Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Publisher: Elsevier

Current impact factor: 1.97

Impact Factor Rankings

2016 Impact Factor Available summer 2017
2014 / 2015 Impact Factor 1.966
2013 Impact Factor 2.371
2012 Impact Factor 2.167
2011 Impact Factor 2.235
2010 Impact Factor 2.134
2009 Impact Factor 2.196
2008 Impact Factor 1.709
2007 Impact Factor 1.863
2006 Impact Factor 1.553
2005 Impact Factor 1.351
2004 Impact Factor 1.635
2003 Impact Factor 1.556
2002 Impact Factor 1.274
2001 Impact Factor 1.026
2000 Impact Factor 0.883
1999 Impact Factor 0.916
1998 Impact Factor 0.645
1997 Impact Factor 0.748
1996 Impact Factor 0.618
1995 Impact Factor 0.531
1994 Impact Factor 0.64
1993 Impact Factor 0.619
1992 Impact Factor 0.623

Impact factor over time

Impact factor

Additional details

5-year impact 2.26
Cited half-life >10.0
Immediacy index 0.51
Eigenfactor 0.01
Article influence 0.66
Other titles Comparative biochemistry and physiology., Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Comparative biochemistry and physiology., Molecular and integrative physiology, Molecular & integrative physiology, Comp. biochem. physiol., CBP., Comparative biochemistry and physiology
ISSN 1531-4332
OCLC 41929819
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details


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    • Publisher last reviewed on 03/06/2015
  • Classification

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Using fish scales in which osteoclasts and osteoblasts coexist on the calcified bone matrix, we examined the effects of low-intensity pulsed ultrasound (LIPUS) on both osteoclasts and osteoblasts. At 3 h of incubation after LIPUS treatment, osteoclastic markers such as tartrate-resistant acid phosphatase (TRAP) and cathepsin K mRNA expressions decreased significantly while mRNA expressions of osteoblastic markers, osteocalcin, distal-less homeobox 5, runt-related transcription factor 2a, and runt-related transcription factor 2b, increased significantly. At 6 and 18 h of incubation, however, both osteoclastic and osteoblastic marker mRNA expression did not change at least present conditions. Using GeneChip analysis of zebrafish scales treated with LIPUS, we found that cell death-related genes were upregulated with LIPUS treatment. Real-time PCR analysis indicated that the expression of apoptosis-related genes also increased significantly. To confirm the involvement of apoptosis in osteoclasts with LIPUS, osteoclasts were induced by autotransplanting scales in goldfish. Thereafter, the DNA fragmentation associated with apoptosis was detected in osteoclasts using the TUNEL (TdT-mediated dUTP nick-end labeling) method. The multi-nuclei of TRAP-stained osteoclasts in the scales were labeled with TUNEL. TUNEL staining showed that the number of apoptotic osteoclasts in goldfish scales was significantly elevated by treatment with LIPUS at 3 h of incubation. Thus, we are the first to demonstrate that LIPUS directly functions to osteoclasts and to conclude that LIPUS directly causes apoptosis in osteoclasts shortly after exposure.
    No preview · Article · Feb 2016 · Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
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    ABSTRACT: It is well known that many anoxia/hypoxia tolerant species when exposed to anoxia/hypoxia respond by increasing the activity/expression of antioxidant enzymes and/or glutathione levels – a phenomenon called “preparation for oxidative stress” (POS). This phenomenon was also observed during freezing exposure, severe dehydration, aerial exposure of water-breathing animals and estivation. However, as far as we know, there is no analysis available of the prevalence of POS among animal species. A major problem is the very definition of POS, since many animal species show both increases and decreases of antioxidants during low oxygen stress and estivation. Therefore, we established three different criteria; from inclusive to restrictive and analyzed how widespread the POS phenomenon is in the animal kingdom. We analyzed all available papers in several databases about the modulation of antioxidant defenses during oxygen deprivation or estivation. Based on the magnitude of change (as % change) during the specific low oxygen stresses or estivation, we classified each species as POS-positive, POS-negative or POS-neutral, considering the three different criteria for each tissue. The prevalence of POS-positive animals (102 species from 8 phyla) was stress-dependent: in estivation and dehydration it was 91–100%, while in hypoxia it was 37.5–53%, depending on the criteria. In the case of air exposure, anoxia and freezing the proportions of POS-positive species were 54–77%, 64–77% and 75–86%, respectively. Overall, the prevalence of POS was 58 to 68% when all stresses and all species were analyzed together. The results indicate the key importance of POS as a survival strategy of animals exposed to freezing, dehydration and estivation, and, to a lesser extent, to oxygen deprivation itself (i.e. hypoxia and anoxia).
    No preview · Article · Feb 2016 · Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
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    ABSTRACT: The macronutrient composition of diets is a very important factor in the regulation of body weight and metabolism. Several lines of research in mammals have shown that macronutrients differentially regulate metabolic hormones, including ghrelin and nesfatin-1 that has opposing effects on energy balance. This study aimed to determine whether macronutrients modulate the expression of ghrelin and the nucleobindin-2 (NUCB2) encoded nesfatin-1 in goldfish (Carassius auratus). Fish were fed once daily on control, high-carbohydrate, high-protein, high-fat and very high-fat diets for 7 (short-term) or 28 (long-term) days. The expression of preproghrelin, ghrelin O-acyl transferase (goat), growth hormone secretagogue receptor 1 (ghs-r1) and nucb2/nesfatin-1 mRNAs was quantified in the hypothalamus, pituitary, gut and liver. Short-term feeding with fat-enriched diets significantly increased nucb2 mRNA levels in hypothalamus and liver, preproghrelin, goat and ghs-r1 expression in pituitary, and ghs-r1 expression in gut. Fish fed on a high-protein diet exhibited a significant reduction in preproghrelin and ghs-r1 mRNAs in the liver. After long-term feeding, fish fed on high-carbohydrate and very high-fat diets had significantly increased preproghrelin, goat and ghs-r1 expression in pituitary. Feeding on a high-carbohydrate diet also upregulated goat and ghs-r1 transcripts in gut, while feeding on a high-fat diet elicited the same effect only for ghs-r1 in liver. Nucb2 expression increased in pituitary, while decreased in gut after long-term feeding of a high-protein diet. Collectively, these results show for the first time in fish that macronutrients differentially regulate the expression of the ghrelinergic and NUCB2/nesfatin1 systems in central and peripheral tissues of goldfish.
    No preview · Article · Jan 2016 · Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
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    ABSTRACT: Most fish species possess duplicate leptin genes (LEP). Mandarin fish (Siniperca chuatsi) leptin A gene (sLEP-A) have been cloned in the previous study. In the present study, we cloned and characterized leptin B gene (sLEP-B) in mandarin fish, including a 471 bp open reading frame (ORF) encoding a 158-amino acid protein. The three-dimensional (3D) structural model of sLEP-B protein showed a highly conserved of tertiary structure similar to that of other vertebrates. Genomic sequencing results indicated that sLEP-B possessed only one intron. This is the first report of the loss of an intron in LEP-B in Perciformes. The different distribution patterns of sLEPs suggest different physiological roles of these two genes. The presence of HNF3β, a liver-enriched transcription factor, only in sLEP-A indicated abundant expression and metabolic function of sLEP-A in the liver. In an in vivo experiment, the expressions of brain sLEP-A and sLEP-B were observed to increase after a meal. During the short-term fasting, the expressions of sLEPs in mandarin fish brain were decreased significantly. A persistent and significant increase in hepatic sLEP-A expression supported a relationship between leptin and food intake in mandarin fish. These results suggest that sLEP-A plays an important role in the regulation of energy homeostasis in this carnivorous fish, and sLEP-B is probably a specialized gene responsible for the central nervous system (CNS) control of energy regulation.
    No preview · Article · Jan 2016 · Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
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    ABSTRACT: FXYD proteins regulate Na+/K+-ATPase (NKA), which is a primary active pump that provides the driving force that triggers osmoregulatory systems in teleosts. To explore the regulatory mechanisms between FXYD and NKA in euryhaline teleosts, the expression of NKA (mRNA, protein, and activity) and FXYD11 and their interaction were examined in the gills of brackish medaka (Oryzias dancena) when transferred from brackish water (BW; 15 ‰) to fresh water (FW) or seawater (SW; 35 ‰). The mRNA expression of Odfxyd11 and Odnka-α were elevated 48 h post-hypoosmotic transfer. Moreover, FXYD11 protein and NKA activity were upregulated 12 h after transfer to FW. When transferred to SW, the protein abundance of FXYD11 and the NKA α-subunit did not show apparent changes, while Odfxyd11 and Odnka-α mRNA expression and NKA activity increased significantly 12 h and 1 h post-transfer, respectively. To clarify the FXYD11 mechanisms involved in modulating NKA activity via their interaction, co-immunoprecipitation was further applied to O. dancena gills. The results revealed that the levels of protein–protein interaction between branchial NKA and FXYD11 increased acutely 12 h after the transfer from BW to FW. However, immediate upregulation of NKA activity 1 h following post-exposure to SW, without the elevation of protein–protein interaction levels, was found. Hence, branchial NKA activity of O. dancena was suggested to be rapidly regulated by FXYD11 interaction with NKA when acclimated to hypoosmotic environments. To the best of our knowledge, this is the first study that focuses on the efficacy of interactions between FXYD11 and NKA in the gills of euryhaline teleosts.
    No preview · Article · Jan 2016 · Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
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    ABSTRACT: Scent detection in an aquatic environment is dependent on the movement of water. We set out to determine the mechanisms for moving water through the olfactory organ of guitarfishes (Rhinobatidae, Chondrichthyes) with open nasal cavities. We found at least two. In the first mechanism, which we identified by observing dye movement in the nasal region of a life-sized physical model of the head of Rhinobatos lentiginosus mounted in a flume, olfactory flow is generated by the guitarfish's motion relative to water, e.g. when it swims. We suggest that the pressure difference responsible for motion-driven olfactory flow is caused by the guitarfish's nasal flaps, which create a region of high pressure at the incurrent nostril, and a region of low pressure in and behind the nasal cavity. Vortical structures in the nasal region associated with motion-driven flow may encourage passage of water through the nasal cavity and its sensory channels, and may also reduce the cost of swimming. The arrangement of vortical structures is reminiscent of aircraft wing vortices. In the second mechanism, which we identified by observing dye movement in the nasal regions of living specimens of Glaucostegus typus, the guitarfish's respiratory pump draws flow through the olfactory organ in a rhythmic (0.5–2 Hz), but continuous, fashion. Consequently, the respiratory pump will maintain olfactory flow whether the guitarfish is swimming or at rest. Based on our results, we propose a model for olfactory flow in guitarfishes with open nasal cavities, and suggest other neoselachians which this model might apply to.
    No preview · Article · Jan 2016 · Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
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    ABSTRACT: Some small mammals limit energy expenditure during winter conditions through torpor bouts, which are characterized by a decrease in body temperature and metabolic rate. Individuals arise periodically from torpor to restore critical functions requiring euthermia. Although most of the species involved do not feed during hibernation and rely on body reserves to fulfil energy requirements (fat-storing species), others hoard food in a burrow (food-caching species) and can feed during interbout euthermy. Whereas fat-storing species undergo a marked atrophy of the digestive tract, food-caching species have to maintain a functional digestive system during hibernation. Our study aimed to evaluate the absorption capacities of a food-caching species, the European hamster, throughout the annual cycle. In vivo intestinal perfusions were conducted in different groups of hamsters (n = 5) during the different life periods, namely before hibernation, in torpor, during interbout euthermy, and during summer rest. The triglyceride, non-esterified free fatty acid, starch, glucose and protein composition of the perfusate was evaluated before and after the 1 h perfusion of a closed intestinal loop. Triglyceride, starch and protein hydrolysis rates were similar in hibernating (torpid and euthermic) and non-hibernating hamsters. Intestinal absorption of free fatty acid was also similar in all groups. However, glucose uptake rate was higher during hibernation than during the summer. In contrast with fat-storing species, the intestinal absorption capacities of food-caching species are fully maintained during hibernation to optimize nutrient assimilation during short interbout euthermy. In particular, glucose uptake rate is increased during hibernation to restore glycaemia and ensure glucose-dependent pathways.
    No preview · Article · Jan 2016 · Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
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    ABSTRACT: Specific dynamic action (SDA), the increase in metabolic expenditure associated with consumption of a meal, represents a substantial portion of fish energy budgets and is highly influenced by ambient temperature. The effect of temperature on SDA has not been studied in yellowfin tuna (Thunnus albacares, Bonnaterre 1788), an active pelagic predator that occupies temperate and subtropical waters. The energetic cost and duration of SDA were calculated by comparing routine and post-prandial oxygen consumption rates. Mean routine metabolic rates in yellowfin tuna increased with temperature, from 136 mg O2 kg− 1 h− 1 at 20 °C to 211 mg O2 kg− 1 h− 1 at 24 °C. The mean duration of SDA decreased from 40.2 h at 20 °C to 33.1 h at 24 °C, while mean SDA coefficient, the percentage of energy in a meal that is consumed during digestion, increased from 5.9% at 20 °C to 12.7% at 24 °C. Digestion in yellowfin tuna is faster at a higher temperature but requires additional oxidative energy. Enhanced characterization of the role of temperature in SDA of yellowfin tuna deepens our understanding of tuna physiology and can help improve management of aquaculture and fisheries.
    No preview · Article · Jan 2016 · Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
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    ABSTRACT: The gut sac is a long-standing, widely used in vitro preparation for studying solute and water transport, and calculation of these fluxes requires an accurate assessment of volume. This is commonly determined gravimetrically by measuring the change in mass over time. While convenient this likely under-estimates actual net water flux (Jv) due to tissue edema. We evaluated whether the popular in vivo volume marker [14C]-PEG 4000, offers a more representative measure of Jv in vitro. We directly compared these two methods in five teleost species (toadfish, flounder, rainbow trout, killifish and tilapia). Net fluid absorption by the toadfish intestine based on PEG was significantly higher, by almost 4-fold, compared to gravimetric measurements, compatible with the latter under-estimating Jv. Despite this, PEG proved inconsistent for all of the other species frequently resulting in calculation of net secretion, in contrast to absorption seen gravimetrically. Such poor parallelism could not be explained by the absorption of [14C]-PEG (typically < 1%). We identified a number of factors impacting the effectiveness of PEG. One was adsorption to the surface of sample tubes. While it was possible to circumvent this using unlabelled PEG 4000, this had a deleterious effect on PEG-based Jv. We also found sequestration of PEG within the intestinal mucus. In conclusion, the short-comings associated with the accurate representation of Jv by gut sac preparations are not overcome by [14C]-PEG. The gravimetric method therefore remains the most reliable measure of Jv and we urge caution in the use of PEG as a volume marker.
    No preview · Article · Jan 2016 · Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
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    ABSTRACT: Medullary bone is a specialized bone found in the marrow cavity of laying birds. It provides a significant contribution to the calcium supply for egg shell formation. Medullary bone is distinguished from cortical bone by the presence of large amounts of a keratan sulfate proteoglycan (KSPG). The aims of the present experiment are to confirm the identity of the core protein of KSPG, identify a marker of medullary bone metabolism, and determine whether changes in keratan sulfate (KS) concentration in blood are associated with the egg-laying cycle. Using two different isolation techniques- one specific for bone and another for blood- we have identified bone sialoprotein (BSP) to be the core protein of this KSPG. We also determined that the amount of keratan sulfate (KS) in laying hen blood fluctuates in synchrony with the egg-laying cycle, and thus can serve as a specific marker for medullary bone metabolism. During the course of this investigation, we also found FGF-23 (phosphatonin) to be expressed in medullary bone, in synchrony with the egg-laying cycle. Western blotting was used to demonstrate the presence of this peptide in both laying hen blood and medullary bone extracts. The importance of FGF-23 (phosphatonin) and parathyroid hormone in normalizing the dramatic changes in plasma calcium and phosphorus during the 24 h egg-laying cycle is discussed.
    No preview · Article · Jan 2016 · Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
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    ABSTRACT: A complementary DNA (cDNA) that encodes the vitellogenin receptor (VgR) in the oriental river prawn, Macrobrachium nipponense, was cloned using expressed sequence tag analysis and a rapid amplification of cDNA ends approach. The coding region consists of 5920 base pairs (bp) that encode a 1902 amino acid protein, with a predicted molecular mass of 209 kDa. The coding region is flanked by a 45 bp 5ʹ-untranslated region (UTR) and a 166 bp 3ʹ-UTR. The deduced amino acid sequence of the M. nipponense VgR cDNA had typically conserved domains, such as an extracellular, lipoprotein-binding domain, epidermal growth factor-like and O-glycosylation domains, a transmembrane domain and a short C-terminal, cytosolic tail. Quantitative real-time PCR (qPCR) indicated that Mn-VgR is highly expressed in the female ovary. Expression analysis by qPCR demonstrated the larval and ovarian developmental stage-specific expression pattern. As the ovaries developed, the expression level of Mn-VgR gradually increased during the reproductive cycle (stage I), to reach a peak in stage III. Levels then dropped as a new development cycle was entered after reproduction molting. Eyestalk ablation led to a significant increase in the expression of Mn-VgR during the ovarian development stages (P < 0.05), when compared with the eyestalk-intact group. The investigation revealed that eyestalk ablation initially affected Mn-VgR expression and then influenced vitellogenesis. In adult females, VgR RNA interference (RNAi) dramatically delayed the maturation of the ovary, in accordance with the gonad somatic index. In addition, Mn-VgR RNAi led to vitellin depletion in the oocytes and the accumulation of vitellin in the hepatopancreas.
    No preview · Article · Jan 2016 · Comparative biochemistry and physiology. Part A, Molecular & integrative physiology