Asian Journal of Microbiology, Biotechnology and Environmental Sciences

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ISSN 0972-3005

Publications in this journal

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    ABSTRACT: Aim: Sporosarcina pasteurii is one of the soil inhabitant and urease-producing microorganisms from which urease enzyme is made, causing bio-calcification. In this research, optimizing of urease enzyme produced by Sporosarcina pasteurii bacteria was targeted to connect the sand particles and stiffen them by the production of calcite precipitation. Methods: bacterium was cultivated aerobically in a culture medium containing yeast extract, lacto pepton and urea at the temperature of 25C. Different conditions of cultivation with Urea concentration calcium chloride and pH were examined to obtain optimal results. Results: The optimum condition for the stiffening of sand grains was attained in the presence of urea (20 g/L), calcium chloride (30mM) and the pH of 9. Conclusion: The results of this research indicated that the sand calcification via urease enzyme produced by Sporosarcina pasteurii could be employed for the hardening of the sand provided that the efficient factors of calcite precipitation could be optimized.
    No preview · Article · Jan 2016 · Asian Journal of Microbiology, Biotechnology and Environmental Sciences

  • No preview · Article · Jan 2016 · Asian Journal of Microbiology, Biotechnology and Environmental Sciences
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    ABSTRACT: A complex culture medium was optimized using response surface methodology in order to achieve maximum antimicrobial activity of Bacillus subtilis TS 01 against phytopathogens. Glucose concentration (X1), corn extract (in relation to nitrogen) (X2) and KH2PO4 (X3) were selected as the independent variables. Results showed that the regression models adequately explained the data variation and represented the actual relationships between the parameters and responses. The composition of two new optimized nutrient media more convenient for the production of antimicrobial activity by B. subtilis TS 01 was established, namely: 1st medium (in %): glucose 1.40, corn extract (in relation to nitrogen) 0.60, KH2PO4 0.10, MgSO4 0.01, pH 7.0 in order to reach maximum antifungal activity of the strain; 2nd medium (in %): glucose 2.50, corn extract (in relation to nitrogen) 0.56, KH2PO4 0.20, MgSO4 0.01, pH 7.0 in order to reach maximum antibacterial activity of B. subtilis TS 01. Relative to the initial medium, significantly higher both antifungal and antibacterial activity of the strain was chieved in the optimized media. In the 1st medium the activity of B. subtilis TS 01 against Botrytis cinerea was 42.00 mm (sterile zone) and in the 2nd medium.
    No preview · Article · Dec 2015 · Asian Journal of Microbiology, Biotechnology and Environmental Sciences

  • No preview · Article · Aug 2015 · Asian Journal of Microbiology, Biotechnology and Environmental Sciences

  • No preview · Article · Aug 2015 · Asian Journal of Microbiology, Biotechnology and Environmental Sciences

  • No preview · Article · May 2015 · Asian Journal of Microbiology, Biotechnology and Environmental Sciences

  • No preview · Article · May 2015 · Asian Journal of Microbiology, Biotechnology and Environmental Sciences
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    ABSTRACT: Gastric and oesophageal cancer surgery is performed to remove cancerous tissue or to by pass part of the tumour tissue to prevent blockage of the stomach or oesophagus. However, surgical operations of the gastrointestinal tract may lead to alterations in the gut microbial community. Therefore the aim of this study was to determine changes in microbial community profile in the gastrointestinal tract of pre- and postoperative gastric and oesophageal cancer patients using polymerase chain reaction - denaturing gradient gel electrophoresis (PCR-DGGE). Our results showed that changes occur in the intestinal tract after gastric and oesophageal cancer surgery but not immediately after operation.OIn oesophageal cancer patients, Collinsella sp. was detected in both pre- and post- operative subjects. Serratia marcesens, a prominent opportunistic pathogen was detected after oesophageal cancer surgery in one subject. Acholeplasma parvum which is known to survive in host environments was detected in one gastric cancer patient after six months of operation. In both gastric and oesophageal cancer patients most of the bacterial species detected belonged to Actinobacteria and Firmicutes. The data obtained in this study could provide a basis for modulation of gut microbial balance after surgery which could lead to faster recovery, and prevention of post operative bacterial infections.
    No preview · Article · Jan 2015 · Asian Journal of Microbiology, Biotechnology and Environmental Sciences
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    ABSTRACT: Amidases (EC 3.5.1.4) hydrolyzes amides with the production of the corresponding carboxylic acids and ammonia and are the most widely used amide-hydrolyzing enzymes in industry. It holds a great potential for biotechnological applications in organic acids synthesis, bioremediation, waste water treatment, synthesis of pharmaceutical chemical like anti-cancerous and anti-HIV Hydroxamic acids etc. Here, we report the purification of amidase from Bacillus sp. APB-6 and after purification the enzyme was characterized for optimum activity. The enzyme was purified by about 21.7 fold with specific activity 19.5 U/mg protein. A purified amidase enzyme of 44kDa was obtained after initially precipitated with 60% Acetone. The acetone precipitated enzyme was then loaded on to the anion exchange column (DEAE-Cellulose) and then the protein was analysed by SDS-PAGE. The optimum temperature and pH of the enzyme was 55pC and 7.5 respectively. The Km and Vmax values for the purified amidase of APB-06 were 200mM and the Vmax was 100 pmoles/mg/min The enzyme was highly specific to substrate acetamide and less activity was detected in the presence of other amides, such as butyramide, cyanamide, nicotinamide and benzamide.
    No preview · Article · Jan 2015 · Asian Journal of Microbiology, Biotechnology and Environmental Sciences
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    ABSTRACT: Experiments were carried out to evaluate the equilibrium and thermodynamic behaviour of the batch adsorption system involving the use of modified activated sludge for the treatment of refinery waste water. The equilibrium data were determined for the sorption system and fitted with three equilibrium isotherm models, namely Langmuir, Freundlich and Dubinin-Radushkevich models. Langmuir isotherm fitted well with the equilibrium data with high values of correlation coefficient and the maximum COD uptake capacity was determined from the linear plot as 147.06 mg/g at an operating temperature of 30°C. The thermodynamic studies were conducted to evaluate the Gibbs free energy and the negative values observed in this study confirmed the spontaneous nature of sorption. The enthalpy change was determined as-18.012 kj/mol indicating the exothermic nature of the process.
    No preview · Article · Jan 2015 · Asian Journal of Microbiology, Biotechnology and Environmental Sciences
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    ABSTRACT: The purpose of this study was to examine the antibacterial potency of seaweed and to analyzes the ice-ice disease control through polyculture cultivation techniques. This research was conducted in two phases, namely laboratory and field study. The method used in the laboratory study is designed with a completely randomized design. The antibacterial activity assays performed with the agar diffusion method. Data of the inhibition zone diameter were analyzed by one-way analysis of variance followed by honestly significant difference test at a significance level of 5 %. Data of the field study collected base on appearance of the thallus and the percentage of infected clumps and analyzed descriptively. Data on wet weight gain per week were analyzed by t test. The results showed that the antibacterial activity of seaweed Achantophora spicifera, Caulerpa racemosa, Sargasum duplicatum and Lorencia obtusa provide a significantly different effect on (a using) isolates of Pseudomonas sp A of ice-ice disease seaweed. Achantophora spicifera showed stronger potency in inhibiting bacterial test so it was decided to be used as companion plants in biological control. The field study suggests that ice-ice disease in Kappaphycus alvarezii can be controlled through polyculture techniques. The result showed that in the treatment group 0.062 % infected by ice-ice disease, while in control group more than 50%. Therefore polyculture techniques can be used to control the ice-ice disease using seaweed Achantophora spicifera.
    No preview · Article · Jan 2015 · Asian Journal of Microbiology, Biotechnology and Environmental Sciences

  • No preview · Article · Jan 2015 · Asian Journal of Microbiology, Biotechnology and Environmental Sciences