The Indian journal of animal sciences

Online ISSN: 0367-8318
Material from 13 cases of abortion in 1 epidemic outbreak of abortion among mares was pooled into 3 lots and injected intraperitoneally into 3 separate sets of mice. The mice died within 5-7 days. Tissues from each set of inoculated mice showed the presence of yeast-like bodies, and pooled material from each of the 3 lots also yielded the yeast in culture. Histopathological examinations of the mare placenta and fetal organs also revealed the presence of the yeast. The organisms appeared round or oval, thin-walled, and about 2-4 microns in diameter and were identified as Candida pseudotropicalis. Urease test was negative. Acid and gas formation was found within 3 days of incubation at 27 degrees C in glucose, lactose, and sucrose, but not in maltose.
Entoviruses and Mycoplasma bovigenitalium were isolated from 37 bovine abortions and characterized. Enteroviruses were isolated in 2 cases and Mycoplasma bovigenitalium in 1 case. Characteristics of the isolates of enterovirus were: resistance to ether and chloroform, lability at 56 degrees C for 30 minutes, resistance to pH 3.2 buffer, cationic stabilization in the presence of M MgC1(2) solution, and neutralization by La Placa group 2 antisera. Morphological, biochemical, serological, and epi-immunofluorescent methods were used in the identification of Mycoplasma bovigenitalium. The etiologic role of these agents in bovine abortions is discussed.
In Rajasthan, India megastrol acetate and progesterone were administered to ewes (25 Chokla, 25 Malpura, aged between 3 and 4) in November 1968. Each ewe received 50 mg of either megastrol acetate or progesterone. Administration was intravaginal or intramuscular. During treatment only 3 ewes (2 megastrol acetate-injected and 1 progesterone-injected ewes) came into heat; thus, the progestogens exerted an inhibiting effect. 76% and 88% of the megastrol acetate and progesterone ewes, respectively, exhibited heat within 96 hours of therapy's end. Breed showed no significant effect on occurrence of estrus after therapy. Release of estrus within 96 hours occurred in 91.3% and 74.0% of intravaginally and intramuscularly treated ewes, respectively. 17 (41.5%) of the 41 ewes inseminated at their synchronized estrus lambed as a result of breeding in that first cycle. Thus, questions arise as to efficiency of ovulation, fertilization, nidation, and lambing in the case of synchronized estrus by use of different progestogens. 75% of ewes showing typical fern pattern at insemination lambed. An antiseptic vaginal douche applied after withdrawal of the sponge used in iv administration might be responsible for increased posttreatment fertility in this group.
Top-cited authors
Mohinder Bakshi
  • Guru Angad Dev Veterinary and Animal Sciences University
Madhu Sudan Tantia
  • National Bureau of Animal Genetic Resources
Manju Wadhwa
  • Guru Angad Dev Veterinary and Animal Sciences University
S. D. Kharche
  • Central Institute for Research on Goats
Dhirendra Singh
  • Central Sheep and Wool Research Institute