Shanghai kou qiang yi xue = Shanghai journal of stomatology

Current impact factor: 0.00

Impact Factor Rankings

Additional details

5-year impact 0.00
Cited half-life 0.00
Immediacy index 0.00
Eigenfactor 0.00
Article influence 0.00
Website Shanghai Journal of Stomatology / Shang Hai Kou Qiang Yi Xue / 上海口腔醫學 website
ISSN 1006-7248

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To detect the expression of Wnt5a in lesions of chronic apical periodontitis and determine the relationship between expression of Wnt5a and inflammation degree. Methods: Ten patients with chronic apical periodontitis and 5 healthy controls were enrolled in this study. According to the inflammatory cell infiltration, the specimens were divided into 2 groups: severe inflammation group and mild inflammation group. The expression of Wnt5a was measured by real-time PCR (RT-PCR) and immunohistochemical analysis in the lesions of chronic apical periodontitis. The amount of Wnt5 expression was assayed and compared in different inflammation levels. Statistical analysis was performed using SPSS 13.0 software package. Results: Wnt5a was detected in both groups. Expression of Wnt5a mRNA in patients were significantly higher than the controls (P<0.05). According to inflammation level, the positive expression rate of Wnt5a in severe inflammation group was significantly higher than the controls (P<0.01), and Wnt5a positive expression in mild inflammation group was also significantly higher than the controls (P<0.05). The expression of Wnt5a was significantly different between severe inflammation group and mild inflammation group (P<0.05). Conclusions: The expression of Wnt5a increases as the severity of tissue inflammation increases, which indicates that Wnt5a plays an important role in the development of chronic apical periodontitis.
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To prepare chitosan/β-glycerophosphate salt (CS/β-GP) thermosensitive hydrogel membranes loaded enamel matrix proteins and detect the guided bone regeneration properties. Methods: A newly membrane was synthesized using thermal phase inversion property of the CS/ββ-GP system. The membrane was synthesized and added with protein BSA. The concentration of protein was detected at different time points by enhanced protein assay kit, and the protein release curve was drawn. CS/β-GP membrane added EMPs (1.0 g) as group A, CS/β-GP (1.0 g) membrane as group B and nothing as blank control group (group C). They were co-cultured with ST2 cells. The mechanical properties of the membranes were tested in vitro, and the compatibility properties were detected by MTT method. The activity of ALP was assayed by PNPP method. The data was analyzed by SPSS 16.0 software package. Results: Membranes with different concentration of CS/β-GP could release protein slowly more than 12 days, and the total quantity of the released protein increased with the concentration of the β-GP. The changes of mechanical properties of the membranes were not significant (P>0.05). The OD value of group A, B and C had statistically significant difference in the MTT test. The values of group A and B were higher than that of group C, while the value of group A was higher than that of group B (P<0.05). The activities of ALP were different in the three groups. The activities of group A and B were higher than that of the blank control group (P<0.05).The difference in expression of ALP between group A and B was also statistically significant. The expression in group A was higher than that in group B (P<0.05). Conclusions: The new type CS/β-GP membrane shows property of guided bone regeneration in vitro, which have the potentials for clinical use. Supported by Science and Technology Development Planning Project of Yantai City(2013WS248) and Science and Technology Award Program for Young and Middle-aged Scientists of Shandong Province(BS2013YY056).
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To investigate lowered serum estrogen on the expression of ERα, ERβ, OPG and RANKL of rat condyle and explore the possible mechanisms of idiopathic condylar resorption(ICR). Methods: Thirty female SD rats were randomly divided into control group, oral contraceptives group and tripterygium glycosides tablets group. The model of lowered serum estrogen level and model of premature ovarian were established by gastric gavage with oral contraceptives pills and tripterygium wilfordii glycosides tablets, respectively. The serum levels of estradiol were detected respectively before experiment and 12 weeks after experiment. After experiment, the expression of ERα, ERβ, OPG and RANKL of condyle were detected by immunohistochemistry technology. SPSS16.0 software package was used for statistical analysis. Results: The serum levels of estradiol were significantly decreased in both experimental groups. ERα, ERβ, OPG and RANKL were expressed in the condylar cartilage in all groups, mainly distributed in the hypertrophic cartilage layer. In oral contraceptives group and tripterygium glycosides tablets group, rat condylar ERα, ERβ and OPG expression were decreased and RANKL expression was increased. Conclusions: The lowered serum estrogen concentrations decrease the expression of ERα, ERβ and OPG, raise the expression of RANKL of condylar cartilage and reduce the proportion of OPG/RANKL. In the lowered serum levels of estrogen, estrogen may mediate condylar cartilage degeneration and the subchondral bone pathological reconstruction through ER pathway by regulating the ratio of OPG/RANKL. Supported by Science and Technology Plan Project of Hefei City [(2013)183-37].
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To study the value of ultrasonic knife in submandibular gland surgery. Methods: Randomized controlled clinical trial was adopted. Forty cases collected from October 2013 to July 2014 who underwent submandibular gland surgery were divided into experimental group and control group. All cases were operated by the same surgical team. Ultrasonic knife was used in the experimental group, while electric knife was used in the control group. The operation time, intraoperative blood loss, postoperative complications and 24 h and 48 h postoperative drainage volume were recorded and compared between the 2 groups. SPSS 10.0 software package was used to analyze the data. Results: There was no significant difference in age, gender, mass size and disease type between the 2 groups (P>0.05). With the same operative procedure, there was no significant difference in postoperative complications (P>0.05). Compared with the control group, the operation time, intraoperative blood loss and 24 h and 48 h postoperative drainage volume were significantly decreased in the experimental group (P<0.05). Conclusions: Using ultrasonic knife in submandibular gland surgery is safe and reliable, with distinct advantages over electric knife. The tissue damage is less, and postoperative complications are minimal. It is worth of wide application in oral and maxillofacial surgery.
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To identify the relationship between hyperglycemia levels and periodontitis by investigating the levels of hyperglycemia and periodontal conditions of officeholders in Nantong city. Methods: From January 2013 to January 2014, 545 officeholders were randomly selected in Nantong City who underwent physical examination and divided into 2 groups: young of middle-aged adults(≤60 years) and old adults (>60 years). The data was analyzed with Chi-square test and multi-factors logistic regression by using SPSS 16.0 software package. Results: There was a significant correlation between blood glucose levels and periodontitis. Multi-factor Logistic regression analysis showed that in the whole population, smoking, age, blood glucose levels were significantly correlated with the degree of periodontitis (P<0.05). Smoking and low level of education were associated with periodontitis in young and middle-aged adults. In old people, blood glucose level more than 7.0 mmol/L and smoking were the risk factors for periodontitis. Conclusions: Hyperglycemia level is risk factor for periodontitis, which has an effect mainly on old adults. Supported by Key Project of Science and Technology Bureau of Jiangsu Province (BL2013002) and Social Science and Technology Innovation and Demonstration Projects of Nantong City(HS2013046).
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To evaluate the effectiveness of tooth extraction and non-extraction orthodontic treatment on frontal smiling esthetics. Methods: A literature research was performed using Wanfang database, Chinese Biological Literature database, China National Knowledge Infrastructure, Chinese Scientific Journals Database of VIP, Medline and the Cochrane Library, dating from the establishment of the databases to 31st, August, 2014. Weighted mean difference (WMD) was calculated and meta analysis was performed by Review Manager 5.2. Results: A total of 8 controlled studies were included. The results of meta analysis showed no significant difference between extraction and non-extraction treatment on subjective evaluation of smile esthetics [5.74-7.05 for extraction; 5.53-7.02 for non-extraction; WMD=0.09, 95%CI (-0.28, 0.46), P=0.64], buccal corridor [0.12-0.19 for extraction; 0.11-0.18 for non-extraction; WMD=0.01, 95%CI (-0.00,0.02), P=0.09], maxillary visual arch width [26.3-52.17 mm for extraction; 25.43-52.37mm for non-extraction; WMD=-0.13, 95%CI (-1.01, 0.75), P=0.77] and smile height [5.7-10.39mm for extraction; 5.4-9.97mm for non-extraction; WMD=0.38, 95%CI (-0.27, 1.03), P=0.25]. Conclusions: Based on the results of this meta analysis, it can't be concluded that extraction treatment could affect the frontal smiling esthetics based on the present clinic evidences. Given the small sample size and the potential heterogeneity, more well-designed prospective studies should be performed in future. Supported by National Creative Planning Project for College Students(201310422049) and Science and Technology Planning Project of Shandong Province (2010G0020232).
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Pulp stones were denaturation of pulp tissue, which were usually found in the pulp chamber. Generally, they were associated with caries and pulposis, and the occurrence of pulp stone increased with age. Pulp stones were frequently found by radiographic examination, and appeared as radiopaque lesions which were round or ovoid in shape. We reported an unusual case of multiple pulp stones with normal clinical crowns in a young female patient and analyzed the possible etiology. Supported by Scientific Research Fund of Hebei United University (Z201333).
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Puppose: To construct quorum sensing luxS knockout mutants of Enterococcus faecalis through homologous recombination. Methods: The upstream and downstream flank DNA fragments of E. faecalis luxS gene (up, dn) and erythromycin resistance gene (erm) were amplified by PCR. In order to construct recombination plasmid Puemrd, these DNA fragments were inserted into the plasmid pUC18 by corresponding double digests. After allelic exchange, the luxS knockout mutants strains were selected on 30 μg/mL erythromycin plates. Results: With endonuclease reaction and DNA sequencing, it was proved that the objective plasmid, Puemrd, was constructed correctly. The luxS knockout mutants strains were confirmed by PCR. Conclusions: Enterococcus faecalis luxS gene has been successfully disrupted with homologous recombination. This mutant strain sets a good foundation for further functional study.
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To develope the influence of different ultrasonic irrigations after root canal preparation with nickel titanium ProTaper on micro-hardness of root canal dentin. Methods: Sixty of maxillary anterior teeth with single-canal were collected and randomly divided into 6 groups. Group A was control group, group B was prepared to F3 with nickel titanium ProTaper by machine, group C was ultrasonic irrigated with 3% hydrogen peroxide solution for 1 minute after preparation, group D was ultrasonic irrigated with koutai mouthwash for 1 minute after preparation, group E was ultrasonic irrigated with 17% EDTA solution for 1 minute after preparation, group F was ultrasonic irrigated with distilled water for 1 minute after preparation. The roots were then sectioned horizontally into 3 parts, split longitudinally into halves and examined under a micro Vickers hardness test machine. The data was analyzed by one-way ANOVA and t test with SPSS 17.0 software package. Results: The micro-hardness of group A was (52.66±1.64) HV,(52.08±1.53) HV and (51.47±2.53) HV. There was no significant difference in all parts of the root canal in group A (P>0.05). The micro-hardness of the apical third of root canal was lower than that of the cervical and middle of root canal in the other groups (P<0.05). In the cervical and middle third of the root canals, the micro-hardness of group E was (44.65±1.33) HV and(42.55±1.12) HV, and there were statistical significances between group E and the other groups (P<0.05). In the apical third of root canal,the micro-hardness of group E was (37.82±1.60) HV, and group C was (44.14±1.73) HV, both of the comparative differences with other groups were statistically significant (P<0.05). There was no significant difference among group B, group D and group F (P>0.05). Conclusions: Root canal preparation to F3 with nickel titanium ProTaper by machine can make the micro-hardness of the apical third of root canal decrease. Ultrasonic irrigation with 17% EDTA solution for 1 minute can make the micro-hardness of the root canal decrease ultrasonic irrigation with. Ultrasonic irrigation with 3% hydrogen peroxide can make the micro-hardness of the apical third of root canal decrease. Ultrasonic irrigation with Koutai mouthwash and distilled water for 1 minute have no influence on the micro-hardness of root canal.
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To investigate the effect of three different zirconia angular abutments on the stress distribution in bone and abutment using three-dimensional finite element analysis, and provide instruction for clinical application. Methods: Finite element analysis (FEA) was applied to analyze the stress distribution of three different zirconia/titanium angular abutments and bone around implant. Results: The maximum Von Minses stress that existed in abutment, bolt and bone of the angular abutment model was significantly higher than that existed in the straight abutment model. The maximum Von Minses stress that existed in abutment, bolt and bone of the 20 ° angular abutment model was significantly higher than that existed in 15 ° angular abutment model. There was no significant difference between zirconia abutment model and titanium abutment model. Conclusions: The abutment angulation has a significant influence on the stress distribution in the abutment, bolt and bone, and exacerbates as the angulation increases, which suggest that we should take more attention to the implant orientation and use straight abutment or little angular abutment. The zirconia abutment can be used safely, and there is no noticeable difference between zirconia abutment and titanium abutment on stress distribution.
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To investigate the influence of Pg-LPS stimulated monocyte (RAW264.7) culture supernatant on the OPG/RANKL expression of osteoblastic cells (MC3T3-E1). Methods: The culture supernatant of monocytes stimulated with Pg-LPS was applied to osteoblasts MC3T3-E1 with different diluted concentrations (10%, 20%, 30%, 40% and 50%) simultaneously for 24h, then RT-PCR was used to detect the expression changes of OPG/RANKL mRNA. Western blot was used to detect the expression changes of OPG/RANKL protein. The data was analyzed by ANOVA using SPSS 17.0 software package. Results: After stimulation of different concentrations of inflammatory supernatant, the expressions of OPGmRNA and protein significantly decreased (P<0.05), whereas the expressions of RANKLmRNA and protein significantly increased(P<0.05), both of them were in a concentration-dependent manner. Conclusions: These results indicate that Pg-LPS stimulated RAW264.7 culture supernatant can inhibit the osteogenesis and differentiation of the osteoblasts through inhibiting the expression of OPGmRNA and protein of osteoblastic cells, while increasing the expression of RANKLmRNA and protein in a concentration-dependent manner. Supported by Natural Science Foundation of Zhejiang Province(Y207360) and Science and Technology Research Project of Wenzhou City (Y20130259).
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To compare the effect of recombinant full-length human amelogenin (rhAm) and enamel matrix proteins (EMPs) on differentiation of human bone marrow stromal cells (hBMSCs) into osteoblasts. Meanwhile, to investigate the possible mechanism of rhAm promoting osteogenic differentiation of hBMSCs. Methods: The hBMSCs were cultured in vitro. The cells were treated with 10 μg/mL rhAm and 200 μg/mL EMPs. The gene and protein expression of Runx2, ALP, Col-I were observed by using RT-PCR and Western blot at different time points. The influence of rhAm and EMPs on mineralization and osteogenesis of hBMSCs were observed by using alkaline phosphatase and alizarin red staining methods. The data was analyzed with SPSS 13.0 software package. Results: Both rhAm and EMPs significantly promoted gene and protein expression of Runx2, ALP and Col-I in hBMSCs. Meanwhile, rhAm and EMPs also facilitated osteogenesis and mineralization of hBMSCs. The effects of two proteins on hBMSCs had no significant difference. Conclusions: Both 10 μg/mL rhAm and 200 μg/mL EMPs can significantly promote differentiation of hBMSCs into osteoblasts. The rhAm may be used in inducing periodontal tissue regeneration in the future. Supported by National Natural Science Foundation of China(81070838, 81271156) and Biomedical Engineering Cross Research Foundation of Shanghai Jiao Tong University(YG2011MS31).
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To establish a standard system of composite resins restoration in dental undergraduate education. Methods: The modern phantom system was improved. A step-by-step operating manual with large amount of photographs and contents was set up, and multimedia tools were used simultaneously. A set of standardized operative procedures and detailed score evaluation system were established. Questionnaires were used to assess students' satisfaction in the reform. Then the survey results were statistically analyzed by Fisher exact test with SPSS 18.0 software package. Results: The students' satisfaction about the education of composite resin restoration were improved (P<0.001). This reform helped to deepen students' comprehension of the theories and standardize their manipulation. Conclusions: The establishment of a standardized teaching procedure is beneficial to improve the quality of dental undergraduate education in composite resin restoration.
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To evaluate the methodology and reporting quality of Chinese systematic reviews and meta analysis in oral medicine. Methods: Chinese literatures of systematic reviews and meta analysis in oral medicine were searched in the CBM, VIP, WANFANG Database and CNKI from the establishment date to August 30, 2014. Two researchers screened and evaluated the data independently, and disagreements were resolved by discussion. Methodology and reporting quality of included literatures were evaluated by AMSTAR and PRISMA scale. Results: Of the 204 literatures included in the analysis, the highest and lowest scores of methodology quality were 9 and 0, respectively. The average score was 4.95±2.45. The main problems were insufficient in literature searching, absence of a list of included and excluded studies, lack of assessment for publication bias, etc. The highest and lowest scores of reporting quality were 21 and 4, and the average score of reporting quality was 14.07±3.62. The main problems were incomplete report in abstract, data collection and analysis methods, bias control, conclusion, etc. Conclusions: The methodological qualities of Chinese systematic reviews and meta analysis in oral medicine are generally low, and their reporting qualities are also needed to be improved.
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To explore the vertical and horizontal proportions of the face and difference between the Han and Uygur normal adults in Xinjiang area. Methods: According to certain standards, the adult Han and Uygur college students in Urumqi City were randomly selected for this study. These students were divided into male and female groups. Frontal photographs were taken for every subject in accordance with same standards. The data was imported into computer, with 20 standard anthropometric marks be determined, and 15 measurements were performed through Photoshop software. Independent sample t test was applied for measuring results by SPSS19.0 software package. Results: The face of males were shorter and wider than that of females. Compared with the females, the males had bigger alar width. However, the Han adults had smaller palpebral fissure and mouth width, bigger alar and intercanthal compared with the Uygur adults. Conclusions: This study suggests that there are significant differences between 2 nationalities and sexes in facial proportions in Xinjiang area.
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To evaluate the effect of dental health instruction before treatment on dental anxiety of patients with acute pulpitis. Methods: One hundred and fifty-four patients with acute pulpitis treated in our department from July 2011 to June 2013, and aged from 19 years to 64 years, were selected. They were randomly divided into experimental group and control group. Seventy-eight patients of the experimental group accepted dental health instruction before treatment, while 76 cases in the control group received regular treatment. Two questionaires of dental anxiety were proceeded to both groups respectively before treatment. The data was analyzed for Student's t test and Chi-square test using SPSS12.0 software package. Results: Dental anxiety (DA) points of the experimental group after dental health instruction were significantly lower than that before treatment (t=4.1346, P<0.01). DA points of the experimental group after dental health instruction were significantly lower than that of the control group (t=6.2784, P<0.01). The acceptability of the experimental group towards the first treatment was significantly higher than of the control group. The completion rate of the following treatment of the experimental group was also significantly higher than that of the control group. Conclusions: Dental health instruction to patients with acute pulpitis before treatment are helpful to reduce the pressure and relieve the anxiety during the treatment, so that the patients will complete the first and the following treatment successfully.
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To analyze the changes in epithelial cells, and observe the expression of Bcl-2, Bax and apoptosis in oral lichen planus. Methods: Thirty five OLP patients and 10 healthy volunteers were enrolled in this study. Immunohistochemical S-ABC was used to detect Bcl-2 and Bax expression in OLP group and control group. TUNEL and flow cytometry were used to study the apoptosis of the epithelial cells. Transmission electron microscope (TEM) was used to observe the changes of ultra-microstructure of oral membrane of OLP. The data was analyzed with SPSS 17.0 software package. Results: The expression of Bcl-2 in OLP group was not significantly different from control group (P>0.05), but the expression was remarkably elevated in lymphocyte infiltration zone. Bax positive expression rate and intensity in OLP group significantly increased compared with the control group (P<0.01). The apoptosis index was remarkably increased in the epithelial cells in OLP group compared with the control group (P<0.01), which was consistent with the result of flow cytometry(the apoptotic percentage of the epithelial cells in OLP group was significantly higher than the control group). TEM showed that the apoptotic cells of mucous membrane of OLP increased, with chromatin margination, and apoptotic body. Conclusions: The ultra-microstructure change in the lesional tissue, increased apoptotic cells, elevated Bcl-2 in lymphocyte infiltration zone and elevated Bax in epithelial basement keratin suggest that apoptosis may be related to the carcinogenesis and development of OLP. Supported by Basic and Advanced Science and Technology Research Project from Henan Province (142300410341).
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To observe the effects of calcium hydroxide Ca(OH)2 and Hoshino's paste(a mixture of metronidazole, ciprofloxacin, and minocycline) on the proliferation of porcine dental papilla cells (pDPCs), and to provide some theoretical basis for the choice of root canal disinfection drugs for pulp revascularization treatment. Methods: The 4th generation of pDPCs were treated with several concentrations of Ca(OH)2 (10%,1%,0.1%) and Hoshino's paste (0.5%, 0.1%, 0.05%) medium for certain time periods. CCK-8 assay was performed to detect the effect of the 2 drugs on cell proliferation. SPSS19.0 software package was used for one-way ANOVA. Results: The effect of Ca(OH)2 on pDPCs proliferation was fluctuating, but cell proliferation of each Ca(OH)2 group had no difference after 9 days. Meanwhile, cell proliferation was inhibited by Hoshino's paste. Conclusions: Each concentration of Ca(OH)2 in the study has no effect on pDPCs proliferation, while Hoshino's paste can inhibit cell proliferation. Supported by Medical Guide Project of Science and Technology Committee of Shanghai Municipality (124119b0101).
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To observe arctigenin's inhibitory effect on oral squamous cell carcinoma, and explore the possible mechanism. Methods: The expression of VEGF in 32 cases of oral squamous cell cancer and 20 adjacent tissue specimen were detected with immunohistochemistry. Human nude mouse transplantation tumor model of oral squamous cell cancer was prepared with HSC-3 cells line. Transplanted tumor growth and VEGF expression in transplanted tumor tissues were assayed after treatment with arctigenin. One-way ANOVA was used for comparison between groups with SPSS 16.0 software package. Results: Compared with the adjacent tissue, immunohistochemical staining score of VEGF was significantly higher (P<0.01) in oral squamous cell carcinoma tissues. After treatment with arctigenin, the growth of oral squamous cell transplanted tumors in nude mouse was inhibited (P<0.05), and decreased weight in end point of observation was noted (P<0.05). There were significant differences between high dose group and low dose group (P<0.05). Compared with the nude mouse model group, the optical density of VEGF staining was significantly lower in arctigenin group (P<0.05). There were significant differences between high dose group and low dose group (P<0.05). Conclusions: Arctigenin can dose-dependently inhibit the growth of oral squamous cell carcinomas, and this effect may be related to down regulation of VEGF expression. Supported by National Natural Science Foundation of China (81102058).
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose: To analyze the effect of ffh gene silencing on the aciduricity of fluoride resistant Streptococcus mutans in vitro. Methods: By using electroporation, UA159-FR was transformed and combined with targeted site of ffh gene sequence, and the best piece of siRNA for fluoride resistant Streptococcus mutans was screened. In different values of pH of BHI, they were cultured for 24 hours with UA159-FR respectively, and then centrifugated to determine the pH and OD600. SPSS17.0 software package was used to analyze the data. Results: The aciduricity of UA159-FR had significant differences compared with ffh gene silencing for UA159-FR in δpH (P<0.05), and the former was higher than the latter. At pH=3.5-5.0, P<0.01; at pH=5.5-7.5, P<0.05. Significant differences were noted in OD600 and their growth tendency were similar. Conclusions: The aciduricity of fluoride resistant Streptococcus mutans has significant effect when the ffh gene is silenced. Supported by National Natural Science Foundation of China (3A411X463431).
    No preview · Article · Sep 2015 · Shanghai kou qiang yi xue = Shanghai journal of stomatology