Frontiers in Veterinary Science

Phages play an important role in shaping the gut microbiome. CrAss-like phages, which are key members of the gut virome, show high abundance in the human gut and have attracted increasing interest. However, few studies have been found in pigs, and the distribution of crAss-like phages across broader pig populations remains unknown. Here, we obtained 1,251 pig crAss-like phage genomes from 403 metagenomes publicly available and a pig gut virome dataset constructed by ourselves. These crAss-like phage genomes were further clustered into 533 virus operational taxonomic units (vOTUs). Phylogenetic analysis revealed that crAss-like phages in pig guts were distributed across four well-known family-level clusters (Alpha, Beta, Zeta, and Delta) but were absent in the Gamma and Epsilon clusters. Genomic structure analysis identified 149 pig crAss-like phage vOTUs that utilize alternative genetic codes. Gene blocks encoding replication and assembly proteins varied across crAss-like phage clusters. Approximately 64.73% of crAss-like phage genes lacked functional annotations, highlighting a gap in understanding their functional potential. Numerous anti-CRISPR protein genes were identified in crAss-like phage genomes, and CAZymes encoded by these phages were primarily lysozymes. Host prediction indicated that bacterial hosts of pig crAss-like phages primarily belonged to Prevotella , Parabacteroides , and UBA4372 . We observed that interactions between crAss-like phages and Prevotella copri might have a possible effect on fat deposition in pigs. Finally, all detected vOTUs exhibited low prevalence across pig populations, suggesting heterogeneity in crAss-like phage compositions. This study provides key resources and novel insights for investigating crAss-like phage-bacteria interactions and benefits research on the effects of crAss-like phages on pig health and production traits.

Objectives To identify barriers to veterinarian-producer partnerships and suggest collaborative applied education as a means to enhance economic efficiency and sustainability of small and medium livestock operations and rural veterinary practices. Materials and methods A participatory needs assessment, exploring the willingness and barriers to producer-veterinarian partnerships to enhance small/medium livestock operations, was distributed to Texas producers and veterinarians. Quantitative and qualitative data were collected via online, closed-ended survey questions and free response interviews. Responses were analyzed using SPSS and HyperRESEARCH to identify relevant terms, ideas, patterns, or themes. Results Similar responses from 115 veterinarians and 58 producers revealed five major themes regarding relationship barriers: time, financial challenges, communication, competing perspectives, and respect. Overall producers reported greater willingness to partner in all areas, health care (90%), to achieve goals (80%), and to expand business (70%), than veterinarians. Veterinarian interviews revealed a need for increased animal health education among producers, while more than 60% of producers expressed high interest in continuing education on animal health topics. Discussion Veterinarians and producers experience similar barriers to establishing partnerships. Both groups also recognize a need for education and prefer in-person collaborative learning communities Such educational opportunities can encourage formal veterinary-producer partnerships and provide solutions that enhance the economic efficiency and sustainability of small/medium livestock operations.

The Mediterranean pond turtle ( Mauremys leprosa ) is increasingly recognized as a sentinel species for freshwater ecosystem health due to its resilience to environmental pollutants. Despite its ecological significance, there is a lack of research on the blood profile of this vulnerable species. This study assessed the hematological and biochemical profile of healthy individuals admitted to Zoomarine ’s Porto d’Abrigo rehabilitation center in southern Portugal between 2015 and 2024. This investigation revealed significant differences between the established data and the reference intervals reported in a Spanish study, notably in total erythrocyte (RBC) counts, packed cell volume (PCV), differential counts of lymphocytes and eosinophils, total protein (TP), creatine kinase (CK), potassium (K ⁺ ), phosphorous (PHOS), and glucose (GLU) levels. Significant differences were also observed between captive and wild-rescued individuals in monocyte percentages, aspartate aminotransferase (AST), and CK levels. No seasonal influence was detected except in the differential count of eosinophils. These findings indicate a possible geographical, environmental, and captivity-induced variation, providing the first comprehensive species-specific hematological and biochemical intervals for M. leprosa derived from a healthy population. This study enhances the understanding of M. leprosa health within a One Health framework by offering critical diagnostic benchmarks for veterinary care and facilitating conservation strategies.

Acute mesenteric ischemia (AMI) refers to a group of vascular disorders that disrupt intestinal blood flow, resulting in intestinal ischemia and necrosis if left untreated. In both humans and dogs, this condition appears to be rare but deadly. There has only been one documented case of traumatic acute mesenteric ischemia in a dog, and the diagnosis was conducted through laparotomy. We present the case of a dog that was struck by a vehicle and subsequently developed traumatic acute mesenteric ischemia, which was diagnosed through computed tomography (CT). The dog presented with profuse hemorrhagic diarrhea, which persisted throughout the hospitalization. The dog’s condition eventually deteriorated as a result of diffuse intestinal ischemia, diagnosed using computed tomography (CT). Due to the anticipated poor prognosis, the dog was euthanized humanely. Traumatic acute mesenteric ischemia in dogs may be an underdiagnosed consequence of vehicular trauma, necessitating further diagnostic investigation in dogs with persistent gastrointestinal signs that are unresponsive to medical therapy.

Introduction In a 10–15-year period, veterinary clinics in Sweden and Norway, as elsewhere, have undergone widespread corporatisation. High veterinary care costs have received attention in the lay press and from competition authorities. Whether corporate chains and independent clinics differ in price levels and how clinic characteristics, such as on-call service, affect pricing is not well-documented. The aim was to analyse prices levels and price changes for various diagnoses/procedures for dogs, cats, and horses from clinics in Norway and Sweden and to examine the influence of affiliation (corporate chain, government-run, or independent), extraction date, and clinic characteristics (e.g., on-call service) on prices. Materials and methods Data from a price comparison site were extracted five times between 2 January 2023 and 2 January 2024. Prices for procedures such as vaccinations, gonadectomy, euthanasia, emergency care, diagnostic imaging, certification, and planned surgery were included. Descriptive statistics and mixed models were used to analyse effects of affiliation (Anicura, The Swedish District Vet Officers (DV), Dyrenes venn, Empet, Evidensia, Vettris, and independent), clinic characteristics (animal hospital or not, on-call service, and number of hours open Mon-Fri), and extraction date. Results Prices were analysed for 37 procedures (16 dogs, 11cats, and 10 horses) from 771 clinics, of which 502 (65%) were independent. Most clinics with corporate affiliation belonged to Evidensia and Anicura. In statistically significant comparisons, their prices were generally higher than those from the independent group. For Anicura, the median annual price increase (in Euro) was 8%, DV 5%, Dyrenes venn 53%, Empet 12%, Evidensia 15%, Vettris 7%, and the independent group 6%. Multivariable results generally corroborated the descriptive figures. Discussion Targeting a range of procedures in two nearby countries, veterinary care prices varied with country, clinic characteristics, and affiliation. Clinics belonging to corporate chains charged higher prices than independent clinics. Most prices increased over the year. Possible reasons for the differences between clinics are investments in equipment or number of staff, expenditure on continued education of staff, or different demands for profit. Increased price transparency within veterinary care might reduce the impact of high prices and perhaps also limit price increases.

In temperate regions, boars experience environmental heat stress due to the subtropical climate, leading to their semen quality and fertility being severely compromised compared to indigenous breeds. Considering the above effect, the present study aimed to evaluate the effect of season on semen quality, seminal plasma antioxidant status, and in vivo fertility of crossbred boars of exotic (50%) and indigenous inheritance in a subtropical climate. A total of 14 Hampshire–Ghungroo crossbred boars were used for this investigation, which took place in both summer and winter. Sperm characteristics, namely motility, viability, abnormality, acrosomal integrity, and the hypo-osmotic swelling test (HOST) results, and mitochondrial membrane potential (MMP) were evaluated. Sperm kinematics parameters were assessed using computer-assisted semen analysis (CASA). Antioxidant biomarkers (glutathione peroxidase, GPx; catalase, CAT; and total antioxidant capacity, TAC) and lipid peroxidation (malondialdehyde, MDA) were analyzed in boars’ seminal plasma. The summer season had a significant ( p < 0.01) negative impact on reaction time and false mounts, whereas semen volume and sperm concentration were significantly ( p < 0.01) higher in the winter season. Similarly, sperm abnormalities were significantly ( p < 0.01) lower in the winter season. In the winter, sperm quality parameters, namely total motility, progressive motility, viability, acrosomal integrity, and HOST reactivity, were significantly ( p < 0.01) improved. However, during the summer, sperm MMP was significantly ( p < 0.01) lower in fresh samples and after 72 h of storage. Season had a significant ( p < 0.05) effect on the following sperm kinematics parameters: average path velocity, straight-line velocity, curve linear velocity, amplitude of lateral head displacement, and beat cross frequency. Semen characteristics were significantly ( p < 0.01) improved in winter after 72 h of cold storage compared with those in summer. The summer season had a significant effect ( p < 0.01) on seminal plasma antioxidant biomarkers (TAC, MDA, CAT, and GPx). Furthermore, the farrowing rate was significantly ( p < 0.05) higher in the winter season. In conclusion, our results showed that the low MMP of boar sperm and the downregulation of seminal plasma antioxidant biomarkers in summer lead to poor semen quality and poor fertility in Hampshire–Ghungroo crossbred boars in a subtropical climate. To alleviate the heat-stress-induced poor sperm fertility in boars and to optimize the fertility of boars during summer in subtropics, there is a need for scientific interventions in terms of genetics [less exotic inheritance (below 50%)], nutrition, and management.

The objective of this study was to investigate the effects of fermented wheat bran (FWB) on growth performance, nutrient digestibility, serum biochemistry, short-chain fatty acids, and intestinal microbiota of weaned piglets. One hundred twenty-eight weaned piglets were randomly assigned to 4 groups, each with 8 pens and 4 piglets per pen: basal diet group (BD), 5% wheat bran group (5% WB), 5% fermented wheat bran group (5% FWB), and 10% fermented wheat bran group (10% FWB) for a 28-day trial. Results showed that compared to the BD group, the diarrhea rate in the 5% WB group was significantly increased ( p < 0.05) at d 15–28 and d 1–28. In contrast, at d 15–28 and d 1–28, the diarrhea rates in the 5% FWB and 10% FWB groups were significantly lower than those in the 5% WB group and showed no significant difference compared to the BD group. Moreover, the apparent total tract digestibility (ATTD) of DM, GE, CP, EE, CF and ADF at d 1–14, and EE and NDF at d 15–28 in the 5% FWB group were significantly improved compared to the 5% WB group ( p < 0.05). However, only the ATTD of CP, EE and CF at d 1–14 in the 10% FWB group were significantly higher than those in the 5% WB group ( p < 0.01). Compared to the BD group, the pH of cecum chyme and serum urea nitrogen content in the 5% FWB and 10% FWB groups were significantly reduced ( p < 0.05), and those in the 10% FWB group were significantly lower than those in the 5% WB group ( p < 0.01). The propionic acid content of cecum chyme in the 5% FWB and 10% FWB groups, and butyric acid content in the 10% FWB group were significantly higher than those in the BD group ( p < 0.05). LEfSe analysis (LDA score > 3.0) identified 4 species, 6 species of Proteobacteria, 2 species, and 9 species that were enriched in the BD, 5% WB, 5%F WB and 10%F WB groups, respectively. Additionally, Dialister , Prevotellaceae_NK3B31_group , Mitsuokella , Succinivibrio , and Prevotella were significantly and positively correlated with the concentrations of valeric acid, propionic acid, and acetic acid ( p < 0.05). In conclusion, 10% FWB supplementation in weaned piglet diets did not affect growth performance, it reduced the diarrhea rate compared to the 5% WB group, potentially due to enhanced nutrient digestibility, elevated SCFAs levels, and shifts in microbial composition.

Introduction Erigeron breviscapus exhibits anti-inflammatory properties, protects neuronal cells and enhances immune function. Modern traditional Chinese medicine fermentation techniques can increase the bioactive compound content in Erigeron breviscapus . However, its potential therapeutic effects against the porcine pseudorabies virus (PRV) remain unclear. Methods A PRV infection model was established in mouse trigeminal ganglion (TG) cells to determine the optimal antiviral mode of action of flavonoids from fermented Erigeron breviscapus (FEBF). Additionally, a PRV-infected rat model was developed to evaluate the in vivo antiviral efficacy of FEBF. Results FEBF demonstrated a higher protective rate and a lower viral copy number compared to unfermented E. breviscapus flavonoids (EBF). The protective effect was most pronounced under toxicological and inhibitory conditions, surpassing the blocking effect. PRV infection upregulated TLR4, MyD88, and NF-κB p65 protein expression during the pre-infection phase, followed by their downregulation after 12 h. FEBF regulated PRV-induced changes in protein expression, restoring them to near-normal levels by 36 h. In vivo assessments of pathological injury, PRV viral load, neuronal count, and neuronal apoptosis indicated that FEBF provided superior neuroprotection compared to both Minocycline (MINO), a broad-spectrum neuroprotective drug, and unfermented EBF. Mechanistic studies further revealed that FEBF modulated microglial polarization and regulated the inflammatory cytokines IL-6, TNF- α , IL-4, and IL-10. Conclusion These findings demonstrate that FEBF exhibits significant antiviral effects against PRV in both in vitro and in vivo models. FEBF represents a promising candidate for the development of anti-PRV therapeutics.

This study evaluates the impact of dietary supplementation with hemp-based polyunsaturated fatty acids (PUFAs) on the membrane lipid profiles and reproductive performance of Martina Franca jacks. Over a 90-day period, twelve donkeys were divided into a treatment group receiving hemp oil and a control group on a standard diet. Semen and blood samples were collected and analyzed at multiple time points for lipid composition and reproductive parameters. Results revealed that sperm motility improved significantly, increasing from 92.61% in the control group to 96.63% by Day 60 in the treatment group ( p = 0.05). Normal sperm morphology also showed a significant enhancement, rising from 96.58% in the control group to 98.85% by Day 60 ( p = 0.04). Conversely, gel-free semen volume decreased significantly in the treatment group, from 64.17 mL in the control group to 28.20 mL at Day 60 ( p < 0.0001). Lipidomic analyses indicated an increased proportion of omega-3 PUFAs, such as eicosapentaenoic acid (EPA; 0.08% in the control group to 0.20% by Day 60, p < 0.0001), in sperm membranes, alongside a reduction in the peroxidation index (264.11 in the control group to 86.53 in the treatment group, p < 0.0001). These changes suggest improved membrane fluidity and oxidative stability. These findings underscore the potential of hemp oil as a dietary supplement to enhance reproductive outcomes in donkeys, with broader implications for livestock management.

Introduction The prevalence of lipid metabolism disorders, including obesity, increases with age in cats and humans. Obesity is a condition characterized by systemic low-grade inflammation and oxidative stress caused by excessive visceral fat accumulation. Resveratrol (RSV), a natural plant polyphenol, modulates the expression of anti-inflammatory factors. This study aimed to investigate the effects of resveratrol supplementation on lipid metabolism in both healthy and obese cats and assess its potential as a dietary supplement for improving lipid metabolism disorders in this population. Methods Plasma metabolite and hormone concentrations, and enzyme activities were measured in healthy, obese, and overweight cats supplemented with RSV for 4 weeks. RVS was supplemented at 1 mg/kg body weight/day (low dose) and 5 mg/kg/day (high dose) in capsules for 4 weeks. Results Body weight, body condition score, BUN, and insulin concentrations did not change in obese or overweight cats with RSV supplementation for 4 weeks. Plasma triglyceride, free fatty acids, and serum amyloid A (SAA) concentrations and lactate dehydrogenase (LDH) activities decreased, and adiponectin concentrations increased markedly in obese and overweight cats after RSV supplementation. Discussion Decreased plasma SAA concentrations and LDH activities and increased plasma adiponectin concentrations in obese and overweight cats seem to be induced by the improvement in liver function and the anti-inflammatory effect of RSV. Moreover, RSV supplementation may be useful in treating lipid metabolism disorders, including obesity, in cats.

Objectives The aim of the study was to analyze glucocorticoid (GC) dosing protocols in dogs with hypoadrenocorticism (HA), and to identify predictors for optimal clinical outcomes during both the acute and chronic phases of the disease, as well as during long-term therapy. Methods This observational cross-sectional study utilized a case-based online questionnaire. Practicing veterinarians across Germany were invited to participate. The survey collected data in the disease course and follow-ups. Responses were analyzed using descriptive statistics, single and multiple comparisons, and a multivariable logistic regression model. Results For 103 dogs the questionnaire was fully completed and analyzed. Of these, 85 dogs (82.5%) were hospitalized, and they received either prednisolone (52.9%), dexamethasone (31.8%) or hydrocortisone (11.8%). Hydrocortisone therapy was associated with a shorter hospitalization time and faster normalization of electrolytes compared to prednisolone. Follow-up data were available for 85 dogs, with 82.35% ( n = 70/85) achieving an optimal or well-adjusted clinical outcome. Multivariable logistic regression analysis indicated that the eukalemic and eunatremic form was significantly less associated with the presence of azotemia and the occurrence of an acute adrenal crisis. Dividing the daily GC dosage was associated with poorer clinical outcomes and a reduced likelihood of achieving optimal medication adjustment. Conclusion Our findings provide new, relevant recommendations for the therapeutic management of HA in dogs. Hydrocortisone appears to be a promising treatment for managing HA during hospitalization, highlighting its potential use in clinical practice. Once-daily administration of prednisolone is advisable for long-term therapy. To achieve the best possible outcome, implementing an optimal treatment protocol is essential, which veterinarians should tailor based on the needs of both owners and animals. The main limitations of the study include its retrospective nature and the limited number of participants. Future studies, particularly prospective ones, could further validate the beneficial effects of hydrocortisone and evaluate long-term therapy in comparison to prednisolone.

Pseudorabies virus (PRV) poses a serious threat to the global swine industry, as PRV infection can lead to reproductive disorders in sows and high mortality in newborn piglets. Although pigs typically exhibit age-related resistance to PRV, with older pigs exhibiting milder symptoms upon infection, the recent isolation of multiple highly pathogenic PRV variants and reports of severe symptoms and even death in older pigs have garnered much attention. The GDWS2 strain isolated in this study exhibits characteristics similar to those of highly pathogenic strains. GDWS2 was isolated from the brain tissue of a 90-day-old diseased pig that exhibited severe respiratory and neurological symptoms. The pig originated from a farm that had been previously vaccinated with the Bartha-k61 strain. In vitro experiments demonstrated that GDWS2 induces substantial cytopathic effects in PK-15, VERO, BHK cells, and PAM. Moreover, GDWS2 formed larger plaques and exhibited higher early replication titers in PK-15 cells compared to the highly pathogenic variant strain JM isolated in China. Phylogenetic analysis revealed that GDWS2 belongs to PRV genotype II, with gD , gE , and TK genes showing high homology to those of highly pathogenic PRV variants. Additionally, GDWS2 harbors unique insertions or mutations in the US1 , UL36 , and UL5 gene regions, and its genome contains recombination events with PRV variants, Bartha, or genotype I strains. In vivo experiments further confirmed the high pathogenicity of GDWS2. In rabbit and 90-day-old pig models, GDWS2, compared with the JM strain, caused high mortality rates, accompanied by severe pathological damage. Notably, in the 90-day-old pig model, the GDWS2 challenge group exhibited more severe respiratory and neurological symptoms, and enhanced neurotropism and shedding capacity. The data from this study may indicate the emergence of a naturally recombined and highly pathogenic PRV variant in China once again.

Along with the increasing litter sizes in pig industry, using milk replacer (MR) as a nutrient supplement has been widely practiced, yet the effects of replacing sow milk (SM) with MR on growth and development of piglets remain unclear. This study evaluated the differential effects of MR versus SM on growth performance, body composition, muscle fiber types, and intestinal health of piglets during the neonatal and nursery periods. Forty 2-day-old piglets, selected from 10 healthy sows, were randomly divided into two groups receiving either SM or MR ad libitum until postnatal day 23 (PND 23), then transitioned to be fed with nursery diet until PND 37. Blood, muscle, and intestinal tissues, along with colonic digesta and carcass samples, were collected on PND 12 ( n = 10) and PND 37 ( n = 10) for analysis of parameters related to intestinal function, microbiota composition and muscular development. The results showed that MR-fed piglets had lower average daily gain (ADG) and higher diarrhea index during the neonatal period. During the nursery period, however, MR-fed piglets had significantly higher average daily feed intake (ADFI) and ADG. Compared to SM-fed piglets, MR-fed piglets had a lower percentage of fast twitch fibers, but a higher percentage of slow twitch fibers on PND 12, along with lower body fat content on both PND 12 and PND 37. In addition, MR-fed piglets had significantly deeper crypt depth, increased mRNA expressions of inflammatory genes, and lower alpha diversity on PND 12. On PND 37, however, MR-fed piglets had higher villus height, increased sucrase activity and alpha diversity. On PND 12, likewise, MR-fed piglets were enriched with Prevotella associated with diarrhea, while SM-fed piglets were enriched with Lachnospiraceae associated with body fat deposition. In contrast, on PND 37, MR-fed piglets were enriched with commonly recognized beneficial bacteria, such as f_Muribaculaceae, g_Prevotellaceae_NK3B31_group , f_Oscillospiraceae and f_Rikenellaceae. These findings indicate that piglets fed MR experienced temporary growth check and intestinal complications in neonatal period, but intriguingly MR piglets had higher feed intake, compensatory growth, and recovery of intestinal function during the nursery period.

This study investigated the effects of a commercial polyphenol blend on broiler performance, meat quality, carcass traits, and the incidence of pectoral myopathies. Broilers (1–42 days old) were allocated to four treatments: T1 (control, basal diet), T2 (250 g/ton polyphenol blend), T3 (500 g/ton), and T4 (1,000 g/ton), with eight replicates of 40 birds each. All diets were corn-soy based, isonutritional, and formulated to meet age-specific nutritional requirements. Parameters assessed at 21, 28, 35, and 42 days included antioxidant potential, growth performance, myopathy incidence, carcass yield, allometric growth, muscle morphometry, meat quality, and lipid profile. Optimal performance was observed at a supplementation level of 514 g/ton of polyphenols. While carcass yield remained unaffected, birds fed 500 g/ton exhibited delayed breast growth relative to other body parts, suggesting modulated allometric growth. Polyphenol supplementation reduced breast muscle fiber size, increased fiber density, and lowered the severity of wooden breast without influencing the incidence of white striping. Improved meat tenderness was evident through reduced cooking weight loss and enhanced shear force. Antioxidant status improved in plasma, muscle, and liver tissues, and the muscle lipid profile was favorably altered. In conclusion, the polyphenol blend enhanced broiler zootechnical performance, alleviated wooden breast severity, and improved meat quality and tenderness.

Deoxynivalenol (DON) and Zearalenone (ZEN), common symbiotic mycotoxins found in mold-contaminated cereal feed, adversely affect broiler’ health. Glycyrrhiza uralensis has various pharmacological effects including antibacterial, antioxidant and immunomodulatory. This study aimed to investigate the effects of the long-term intake of low doses of DON and ZEN on growth performance and intestinal health of broilers, as well as the potential protective effect of supplementary Glycyrrhiza uralensis extract (GUE) in an 84-day feeding experiment. A total of 315 one-day-old male Liangfeng broilers were randomly assigned to three treatments: basal diet (CON), MOL diet (where 5% of corn in the basal diet was replaced with an equal amount of naturally moldy corn) containing DON and ZEN at 1.25 and 1.29 mg/kg, and MGUE diet supplemented with 0.1% GUE in the MOL diet. The MOL diet reduced the body weight (BW) of broilers at 56 and 84 day, body weight gain (BWG) and feed intake (FI) aged 1-56 and 1-84 days, and the feed conversion ratio (FCR) aged 1-84 days, as well as villus height (VH) and the villus/crypt (V/C) ratio, SOD and GSH-Px activities, and the expression of claudin-1 , occludin and ZO-1 , while increasing MDA level, the expression of TNF-α , IL-1β and IFN-γ in the jejunum of broilers. Additionally, MOL diet decreased the Firmicutes to Bacteroidetes (F/B) ratio and abundances of Lactobacillus ( L.gallinarum and L.crispatus ), and B.vulgatus , while increasing Bacteroides ( B.fragilis and B.dore ), Helicobacter ( H.pullorum ), and Escherichia ( E.coli ) in the ceca. In contrast, MGUE diet improved growth performance and returned it to a level comparable to that of the CON diet, increased VH and V/C ratio, SOD and GSH-Px activity, claudin-1 , occludin and ZO-1 expression, while reducing MDA level, the expression of TNF-α , IL-1β and IFN-γ in the jejunum. Moreover, MGUE diet had a greater F/B ratio and abundance of Lactobacillus ( L.gallinarum and L.crispatus ) and B.vulgatus , while reducing Bacteroides ( B.fragilis and B.dorei ), Helicobacter ( H.pullorum ) and Escherichia ( E.coli ) in cecum. In conclusion, the long-term consumption of a low-dose DON-ZEN contaminated diet decreases growth performance and disrupts intestinal health and microbiota balance in broilers; however, dietary supplementation with GUE effectively mitigates the damage caused by DON-ZEN contamination.

The current research evaluated the consequence of varying stocking densities on growth performance, carcass features, hematological, welfare, economic parameters, and immune markers of broiler chicks. A total of 324 Avian 48 were haphazardly classified into three different stocking densities. There were 14 birds/m² in the low stocking density (LSD) group, 18 birds/m² in the medium stocking density (MSD) group, and 22 in the high stocking density (HSD) group. Compared to the other two groups, the HSD birds’ body weight and daily weight gain were significantly lower (p < 0.05). The LSD group demonstrated a significant increase in productive efficiency (EPEF and EBI) compared to the medium and high SD groups (p < 0.003). The birds from the HSD group exhibited the lowest values for carcass characteristics compared to the low and medium SD groups. At the hematological level, the HSD group exhibited significantly elevated levels of HB, RBCs, heterophils, and lymphocytes compared to the LSD and MSD groups (p < 0.011, p < 0.0001, and p < 0.0001), respectively. Compared to the LSD group, the levels of cortisol, a hallmark of oxidative stress, were considerably greater in the MSD and HSD groups (p < 0.0001). Concerning gene expression, the birds in the LSD group exhibited a significant improvement in growth, intestinal health, and anti-inflammatory genes compared to the MSD and HSD groups. In addition, inflammatory markers were significantly downregulated. The HSD group exhibited the lowest net profit compared to the other groups (p < 0.0001). At the behavioral level, birds in the LSD group demonstrated a significantly shorter TI duration (p < 0.0001) and latency (p < 0.043) in OFT to the first step, lower mobility duration (p < 0.004), and pecking (p < 0.05) compared to other groups. Our study concluded that rearing in LSD up to MSD could be applied without compromising broiler performance.

Introduction Teaching and learning methods in Higher Education are constantly adapting looking for a better achievement of one day competencies in each subject along the Veterinary Medicine Degree. Alfonso X el Sabio University is developing its innovative and pedagogical model, entirely centered on the students and based on the development of the most demanded soft skills by employers named “UAX Skill School.” This model has been created by using Coursera platform and selecting online massive open courses (MOOCS) for being adapted to different subjects to ensure the students develop the most useful soft skills. This research summarizes the process and results of the adaptation and implementation of the MOOC “Learning How to Learn” from the Coursera platform in Parasitic Diseases subject which complements and enhances the learning process of Veterinary students. Methods During 2022–2023, 2023–2024, and 2024–2025 academic years, the MOOC “Learning How to Learn” has been integrated and adapted in the subject of Parasitic Diseases in 7 practical sessions before students performed the MOOC and achieved certification. Medallia survey data has been conducted to review student insights. Results Student participation has been over 90%, the dropout rate is less than 2.8% and the performance and learning experience per academic year have reached. Likewise, the invested time (6.41, 5.09, and 4.19 h respectively) and the global participation 94.16%, as well as the conformity of the students with respect to the learning process with grades from 7.85 before the introduction of the pedagogical model to 8.59 after it. Discussion Innovative methodologies centered on technology are valuable for developing soft skills as evidenced by the implementation of the MOOC “Learning How to Learn” within the context of Parasitic Diseases. Key success factors include the adaptation and integration of these resources into the curriculum. Further research is required to attain a comprehensive understanding of the potential impact of this MOOC on the learning outcomes and soft skills development of Veterinary students.

The characterization of sperm and seminal plasma proteins is essential for understanding bull fertility and optimizing reproductive success in buffalo bulls. Despite its importance, the reproductive proteomic of Toraya buffalo, an indigenous breed in Indonesia, remains largely unexplored. This study aimed to examine the seminal plasma and sperm proteins of Toraya buffalo to uncover those critical for reproductive functions. Semen samples were collected from eight Toraya buffalo bulls aged 4 to 10 years. Protein profiling was performed using one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D-SDS-PAGE), followed by in-gel digestion and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Bioinformatics tools, including UniProt, PANTHER, DAVID, and STRING, were utilized to identify and annotate the detected proteins. This study successfully identified four key reproductive proteins: ADAM32 in seminal plasma and ZPBP, SPACA3, and CCDC136 in sperm. These proteins are essential for sperm motility, energy production, and acrosome formation, which are critical processes for fertilization. Additionally, many identified proteins were associated with metabolic pathways, particularly the tricarboxylic acid (TCA) cycle, which plays a fundamental role in energy supply for sperm function. In conclusion, this study offers the first comprehensive proteomic identification of seminal plasma and sperm proteins associated with reproductive functions in the Toraya buffalo. The findings highlight the presence of key proteins in sperm, including ZPBP, SPACA3, and CCDC136, as well as the identification of ADAM32 in seminal plasma, contributing to a deeper understanding of buffalo reproductive biology.

Zearalenone (ZEA), a prevalent mycotoxin in animal feeds, is known to disrupt normal ovarian development and function due to its estrogenic activity. This study investigates the toxic effects of ZEA on the ovaries of meat rabbits and explores the underlying mechanisms. Ninety healthy 41-day-old Hyla male rabbits were randomly assigned into three groups. The control group received a basal diet, while the experimental groups were fed basal diets supplemented with 300 and 600 μg/kg ZEA, respectively. Each group consisted of 30 replicates, with one rabbit per replicate, and the experimental period lasted 42 days. The results showed that, compared to the control group, the ovarian index was significantly increased in the 600 μg/kg ZEA supplementation group (p < 0.05). In addition, ovarian tissue exhibited pathological changes, including follicular dilatation, thinning of the follicular granulosa, punctate necrosis of granulosa cells, deep stained cytosolic nuclei, and nuclear fragmentation. Compared to the control group, the 600 μg/kg ZEA supplementation group exhibited significantly elevated blood levels of gonadotropin-releasing hormone, luteinizing hormone, estradiol, malondialdehyde (MDA), and interleukin 1β (IL-1β) (p < 0.05). Conversely, total antioxidant power (TAOC) and glutathione peroxidase (GSH-Px) activities were significantly reduced in this group (p < 0.05). The level of MDA in the ovarian tissue of rabbits in the 600 μg/kg ZEA supplementation group was significantly elevated compared to the control group, while the activities of GSH-Px and TAOC were significantly reduced (p < 0.05). Moreover, the expression levels of luteinizing hormone receptor mRNA, heat shock protein 70 mRNA, tumor necrosis factor-α mRNA, and IL-1β mRNA in the ovarian tissue significantly increased, whereas the expression of copper and zinc superoxide dismutase mRNA was significantly decreased compared to the control group (p < 0.05). In conclusion, supplementation with 600 μg/kg ZEA induces oxidative stress and inflammatory responses in the ovaries of meat rabbits by modulating the expression of related genes. These effects disrupt ovarian development, cause pathological changes, and impair the secretion of reproductive hormones. This study is the first to report the toxic effects of ZEA on the ovaries of Hyla rabbits and provides preliminary insights into its underlying mechanisms.

This study investigated the effect of different ratios of quinoa-to-alfalfa silage on the fermentation parameters, methane production, and rumen microbial community composition during in vitro fermentation trials. The objective was to evaluate the potential of quinoa as a viable silage material. Five treatment groups were set up with varying quinoa proportions of 0, 30, 50, 70, and 100%, and stored 60 days. The results showed that increasing the quinoa proportion in the alfalfa-quinoa mixed silage resulted in a decrease in concentrations of propionate, isobutyrate, isovalerate, and the methane (CH4) fraction of total gas emissions (p < 0.05). Conversely, dry matter digestibility, total volatile fatty acid (TVFA) concentration, acetate concentration, acetate to propionate ratio, butyrate concentration, cumulative CH4 emissions, and total gas production increased (p < 0.05). At the phylum level, the relative abundance of Spirochaetota decreased linearly (p < 0.05), while Verrucomicrobiota increased (p < 0.05). At the genus level, the relative abundance of CAG 873, Prevotella, Acinetobacter, Treponema D, RUG11690, and Ruminococcus E decreased linearly (p < 0.05), whereas the relative abundance of Bact 11, Limimorpha, F23 D06, Advenella, and unclassified bacteria increased linearly (p < 0.05). In summary, the inclusion of quinoa in alfalfa silage alters the fiber structure of the feed and significantly affects its nutritional composition, in vitro fermentation parameters, methane production, and microbial community composition. These findings offer valuable insights for optimizing ruminant feed.

The aim of this study was to evaluate the effects of acupuncture points VG26 and K1 on the reversal of apnea and the elevation of heart and respiratory rates in neonatal dogs after birth. Sixty neonates who were apneic, bradycardic, bradypneic or dyspneic were divided into three groups: VG26 acupuncture point (GVG26), K1 acupuncture point (GK1), and control (CG). Data on heart rate (HR), respiratory rate (RR) and Apgar score were collected. The moments of analysis were after 1 min of tactile respiratory stimulus (M1); after stimulation with the acupuncture points (M2); and 10 min after the last assessment (M3). After the VG26 and K1 were used, a significant increase in HR was observed between M1 and M2, medians (25% quartile–75% quartile), respectively: GVG26 136 and 202 bpm; GK1 147 and 195 bpm; GC 168 and 187 bpm. Significant clinical improvement was also observed in RR, with a median at M1 and M2, respectively: GVG26 16 and 28 mpm; GK1 18 and 32 mpm; GC 32 and 40 mpm. For the Apgar score, significant differences were observed in the mean scores between M1 and M3 in the GVG26, GK1 and CG groups. The VG26 and K1 acupuncture points are effective in neonatal puppies, leading to improvements in the respiratory pattern and increases in the HR, RR and Apgar score, can be used in neonatal resuscitation at birth.

Introduction The use of transvaginal ultrasound-guided follicle aspiration (TVA) for oocyte collection has become a widely used procedure in horses for in vitro embryo production (IVEP). The TVA procedure is characterized by various manipulations, which are physical restraint, perineal preparation, transrectal palpation, insertion of an intravaginal device, and needle punctures. These repeated transvaginal and transovarian punctures have raised concerns about their potential effects on mare welfare. Our study aimed to investigate the effects of TVA manipulations, and especially puncturing, on pain and stress in mares in a commercial set-up. Methods Therefore, eight mares were subjected to three TVA procedures: two with puncturing (P⁺) and one without (P⁻, control). Before, during and after all TVA procedures, blood was collected for serum glucose, lactate and cortisol levels, and facial pain scores were assessed. An electrocardiogram (ECG) was recorded for each mare before and during the procedure to measure heart rate (HR) and heart rate variability (HRV). Parameters in response to manipulations and puncturing were analyzed using linear mixed effect models for each outcome variable at different timepoints during the procedure. Results Results revealed that puncturing during TVA did not significantly influence serum metabolite levels, facial pain scores, HR, or HRV over the complete procedure (p > 0.05). Notably, HR did not increase at the moment of puncturing (p > 0.05), and no significant changes in HRV parameters between P⁺ and P⁻ were detected (p > 0.05). Both P⁺ and P⁻ procedures triggered significant increases in cortisol and lactate levels, facial pain scores, and HR during restraint in stocks and perineal preparation compared to the day before and the day after TVA (p < 0.05). Interestingly, even without puncturing, manipulations in the P⁻ procedure were sufficient to induce significant elevations in metabolite levels and facial pain scores compared to the day before and after (p < 0.05). Discussion These results indicate that the TVA procedure induces stress responses in mares, predominantly associated with all manipulations specific to TVA, while the effect of puncturing itself was minimal. All effects were acute, with parameters returning to baseline when measured 24 h later.