Research Journal of Pharmacy and Technology

Print ISSN: 0974-3618
A simple, specific, sensitive, precise stability-indicating high-performance liquid chromatography method for determination of Calcipotriene was developed and validated. A Zorbax 300 SB-C18 column (250*4.6 mm, 3.5 μ) in isocratic mode, with mobile phase consisting of a mixture of solution methanol: water (70:30) was used. The quantitation performed at flow rate of 1.0 mL/min at 264 nm and run time was 7.5 min. The analytical method was validated as per ICH guideline for linearity, accuracy, precision, specificity, limit of detection, limit of quantification, and stability and method can be extended to the analysis of Calcipotriene in semis olid formulations. The relative standard deviation values for precision was less than 2%, and % recovery was greater than 98% for Calcipotriene. The drug is photo and thermal sensitive, so the analysis should be done in moderate conditions only.
Introduction: Nosocomial infections are caused by the retrograde spread of viral, bacterial and fungal pathogens from the hospital environment to the patients. Despite the efforts to avoid cross-infection in the dental office using sterilized instruments, individual protection equipment, and disinfection procedures, there is an increased risk of cross-infection through dental units, since one dental chair is used to treat many patients. Materials and methods: Seven strains of micro-organisms- Streptococcus, Lactobacillus, Pseudomonas aeruginosa, Klebsiella, Enterococcus, Staphylococcus aureus, Candida albicans, E. coli, and Legionella were taken. Neem extract, Aloe Vera Juice, and 0.2% chlorhexidine were tested against all these organisms. Sterile discs were incorporated with an equal amount of prepared formulations using a micropipette. Then these discs were placed equidistant to each other following which these plates were incubated for 24 hours. Results: Zone of inhibition was higher in chlorhexidine 12mm, 22mm, 11mm, 8mm, 18mm, 12mm, 11mm, 14mm and 15 mm followed by neem extract 7mm, no inhibition, 10mm, 7mm, 11mm, 7mm, 11mm and 9 mm and no inhibition by Aloe Vera juice against Streptococcus, Lactobacillus, Pseudomonas aeruginosa, Klebsiella, Enterococcus, Staphylococcus Aureus, Candida albicans, E.coli and Legionella respectively. Conclusion: Results from this study have shown that chlorhexidine was most effective against dental unit waterline pathogens followed by neem extract and least by Aloe Vera juice.
Effect of S. schimperiana root chloroform extracts (SSRCH-200 and SSRCH-400 mg/kg bw) treatments on percentage of neutrophil adhesion. Each group consists of n=5 animals. Values are Mean ± S.E.M. P<0.05 is considered as significant. **P<0.01, *P<0.05 as compared to control group and Standard
Objective: The present study was aimed to evaluate the in vivo immune protective potential of chloroform root extract of Sida schimperiana on E.coli 018:K1 induced peritonitis in albino Wistar rats. Methods: Acute toxicity of was performed by oral administration of S. schimperiana chloroform root extract (SSRCH) 5, 50, 300 and 2000mg/kg body of male albino Wistar and mortality was monitored for 14 days. Based on LD50, 1/10th, 1/5th cut-off values of the (SSRCH) plant extract was selected as a dose for E. coli induced peritonitis in albino Wistar rats. Wistar rats were pre-treated with 200 and 400mg/kg/bwt of SSRCH and Standard antibiotic Ofloxacin 5mg/Kg body weight was given oraly for a period of 7 days. The dosing regimens were started on day -1,-2, -3, 0, 1, 2 and 3 relative to the day of challenge (day 0) with 2×104 CFU of E. coli CFU/ml (i.p.) and mortality was monitored for 14 days. After the monitoring the mortality, the treated (Groups I-IV) rats were sacrificed, and assess the in vivo antibacterial activity of S. schimperiana chloroform root extract by determination of CFU/ml in peritoneal lavage fluid. Further SSRCH extract (400mg /kg bw) was analyzed by the neutrophil adhesion in Wistar rats for evaluated immunomodulatory activity. Results: In acute toxicity studies no mortality was observed for 24-48 hours. SSRCH extract 400mg (Group-IV) showed protection against E. coli induced peritonitis in albino Wistar rats by showing 60% survivability and also exhibited significantly increased percentage of neutrophils adhesion. i.e (25.74±2.351and 36.14±5.609) at 200 and 400mg/kg respectively. Conclusion: The present study concluded that the chloroform root extract of S. schimperiana had a significant amount of polyphenolic compounds and could serve as a potential source of natural antibacterial and Immunomodulatory agents for the development of therapeutic antibiotics with immunostimulatory activity in the treatment of intraperitoneal infections.
The present research work describes the development of a new simple, precise, sensitive and validated RP-HPLC method for Zn-sparfloxacin 1,10-phenanthroline metal complex. The chromatographic conditions used for the separation was Phenomenex Luna C18 (250mm × 4.6mm, 5μm particle size) and mobile phase comprised of methanol: phosphate buffer (30:70 v/v) and pH was adjusted to 3 by o-phosphoric acid. The flow rate was 1.0 ml/min with detection at 293 nm. The retention time was found to be 4.357 min. The linearity was found to be in the range of 2-10 μg/ml for Zn-spar-phe metal complex with correlation coefficient of 0.9996. The proposed method is accurate with 98-102 % recovery and precise (%RSD of repeatability, intra-day and inter-day variations were 0.240, 0.7989- 1.1386 and 1.09-1.53 respectively). The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.2658 and 0.8056 μg/ml respectively. Proposed method was validated as per ICH guidelines for precision, accuracy and linearity for estimation of Zn-spar-phe Metal complex. Results of the validation were found satisfactory.
Pseudomonas aeruginosa, a Gram-negative human pathogen, P. aeruginosa is lone of the furthermost common hospital pathogens also is a chief concern, particularly in immune-compromised patients. The purpose of this study was to identify phenotypic and genotypic antibiotic resistance in Pseudomonas aeruginosa isolated from wound infection The bacterial isolates (30) were obtained from patients admitted to Mirjan Medical City in Babylon, Iraq (burns, wound unit). was identified biochemically and morphologically, and the isolates were subjected to standard bacteriological culturing processes on blood and MacConkey agar plates for 24-48 hours at 37oC for isolation and purification, Viteck 2 compact system confirmed the isolates and antibacterial sensitivity as well. These findings revealed that P.aeruginosa has a high rate of penicillin resistance, with a resistance rate of (100percent ) isolates. Advanced resistance to cephalosporin antibiotics was also found in resistant isolates of Cefoxitin, Ceftriaxone (75%), Ceftazidime, and cefepime (85%). For carbapenem antibiotics, had a high resistance rate (90percent). Aminoglycosides have variable resistance to Amikacin (60percent), Gentamicin (70 percent), and tobramycine resistant (90%). The findings revealed that all Pseudomonas aeruginosa isolates tested positive for Class 1,2 Integron resistance genes. with positive results (35%) for Int1 and 35% for Int2 (25%). This study found that P.aeruginosa has a high rate of resistance to Penicillins, Cephalosporin, Carbapenem and Aminoglycosides antibiotics.
General scheme for synthesis of dihydrobenzimidazoqunazolines (3a and 3b). 4-(5,6-dihydrobenzo[4,5]imidazo[1,2-c]quinazolin-6-yl)-2-ethoxyphenol (3a): Following the microwave-assisted method (3.3.1) using 3-ethoxy-4-hydroxybenzaldehyde (0.2 g, 1.2 mmole), new compound (3a) was obtained and characterized directly without any further purification as a white solid (0.35 g, 97%), while following the conventional heating method (3.3.2) the same compound was obtained in 0.32 g, 92% ; m.p.: 219-221°C; Rf: 0.63 in hexane: ethyl
The inhibitory logarithmic concentration of compound (3b) against Hep G2 cells; * Hep G2: Hepatic cancer cells; *WRL68: Normal cell line as a control.
High content screening images of human Hep G2 cells treated with the potential anticancer compound (3b) and doxorubicin in comparison to untreated cell culture using multiparameter which included: nuclear intensity, cell membrane permeability, mitochondrial membrane permeability (MMP), and cytochrome C.
The 1 H NMR spectrum for compound (3a) is demonstrated in Fig. 2, while the spectrum for compound (3b) is intensively described in the Supplementary material file 2a.
Absorption and emission maxima and quantum yields (Φ)
Pursuing our interest in bioactive heterocyclic compound, two benzoimidazoquinazoline derivatives were synthesised using both microwave-assisted and classical heating methods. Structures of the compounds were confirmed by standard spectroscopic methods and elemental analysis. The target scaffolds were incidentally found to emit blue light when exposed to ultraviolet light. Consequently, a photoluminescence characterization was carried out as a part of characterization protocol. The anticancer activities of the benzimidazoquinazoline compounds were investigated using both methylthiazol tetrazolium (MTT) and the high content screen (HCS) assays against liver hepatocellular cells. The results showed a significant reduction in the inhibitory concentration of the cancer cells by 1 and 2.6 fold when exposed to compounds (3a) and (3b), respectively. The high content screen (HCS) was conducted for compound (3b) and the results showed high toxicity towards the cancer cells.
F.T.I.R Spectrum of Compound [A].
F.T.I.R Spectrum of Compound [B1]
1 H.NMR Spectrum of Compound [AB1]
Antibacterial activity of some derivative compounds against pseudomonas aeruginosa
This study aimed to prepare some new 1,2,3,4-Tetrazole derivatives from o-tolidine as a starting materials and to detection antibacterial activity against two type of pathogenic bacteria (Staphylococcus aureus and Klebsiella pneumoniae) isolated from patients infected with burns infections: The first step includes formation of new Schiff bases derivatives from o-tolidine [A] concentrated with many aromatic aldehydes [4-chlrobenzaldehyde, 4-bromobenzaldehyde, p-dimethyle amino benzaldehyde] in absolute ethanol to give [A1-A3]. The second step includes synthesis [B1-B3] by diazotation of [1-(4-aminophenyl) ethanone, 4-amino-N-(2H-imidazol-2-yl) benzenesulfon amide, 4-nitroaniline], respectively. The formed salt was treated with sodium azide, ethyl acetoacetate and acetylacetone, respectively. [B1-B3] Compounds were entered in 1, 3- dipolar cycloaddition reactions with [A1-A3] in dimethylformamide to give 1, 2, 3, 4-Tetrazole derivatives [AB1-AB3]. Antibacterial activity of nine derivative compounds were done against two types of pathogenic bacteria (p. aeruginosa and S. aureus). Results of this study proved that there were significant bacterial inhibition against p. aeruginosa and S.aureus with inhibition zone 20 mm and 15 mm, respectively.
Research on benzimidazole and thiazolidine-4-one and their synthetic analogs have been revealed to posses wide range of antimicrobial activity. It was our interest to make novel derivatives of fused benzimidazole, thiazolidine-4-one nuclei and evaluate them for antimicrobial activity. Formic acid was treated with o-phenylenediamine to obtain benzimidazole which was treated with CH 3I for N-methylation. Later this compound was subjected for Vilsmeier-Haack Reaction, followed by formation of Schiff bases on treatment with various aromatic amines. The various Schiff bases were further treated with thioglycolic acid to obtain thaizolidine-4-one derivatives. (Scheme-I). In another (Scheme-II) N-methyl substituted benzimidazole-2-carbohydrazide (Vilsmeier-Haack Reaction product) followed by acidification, esterification and hydrazide formation. Finally hydrazide treated with different aldehydes to obtain carbohydrazide derivatives. The structures of synthesized derivatives were identified and characterized by m.p, TLC, IR, 1HNMR and Mass Spectroscopy. The synthesized compounds BA-SA, BA-SN, AH-BR and AH-CL have shown significant antibacterial activity against E. coli, E.fecalis, Klebsiella and S. aureus and BA-SA, BA-SN, BA-CL and AH-CL shown significant antifungal activity against C. albicans and A. niger.
Drug discovery and development is a multidisciplinary, creative, innovative and highly regulated process. Finding a successful lead has been a great challenge in pharmaceutical research and is utmost important in the formulation development. Optimization of the process used to manipulate the compound to improve its chemical stability, potency, biological or therapeutic effectiveness. Literature survey reveals 1,2,4-oxadiazoles are statistically significant in bioorganic and pharmaceutical chemistry. They were known to exhibit varied pharmacological properties. This review article provides up to date information about developments, exploration of new methods, techniques adopted for the synthesis of 1,2,4-oxadiazoles, their transformation to biologically important derivatives, and studies on their pharmacological properties.
Compounds containing 1,2,4-triazole moiety find wide range of applications and substituted 1,2,4-triazoles are becoming more prevalent in functional materials that are at the cutting edge of new technology. The biological activities of 1,2,4-triazole derivatives are well documented and important discoveries in this area are continued, though the electron donor and coordinating ability of 1,2,4-triazoles has also seen an increase in their use as ligands, functional polymers, and industrial coatings. 1,2,4-triazole has shown its importance as antimicrobial, antiinflammatory, hypoglycemic, antidepressant, antitubercular, analgesic, antimalarial and anticancer agents. This article is based on different pharmacological activities exhibited by the various compounds containing mercapto and/or thione substituted 1,2,4-triazole heterocyclic ring systems. This review highlighted the basic pharmacophoric requirements for the successful development in the designing of novel antibacterial, antifungal, antitubercular, and antiinflammatory mercapto triazole agents.
Some characteristics of compounds (4a-g) and (5a-g)
The activities of benzoxazepines (5a-g) athwart bacteria
Treatment of 4-aminobenzoyl hydrazide (1) with (CS2) and potassium hydroxide in absolute (EtOH) resulted in formation of 5-(4-aminophenyl)-2-thiol-1,3,4-oxadiazole (2). Compound (2) has been converted to the diazonium salt which reacted with 2-hydroxybenzaldehyde for producing the aldehyde derivative (3). Reaction of compound (3) with (4-nitroaniline, 3-nitroaniline, 2-nitroaniline, 4-chloroaniline, 2-chloroaniline, 2,4-dichloroaniline and 4-bromoaniline) by (MW) method in (EtOH) afforded seven Schiff bases (4a–g). Cycloaddition of imines (4a–g) with phthalic anhydride in microwave oven gave seven 1,3-benzoxazepine-1,5-diones (5a–g) bearing oxadiazole moiety. Screening verves of final benzoxazepines was done on Staphylococcus aurous and Escherichia coli. The implications explained that compounds (5b, 5c, 5d, 5e, 5f and 5g) possess higher effect than gentamycin against Staphylococcus aurous. Moreover, the 1,3-oxazepine-1,5-diones (compounds 5a, 5b, 5c, 5d, and 5g) appeared better action against Escherichia coli comparably with the standard antibiotic.
Recently a series of 1-methylene-4-(5-(4-(phenylamino)phenyl)-1,3,4-thiadiazol-2-yl)thiosemicarbazide derivatives were synthesized and their pharmacologically evaluated for antimicrobial activities. The purpose of this study was to evaluate the potency of the title compounds on bacterial and fungal activities by varying the substituted in the 1- methylene-4-(5-(4-(phenylamino)phenyl)-1,3,4-thiadiazol-2-yl)thiosemicarbazide derivatives. All compounds of this series showed promising antimicrobial activities. It was found that some of these compounds possess marked antifungal and antibacterial properties comparable in efficiency to the reference drug Flukanazole and Ciprofloxacin respectively. In present study the structures of compounds were confirmed by UV, IR, 1H NMR.
The newly synthesised ligand 4-(4-(dimethylamino)phenyl)-1,3,6-triphenylpiperidin-2-one [DMP] with phenyl united piperidine moieties within the main cyclic chain was synthesized through the Michael addition reaction and qualitative analysis was characterised by FT-IR, NMR (13C, 1H). The present work deals with the computational analysis of a synthetic compound as a ligand with anticancer activity. The molecule is analysed for its druggable property and biological significance using several softwares. The molecule was docked with the receptor protein bearing the PDB ID 1UNG. The results of the anti-cancer of synthesised ligand DMP are correlated with the docking calculations performed on protein Cyclin-dependent kinase 5 using ligand fit protocol available through Acclerys Discovery studio 2.1
N. nucifera Gaertn Seed Powder was extracted with 50% ethanol, anti oxidant and hepatoprotective activities of the extract was evaluated in 1,4 Dichlorobenzene intoxicated rats. In this study, changes in liver marker enzymes (AST, ALT and ACP) present in serum, antioxidant enzymes (SOD, CAT, GST and GSH) and plasma lipid profile (HDL, LDL, VLDL, TG and Total Cholesterol) were investigated in 1,4 DCB intoxicated rats (300 mg / kg of body wt) to determine its role in toxicity. After treating with 100 mg/kg body wt of N.nucifera Gaertn seed extract, serum enzymes, antioxidant enzymes and plasma lipids return to its normal level in the 1, 4 DCB intoxicated rats. This seed extract showed its best antioxidant potential and liver protective effect like standard drug silymarin.
The α,β unsaturated ketone 3-(2,4-dimethyl-1H-pyrrol-3-yl)-1-phenylprop-2-en-1-onederivatives were treated with benzene-1,2-diamine to obtain 2-(2,4-dimethyl-1H-pyrrol-3-yl)-4-phenyl-2,3-dihydro-1H-benzo[b][1,4]diazepine derivatives. These synthesized compound were characterized by IR, 1H NMR, and mass spectroscopy. These synthesized molecules were evaluated for invitro antimicrobial activity. All The synthesized compounds, showed potent anti-microbial activity as compare to reference drug. In these study the synthesized were docked with Type IIA topoisomerases 2XCT using glide dock program and binding affinity were predicted for the synthesized compounds. The compound AP8 and AP9 have shown more active as per binding energy.
The main objective of this work is to determine the effect of the harvest period on the yield and chemical quality of the essential oils of the Eucalyptus sideroxylon A. Cunn. leaves of the Mamora forest, Dayet Zerzour Bnifdel region, Rabat. Essential oil yields are remarkably high above 2.3%, with an ultimate rate of 5.48% for the month of April. The chemical quality of these essential oils is characterized by the presence of two major monoterpenes, 1,8-cineole (eucalyptol) and α-terpineol, which are proportionally inverted in terms of quantity. During the wet months between September and March, the cineole predominates with levels ranging from 72.67% to 86.11% and the other dry months are characterized by an increase of α-terpineol rate from 12.05% to 25.61%. This inverted chemical variability reveals a change in the orientation of cineole and α-terpineol biosynthesis under the control of climatic factors. This work allows us to discern favorable periods for the harvest of Eucalyptus sideroxylon leaves in terms of yield and quality of essential oils.
Triple-negative breast cancer (TNBC) remains as the deadliest cancer type due to the lack of treatment options. Hence, several attempts have been made to develop new anticancer for TNBC therapy. This study intended to challenge curcumin analog (CCA)-1.1, which is derived from pentagamavunone-1 structure, against the 4T1 cell line and TNBC cell model, covering the cytotoxic activity in correlation with cell cycle progression, apoptosis induction, reactive oxygen species (ROS) generation, and senescence evidence. The cell viability, cell cycle profile, apoptosis induction, intracellular ROS level, and senescence induction were determined in vitro using trypan blue exclusion, propidium iodide (PI) staining, Annexin-PI staining, dichlorofluorescein diacetate staining, and senescence-associated-β-gal method. CCA-1.1 showed cytotoxic activity on 4T1 cells, giving half maximal inhibitory concentration value of 3M, but was less toxic on non-cancerous 3T3-L1 cells. CCA-1.1 induced rapid cell death and inhibited cell cycle progression at the mitotic phase. Instead, of causing apoptosis, CCA-1.1 induced mitotic catastrophe. Furthermore, CCA-1.1 itself increased the intracellular ROS level and induced senescence, possibly through catastrophic cell death. Altogether, our preliminary study strengthens the potency of CCA-1.1 for its anticancer activities against TNBC cells and prospective to be pharmaceutically developed as a novel candidate for cancer therapy.
The aim of the present study was to evaluate the decolourizing and degradation activity of isolated Pseudomonas sp. on Direct Orange 102 dye. The said bacterial spp. was isolated from soil of dumping area of Nagpur (MH) India. Bacterial decolourization and screening of decolorizing bacteria were done by using dye. Colorless colonies was obtained and then it's characterization including morphological characteristics, biochemical characterization was carried out and from biochemical analysis bacteria found to be Pseudomonas sp. In further study, effect of physiochemical parameter such as temperature, pH, concentration, carbon source, nitrogen source and potassium source on decolourization of Direct Orange 102 dye at wavelength 480 nm was carried out. The analysis on dye degraded metabolite was done with TLC show difference in the Rf value confirms the degradation of dye. The isolated bacteria were co-immobilized in Ca-alginate beads and its decolourization efficiency was determined and found to be increase with increase in time.
IL-10 serum level in thalassemia patients infected and non-infected with HCV and control
Thalassemia is the most common congenital hemolytic anemia due to partial or complete lack of synthesis of _-globin chains caused by mutations that affect the synthesis this-chains. The aim of this study is the detection of IL-10-1082A\G polymorphism and IL-10 serum level with it correlation to progression of disease. Case-control study was performed to 60 patients with β-TM diagnosed at thalassemia-center in AL-Zahra hospital in AL-Najaf city, Iraq with group of 40 healthy individual was used as control, the patients were ( 26 male and 34 female) at age 3–49 from which 18 patients infected with Hepatitis C virus. Blood sample was collected from all patients and control. Blood used for DNA extracted for using SSP-PCR in detection the IL-10-1082A\G polymorphism. IL-10 level were measured by enzyme-linked immunosorbent assay test(ELISA). The result shown that male (56.6%) more than female (42.6%), and the age range (10-19) were highest than other age range. This result explain that HCV infected patients less than non-infected thalassemia patients and the infected male more than female, the age group (10-19) was more infected with HCV. The result demonstrate that AA genotype and A allele is risk factor of severity in thalassemia patients, while GG genotype and G allele is protective factor for severity. The result explain that GG genotype is risk factor for HCV infection. This result also shown that IL-10 level is significantly increase in thalassemia patients than control, also significantly increase in IL-10 level in thalassemia with HCV infection than other patients with no HCV infection and control. Conclusion: The polymorphism in IL-10 at position (1082A\G) has association with progression of thalassemia at AA genotype, and IL-10 consider as predictive factor for severity of thalassemia.
Polyhydroxyalkalonates (PHAs) have numerous industrial and medical applications, is being used in various medical applications such as scaffold, suture, heart valve and drug delivery etc. Poly 3-hydroxy butyrate (PHB) is the most common and important family member of PHAs. The bacterial strains which are able to produce higher quantities of PHA using low-cost substrates are always in demand. In the present study, a local strain of Bacillus sp. SM11 isolated from soil was screened for ability to produce biopolymer i.e., PHB (polyhydroxybutyrate). Various factors which have been found to have an impact on PHB production by the selected capable bacterial isolates were optimized viz., organic waste source (soya extract, whey, molasses, corn extract, and distillery waste liquor), nitrogen source (peptone, beef extract, yeast extract, ammonium chloride, and ammonium sulphate), pH, and trace elements. An optimized PHB yield of 3.53g/L was obtained using whey as a source of carbon, added with 1% of yeast extract as a nitrogen source at pH 6.0 in presence of calcium as a trace element. The outcome of the present study indicates that isolate is among one of the high PHB producing microorganism, using whey as a carbon source.
This study was conducted at the laboratory of department of biology, faculty of science/university of Kufa during the period extended from 7,September, 2016 to 5,February, 2017, 45 male rats that was used which is divided in to three groups, Group 1: male rats administered with drinking water as negative control (N. C.). Group 2: male rats administered with CCL4 for one month, Group 3: male rats undergo Bile duct ligation for one week,. At the end of treatment period, which has been extended for five weeks, male rats have been sacrificed and blood samples obtained for assessment of (P111NP and levels of WBC, Hb and PCV).The result have a significant elevate (p< 0.05) in level of P111NP and WBC in rats undergo BDL and CCL4 treatment compare to control groups.a significant decrease (p< 0.05) in level of Hb and PCV in rats undergo BDL and CCL4 treatment compare to control groups.
Comparison of substance abuse.
Substance abuse among different age groups (c 2 =12.7, df=6, P<.049 for comparison of ages grouped 10-20, 21-40, 41-70 years and alcohol, amphetamine, cannabis, and polysubstances).
Sociodemographic distribution of substance abusers (n=612).
Level of education among substance abusers.
Association of positive family history of substance abuse and mental illness among abusers (n=612).
Background: Lornoxicam is an important non-steroidal anti-inflammatory agent. It is practically insoluble in water leading to difficulty in formulation and adversely affecting its pharmacokinetic and pharmacodynamic properties. For drugs like lornoxicam, the rate of absorption is often controlled by the rate of dissolution. Thus the enhancement of the dissolution rate and in turn solubility of poorly soluble drugs is important. Aims: The purpose of this present study was to prepare binary and ternary systems with lornoxicam, carrier and surfactant. Materials and Methods: Binary solid dispersions of lornoxicam with Gelucire 50/13were prepared at different drug to carrier ratios(1:1), (1:3), and (1:5). Polysorbate 80, a nonionic surfactant, was incorporated as a third component to obtain the ternary solid dispersion system. The solubilizing and absorption enhancer properties of this ternary solid dispersion system was then investigated. Solid systems were prepared by mixing or co precipitation and were characterized by different scanning calorimetery, X- ray diffractometery followed by tests for dissolution behavior. Results: The results thus obtained showed a remarkably improved dissolution of drug from the ternary solid dispersion systems when compared with the binary solid dispersion systems. In order to verify the improved dissolution therapeutically, a paw edema test for the formulation was carried in rats where in the ternary system exhibited a potent local anti-inflammatory activity against carrageenan induced paw edema when compared to pure drug. Thus vindicating the solubilizing and dissolution enhancing effect due to the addition of third additive to the binary system of lornoxicam. Conclusions: The drug loaded lornoxicam binary solid dispersion with GL50/13 exhibited faster dissolution rate than drug alone. The significant high drugdissolution rate was obtained in a ternary solid dispersion system using tween 80 as a third component. The fastest drug dissolution obtained was in the ratio of 1:5:1 w/w/w (LOR /GL50/13/ tween 80). The in vivo experimental results corroborated the in vitro outcomes of lornoxicam.
The current therapy for patients with thyroid cancer is total or near to total thyroidectomy with Radio Iodine treatment. Subsequent to the thyroidectomy the patients are on Levothyroxine as a supplement therapy to maintain the Thyroid hormone levels.Levothyroxine has been considered as a narrow therapeutic index drug. In Thyroid cancer patients, radio-iodine ablation procedure causes hypothyroid state which will be further elongated by the administration of sub optimal doses and hyperthyroid symptoms with higher doses. The current study was conducted to evaluate the covariates influencing the drug concentration of levothyroxine in our target population, i.e Patient with Papillary carcinoma, undergone total thyroidectomy followed by¹³¹I ablation and on Levothyroxine.
The increased lipid profile is the greatest risk factor for atherosclerosis and other associated complications.Currently, available allopathy medicine has related to the number of side effects. Medicinal plants for hyperlipidemia has no side effects and is relatively inexpensive and locally available. The antihyperlipidemic activity of ethanolic extract of leaves of Alternanthera ficoidea Linn against Triton wr-1339 induced hyperlipidemia in rats. Alternanthera ficoidea Linn administered at a dose of 200mg/kg (p.o) to the triton induced hyperlipidemic rats. Alternanthera ficoidea Linn shows a significant decrease lipid profile levels like serum cholesterol, phospholipids, triglyceride, LDL, VLDL and the significant increase in the level of serum HDL level.
We investigated the antihyperlipidemic activity of Strychnos potatorum seeds. The current study was undertaken to assess the hypolipidemic, hypocholesterolemic and hypotriglyceridemic potential of the seeds using triton WR-1339 (Tyloxapol) induced hyperlipidemia in rats. The animals were divided into five groups: control, hyperlipidemic, hyperlipidemic plus Atorvastatin, hyperlipidemic plus Strychnos potatorum seed powder 100mg/kg and Strychnos potatorum seed powder 200mg/kg. Hyperlipidemia was induced by single intraperitoneal injection of triton WR 1339 at a dose of 400mg/kg. Intragastric administration of seed powder significantly (p<0.05) decreased serum total cholesterol, triglycerides, low density lipoprotein cholesterol, very low density lipoprotein cholesterol levels in rats in dose dependent and time dependent manner compared to hyperlipidemic group. Thus, the findings of the investigation suggest that Strychnos potatorum seeds exhibited quite competitive potential when compared with reference drug Atorvastatin against experimentally induced hyperlipidemia.
Dunaliella tertiolecta is a marine microalgae rich in pharmacologically important xanthophyll carotenoid, zeaxanthin. This study demonstrated ultrasound assisted extraction (UAE) of zeaxanthin from D. tertiolecta (NIOT 141). Initially, the solvent type and antioxidant concentration for zeaxanthin extraction were optimized using one-variable-at-a-time (OVAT) experiments. Subsequently, three factors influencing the UAE yield of zeaxanthin like sonication time, sonication temperature and solid (algal biomass): liquid (solvent) ratio were investigated using response surface methodology (RSM). Both identification and quantification of zeaxanthin was done with aid of RP-HPLC (Reverse phase high pressure liquid chromatography). The data obtained from the experimental runs were fitted into a quadratic model. The model for maximizing the zeaxanthin extraction yield from D. tertiolecta had a high coefficient of determination of 0.9801. The optimized parameters for UAE of zeaxanthin from D. tertiolecta were sonication time of 33.6 min, sonication temperature of 59.2oC with a solid –liquid ratio 1:62 (g ml⁻¹) and a predicted zeaxanthin content of 13.42 mg g⁻¹. The optimized UAE process parameters were verified by triplicate validation experiments and a zeaxanthin content of 13.85 ± 0.15 mg g⁻¹ was obtained. This further confirmed the reliability and precision of the RSM design. The zeaxanthin yield obtained in the optimized UAE of zeaxanthin content was 2.2 fold higher than the zeaxanthin content obtained by conventional solvent extraction process (6.24 ± 0.05 mg g⁻¹). Zeaxanthin from D. tertiolecta was purified and characterized using UHPLC-MS. The results indicated that D. tertiolecta can be used as a potential source of zeaxanthin.
L-asparaginase is an important chemotherapeutic agent used for the treatment of a variety of lymphoproliferative disorders, lymphomas and acute lymphoblastic leukemia. In recent years, the use of L-asparaginase in the treatment of leukemia and other lymphoproliferative disorders has expanded immensely. In view of the potential applications of L-Asparaginase and the need for development of economical methods for improved enzyme production with an overall aim of reducing the cost of the industrial process, solid state fermentation (SSF) can serve as an excellent alternative for increasing enzyme yields. Hence present investigation was aimed at exploring the synthetic ability of L-Asparaginase from Pectobacterium carotovorum (MTCC 1428) and Bacillus circulans (MTCC 490) by providing suitable synthetic mediums.
Correlation between serum estradiol and serum CD147 levels of CHD in menopausal women. 
In the current study, Seventy menopausal women have CHD were admitted to the Coronary Care Unit of AL-Sadder Teaching Hospital in AL-najaf AL-Ashraf during the period from January to April/2016 for the ages were ranging from 40 to 69 years old. The samples were divided into three groups (SA 25, UA 22, AMI 23), while the healthy group was composed of 20.The current study indicated a significant increase(p<0.05) in serum CD147 of CHD compared with healthy group, while a significant decrease (P<0.05)in serum E2 concentration of CHD compared with healthy group. The results also revealed a significant increase (p>0.05)of serum CD147concentration in AMI group as compare with UA group and SA group of CHD, While serum of E2 concentration showed a significantly decrease (p<0.05)in AMI compare with UA and SA groups of CHD. The result showed also a negative correlation between E2 and CD147.
Top-cited authors
Imad Hadi Hameed
  • Al-Qasim Green University
Krishnendu Acharya
  • University of Calcutta
Brundha Mp
  • Madha Medical College
Somanjana Khatua
  • Krishnagar Government College