Reproductive biology (REPROD BIOL)

Publisher: Towarzystwo Biologii Rozrodu

Current impact factor: 1.52

Impact Factor Rankings

2016 Impact Factor Available summer 2017
2014 / 2015 Impact Factor 1.524
2013 Impact Factor 1.048
2012 Impact Factor 1.222
2011 Impact Factor 1.921
2010 Impact Factor 1.5
2009 Impact Factor 0.882

Impact factor over time

Impact factor

Additional details

5-year impact 1.49
Cited half-life 6.30
Immediacy index 0.29
Eigenfactor 0.00
Article influence 0.39
Other titles Reproductive Biology
ISSN 1642-431X
OCLC 168875590
Material type Periodical
Document type Journal / Magazine / Newspaper

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Exposure to di-(2-ethylhexyl) phthalate (DEHP) induces spermatogenic disturbance (SD) through oxidative stress, and affects the immune system by acting as an adjuvant. Recently, we reported that in mice, a low dose of DEHP, which did not affect spermatogenesis, was able to alter the testicular immune microenvironment. Experimental autoimmune orchitis (EAO) can be induced by repeated immunization with testicular antigens, and its pathology is characterized by production of autoantibodies and SD. In the present study, we investigated the effect of a low-dose DEHP on the susceptibility of mice to EAO. The exposure to DEHP-containing feed (0.01%) caused a modest functional damage to the blood-testis barrier (BTB) with an increase in testicular number of interferon gamma (IFN-γ)-positive cells and resulted in the production of autoantibodies targeting haploid cells, but did not affect spermatogenesis. While only single immunization with testicular antigens caused very mild EAO, the concurrent DEHP exposure induced severe EAO with significant increases in number of interferon gamma-positive cells and macrophages, as well as lymphocytic infiltration and serum autoantibody titer accompanied by severe SD. To summarize, the exposure of mice to the low-dose DEHP does not induce significant SD, but it may cause an increase in IFN-γ positive cells and modest functional damage to the BTB in the testis. These changes lead to an autoimmune response against haploid cell autoantigens, resulting in increased susceptibility to EAO.
    No preview · Article · Sep 2015 · Reproductive biology
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    ABSTRACT: The pro-inflammatory T helper (Th)-1 cytokines, tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), are immunological factors relevant at the feto-maternal interface and involved in the pathophysiology of implantation disorders. The synergistic action of the two cytokines has been described with regard to apoptotic cell death and inflammatory responses in different cell types, but little is known regarding the human endometrium. Therefore, we examined the interaction of TNF-α and IFN-γ in human endometrial stromal cells (ESCs). ESCs were isolated from specimens obtained during hysterectomy and decidualized in vitro. Cells were incubated with TNF-α, IFN-γ or signaling-inhibitor. Insulin-like growth factor binding protein (IGFBP)-1, prolactin (PRL), leukemia inhibitory factor (LIF), interleukin (IL)-6, IL-8, regulated on activation normal T-cell expressed and secreted protein (RANTES) and monocyte chemotactic protein (MCP)-1 were measured using ELISA and real-time RT-PCR. Nuclear factor of transcription (NF)-κB and its inhibitor (IκBα) were analyzed by in-cell western assay and transcription factor assay. TNF-α inhibited and IFN-γ did not affect the decidualization of ESCs. In contrast, IFN-gamma differentially modulated the stimulating effect of TNF-alpha on cytokines by enhancing IL-6, RANTES and MCP-1 and attenuating LIF mRNA expression. These effects were time- and dose-dependent. IFN-γ had no impact on the initial activation of NF-κB signaling. Histone-deacetylase activity was involved in the modulating effect of IFN-γ on RANTES secretion. These observations showed a distinct pattern of interaction of the Th-1 cytokines, TNF-α and IFN-γ in the human endometrium, which could play an important role in the pathophysiology of implantation disorders.
    No preview · Article · Sep 2015 · Reproductive biology
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    ABSTRACT: Glucose is suggested to play a key role in motility hyperactivation of mammalian spermatozoa. The current study aimed to investigate the modulatory effects of silymarin and/or a protein kinase A (PKA) inhibitor (H-89) on glucose-mediated motility parameters of mouse spermatozoa. Spermatozoa were incubated in HEPES medium containing normal (NG; 5.5mM) or high (HG; 25mM) glucose concentration. The results of computer-assisted analysis showed that samples incubated in HG resulted in a larger (p<0.05) percentage of motile spermatozoa at 120min (59.5±14.8% vs. 34.0±4.4%) compared to those incubated in NG. The average pathway velocity (VAP), curvilinear velocity (VCL), and straight-line velocity (VSL) exhibited similar patterns at 60 and 120min when incubated in HG (p<0.05). Treatments with silymarin (5, 10, 20μg/mL) did not significantly affect sperm motility under NG conditions, but decreased the HG-enhanced motility, VAP, and VCL at 120min (p<0.05). H-89 (30μM) reduced (p<0.05) motility at 30min examined in the NG or HG medium. At 90min, H-89 also reduced (p<0.05) the HG-enhanced motility of spermatozoa incubated with or without 20μg/mL silymarin by 49% or 32%, respectively. In conclusion, the H-89-inhibition of glucose-mediated mouse sperm motility and certain types of velocity suggests that the glycolysis-PKA pathway is involved in the regulation of sperm motility. Silymarin may maintain sperm motility under NG conditions, but it inhibits glucose-activated sperm motility.
    No preview · Article · Sep 2015 · Reproductive biology
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    ABSTRACT: In the present study, the effects of tacrolimus on the activity and expression of tissue factor (TF) were investigated in the ovarian ischemia–reperfusion induced injury in rats. Twenty-eight female rats (8–12 weeks, 300–350 g) were divided into four groups: control, ischemia–reperfusion (IR), tacrolimus treated before ischemia (TBI), and tacrolimus treated before reperfusion (TBR) groups (n = 7/per group). TF activity was measured using Quick's method, whereas TF expression was examined immunohistochemically. TF activity was significantly higher in all treated groups compared with the control group. Strong ovarian TF expression was demonstrated in the IR and TBR groups. Moreover, tacrolimus decreased TF activity in the TBI group compared with the IR group. The decreased activity of TF in the ovarian IR model may prevent IR-related inflammation during transplant procedure
    No preview · Article · Jul 2015 · Reproductive biology
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    ABSTRACT: Carnitine is an important factor in the initiation of progressive sperm motility and end stage of sperm maturation. The compound is transported by an organic cation/carnitine transporter (OCTN2), which is expressed in the male reproductive system. OCTN2 is encoded by SLC22A5 gene with proven −207C>G functional polymorphism. The aim of the case–control study was to investigate a potential association between the −207C>G SLC22A5 polymorphism and male infertility. The −207C>G SLC22A5 polymorphism was determined by means of TaqMan assay in 206 infertile Caucasian males and 256 ethnically matched controls. Besides genotyping study, sperm mitochondrial function was assessed using NADH-dependent NBT assay. The distribution of SLC22A5 genotypes in infertile men was as follows: CC – 29.6%, CG – 53.9%, GG – 16.5% and in fertile men: CC – 32.0%, CG – 50.0%, GG – 18.0%, and was comparable in both evaluated groups. Likewise, the studied polymorphism did not affect sperm mitochondrial function. The results of the current study demonstrated that −207C>G polymorphism of the SLC22A5 gene is not associated with male infertility.
    No preview · Article · Jun 2015 · Reproductive biology
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    ABSTRACT: The acquisition of ovulatory capacity in the growing dominant follicle (DF) of dromedary camels was examined in the current study. Ovulation occurred in response to hCG (1500 IU) in 27.3%, 58.3% or 100% of camels with follicles of 9, 10 or 11 mm diameter, respectively. A high dose of hCG (4500 IU) resulted in ovulation of 77.8% and 100% of camels with follicles of 9 and 10 mm, respectively. In naturally mated animals, ovulation occurred in 36.4% and 92.8% of camels with 10 and 11 mm follicles, respectively.
    No preview · Article · Jun 2015 · Reproductive biology
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    ABSTRACT: Autophagy is activated by environment unfavorable for survival and requires Atg9a protein. Mice heterozygous for p57Kip2, devoid of the imprinted paternal allele (p57Kip2+/−), are known to develop hypertension during pregnancy. To determine whether fetal Atg9a is involved in the intrauterine survival and growth of fetal mice, this study was performed on Atg9a heterozygous (Atg9a+/−) pregnant mice with and without p57Kip2+/−. The pregnant mice heterozygous for both knockout alleles of Atg9a and p57Kip2 (Atg9a+/−/p57Kip2+/−), but not those heterozygous for Atg9a alone, developed hypertension during pregnancy. Placental expression of Atg9a mRNA was significantly decreased in the Atg9a−/− mice compared to Atg9a+/− or Atg9a+/+ mice. The Atg9a−/− fetal mice exhibited significantly retarded growth and were more likely to die in utero compared to Atg9a+/+ and Atg9a+/− fetal mice. Growth retardation was observed in the presence of maternal hypertension in Atg9a−/− fetal mice. These results suggest that Atg9a−/− fetal mice from pregnant dams heterozygous for both knockout alleles of Atg9a and p57Kip2 are more susceptible to hypertensive stress than fetuses with intact autophagic machinery.
    No preview · Article · Jun 2015 · Reproductive biology
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    ABSTRACT: In order to simplify management of early pregnancy loss, our goal was to elucidate predictors of successful medical management of miscarriage with a single dose of misoprostol. In this secondary analysis of data from a multicenter randomized controlled trial, candidate biomarkers were compared between 49 women with missed abortion who succeeded in passing their pregnancy with a single dose of misoprostol and 46 women who did not pass their pregnancy with a misoprostol single dose. We computed the precision of trophoblastic protein and hormone concentrations to discriminate between women who succeed or fail single dose misoprostol management. We also included demographic factors in our analyses. We found overlap in the concentrations of the individual markers between women who succeeded and failed single-dose misoprostol. However, hCG levels ≥4000mIU/mL and ADAM-12 levels ≥2500pg/mL were independently associated with complete uterine expulsion after one dose of misoprostol in our population. A multivariable logistic model for success included non-Hispanic ethnicity and parity <2 in addition to hCG ≥4000mIU/mL and ADAM-12 ≥2500pg/mL and had an area under the receiver operating characteristic (ROC) of 0.81 (95% confidence interval: 72-90%). Categorizing women with a predicted probability of ≥0.65 resulted in a sensitivity of 75.0%, specificity 77.1% and positive predictive value of 81.8%. While preliminary, our data suggest that serum biomarkers, especially when combined with demographic characteristics, may be helpful in guiding patient decision-making regarding the management of early pregnancy failure (EPF). Further study is warranted. Copyright © 2015 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.
    No preview · Article · Feb 2015 · Reproductive biology