Journal of Microbiology and Biotechnology (J Microbiol Biotechnol)

Publisher: Hanʼguk Sanŏp Misaengmul Hakhoe

Current impact factor: 1.53

Impact Factor Rankings

2016 Impact Factor Available summer 2017
2014 / 2015 Impact Factor 1.525
2013 Impact Factor 1.32
2012 Impact Factor 1.399
2011 Impact Factor 1.381
2010 Impact Factor 1.224
2008 Impact Factor 2.062
2007 Impact Factor 2.062
2006 Impact Factor 2.037
2005 Impact Factor 1.744
2004 Impact Factor 1.663
2003 Impact Factor 1.202
2002 Impact Factor 1.364
2001 Impact Factor 1.338
2000 Impact Factor 1.083
1999 Impact Factor 0.809
1998 Impact Factor 0.436
1997 Impact Factor 0.226

Impact factor over time

Impact factor
Year

Additional details

5-year impact 1.54
Cited half-life 5.50
Immediacy index 0.22
Eigenfactor 0.01
Article influence 0.37
Website Journal of Microbiology and Biotechnology website
ISSN 1017-7825
OCLC 261226927
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publications in this journal


  • No preview · Article · Dec 2015 · Journal of Microbiology and Biotechnology
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    ABSTRACT: In this study, the prevalence of Shiga toxin-producing Escherichia coli (STEC) was investigated among raw meat or meat products from slaughterhouses and retail markets in South Korea, and their potential for antibiotic resistance and virulence was further analyzed. A total of 912 raw meats, including beef, pork, and chicken, were collected from 2008 to 2009. E. coli strains were frequently isolated in chicken meats (176/233, 75.9%), beef (102/217, 42.3%), and pork (109/235, 39.2%). Putative STEC isolates were further categorized, based on the presence or absence of the Shiga toxin (stx) genes, followed by standard O-serotyping. Polymerase chain reaction assays were used to detect the previously defined virulence genes in STEC, including Shiga toxins 1 and Shiga toxin 2 (stx1 and 2), enterohemolysin (ehxA), intimin (eaeA), STEC autoagglutination adhesion (saa), and subtilase cytotoxin (subAB). All carried both stx1 and eae genes, but none of them had the stx2, saa, or subAB genes. Six (50.0%) STEC isolates possessed the ehxA gene, which is known to be encoded by the 60-megadalton virulence plasmid. Our antibiogram profiling demonstrated that some STEC strains, particularly pork and chicken isolates, displayed a multiple drug-resistance phenotype. RPLA analysis revealed that all the stx1-positive STEC isolates produced Stx1 only at the undetectable level. Altogether, these results imply that the locus of enterocyte and effacement (LEE)-positive strains STEC are predominant among raw meats or meat products from slaughterhouses or retail markets in Korea. © 2015 by The Korean Society for Microbiology and Biotechnology.
    No preview · Article · Sep 2015 · Journal of Microbiology and Biotechnology
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    ABSTRACT: The aim of this study was to develop a SYBR Green-based real-time PCR assay for the rapid, specific, and sensitive detection of Acidovorax avenae subsp. citrulli, which causes bacterial fruit blotch (BFB), a serious disease of cucurbit plants. The molecular and serological methods currently available for the detection of this pathogen are insufficiently sensitive and specific. Thus, a novel SYBR Green-based real-time PCR assay targeting the YD-repeat protein gene of A. avenae subsp. citrulli was developed. The specificity of the primer set was evaluated using DNA purified from 6 isolates of A. avenae subsp. citrulli, 7 other Acidovorax species, and 22 of non-targeted strains, including pathogens and non-pathogens. The AC158F/R primer set amplified a single band of the expected size from genomic DNA obtained from the A. avenae subsp. citrulli strains but not from the genomic DNA of other Acidovorax species, including that of other bacterial genera. Using this assay, it was possible to detect at least one genomeequivalents of the cloned amplified target DNA using 5 × 100 fg/μl of purified genomic DNA per reaction or using a calibrated cell suspension, with 6.5 colony-forming units per reaction being employed. In addition, this assay is a highly sensitive and reliable method for identifying and quantifying the target pathogen in infected samples that does not require DNA extraction. Therefore, we suggest that this approach is suitable for the rapid and efficient diagnosis of A. avenae subsp. citrulli contaminations of seed lots and plants. ©2015 by The Korean Society for Microbiology and Biotechnology.
    No preview · Article · Sep 2015 · Journal of Microbiology and Biotechnology
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    ABSTRACT: The purpose of this study was to investigate lactic acid bacteria with antioxidative and probiotic activities isolated from Korean healthy infant feces and kimchi. Isolates A1, A2, S1, S2, and S3 were assigned to Lactobacillus sp. and isolates A3, A4, E1, E2, E3, and E4 were assigned to Leuconostoc sp. on the basis of their physiological properties and 16S ribosomal DNA sequence analysis. Most strains were confirmed as safe bioresources through nonhemolytic activities and non-production of harmful enzymes such as β-glucosidase, β- glucuronidase and tryptophanase. The 11 isolates showed different resistance to acid and bile acids. In addition, they exhibited antibacterial activity against foodborne bacteria, especially Bacillus cereus, Listeria monocytogenes, and Escherichia coli. Furthermore, all strains showed significantly high levels of hydrophobicity. The antioxidant effects of culture filtrates of the 11 strains included 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging capacity, 2.2’- azino-bis (2-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity, and superoxide dismutase activity. The results revealed that most of the culture filtrates have effective scavenging activity for DPPH and ABTS radicals. All strains appeared to have effective superoxide dismutase activity. In conclusion, the isolated strains A1, A3, S1, and S3 have significant probiotic activities applicable to the development of functional foods and health-related products. These strains might also contribute to preventing and controlling several diseases associated with oxidative stress, when used as probiotics. © 2015 by The Korean Society for Microbiology and Biotechnology.
    No preview · Article · Sep 2015 · Journal of Microbiology and Biotechnology
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    ABSTRACT: A total of 646 strains, including green algae and diatoms, were isolated from 220 samples to screen microalgae with high lipid productivity (LP). The samples were obtained from nine habitats in Northern Xinjiang, China in June 2013. This study initially identified eight lipidrich strains, namely, Desmodesmus intermedius XJ-498, D. intermedius XJ-145, D. intermedius XJ- 99, Monoraphidium pusillum XJ-489, M. dybowskii XJ-435, M. dybowskii XJ-151, Mychonastes homosphaera XJ-488, and Podohedriella falcata XJ-176, based on 18S rDNA sequencing. The strains were cultured in a photobioreactor for the same period. Results showed that the specific growth rate (day-1) of M. pusillum XJ-489 was the highest (1.14 ± 0.06), and the biomass concentration (g/l) of D. intermedius XJ-99 was the highest (2.84 ± 0.3). Futhermore, the lipid content (%) of M. dybowskii XJ-151 was the highest (33.5 ± 4.38), and the lipid productivity (mg l-1 day-1) of My. homosphaera XJ-488 was the highest (86.41 ± 9.04). C16 to C18 accounted for 86% to 98% of the total lipid, and the biodiesel qualities of the selected algae corresponded to international standards. This study suggests that My. homosphaera XJ-488, D. intermedius XJ-99, and M. dybowskii XJ-151 are the most potential strains for biodiesel production among all the isolated strains. © 2015 by The Korean Society for Microbiology and Biotechnology.
    No preview · Article · Jun 2015 · Journal of Microbiology and Biotechnology
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    ABSTRACT: Rhizospheric zone abutting plant roots usually clutches a wealth of microbes. In recent past, enormous genetic resources have been excavated with potential applications in host plant interaction and ancillary aspects. Two Pseudomonads were isolated and identified through 16S rRNA and rpoD sequence analyses as P. fluorescens QAU-67 and P. putida QAU-90. Initial biochemical characterization and their root-colonizing traits indicated their potential role in plant growth promotion. Some such aerobic systems, involved in gluconic acid production and phosphate solubilization, essentially require the PQQ dependent glucose dehydrogenase (GDH) in the genome. The PCR screening and amplification of GDH and PQQ and subsequent induction of mutagenesis characterized for their possible role in growth promotion was probed in vitro in lettuce while in vivo in rice, bean and tomato plants. The results showed significant differences (p≤0.05) in parameters such as: plant height, fresh weight and dry weight etc., deciphering a clear and in fact complementary role of GDH and PQQ in plant growth promotion. Our study not only provides direct evidence on the in vivo role of GDH and PQQ in host plants but also reveals their functional inadequacy in the event of mutation at either of these loci.
    No preview · Article · May 2015 · Journal of Microbiology and Biotechnology
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    ABSTRACT: The production of gamma-aminobutyric acid (GABA) using GABA-producing lactic acid bacteria (LAB) has been considered to be an attractive strategy. However, some LAB may produce biogenic amines (BA), which may be of concern from the safety viewpoint. The aim of the present study was to characterize the production of GABA and BA in the soybean pastes fermented by Aspergillus oryzae (A. oryzae) FMB S46471 and GABA-producing Lactobacillus brevis (L. brevis) GABA 100. After a ripening period of 90 days, the levels of BA (putrescine, cadaverine, histamine, and tyramine) and GABA in the fermented soybean were assessed by highperformance liquid chromatography. The soybean pastes fermented by A. oryzae and L. brevis showed a range of 7,130-11,592 mg/kg for GABA, 178-305 mg/kg for tyramine, 139-163 mg/kg for putrescine, 7.4-10.8 mg/kg for histamine, and 7.1-7.9 mg/kg for cadaverine, whereas the soybean pastes fermented by A. oryzae only showed a range of 30-1,671 mg/kg for GABA, 0.8-189 mg/kg for tyramine, 1.3-85 mg/kg for putrescine, up to 3.6 mg/kg for histamine, and 0.2-2.4 mg/kg for cadaverine. The results showed that the production of GABA was accompanied by the increase in the production of BA, even though the production levels of histamine and cadaverine were very low. This is the first study to simultaneously characterize the production of BA and GABA in GABA-enriched fermented soybean pastes, and warrants further study to minimize the production of BA while optimizing the production of GABA. © 2015 by The Korean Society for Microbiology and Biotechnology.
    No preview · Article · Apr 2015 · Journal of Microbiology and Biotechnology
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    ABSTRACT: Tuberculosis (TB) is an infectious disease transmitted by aerosol droplets characterized by forming granulomatous lesions. Although the number of people infected in the population is high, the vast majority does not exhibit symptoms of active disease and only 5 - 10% develops the disease after a latent period that can vary from weeks to years. The bases of the immune response for this resistance are unknown, but it depends on a complex interaction between the environment, the agent and the host. The analysis of cellular components of M. tuberculosis shows important host-pathogen interactions, metabolic pathways, virulence mechanisms and mechanisms of adaptation to the environment. However the M. tuberculosis proteome still remains largely uncharacterized in terms of virulence and pathogenesis. Here we summarize some of the major proteomic studies performed to scrutinize all the mycobacterial components.
    No preview · Article · Mar 2015 · Journal of Microbiology and Biotechnology
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    ABSTRACT: The well-characterized gram-positive bacterium Bacillus subtilis is an outstanding industrial candidate for protein expression due to its single membrane and high capacity of secretion simplifying downstream processing of secretory proteins. During the last years, there has been continuous progress in the illustration of secretion mechanisms and application of this robust host in various fields of Life Science, such as enzyme production, feed additives, food and pharmaceutical industries. Here, we review the developments of Bacillus subtilis as highly promising expression system illuminating strong, chemical- and temperature-inducible and other types of promoters, strategies for ribosome-binding-site utilization and the novel approach of signal peptide selection. Furthermore, we will outline main steps of the Sec pathway and the relevant elements as well as their interactions. In addition, we will introduce the latest discoveries of Tat related complexes' structures and functions and the countless applications of this full-folded protein secretion pathway. This review also lists some of the current understandings of ATP-binding cassette transporters. According to the extensive knowledge on the genetic modification strategies and molecular biology of Bacillus subtilis, we propose some suggestions and strategies improving the yield of intended productions. We expect it to promote strikingly future developments in the optimization and application of this bacterium.
    No preview · Article · Mar 2015 · Journal of Microbiology and Biotechnology
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    ABSTRACT: The endo-polygalacturonase gene (endo-pgaA) was cloned from DNA of Aspergillus niger SC323 using the cDNA synthesized by overlapping PCR, and successfully expressed in Saccharomyces cerevisiae EBY100 through fusing α-factor signal peptide of yeast. The full-length cDNA consists of 1113 bp and encodes a protein of 370 amino acids with a calculated molecular mass of 38.8 kDa. After induction by galactose for 48 h, the activity of recombinant endo-PgaA in culture supernatant can reach up to 1448.48 U/mg. The recombinant protein was purified to homogeneity by ammonium sulfate precipitation and gel filtration column chromatography and subsequently characterized. The optimal pH and temperature of the purified recombinant enzyme were 5.0 and 50 °C, respectively. The Michaelis-Menten constant (Km) and maximal velocity (Vmax) of the enzyme for pectin were 88.54 μmol/ml and 175.44 μmol/mg/min, respectively. The enzyme activity was enhanced by Ca(2+), Cu(2+), Na(+) and strongly inhibited by Pb(2+), Mn(2+). The pectin hydrolysates were mainly galacturonic acid and other oligo-galacturonates. Therefore, these characteristics suggest that the recombinant endo-PgaA may be of potential use in food and feed industry.
    No preview · Article · Mar 2015 · Journal of Microbiology and Biotechnology
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    ABSTRACT: Recently, we isolated HY253, a novel decahydrofluorene analog, with the molecular structure of 7,8a-divinyl-2,4a,4b,5,6,7,8,8a,9,9a-decahydro-1H-fluorene-2,4a,4b,9a-tetraol from the roots of Aralia continentalis, which is called Dokwhal, the traditional medicinal herb. Also, we reported previously its cytotoxic activity on cancer cell proliferation in human lung cancer A549 and cervical cancer HeLa cells. The current study was aimed to evaluate its detailed molecular mechanisms on cell cycle arrest and apoptotic induction in human hepatocellular carcinoma HepG2 cells. A flow cytometric analysis of HepG2 cells treated with 60 micrometer HY253 revealed appreciable cell cycle arrest at the G1 phase via inhibition of Rb phosphorylation and down-regulation of cyclin D1. Furthermore, using western blots, up-regulation of cyclin-dependent kinase inhibitors, such as p21(CIP1) and p27(KIP1), was associated with this G1 phase arrest. Moreover, TUNEL assay and immunoblottings revealed apoptotic induction in HepG2 cells treated with 100 micrometer HY253 for 24 h, which is associated with cytochrome c release from mitochondria, via down-regulation of anti-apoptotic Bcl-2 protein which in turn resulted in activation of caspase-9 and -3, and proteolytic cleavage of poly(ADP-ribose) polymerase (PARP). Accordingly, we suggest that HY253 may be a potent chemotherapeutic hit compound for treating human liver cancer cells via up-regulation and activation of p53 gene.
    No preview · Article · Feb 2015 · Journal of Microbiology and Biotechnology
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    ABSTRACT: Cadaverine (1,5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, there are not many reports direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined using cadA over-expressed Escherichia coli (E. coli) strain and various parameters were optimized for the whole-cell biocatalyst at high substrate concentrations resulting in pyridoxal-5'-phosphate (PLP) is critical factor for biotransformation. When 0.025mM PLP and 1.75M lysine in 500mM sodium acetate buffer were used (pH6), 91% of lysine consumption and about 80% conversion to cadaverine was successfully achieved.
    No preview · Article · Feb 2015 · Journal of Microbiology and Biotechnology