Multidisciplinary Cancer Investigation

Published by Definitive International Institute of Life Science (DIILI Publication)
Print ISSN: 2476-4922
Introduction: Breast cancer is the most common cause of cancer-related death among women. Recently, extremely low-frequency electromagnetic field (ELF-EMF) has been proposed as a new interfering agent with future therapeutic potentials. Many studies have revealed that cellular processes such as apoptosis in breast cancer are affected by ELF-EMFs. However, more researches are needed to clarify the underlying mechanism of action for these fields. In this study, the apoptotic effect of ELF-EMF on the MC4L2 cell line was examined and the mRNA expression level of the P21 and P38 genes were further investigated. Methods: A triple-positive mouse breast cancer cell line (MC4L2) was purchased from the Genetic Resource Center (Iran). This study was performed on two groups of ELF-EMF exposure (100mT/1 Hz for 5 days, 120 min each day) and sham exposure. Cell viability and apoptosis rate of both the exposure and sham exposure groups weredetermined by flow cytometry. Alterations in the P21 and P38 mRNAs expression levelswere investigated; using real-time PCR. Results: ELF-EMF exposure induced 30% apoptosis in MC4L2 cells compared with the control group. The mRNA expression level of P38 and P21 was significantly increased after ELF-EMF exposure compared to the control group. Conclusions: ELF-EMF induces apoptosis in the MC4L2 triple-positive cell line. Furthermore, this exposure affects important gene expression involved in the cell cycle. Our data propose that ELF-EMF in a specific time, intensity, and frequency could be beneficial for breast cancer treatment. However, more studies are required to confirm our findings.
The Trend of Total and New Visits From 2018 to 2020
The Number of Total and New Visits From 2018 to 2020
The COVID-19 pandemic has greatly changed the routine medical practice in any field and the care delivery to lymphedema patients is not an exception. The lymphedema clinic at Motamed Cancer Institute (MCI), Tehran primarily was established for the management of breast cancer- related lymphedema (BCRL). However, it is now a referral center for different types of lymphedema from all over Iran. This letter aimed to show the impact of the coronavirus disease 2019 (COVID-19) pandemic on the lymphedema practice in the MCI lymphedema clinic.
In late December, when the world faced a huge challenge called Corona New Virus-2019 (SARS- CoV-2), health systems faced lots of challenges. We are in Midway through or perhaps at the beginning of the COVID-19 pandemic. Infection with COVID-19 has completely disrupted nearly every aspect of daily life and is the focus of attention of almost all visual and written media. By the way, cardiovascular and cancer have been identified as risk factors for suffering from COVID-19 and death [1]. Therefore, it is necessary to reduce the risk of COVID-19 as a preventive strategy. Besides, regarding health issues, staying at home is the best way to reduce the risk of being affected by COVID-19. On the other hand, patients with cancer and heart problems require regular supervision and visits. This causes conflict and anxiety for patients and makes them feel confused. Therefore, the best way to solve this conflict and prevent being affected with COVID-19; while continuing the monitoring and therapeutic adherence is using E-visits and E-service. According to the findings of Mohammadzadeh in 2019 using the E-visit was a good way to monitor the pharmacy and prevention of SARS-CoV-2 infection [2]. Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome (SARS-CoV-2) with droplets and contact as the main means of transmission. Although most patients have mild symptoms and good prognosis after infection, some patients with cancer and heart diseases have developed severe and died from multiple organ complications. Social distancing is the best way for the prevention of COVID-19, therefore to support the social distance and continuing therapeutic adherence, the use of cyberspace and E-visit are recommended.
Introduction: The diagnosis of cancer involves the patients, their caregivers, and families. Caregivers should obtain guidance from health care professionals to promote care facilities. The current study aimed at investigating the economic, socio-individual, informational, and psycho-spiritual problems of caregivers in post-treatment phase of breast cancer. Methods: The present descriptive study was conducted on 251 subjects using a ques- tionnaire consisted of demographic part and the caregivers’ difficulties. Answers were estimated based on a Likert scale. Data were analyzed using SPSS software. Results: Totally, 42% and 37% of participants were spouses and close relatives, re- spectively; 97%, 89%, and 74% of caregivers had psychological, economic, and spiri- tual problems, respectively. All of the caregivers had individual, emotional, physical, economic, and informational problems with care giving (P<0.0001). Significant rela- tionships were observed between the employee group and individual, emotional, infor- mational, and economic problems (P<0.0001). There were significant negative relation- ships between either of the occupational, educational, relationship, caregiving duration, and marital status with spiritual problems (P<0.0001). Significant correlation was ob- served between the duration of caring and the socio-individual, physical, informational and economic problems (P<0.0001). Conclusions: Although the current study reported insignificant relationships between the socio-individual, informational, and psycho-spiritual problems of caregivers and some demographic variables, related positive trends were observed. Future studies with powerful strategies are needed to alleviate the observed concerns.
Different Mechanics of Lumen Formation.
Contact of Tip Cells A) Green and purple cells connect. Yellow shows cell connection; B) Cell junction area increases; C) New junction surface and apical membrane (dark green and dark purple) form
Formation of the Multicellular Lumen During Anastomosis A and B) formation of the new junction; C) The detachment of the middle cell; D) Formation of the multicellular lumen
Formation of the Unicellular Lumen During Anastomosis A and B) Invagination of the green cell's membrane into the purple cell; C) The fusion of the green cell membrane with its membrane; D) Invagination of purple's apical membrane (neighboring cell); E) Formation of the unicellular lumen Membrane invagination is the second mechanism which causes unicellular lumen structure. Figure 4 shows this mechanism. When cell junction and
Inverse Membrane Blebbing Mechanism A) Membrane local weakening because of blood pressure; B) Bleb formation and lumen expansion; C) F-actin polymerization on the bleb membrane; D) Bleb retraction: Myosin II recruitment, the formation of actomyosin fiber and its contraction
Different mechanisms such as cell migration, proliferation, branching, anastomosis, and lumen formation occur during the angiogenesis process. Lumen formation is one of the critical mechanisms which is not only necessary for the functional plexus but also for continuing of angiogenesis process. Although multiple studies investigated this mechanism during the angiogenesis process in both in vivo and in vitro conditions, it is not fully understood yet. Different studies have suggested distinctive mechanisms as the main mechanism for lumen formation. Recent studies, however, have shown the fundamental role of blood flow hemodynamics, especially during in vivo lumen formation. This newly introduced mechanism is called ''inverse membrane blebbing''; suggesting that blood pressure causes the formation and expansion of lumen during the in vivo angiogenesis process. This paper reviews cell behavior during lumen formation in the angiogenesis process on a cellular scale.
Morphologic Changes in MCF-7 and HU02 Cells Treated with Essential Oils with a Dose of 100µg/mL of Red Clover and K. scoparia. a: MCF-7 control cells; b: HU02 cells; c-e: MCF-7 cells treated with red clover essential oils; f-h: MCF-7 cells treated with K. scoparia essential oils; i-k: MCF-7 cells treated with red clover essential oils, l-n: MCF-7 cells treated with broom grass essential oils within 24, 48, and 72 hours. All the pictures have a 50-micrometer scale bar. 
Test Results of One of the Triplicate Mean Cell Viability Tests for MCF-7 Cells After Incubation with Red Clover's Essential Oil as Mean ± SD
Test Results of one of the Triplicate Mean Cell Viability Tests for MCF-7 Cells After Incubation with Essential Oil of K. scoparia as Mean ± SD
Introduction: Breast cancer is one of the most common malignancies among Iranian women. Nowadays, the use of traditional medicinal plants has emerged as a tempting complementary to the treatment of breast cancer due to minimal side effects and less documented drug resistance. The aim of this study was to examine the anticancer effects of the essential oils of red clover and mature fruit of Bassia scoparia on MCF-7 cells. Methods: Essential oils were extracted from Bassia scoparia and red clover plants, by a Clevenger condenser and mass spectrometry was performed for qualitative analysis, then MCF-7 and HU02 cells were treated with different concentrations of these essential oils at different time intervals. The viability of cells was measured by the MTT assay. The results were analyzed using the one-way ANOVA and Tukey′s test. Results: Essential oils of Kochia scoparia and red clover have cytotoxic effects on MCF7 cells. The MTT analysis showed decreased cell viability percentage in treated cells (P < 0.05). In addition, the microscopic examination of cells treated with essential oils revealed morphological changes of apoptotic cells in MCF-7 cells. Conclusions: The findings of this study show that essential oils of Bassia scoparia and Red clover have toxic and anticancer effects on MCF-7 cells.
Introduction: This study aimed to examine the effectiveness of group-based compassion-focused therapy (CFT) on depression, anxiety, and improving the quality of life (QoL) in women with feminine cancers. Methods: A pretest-posttest control‐grouped, quasi‐experimental study was carried out on all women with cancer who were referred to the Omid Hospital of Mashhad in 2019. Thirty patients with various types of feminine cancers were randomly assigned to 12 weeks of group-based compassion-focused therapy (CFT) (n=15) or sham control (n=15). Participants in both conditions completed measures of Beck Depression Inventory-II (BDI-II), Beck Anxiety Inventory (BAI-II), and the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30) at pretest and posttest. Analysis of covariance was used for data analysis. Results: Our results showed that there was a significant difference between experimental and sham control groups in depression (F= 4/499, P<0.05, d=1.60 large) and anxiety (F=19/99, P<0.01, d=1.96 large), but there was no significant difference in participants’ QoL scale. Conclusions: Group-based CFT could be considered a promising and potentially useful intervention to alleviate the depression and anxiety in cancer patients but it did not affect their QoL. Nevertheless, future randomized trials are needed.
Top-cited authors
Akram Sajadian
  • Motamed Cancer Institute,Iran,Tehran
Shahpar Haghighat
  • Breast cancer Research Center, ACECR
Majid Akrami
  • Shiraz University of Medical Sciences
Vahid Zangouri
  • Shiraz University of Medical Sciences
Sedigheh Tahmasebi
  • Shiraz University of Medical Sciences