Journal of Chromatography A (J Chrom)

Publisher: Elsevier

Journal description

The Journal of Chromatography A publishes papers on all aspects of separation science including chromatography, electrochromatography, electrophoresis, hyphenated and other multi-dimensional techniques, sample preparation as well as detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with chromatographic and electrophoretic theory, instrumental developments and their analytical and preparative applications. Section A covers all areas except biomedical sciences and biomedical applications of separation science, which are published in section B: Biomedical Sciences and Applications. Electronic version.

Current impact factor: 4.17

Impact Factor Rankings

2016 Impact Factor Available summer 2017
2014 / 2015 Impact Factor 4.169
2013 Impact Factor 4.258
2012 Impact Factor 4.612
2011 Impact Factor 4.531
2010 Impact Factor 4.194
2009 Impact Factor 4.101
2008 Impact Factor 3.756
2007 Impact Factor 3.641
2006 Impact Factor 3.554
2005 Impact Factor 3.096
2004 Impact Factor 3.359
2003 Impact Factor 2.922
2002 Impact Factor 3.098
2001 Impact Factor 2.793
2000 Impact Factor 2.551
1999 Impact Factor 2.52
1998 Impact Factor 2.321
1997 Impact Factor 2.697
1996 Impact Factor 2.457
1995 Impact Factor 2.296
1994 Impact Factor 2.523

Impact factor over time

Impact factor

Additional details

5-year impact 4.30
Cited half-life 7.90
Immediacy index 0.62
Eigenfactor 0.07
Article influence 0.85
Website Journal of Chromatography A website
ISSN 1873-3778

Publisher details


  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • Authors pre-print on any website, including arXiv and RePEC
    • Author's post-print on author's personal website immediately
    • Author's post-print on open access repository after an embargo period of between 12 months and 48 months
    • Permitted deposit due to Funding Body, Institutional and Governmental policy or mandate, may be required to comply with embargo periods of 12 months to 48 months
    • Author's post-print may be used to update arXiv and RepEC
    • Publisher's version/PDF cannot be used
    • Must link to publisher version with DOI
    • Author's post-print must be released with a Creative Commons Attribution Non-Commercial No Derivatives License
    • Publisher last reviewed on 03/06/2015
  • Classification

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: A theory of chromatography is developed for partially cyclic polymers of tadpole- and manacle-shaped topological structures. We present exact equations for the distribution coefficient K at different adsorption interactions; simpler approximate formulae are also derived, relevant to the conditions of size-exclusion, adsorption, and critical chromatography. Theoretical chromatograms of heterogeneous partially cyclic polymers are simulated, and conditions for good separation by topology are predicted. According to the theory, an effective SEC-radius of tadpoles and manacles is mostly determined by the molar mass M, and by the linear-cyclic composition. In the interactive chromatography, the effect of molecular topology on the retention becomes significant. At the critical interaction point, partial dependences K(M lin) and K(M ring) are qualitatively different: while being almost independent of M lin, K increases with M ring. This behavior could be realized in critical chromatography - for separation of partially cyclic polymers by the number and molar mass of cyclic elements.
    No preview · Article · Feb 2016 · Journal of Chromatography A
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    ABSTRACT: The ionic strength is one of the key parameters for optimizing CE separations. However, only a few data are available in the literature about the ionic strength effect on the separation of proteins. The effect of ionic strength on separation performances is rather complex since many different parameters are involved: such as the protein effective mobility, the electroosmotic mobility, the separation efficiency via the electromigration dispersion, as well as the viscosity and temperature of the background electrolyte. In the present work, the influence of ionic strength on the electrophoretic separation of five model proteins has been investigated in acidic conditions, on successive multi-ionic layers coated capillary, in counter-electroosmotic mode with anodic electroosmotic flow. The decrease in effective and electroosmotic mobilities with increasing ionic strength were compared using the slope-plot approach, which is very helpful for understanding the observed changes in apparent selectivity and resolution. The relative decrease of the protein effective mobility was about 30%-40% of the mobility determined at 5 mM ionic strength per ionic strength decade. It was found that relatively low ionic strength (∼5–10 mM) was preferable to optimize the overall separation of the five model proteins.
    No preview · Article · Feb 2016 · Journal of Chromatography A
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    ABSTRACT: A simple approach was developed for rapid preparation of polymeric monolithic columns in UV-transparent fused-silica capillaries via photoinitiated thiol-acrylate polymerization of pentaerythritol diacrylate monostearate (PEDAS) and trimethylolpropane tris(3-mercaptopropionate) (TPTM) within 10 min, in which the acrylate homopolymerized and copolymerized with the thiol simultaneously. The morphology, permeability and chromatographic performance of the resulting poly(PEDAS-co-TPTM) monoliths were studied. It could be observed from SEM that the morphology of poly(PEDAS-co-TPTM) monolith was rather different from that of poly(PEDAS) monolith, which was fabricated via photo-induced free radical polymerization using PEDAS as the sole monomer. Compared with poly(PEDAS) monolith, poly(PEDAS-co-TPTM) monolith possessed better permeability when they were fabricated under the same preparation conditions. By adjusting the composition of porogenic solvents, poly(PEDAS-co-TPTM) monolith exhibited lower plate heights (15.7–17.7 μm) than poly(PEDAS) monolith (19.1–37.9 μm) in μLC. In addition, 66 unique peptides were positively identified on poly(PEDAS-co-TPTM) monolith when tryptic digest of four proteins was separated by μLC-MS/MS, demonstrating its potential in proteome analysis.
    No preview · Article · Jan 2016 · Journal of Chromatography A
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    ABSTRACT: A very convenient, sensitive and precise solid phase extraction (SPE) system was developed for enrichment and determination of ultra-trace of cadmium ion in water and plant samples. This method was based on the retention of cadmium(II) ions by L-cystine adsorbed in Y-zeolite and carry out in a packed mini-column. The retained cadmium ions then were eluted and determined by flame atomic absorption spectrometry. The scanning electron microscopy (SEM), powder X-ray diffraction (XRD) and Fourier Transform Infrared (FT-IR) spectroscopy techniques were applied for the characterization of cystine modified zeolite (CMZ). Some experimental conditions affecting the analytical performance such as pH, eluent type, concentration of sample, eluent flow rate and also the presence of interfering ions were investigated. The calibration graph was linear within the range of 0.1–7.5 ng mL−1 and limit of detection was obtained 0.04 ng mL−1 with the preconcentration factor of 400. The relative standard deviation (RSD) was obtained 1.4%, indicating the excellent reproducibility of this method. The proposed method was successfully applied for the extraction and determination of cadmium(II) ion in black tea, cigarette’s tobacco and also various water samples.
    No preview · Article · Jan 2016 · Journal of Chromatography A
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    ABSTRACT: A new concept of using thin-layer chromatography to sample preparation for the quantitative determination of solute/s followed by instrumental techniques is presentedThin-layer chromatography (TLC) is used to completely separate acetaminophen and its internal standard from other components (matrix) and to form a single spot/zone containing them at the solvent front position (after the final stage of the thin-layer chromatogram development). The location of the analytes and internal standard in the solvent front zone allows their easy extraction followed by quantitation by HPLC The exctraction procedure of the solute/s and internal standard can proceed from whole solute frontal zone or its part without lowering in accuracy of quantitative analysis.
    No preview · Article · Jan 2016 · Journal of Chromatography A
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    ABSTRACT: Here, a novel β-cyclodextrin (β-CD) based trace matrix solid-phase dispersion (MSPD) method was developed to extract isomers from different honeysuckle samples using ultrahigh performance liquid chromatography. The structures of the analytes were characterized and determined via quadrupole time-of-flight tandem mass spectrometry and nuclear magnetic resonance. Under optimum conditions, 25 mg of sample was dispersed with 75 mg of β-CD, and 0.5 mL of methanol was used as the elution solvent. The proposed method produced a good linearity (r2>0.99) for the isomers, with limits of detection ranging from 1.62 to 3.33 ng/mL. The average recovery values that were obtained from analyzing the spiked samples were calculated to be in the range of 87.04%-105.20%. Moreover, the trace MSPD method required less reagents and a shorter extraction time in comparison with the traditional pharmacopoeia method and other reported approaches. The developed method was successfully applied for the determination of three real honeysuckle samples that were collected from different cultivated areas.
    No preview · Article · Jan 2016 · Journal of Chromatography A
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    ABSTRACT: Competitive adsorption isotherm of ibuprofen enantiomers on Chiralcel OD stationary phase at 298 K was determined by the application of inverse method. Transport dispersive (TD) chromatography model was used to describe mass balances of the enatiomers. Axial dispersion and mass transfer coefficients were estimated from a series of linear pulse experiments. It was found that the overloaded elution profile of total concentration of racemic ibuprofen cannot be satisfactorily fitted by substituting bi-Langmuir model, the most widely used isotherm model for enantiomers, into TD model and tuning the isotherm parameters. UV-CD dual detector setup was then applied to obtain the individual overloaded elution profiles of both enantiomers. The more informative experimental data revealed non-Langmuirian adsorption behaviour of ibuprofen enantiomers on chiralcel OD stationary phase. Two analytical binary isotherm models, both accounting for adsorbate-adsorbate interactions and having the feature of inflection points, were then evaluated. A comparison between quadratic model and Moreau model showed that the former gives better fitting results. The six parameters involved in quadratic model were determined stepwisely. Three of them were first obtained by fitting overloaded elution profiles of S-ibuprofen. The other three were then acquired by fitting overloaded elution profiles of both enantiomers recorded by UV-CD dual detector for racemic ibuprofen. A further attempt was also made at reducing the number of quadratic model parameters.
    No preview · Article · Jan 2016 · Journal of Chromatography A
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    ABSTRACT: This work reports a chemometrics-assisted high performance liquid chromatography-diode array detection (HPLC-DAD) strategy to solve varying interfering patterns from different chromatographic columns and sample matrices for the rapid simultaneous determination of six synthetic colorants in five kinds of beverages with little sample pretreatment. The investigation was performed using two types of LC columns under the same elution conditions. Although analytes using different columns have different co-elution patterns that appear more seriously in complex backgrounds, all colorants were properly resolved by alternating trilinear decomposition (ATLD) method and accurate chromatographic elution profiles, spectral profiles as well as relative concentrations were obtained. The results were confirmed by those obtained from traditional HPLC-UV method at a particular wavelength and the results of both methods were consistent with each other. All results demonstrated that the proposed chemometrics-assisted HPLC-DAD method is accurate, economical and universal, and can be promisingly applied to solve varying interfering patterns from different chromatographic columns and sample matrices for the analysis of complex food samples.
    No preview · Article · Jan 2016 · Journal of Chromatography A
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    ABSTRACT: This study reports a new headspace gas chromatographic method (HS-GC) for the determination of water solubility of low volatility liquid organic compounds (LVLOs). The HS-GC analysis was performed on a set of aqueous solutions containing a range of concentrations of toluene-spiked (as a tracer) LVLOs, from under-saturation to over-saturation. A plot of the toluene tracer GC signal vs. the concentration of the LVLO results in two lines of different slopes that intersect at the concentration corresponding to the compound's solubility in water. The results showed that the HS-GC method has good precision (RSD < 6.3%) and good accuracy, in which the relative deference between the data measured by the HS-GC method and the reference method were within 6.0%. The HS-GC method is simple and particularly suitable for measuring the solubility of LVLOs at elevated temperatures. This approach should be of special interest to those concerned about the impact of the presence of low-volatility organic liquids in waters of environmental and biological systems.
    No preview · Article · Jan 2016 · Journal of Chromatography A
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    ABSTRACT: In this study, a robust method for quick screening, confirmation and quantification analysis of eight fluoroalkyl sulfonates in surface riverine samples was developed using ultra-high performance liquid chromatography-high resolution mass spectrometer (LC-Orbitrap Tribrid HRMS). Weak anion exchange solid phase extraction was optimized to maximum recover perfluoroalkyl sulfonates (PFSAs), fluorotelomer sulfonates and the emerging 6:2 chlorinated polyfluoroalkyl ether sulfonate at the same time. Both qualitative and quantitative purposes could be achieved by simultaneous acquiring full-scan mass spectrum (MS1) and data-dependent MS2 data. The LC-Orbitrap Tribrid HRMS method showed competent method detection limits for all analytes (7.1–62 pg/L) compared with the triple quadrupole mass spectrometry (LC–MS/MS) quantification method (12–54 pg/L), and satisfactory method validation results were also obtained in linearity (R2 > 0.999), trueness (88–118%), precision (2–17%) and recovery (62–103%). A good correlation (R > 0.999) was found between the sets of quantified PFSA residue concentrations in thirteen estuary river samples by both the LC-Orbitrap Tribrid HRMS (0.19–440 ng/L) and LC–MS/MS (0.11–424 ng/L) methods, indicating that Orbitrap Tribrid HRMS could be used for reliable quantitative analysis purpose. Moreover, the LC-Orbitrap Tribrid HRMS method could also benefit from its high mass resolution characteristic to eliminate potential environment interferents (e.g. taurodeoxycholate) and to quantify all PFSA isomers based on full-scan MS1 chromatogram at a narrow MS window (5 part per million).
    No preview · Article · Jan 2016 · Journal of Chromatography A