Electrophoresis (Electrophoresis)

Publisher: Electrophoresis Society; International Electrophoresis Society, Wiley-VCH Verlag

Journal description

Attracting high-quality papers from the world's leading laboratories ELECTROPHORESIS has proved itself indispensable in the life sciences where electrophoresis is the most widely used method. ELECTROPHORESIS is the foremost journal for new analytical and preparative methods and for innovative applications on all aspects of electrophoresis. It has an international scope and publishes original papers short communications and review articles in the following areas: biochemistry molecular and cell biochemistry genetics immunology microbiology clinical chemistry forensics food science and many more. Special Issues Paper Symposium issues devoted to new developments prepared by an expert Guest Editor are published regularly with special emphasis on capillary electrophoresis (2-3 issues per year)and proteomics. The journal also publishes proceedings of international and national electrophoresis meetings.

Current impact factor: 3.03

Impact Factor Rankings

2016 Impact Factor Available summer 2017
2014 / 2015 Impact Factor 3.028
2013 Impact Factor 3.161
2012 Impact Factor 3.261
2011 Impact Factor 3.303
2010 Impact Factor 3.569
2009 Impact Factor 3.077
2008 Impact Factor 3.509
2007 Impact Factor 3.609
2006 Impact Factor 4.101
2005 Impact Factor 3.85
2004 Impact Factor 3.743
2003 Impact Factor 4.04
2002 Impact Factor 4.325
2001 Impact Factor 4.282
2000 Impact Factor 3.385
1999 Impact Factor 3.447
1998 Impact Factor 3.054
1997 Impact Factor 2.848
1996 Impact Factor 2.467
1995 Impact Factor 2.73
1994 Impact Factor 2.274
1993 Impact Factor 1.842
1992 Impact Factor 2.159

Impact factor over time

Impact factor

Additional details

5-year impact 2.72
Cited half-life 7.90
Immediacy index 0.53
Eigenfactor 0.02
Article influence 0.58
Website Electrophoresis website
Other titles Electrophoresis (Online), Electrophoresis, Proteomics reviews
ISSN 1522-2683
OCLC 43388561
Material type Periodical, Program, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details

Wiley-VCH Verlag

  • Pre-print
    • Author cannot archive a pre-print version
  • Post-print
    • Author cannot archive a post-print version
  • Restrictions
    • Upon funder agreement with publisher
  • Conditions
    • Pre-print may be deposited on personal intranet or institutional intranet repository, but not on a public repository
    • Pre-print must not updates with future versions
    • Published source must be acknowledged with set phrases (See policy)
    • Must link to publisher's site: http://www.interscience.wiley.com/
    • Publisher's version/PDF cannot be used
    • Some journal exceptions-check individual homepages
  • Classification

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: The current trend for increasing technical complexity in the field of CE-ESI-MS interfaces has incited for more accessible alternatives. In this work, a simple low sheath-flow ESI interface operating in the sub-microliter nanospray regime without nebulizing gas assistance was evaluated. The use of sheath liquid enabled improving the ionization of the analytes, while the absence of nebulizing gas minimized sample dilution and loss of efficiency. After a rapid qualification, the effect of main operational parameters such as sheath liquid composition and flow rate, working distance, ESI potential was studied. Simulation of the mixing processes inside the Taylor cone proved its size to be of utmost importance in band broadening processes. As a proof of concept, the interface was eventually applied to a set of representative basic drugs analysed by CE-TOF/MS. Limits of detection reached the 25-100 ppb range with suitable robustness and repeatability results. This design has demonstrated good performance while being simple and accessible to the user. This article is protected by copyright. All rights reserved.
    No preview · Article · Feb 2016 · Electrophoresis
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    ABSTRACT: In this paper, an on-line immunoaffinity solid-phase extraction capillary electrophoresis mass spectrometry (IA-SPE-CE-MS) method using magnetic beads (MBs) is described for the analysis of serum transthyretin (TTR), which is a protein related to different types of amyloidosis. First, purification of TTR from serum was investigated by off-line immunoprecipitation and CE-MS. The suitability of three Protein A (ProA) MBs (Protein A Ultrarapid Agarose(TM) (UAPA), Dynabeads(®) Protein A (DyPA) and SiMAG-Protein A (SiPA)) and AffiAmino Ultrarapid Agarose(TM) (UAAF) MBs to prepare an IA sorbent with a polyclonal antibody (Ab) against TTR, was studied. In all cases results were repeatable and it was possible the identification and the quantitation of the relative abundance of the 6 most abundant TTR proteoforms. Although recoveries were the best with UAPA MBs, UAAF MBs were preferred for on-line immunopurification because Ab was not eluted from the MBs. Under the optimised conditions with standards in IA-SPE-CE-MS, microcartridge lifetime (>20 analyses/day) and repeatability (2.9 and 4.3 % RSD for migration times and peak areas) were good, the method was linear between 5- 25 μg·mL(-1) and limit of detection (LOD) was around 1 μg·mL(-1) (25 times lower than by CE-MS, ≈25 μg·mL(-1) ). A simple off-line sample pretreatment based on precipitation of the most abundant proteins with 5% (v/v) of phenol was necessary to clean-up serum samples. The potential of the on-line method to screen for familial amyloidotic polyneuropathy type I (FAP-I), which is the most common hereditary systemic amyloidosis, was demonstrated analysing serum samples from healthy controls and FAP-I patients. This article is protected by copyright. All rights reserved.
    No preview · Article · Feb 2016 · Electrophoresis
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    ABSTRACT: The critical micellar concentration (CMC) is one of the fundamental characteristics of surfactants and its determination is crucial for detail understanding of micelles formation. In this study the critical micellar concentration of sodium dodecyl sulfate (SDS) in presence of acetonitrile was determined by two independent experimental techniques, capillary electrophoresis and fluorescence correlation spectroscopy. Yet, studies of SDS micellization in solutions containing acetonitrile as organic modifier are sparse and inconsistent in literature. The measurements were performed for various acetonitrile contents in the range 0 - 50% v/v. At acetonitrile contents of up to 10% v/v the CMC is lower when compared to the aqueous solution, while increasing acetonitrile content causes a significant increase of the CMC. Formation of micelles was observed up to acetonitrile concentrations of 35% v/v, which is in contrary to the most reports in literature. Based on the results of the fluorescence correlation spectroscopy experiments, we were able to confirm that presence of acetonitrile causes a gradual increase of the size of the micelles with increasing concentration of SDS. Simultaneously, we proved that the classical conductivity approach for the determination of the critical micellar concentration does not yield reliable results in the presence of higher content of an organic modifier such asacetonitrile. This article is protected by copyright. All rights reserved.
    No preview · Article · Feb 2016 · Electrophoresis
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    ABSTRACT: A number of studies have demonstrated a strong association between the antioxidant properties of rosemary polyphenols and their chemoprotective activity. However, the prooxidant effects of rosemary polyphenols have been rarely reported. In this work, a foodomics study is performed to investigate the in vitro autooxidation of carnosic acid (CA), carnosol (CS) and a polyphenol-enriched rosemary extract (SC-RE) in cell culture conditions. The results revealed that rosemary polyphenols autooxidation in culture medium generated H2 O2 at different rates. Generated H2 O2 levels by SC-RE and CA, but not CS, were correlated with intracellular reactive oxygen species (ROS) generation in HT-29 cells, and were partially involved in their anti-proliferative effect in this cell line. These compounds also induced different effects on glutathione metabolism. Results also indicated that high extracellular H2 O2 concentrations, resulting of using high (45 μg/mL) SC-RE concentration in culture media, exerted some artifactual effects related with cell cycle, but they did not influence the expression of relevant molecular biomarkers of stress. This article is protected by copyright. All rights reserved.
    No preview · Article · Feb 2016 · Electrophoresis
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    ABSTRACT: The influence of a high electric field applied on both fluid flow and particle velocities is quantified at large Peclet numbers. The experiments involved simultaneous Particle Image Velocimetry (PIV) and flow rate measurements. These are conducted in polydimethylsiloxane (PDMS) channels with spherical non conducting polystyrene particles and deionized water as the background flow. The high electric field tests produced up to three orders of magnitude higher electrokinetic velocities than any previous reports. The maximum electroosmotic velocity and electrophoretic velocity measured were 3.55 m/sec and 2.3 m/s. Electrophoretic velocities are measured over the range of 100 V/cm < E < 250,000 V/cm. The results are separated according to the different nonlinear theoretical models including low and high Peclet numbers, and weak and strong concentration polarization. They show good agreement with the models. Such fast velocities could be used for flow separation, mixing, transport, control and manipulation of suspended particles as well as micro-thrust generation among other applications. This article is protected by copyright. All rights reserved.
    No preview · Article · Feb 2016 · Electrophoresis
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    ABSTRACT: Graphene oxide (GO) has been considered as a promising stationary phase for chromatographic separation. However, the very strong adsorption of the analytes on the GO surface lead to the severe peak tailing, which in turn resulting in decreased separation performance. In this work, GO and silica nanoparticles hybrid nanostructures (GO/SiO2 NPs@column) were coated onto the capillary inner wall by passing the mixture of GO and silica sol through the capillary column. The successful of coating of GO/SiO2 NPs onto the capillary wall was confirmed by scanning electron microscopy and electroosmotic flow mobilities test. By partially covering the GO surface with silica nanoparticles, the peak tailing was decreased greatly while the unique high shape selectivity arises from the surface of remained GO was kept. Consequently, compared with the column modified with GO (GO@column), the column modified with GO and silica nanoparticles through layer-by-layer method (GO-SiO2 NPs@column), or the column modified with silica nanoparticles (SiO2 NPs@column), GO/SiO2 NPs@column possessed highest resolutions. The GO/SiO2 NPs@column was applied to separate egg white and both acidic and basic proteins as well as three glycoisoforms of ovalbumin were separated in a single run within 36 min. The intra-day, inter-day, and column-to-column reproducibilities were evaluated by calculating the relative standard deviations (RSDs) of the retention of naphthalene and biphenyl in OT-CEC. The RSD values were found to be less than 7.1%. This article is protected by copyright. All rights reserved.
    No preview · Article · Feb 2016 · Electrophoresis
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    ABSTRACT: Glycosylation is a posttranslational modification that occurs during production of many protein-based biologic drugs and can have a profound impact on their biological, clinical and pharmacological properties. Quality by design (QbD), process optimization, and advance in manufacturing technology create a demand for robust, sensitive, and accurate profiling and quantification of antibody glycosylation. Potential drawbacks in antibody glycosylation profiling include the high hands-on time required for sample preparation and several hours for data acquisition and analysis. Rapid and high-throughput N-glycan profiling and characterization along with automation for sample preparation and analysis are essential for extensive antibody glycosylation analysis due to the substantial improvement of turnaround time. The first part of this review article will focus on the recent progress in rapid and high-throughput sample preparation and analysis of antibody glycosylation. Subsequently, the article will cover a brief overview of various separation and mass spectrometric methods for the rapid and high-throughput (HTP) analysis of N-glycans in antibodies. Finally, we will discuss the recent developments in process analytical technologies (PAT) for the screening and quantification of N-glycans in antibodies. This article is protected by copyright. All rights reserved.
    No preview · Article · Feb 2016 · Electrophoresis
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    ABSTRACT: A CE-ESI-MS method was developed and validated for the separation and quantitative analysis of amino acids in urine. Experimental parameters related to the CE-MS interface, background electrolyte (BGE) and mass spectrometer (MS) settings were optimized providing a good separation of 27 amino acids, including the isomers L-leucine, L-isoleucine and L-alloisoleucine, in less than 30 min. The sheath liquid was composed by 0.50% formic acid in 60% (v,v) methanol-water delivered at a flow rate of 5 μL/min. The BGE consisted of 0.80 mol/L formic acid at pH 1.96 and 15% methanol. A pH stacking procedure was implemented to enhance sensitivity (a 12.5% NH4OH solution was injected at 0.5 psi/9 s prior to samples injected at 0.6 psi/20 s). The proposed method was validated according to FDA and ICH protocols exhibiting acceptable parameters. Analytical curves presented coefficients of determination from 0.996 to 0.9997 (with large F statistics and low p-values). Limits of detection and quantification ranged from 0.63 to 29 μmol/L and from 1.9 to 86 μmol/L, respectively. Practical repeatability was obtained for all AA with coefficients of variation better than 0.55 %CV (migration time) and 1.7 %CV (peak area ratios; methionine sulfone as internal standard). Recoveries of AA in spiked urine ranged from 92.0 to 123% with few exceptions. Moreover, a successful quantification of amino acids in pooled control and test urine samples, which compose a VUR cohort, was achieved showing the potential applicability of the proposed method for targeted metabolomics studies using CE-ESI-MS with an Ion Trap as mass analyzer. This article is protected by copyright. All rights reserved
    No preview · Article · Jan 2016 · Electrophoresis
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    ABSTRACT: Iron is an essential micronutrient for all marine organisms, but it is also a growth limiting factor as the iron concentrations in the open ocean are below 1 nmol L(-1) . At high chloride concentrations present in sea water, iron is almost entirely bound to organic ligands of the dissolved organic matter fraction, which are mostly of unknown structure. The input from rivers was traditionally considered as less important due to estuarine sedimentation processes of the mainly colloidal iron particles. However, recent studies have shown that this removal is not complete and riverine input may represent an important iron source in the open ocean. In this context, iron transport by land-derived natural organic matter (NOM), and dissolved organic matter (DOM) have been identified as carrier mechanisms for riverine iron. The aim of this work is to characterize complexes containing iron and other metals in waters simulating estuarine conditions in order to help understand which role iron-DOM compounds play in the open ocean. A method based on size-exclusion chromatography (SEC) with sequential UV/VIS and ICP-MS detection was developed for investigation of DOM size distribution and for assessment of the size-dependent metal distribution in NOM-rich surface water. Furthermore, sample matrix experiments were also performed revealing a dependence of DOM size distribution upon seawater concentration and different compounds present in seawater. Finally, efforts toward determination of DOM size with standardization with typical SEC standards indicate that only relative comparisons are possible with this approach, and that the sample matrix composition strongly influences obtained results. This article is protected by copyright. All rights reserved.
    No preview · Article · Jan 2016 · Electrophoresis
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    ABSTRACT: This work explores the use of advanced mass spectrometry imaging (IMS) and magnetic resonance imaging (MRI) techniques in food science and nutrition to evaluate food sensory characteristics, nutritional value and health benefits. Determining the chemical content and applying imaging tools to food metabolomics offers detailed information about food quality, safety, processing, storage, and authenticity assessment. IMS and MRI are powerful analytical systems with an excellent capability for mapping the distribution of many molecules, and recent advances in these platforms are reviewed and discussed, showing the great potential of these techniques for small molecule-based food metabolomics research. This article is protected by copyright. All rights reserved.
    No preview · Article · Jan 2016 · Electrophoresis
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    ABSTRACT: Here we have reviewed separation studies utilizing monolithic capillary columns for separation of compounds preceding MS analysis. The review is divided in two parts according to the used separation method, namely CEC and capillary LC (cLC). Based on our overview, monolithic CEC-MS techniques have been more focused on the syntheses of highly specialized and selective separation phase materials for fast and efficient separation of specific types of analytes. In contrast, monolithic cLC-MS is more widely used and is often employed, for instance, in the analysis of oligonucleotides, metabolites, and peptides and proteins in proteomic studies. While poly(styrene-divinylbenzene)-based and silica-based monolithic capillaries found their place in proteomic analyses, the other laboratory-synthesized monoliths still wait for their wider utilization in routine analyses. The development of new monolithic materials will most likely continue due to the demand of more efficient and rapid separation of increasingly complex samples. This article is protected by copyright. All rights reserved.
    No preview · Article · Jan 2016 · Electrophoresis
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    ABSTRACT: Capillary electrophoresis gets more and more important in many fields of bioanalytical chemistry. Besides optical detection, hyphenation to ESI-MS detection is increasingly applied for sensitive identification purposes. Unfortunately, many CE techniques and methods established in research and industry are not compatible to ESI-MS since essential components of the background electrolyte interfere in ES ionization. In order to identify unknown peaks in established CE methods, here, a heart-cut 2D-CE separation system is introduced using a fully isolated mechanical valve with an internal loop of only 20 nL. In this system, the sample is separated using potentially any non-ESI-compatible method in the first separation dimension. Subsequently, the portion of interest is cut by the internal sample loop of the valve and reintroduced to the second dimension where the interfering compounds are removed, followed by ESI-MS detection. When comparing the separation efficiency of the system with the valve to a system using a continuous capillary only a slight increase in peak width is observed. Ultra violet/visible (UV/Vis) detection is integrated in the first dimension for switching time determination, enabling reproducible cutting of peaks of interest. The feasibility of the system is successfully demonstrated by a 2D analysis of a BSA tryptic digest sample using a non-volatile (phosphate based) background electrolyte in the first dimension. This article is protected by copyright. All rights reserved.
    No preview · Article · Jan 2016 · Electrophoresis
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    ABSTRACT: In the present study, for the first time electromembrane extraction (EME) followed by high performance liquid chromatography (HPLC) coupled with ultraviolet (UV) detection was optimized and validated for quantification of four Gn-RH agonist anticancer peptides (alarelin, leuprolide, buserelin and triptorelin) in biological and aqueous samples. The parameters influencing electromigration were investigated and optimized. The membrane consists 95% of 1-octanol and 5% di-(2-ethylhexyl) phosphate (DEHP) immobilized in the pores of a hollow fiber. A 20 V electrical field was applied to make the analytes migrate from sample solution with pH 7.0, through the supported liquid membrane (SLM) into an acidic acceptor solution with pH 1.0 which was located inside the lumen of hollow fiber. Extraction recoveries in the range of 49 and 71% within 15 min extraction time were obtained in different biological matrices which resulted in preconcentration factors in the range of 82-118 and satisfactory repeatability (7.1 < RSD% < 19.8). The method offers good linearity (2.0-1000 ng mL(-1) ) with estimation of regression coefficient higher than 0.998. The procedure allows very low detection and quantitation limits of 0.2 and 0.6 ng mL(-1) , respectively. Finally, it was applied to determination and quantification of peptides in human plasma and wastewater samples and satisfactory results were yielded. This article is protected by copyright. All rights reserved.
    No preview · Article · Jan 2016 · Electrophoresis
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    ABSTRACT: In this paper, we report on a novel electrophoretic separation and analysis method for membrane pore forming proteins in multilayer lipid membranes (MLMs) in order to overcome the problems related to current separation and analysis methods of membrane proteins, and to obtain a high-performance separation method on the basis of specific properties of the lipid membranes. We constructed MLMs, and subsequently characterized membrane pore forming protein behavior in MLMs. Through the use of these MLMs, we were able to successfully separate and analyze membrane pore forming proteins in MLMs. To the best of our knowledge, this research is the first example of membrane pore forming protein separation in lipid membranes. Our method can be expected to be applied for the separation and analysis of other membrane proteins including intrinsic membrane proteins and result in high-performance by utilizing the specific properties of lipid membranes. This article is protected by copyright. All rights reserved.
    No preview · Article · Jan 2016 · Electrophoresis
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    ABSTRACT: Sugar based ionic surfactants forming micelles are known to suppress electrospray ionization of various compounds due to decrease in surface tension upon micelle formation [1]. For the first time, poly (sodium N-alkenyl-α-D-glucopyranoside, (poly-α-D-SUGP) based surfactants with different chain lengths and head groups have been successfully synthesized, characterized and applied as compatible chiral selector for MEKC-ESI-MS/MS. First, the effect of polymerization concentration of the monomer, sodium N-undecylenyl-α-D-glucopyranoside 4,6-hydrogen phosphate (α-D-SUGP), was evaluated by enantioseparation of one anionic compound [1,1'-binaphthyl-2,2'diyl-hydrogen phosphate (BNP)] and one zwitterionic compound (dansylated phenylalanine) in MEKC-UV to find the optimum molar surfactant concentration for polymerization. Next, MEKC-UV and MEKC-MS was compared for the enantioseparation of BNP. The influence of polymeric glucopyranoside-based surfactant head groups and carbon chain lengths on chiral Rs was evaluated for two classes of cationic drugs (ephedrine alkaloids and β-blockers). Finally, enantioselective MEKC-MS of ephedrine alkaloids and β-blockers were profiled at their optimum pH 5.0 and 7.0, respectively using 20 mM NH4 OAc, 25 mM poly-α-D-SUGP at 30 kV and 25 °C under optimum spray chamber conditions. The LOD for most of the enantiomers ranges from 10 ng/mL-100 ng/ml with S/N of at least ≥ 3.0. This article is protected by copyright. All rights reserved.
    No preview · Article · Jan 2016 · Electrophoresis