg, Ni, and Cu in edible vegetable parts were suggested to be mainly caused by daily agricultural production management. Although non-carcinogenic and carcinogenic risks to vegetable consumers and greenhouse workers are acceptable across different age groups, more scientific management and remediation must be carried out simultaneously for sustainable production in the future, especially in GD and ZH. Updated standard values for the environment and food, together with the 2023 updated soil geochemical background values, should be applied in time.

Metformin disinfection byproducts Y and C have emerged as pollutants of concern in drinking water systems and are suspected to possess significant toxicity to mammals. However, effective strategies to mitigate the effects of Y and C exposure in mammals have not been thoroughly formulated. This study aimed to investigate the toxicity and characteristic phenotypes of short-term, high-dose exposure to Y and C in the intestine and liver of mice and to evaluate the protective effects of Dictyophora rubrovolvata extract (DRE) on Y- and C-induced intestinal and liver damage. The results showed that exposure-induced intestinal toxicity manifested mainly as intestinal barrier dysfunction, induction of immune response and oxidative stress, and disruption of intestinal flora homeostasis. Hepatotoxicity was mainly characterized by histopathological changes such as vacuolar degeneration, abnormal liver function, and oxidative stress. Additionally, marked changes in gut microbiota and biochemical indicators were closely related to hepatic and intestinal injuries after exposure. DRE effectively alleviated Y- and C-induced intestinal and liver damage, reshaped the gut microbiota, and maintained gut–liver axis homeostasis. These findings provide new insights into the toxic effects of disinfection byproduct exposure through the gut-liver axis and suggest that functional food extracts may serve to protect against these adverse health outcomes.

The optimal descriptors generated by the CORAL software are studied as potential models of cardiotoxicity. Two significantly different cardiotoxicity databases are studied here. Database 1 contains 394 hERG inhibitors (pIC50) and external 200 substances that are potential drugs, which were used to confirm the predictive potential of the approach for Database 1. Database 2 contains cardiotoxicity data for 13864 different compounds in a format where active is denoted as 1 and inactive is denoted as 0. The same model-building algorithms were applied to all three databases using the Monte Carlo method and Las Vegas algorithm. The latter was used to rationally distribute the available data into training and validation sets. The Monte Carlo optimization for the correlation weights of different molecular features extracted from SMILES was improved by including the conformity coefficient of the correlation prediction (CCCP). This improvement provided greater predictive potential in the considered models.

Road-deposited sediments (RDS) from 28 sites in Beijing were studied and analyzed for eight heavy metals. In RDS, the levels of Cr, Ni, Cu, Zn, As, Cd, Pb and V were 2.76, 1.11, 2.40, 1.65, 1.09, 6.52, 4.13 and 0.06 times the background values, respectively. The levels were rated in accordance with the geoaccumulation index (Igeo) as follows: Cd>Pb>Zn>Cu>Cr>Ni>As>V. In the four functional zones, the potential ecological risk index method showed that most of the heavy metals have environmental risk index values (Eri) of less than 40, but the multifactor environmental risk value (RI) for Pb in the transportation area exceeded 150. Four functional areas had Cd values greater than 160, exhibiting the highest risk. The human health risk assessment revealed that exposure pathways followed this decreasing order: ingestion > dermal > inhalation. Three DOM fractions were resolved in the sediments of the four functional zones, including terrestrial fulvic-acid-like fractions (C1), humic-acid-like fractions (C2), and tryptophan-like fractions (C3), and the DOM fractions were affected by both exogenous and endogenous sources. A positive correlation existed between DOM and Ni and Cu in the transportation zone, and the correlation between DOM and heavy metals in other zones was not apparent. In conclusion, heavy metals in different functional zones affected the concentration and characteristics of DOM, and there was a strong correlation between heavy metals and DOM concentration and features.

During the natural cooling process of magnesium slag stockpiles in the open air, the phase transformation of gamma-dicalcium silicate (γ-C2S) induces a powdering phenomenon, resulting in the generation of a large amount of PM10 and PM2.5 dust. Based on the dust emission model of stockpiles and the Gaussian dispersion model, combined with the Monte Carlo simulation method, this study conducted a quantitative assessment of the environmental risk of heavy metals (Pb, Cd, Hg, As, Cr(VI)) in dust to the surrounding residential areas. The results show that the enrichment degree of heavy metals in PM2.5 is significantly higher than that in PM10. At a downwind distance of 1000 m, the exceedance multiples of Cr(VI), As, and Cd reach 131.5, 23.6, and 51.8 times, respectively. The total carcinogenic risk (9.2 × 10−7) and total non-carcinogenic hazard quotient (0.15) in the residential area are below the limits, but the contribution rates of As and Cd are relatively high. Sensitivity analysis further reveals that the moisture content of the stockpile, dust removal rate, and distance are the key control parameters affecting the environmental risk. Based on the research findings, it is recommended to increase sprinkling frequency, install windbreak nets, and promote magnesium slag utilization to effectively control dust risks.

Bacillus subtilis exhibits a great affinity to soluble U(VI) through non-reducing biomineralization. The pH value, temperature, initial uranium concentration, bacterial concentration, and adsorption time are recognized as the five environmental sensitive factors that can regulate the degree of non-reductive biomineralization. Most of the current studies have focused on the regulatory mechanisms of these factors on uranium non-reductive mineralization. However, there are still few reports on the importance of these factors in influencing non-reductive mineralization, as well as on how to regulate these factors to increase the efficiency of non-reductive mineralization and enhance the enrichment of Bacillus subtilis on uranium. In this project, a DLNN deep learning neural network model was constructed to effectively predict the effects of changes in these five environmental sensitivity factors on the non-reducing mineralization of Bacillus subtilis to uranium. Accuracy (99.6%) and R2 (up to 0.89) confirm a high degree of agreement between the predicted output and the observed values. Sensitivity analysis shows that in this model, pH value is the most important influencing factor. However, under different pH values, temperature, initial uranium concentration, adsorption time, and bacterial concentration have different effects. When the pH value is lower than 6, the most important factor is temperature, and once the pH value is greater than 6, the initial concentration is the most important factor. The results are expected to provide a theoretical basis for regulating the enrichment degree of U(VI) by Bacillus subtilis, achieving the maximum long-term stable fixation of U(VI), and understanding the environmental chemical behavior of uranium under different conditions.

Air pollution exposure has been increasingly linked to adverse pregnancy outcomes. This study aimed to investigate the effects of PM2.5 exposure throughout pregnancy on preterm birth, low birth weight (LBW), and small for gestational age (SGA). We analyzed a cohort of 16,965 pregnant women living in northern Thailand between 2016 and 2022. PM2.5 concentration data were collected from two air quality monitoring stations operated by the Pollution Control Department (PCD) of Thailand. Logistic regression models were used to assess the association between daily PM2.5 exposure and pregnancy outcomes. PM2.5 exposure at levels exceeding 37.5 μg/m3 throughout pregnancy significantly increased the risk of preterm birth (aOR = 2.19, p < 0.001) and LBW (aOR = 1.99, p < 0.001) compared to the reference group (15.1–37.5 μg/m3). However, exposure at levels ≤15.0 μg/m3 also increased the risk for both outcomes compared to the same reference group. Subgroup analysis of high-risk pregnant women, including women aged > 35 years, with pre-pregnancy BMI (<18.5), pregnancy-induced hypertension, and nulliparous women, showed that the range of the critical PM2.5 exposure threshold was 32.3–38.4 μg/m3 for preterm birth and 31.2–38.2 μg/m3 for LBW. This study highlights the significant association between PM2.5 exposure and adverse pregnancy outcomes and suggests the need for targeted interventions to mitigate its effects on maternal and child health.

The present study investigated the uptake and health risks of potentially toxic elements (PTEs) by lotus (Nelumbo nucifera) cultivated in floating lake gardens of Dal Lake, Srinagar, India. Rapid urbanization and anthropogenic activities have led to PTE contamination in the lake, raising concerns about food safety and ecological sustainability. The objectives were to evaluate the physicochemical properties of water and sediment and to quantify PTEs (Cd, Cu, Cr, Co, Fe, Mn, Ni, and Zn) accumulation in different tissues of N. nucifera with associated health risks. A systematic sampling approach was adopted across four zones of the lake, collecting water, sediment, and plant tissues (August to October 2024). The results showed significant PTE contamination, with sediment showing high concentrations of Fe (1610.51 mg/kg), Mn (31.48 mg/kg), and Cr (29.72 mg/kg). Bioaccumulation factors indicated preferential PTE accumulation in roots, with Fe exhibiting the highest uptake (95.11). Translocation factors were low for most PTEs, suggesting limited mobility to edible parts. Health risk assessment indicated that Cr posed the highest non-carcinogenic risk (HRI = 1.8000 in rhizomes). The cumulative target hazard quotient (THQ) remained below 1, suggesting no immediate risk, but long-term exposure warrants concern. The study provided valuable information on the necessity of continuous monitoring and pollution mitigation strategies to ensure the food safety of floating lake garden-derived crops.

Polycyclic aromatic hydrocarbons (PAHs) and global warming impact aquatic ecosystems, eventually interacting. Monolayer (2D) cultures of cell lines, such as the rainbow trout liver RTL-W1, are employed for unveiling toxicological effects in fish. Nonetheless, three-dimensional (3D) models constitute an alternate paradigm, better emulating in vivo responses. Here, ultra-low attachment (ULA) plates were used to generate ten-day-old RTL-W1 spheroids for exposure to a control, a solvent control (0.1% DMSO) and the model PAH benzo[k]fluoranthene (BkF) at 10 and 100 nM and at 18 and 23 °C (thermal stress). After a 4-day exposure, spheroids were analyzed for viability (alamarBlue and lactate dehydrogenase), biometry (area, diameter and sphericity), histocytology (optical and electron microscopy), and mRNA levels of the detoxification-related genes cytochrome P450 (CYP)1A, CYP3A27, aryl hydrocarbon receptor (AhR), glutathione S-transferase (GST), uridine diphosphate–glucuronosyltransferase (UGT), catalase (CAT), multidrug resistance-associated protein 2 (MRP2) and bile salt export protein (BSEP). Immunocytochemistry (ICC) was used to assess CYP1A protein expression. Neither temperature nor BkF exposure altered the spheroids’ viability or biometry. BkF modified the cell’s ultrastructure. The expression of CYP1A was augmented with both BkF concentrations, while AhR’s increased at the higher concentration. The CYP1A protein showed a dose-dependent increase. Temperature and BkF concurrently modelled UGT’s expression, which increased in the 100 nM condition at 23 °C. Conversely, CYP3A27, MRP2, and BSEP expressions lowered at 23 °C. CAT and GST mRNA levels were uninfluenced by either stressor. Overall, BkF and temperature impacted independently or interactively in RTL-W1 spheroids. These seem to be useful novel tools for studying the liver-related effects of temperature and PAHs.

The rise of multidrug-resistant (MDR) Pseudomonas aeruginosa (P. aeruginosa) presents a significant challenge to clinical treatment and environmental risks. This review delves into the complex mechanisms underlying MDR development in P. aeruginosa, such as genetic mutations, horizontal gene transfer (HGT), and the interaction between virulence factors and resistance genes. It evaluates current detection methods, from traditional bacteriology to advanced molecular techniques, emphasizing the need for rapid and accurate diagnostics. This review also examines therapeutic strategies, including broad-spectrum antibiotics, novel drug candidates, combination therapies, and innovative approaches like RNA interference, CRISPR-Cas9 gene editing, and bridge RNA-guided gene editing. Importantly, this review highlights the distribution, migration, and environmental risks of MDR P. aeruginosa, underscoring its adaptability to diverse environments. It concludes by stressing the necessity for continued research and development in antimicrobial resistance, advocating for an integrated approach that combines genomics, clinical practice, and environmental considerations to devise innovative solutions and preserve antibiotic efficacy.