JPC - Journal of Planar Chromatography - Modern TLC (JPC-J PLANAR CHROMAT)

Publisher: Akadémiai Kiadó

Journal description

Journal of Planar Chromatography- Modern TLC is an international journal devoted exclusively to the publication of research papers on analytical and preparative planar chromatography. The journal covers all fields of planar chromatogeaphy, on all kinds of stationary phases (paper, layer, gel) and with various modes of migration of the mobile phase (capillary action of forced flow).

Current impact factor: 0.67

Impact Factor Rankings

2016 Impact Factor Available summer 2017
2013 Impact Factor 0.67
2012 Impact Factor 0.955
2011 Impact Factor 0.767
2010 Impact Factor 1.247
2009 Impact Factor 0.662
2008 Impact Factor 0.982
2006 Impact Factor 1.153
2005 Impact Factor 0.667
2004 Impact Factor 0.824
2003 Impact Factor 0.879
2002 Impact Factor 1.047
2001 Impact Factor 0.555
2000 Impact Factor 1.118
1999 Impact Factor 1.03
1998 Impact Factor 1.321
1997 Impact Factor 0.643
1996 Impact Factor 1.096
1995 Impact Factor 0.759
1994 Impact Factor 1.394

Impact factor over time

Impact factor

Additional details

5-year impact 0.79
Cited half-life 6.90
Immediacy index 0.16
Eigenfactor 0.00
Article influence 0.19
Other titles JPC
ISSN 0933-4173
OCLC 160109042
Material type Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details

Akadémiai Kiadó

  • Pre-print
    • Author can archive a pre-print version
  • Post-print
    • Author can archive a post-print version
  • Conditions
    • Authors own final version only can be archived
    • Publisher's version/PDF cannot be used
    • On author's personal website or institutional repository or any repository mandated by Author's funding body
    • Published source must be acknowledged
    • Must state that the file is not the final published version of the paper
    • Must link to publisher version([DOI of the Article without brackets])
    • Articles in some journals can be made Open Access on payment of additional charge
  • Classification

Publications in this journal

  • No preview · Article · Dec 2015 · JPC - Journal of Planar Chromatography - Modern TLC
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    ABSTRACT: A high-performance thin-layer chromatographic (HPTLC) method for the analysis of the protostemonine from Stemona sessilifolia (Miq.) Miq. has been developed and validated. According to the HPTLC fingerprint, 12 common peaks were selected to evaluate the similarities among 10 batches of samples with RF values ranging from 0.02 to 0.95. Ten different batches of samples were stable and reliable in quality, all the similarities of which are more than 0.98. The results indicated that this method was sensitive and precise for the quality evaluation of S. sessilifolia samples.
    No preview · Article · Dec 2015 · JPC - Journal of Planar Chromatography - Modern TLC
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    ABSTRACT: The aim of this study was to compare the antioxidant activity of crude extracts of differing polarities of the fruits of Chaenomeles speciosa (Sweet) Nakai. The antioxidant compositions of the extracts were analyzed qualitatively and quantitatively, respectively. The 75% ethanol extract of the dried fruits was fractionated by sequential extraction using petroleum ether, ethyl acetate, and n-butyl alcohol. The antioxidant effectiveness of the components of differing polarities was examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging method and compared with two reference substances: ascorbic acid and butylated hydroxytoluene (BHT). The total phenolic content of the extracts was analyzed using the Folin-Ciocalteu method and expressed as gallic acid equivalents. The active compounds were analyzed by thin-layer chromatography (TLC)-bioautography and ultra-performance liquid chromatography (UPLC). The order of antioxidant capacities of various solvent extracts from the fruit of C. speciosa was found to be ethyl acetate ≥ n-butyl alcohol > petroleum ether. The ethyl acetate extract was more active than the reference substances ascorbic acid and BHT. The radical-scavenging capacity of the extracts decreased as the total phenolic content decreased. TLC-bioautography revealed that the ethyl acetate extract contained many antioxidant spots that can remove DPPH radical, and protocatechuic acid and chlorogenic acid were the major antioxidant components. UPLC analysis confirmed that protocatechuic acid and chlorogenic acid were mainly distributed in the ethyl acetate fraction. The study demonstrated that the ethyl acetate extract had excellent antioxidant capacity. The total phenolic, protocatechuic acid, and chlorogenic acid contents of this extract were higher than those of the other two solvent extracts. These results showed that the ethyl acetate extract from the fruit of C. speciosa could be considered as a potential source of natural antioxidant agent.
    No preview · Article · Dec 2015 · JPC - Journal of Planar Chromatography - Modern TLC
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    ABSTRACT: This work presents a comparative study on the development and validation of two analytical techniques applied for the simultaneous determination of hydrocortisone acetate (HCA), fusidic acid (FSA), methyl paraben (MPB), and propyl paraben (PPB) formulated as a topical cream. The first technique was thin-layer chromatography (TLC)-densitometric method, which was developed by separating the four components on silica gel 60 F254 using methylene chloride-methanol-benzene in the ratio of 10:2:5, v/v, as the developing system, followed by densitometric measurement of the bands at 240 nm. The second technique was the chemometric method using two models: principle component regression model (PCR) and partial least squares (PLS). The suggested techniques were validated in compliance with the International Conference on Harmonization (ICH) guidelines and were successfully applied for the determination of the quaternary mixtures as prepared synthetically in laboratory and in the commercial topical pharmaceutical formulation.
    No preview · Article · Dec 2015 · JPC - Journal of Planar Chromatography - Modern TLC
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    ABSTRACT: Combining the compared study, error analysis, and comprehensive discussion, we can get the following conclusions. There is a closed liner relationship between the amount of TLC spots and pixel. TLCPQ shows good reproducibility and accuracy, so it can be put to use in quantitative research. The background has interference for determination. Nevertheless, it is not applicable when the independent variable "x" is too small. The establishment of a novel TLCPQ method provides a convenient and practical way to measure a single component content, to measure the content in multicomponent products simultaneously, and to study drug synthesis and natural product extract technology.
    No preview · Article · Dec 2015 · JPC - Journal of Planar Chromatography - Modern TLC
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    ABSTRACT: A simple, rapid, and sensitive high-performance thin-layer chromatographic (HPTLC) method was developed for the estimation of protodioscin from Trigonella foenum-graecum. Chromatographic separation was achieved on aluminum plates precoated with silica gel G60 F254 as the stationary phase and chloroform-methanol-glacial acetic acid (6.5:3.0:0.5 v/v) as the mobile phase. Densitometric evaluation was carried out at 515 nm after derivatizing the spots with p-dimethyl aminobenzaldehyde. The RF value of the protodioscin was found to be 0.31 ± 0.02. The response in terms of peak area was found to be linear over the concentration range from 400 to 1400 ng spot-1 with a regression coefficient of 0.9981 and mean recovery of 100.90 ± 3.00%. The developed method was validated as per the International Conference on Harmonization (ICH) guidelines. The limit of detection and limit of quantitation were found to be 100 ng spot-1 and 400 ng spot-1, respectively. Robustness of the developed method was assessed using experimental design, and results were analyzed statistically and graphically. The method was found to be accurate, precise, and robust to carry out the estimation of protodioscin from the plant matrix. The developed method was successfully applied for the estimation of protodioscin from marketed herbal formulations and the seeds, leaves, and roots of T. foenum-graecum.
    No preview · Article · Dec 2015 · JPC - Journal of Planar Chromatography - Modern TLC
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    ABSTRACT: Imidacloprid is a member of chloronicotinyl neonicotinoid compounds. It is the most important systemic insecticide and has a wide diversity of uses: in agriculture, on turfs, on pets, and for household pests. During the last decade, Forensic Science Laboratories of Maharashtra State, India, detected a large number of human poisoning cases with imidacloprid. Since a large number of biological samples were received for toxicological analysis, thin-layer chromatography (TLC) was the method of choice. This study reports that cobalt thiocyanate was found to be a selective and sensitive spray reagent for imidacloprid in routine analysis by TLC. Imidacloprid reacts with cobalt thiocyanate which gives intense blue-colored compound. The cobalt thiocyanate reagent does not react with the organochlorine insecticides, organophosphorus insecticides, and pyrethroids. Visceral constituents (amino acids, peptides, proteins, etc.) do not interfere.
    No preview · Article · Oct 2015 · JPC - Journal of Planar Chromatography - Modern TLC
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    ABSTRACT: Despite the fact that many researchers have developed different methods for the determination and detection of bile acid sequestrant, the analytical instruments are still quite costly, and expertise is usually required. In order to avoid the above-mentioned problems, simple, rapid, effective, and inexpensive methods to evaluate bile acid-binding are essential. The purpose of this study was to develop a simple and rapid thin-layer chromatography (TLC)-image analysis method to quantitatively determine the bile acid-binding activity of ten edible ethanolic plant extracts. The method was carried out by using TLC silica gel 60 F254 plate with the mobile phase composed of chloroform, methanol, and acetic acid (7:2:1 v/v). The chromatograms were analyzed with image analysis software (ImageJ). The method provided good linearity, accuracy, reproducibility, and selectivity for the determination of bile acid-binding activity. Litsea petiolata extract was found to show the most potent bile acid-binding activity compared to other extracts. Moreover, some extracts exhibited selective binding ability to bile acid; for example, extracts of Careya sphaerica and Polygonum odoratum could selectively bind rather to taurocholic acid than glycodeoxycholic acid. This study shows a simple method for the evaluation of potential edible plants to be used as cholesterol-reducing agents due to their bile acid-binding activity.
    No preview · Article · Oct 2015 · JPC - Journal of Planar Chromatography - Modern TLC
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    ABSTRACT: Dyslipidemia, which may be manifested by elevation of the total cholesterol concentration in the blood, is one of the most common medical conditions that affect individuals. This work presents a simple, fast, and precise high-performance thin-layer chromatography (HPTLC) technique for the multicomponent analysis of rosuvastatin (Ros) and fenofibrate (Fen), Ros and ezetimibe (Eze), and Ros and aspirin (Asp) in three different pharmaceutical combos prescribed for the treatment of dyslipidemia. Techniques were performed on silica gel 60 F254 TLC precoated aluminum plates with a simple mobile phase combination of toluene-acetone-acetic acid in the ratio of 6:2:0.2 v/v and produced well-resolved compact spot for all titled drugs. The linear regression statistics has shown a good linear relationship over a concentration range of 500-4000 ng spot1 for all cited drugs with a good correlation coefficient of 0.9999, and densitometric detection was carried out in the absorbance mode at 254 nm. The sensitivity of the developed technique was assessed in terms of the limit of quantification and the limit of detection. The stability study disclosed that all four drugs were stable at room temperature up to 12 h. The cited drugs were validated as per International Conference on Harmonization (ICH) guidelines. The technique was found to be specific and selective since no extra peak was recorded alongside with the main peak. The robustness study, recovery study, and the percentage of the assay of the formulations were found within the limit as per ICH guidelines. Marketable tablets were also analyzed for five different brands by the developed methodology with no interference detected from the excipients in the formulations. The cost-effectiveness, sensitivity, and selectivity of the proposed technique recommend its applicability for the routine quality-control analysis of any of the named drugs in their pharmaceutical preparations.
    No preview · Article · Oct 2015 · JPC - Journal of Planar Chromatography - Modern TLC
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    ABSTRACT: A new spectrodensitometric method was developed for the simultaneous determination of a quaternary mixture of antihyperlipidemics containing niacin, atorvastatin, and bezafibrate with either ezetimibe or simvastatin. Densitometric analysis was carried out using high-performance thin-layer chromatographic (HPTLC) silica gel 60G F254 plates as the stationary phase. The plates were developed with benzene-ACN-n-butanol (7:2:1, v/v) + 1.50%, v/v, glacial HOAC in absorbance mode at 242 nm. The retention factors of niacin, atorvastatin, bezafibrate, ezetimibe, and simvastatin were 0.17, 0.38, 0.51, 0.65, and 0.66, respectively. The method was validated according to the United States Pharmacopeia and National Formulary (USP 31-NF 26) and the International Conference on Harmonization (ICH) guidelines. Linearity ranges of all studied drugs were found to be in the range of 15-650 ng band1 with correlation coefficient values of 0.9975 or more. Limits of detection and quantitation were 5-50 and 15-150 ng band1, respectively. Upon applying polynomial regression to the same concentration ranges of standard solutions of all investigated drugs as well as spiked bezafibrate samples to rabbit plasma, correlation coefficient values had greatly improved. The proposed method was successfully applied for the simultaneous determination of the studied antihyperlipidemic drugs in plasma and in their pharmaceutical formulations. The developed method was utilized to study the pharmacokinetic behavior of bezafibrate and its drug-drug interaction with atorvastatin in rabbit males. This study has proven the increased myotoxicity risk upon coadministration of atorvastatin with bezafibrate.
    No preview · Article · Oct 2015 · JPC - Journal of Planar Chromatography - Modern TLC
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    ABSTRACT: An efficient and fast microwave-Assisted extraction (MAE) technique was developed for extracting trigonelline as an indicative biomarker for the quality control of Trigonella foenum-graecum seeds. The MAE procedure was optimized and compared with other conventional extraction techniques. The optimal conditions of MAE were 50% methanol as solvent, solid-liquid ratio 1:20 g mL1, irradiation power 40%, and two extraction cycles, 3 min each. The proposed extraction technique produced a maximum yield of 7.89 mg g1 trigonelline in 6 min which was 1.28 and 2.20 times more efficient than 3 h of heat reflux and 15 h of maceration extraction, respectively. Furthermore, rapid high-performance thin-layer chromatographic and high-performance liquid chromatographic methods for the quantification of trigonelline were established and validated. The methods were found to be simple, sensitive, precise, accurate, and specific for the estimation of trigonelline in T. foenum-graecum seeds extract and overcame disadvantages of previously published methods.
    No preview · Article · Oct 2015 · JPC - Journal of Planar Chromatography - Modern TLC
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    ABSTRACT: Simple, accurate, precise, sensitive, and validated high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC)-densitometric methods were developed for the simultaneous determination of carbinoxamine (CAR), pholcodine (PHL), and ephedrine (EPH) in antitussive syrup. In method A, reversed-phase (RP)-HPLC analysis was performed on an Inertsil CN-3 column (250 mm × 4.6 mm, 5 μm), using a mobile phase consisting of acetonitrile-distilled water (pH 3.5) using orthophosphoric acid in the ratio 70:30 (v/v) and flow rate of 1.5 mL min-1. Quantitation was achieved with ultraviolet (UV) detection at 220 nm. In method B, TLC analysis was carried out on an aluminum-backed sheet of silica gel 60 F254 layer using chloroform- propanol-ammonia (6:4:0.1, v/v) as the mobile. Quantification was carried out with UV detection at 245 nm. The validation of the proposed methods was applied according to the International Conference on Harmonization (ICH) guidelines. The suggested methods were successfully applied for the determination of the cited drugs in bulk powder and commercial dosage form.
    No preview · Article · Aug 2015 · JPC - Journal of Planar Chromatography - Modern TLC