Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia

Publisher: Societătea de Bacteriologie, Virusologie, Parazitologie, Epidemiologie; Uniunea Societăților de Științe Medicale

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Other titles Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia, Bacteriologia, virusologia, parazitologia, epidemiologia
ISSN 0376-4494
OCLC 1168398
Material type Periodical
Document type Journal / Magazine / Newspaper

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: The development of the methyl red reaction (MR) in Klebsielleae, analyzed during a 4-day thermostatting, showed important pH-variations. Two steps are distinguished during the reaction dynamics: the former of acidification, common to all Enterobacteriaceae and the latter of alkaline reversion, specific to Enterobacter, Klebsiella, Serratia and other MR germs. The processes determining the alkaline reversion are conditioned by the presence of large amounts of air/oxygen (aerobic processes). In this situation, the lack of oxygen and maintenance of a relative degree of anaerobiosis block realkalinization (media covered with paraffin oil) whereas the large aeration stimulates it (optimum ratio between the large contact surface and a small volume of medium). Alkaline reversion cannot be explained by the more or less intense process of glucose fermentation but by degradations of nitrogenized substances with formation of amines, ammonia, and other basic compounds. That is why the analysis of the reaction mechanism of methyl red has to consider the characteristics of the catabolism of the nitrogenized substances and the intense realkalinization capacity of the culture medium proper to Klebsielleae but poorly expressed and non efficient in Enterobacteriaceae.
    No preview · Article · Jan 1990 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: During the period January 1985-July 1988, 532 purulent CSF taken from patients with meningitis, aged between 3 weeks and 91 years, were studied by microscopic examination, cultivation and for H. influenzae type B (HITB) also by coagglutination (COA), counterimmunoelectrophoresis (CIE) and double immunodiffusion (DID) in agarose gel. Positive CSFs were taken from the patients aged 1 month-24 years old, of which 76% from children under 5 years old, and 42% from children under one year. 65.9% of the patients were males; the disease was more frequent in the first and last 4 months of the year, with the highest incidence in April. 12 bacterial spectra were found: N. meningitidis--62.97%, Str. pneumoniae--9.77%, H. influenzae type B--8.27%, and also Salmonella, E. coli, Staphylococcus, Klebsiella, Acinetobacter, beta-hemolytic Streptococcus, Alcaligenes, Proteus and Enterobacter in 4.70; the rest of 14.28% had indefinite etiology. H. influenzae was evidenced in CSF by microscopic examination in 3.38%, by cultivation in 3.94%, and the soluble antigen of HITB by COA in 8.27%, by CIE in 8.08% and by DID in 7.33%. The sensibility order of the tests was: COA, CIE, DID, cultivation and microscopic examination. The COA and CIE techniques are recommended for the current use in examination of the purulent CSF due to their simplicity, rapidity, sensibility, specificity and possibility of establishing the diagnosis when the bacteriologic techniques are negative.
    No preview · Article · Jan 1990 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia

  • No preview · Article · Jan 1990 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: The present paper reports data referring to the mechanism of the CNS degenerating process of prion origin, the role of the biological timing in prion diseases, the interrelations between the prion diseases and Alzheimer's disease, new data on the normal cell protein PrPc and prion protein in the PrPsc scrapie, the physico-chemical properties of the prion PrP-RN Am properties the presence of the nucleic acid in the structure of prion, interrelations between prion and AIDS/VISNA retroviruses. The paper underlines the importance, for the next decade of the molecular biology studies for clearing up: the complete structure of prion, the prion multiplication, the role of nucleic acid for the infectivity, at the molecular level, and molecular epidemiology of these cases.
    No preview · Article · Jan 1990 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia

  • No preview · Article · Jan 1990 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: The paper reports on the biochemical characteristics and the antibiotic resistant phenotypes in 18 strains of H. influenza (17 b serotype and a strain of c serotype) isolated from the CSF of the patients with purulent meningitis. The following were found: --all the 16 viable strains have homogeneous biochemical characteristics, typical for H. influenza; --the viable strains were: 7 biotype, 4 biotype II, 1 biotype III, 3 biotype IV and 1 biotype VII; --68.8% of the strains had the following antibiotic resistant phenotypes: Ap, Km, Rf, ApKm, ApRf, ApKmTc, ApKmRfT and ApCmFrKmRfTc; --the strains of the biotypes II and IV have the highest resistance to antibiotics, as concerns frequency and phenotype; --the frequency of the resistant strains is of 56.2% for ampicilline, 18.7% for tetracycline, 31.2% for rifampicine and 6.2% for cholarmphenicol.
    No preview · Article · Jan 1990 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: The efficiency was studied, in experimental and practical conditions, of media for the isolation of C. pylori, as well as criteria for diagnosis and identification. The highest sensitivity was obtained with Columbia gelose, and a lower sensitivity was achieved with Campy I. C. gelose, and with heart-brain gelose, prepared as chocolate gelose, and Columbia gelose with integral blood. Both variants of the Mueller-Hinton gelose, even in the chocolate formula, gave negative results. Of the fluid media, supplemented with blood or with serum, only broth for I. C. blood cultures, and the heart-brain broth permitted the development of small cultures, while the soya-tripticase broth and the Mueller-Hinton broth remained sterile. None of the media, either solid or fluid, as such or supplemented with factors X, V, or X + V, did allow the development of C. pylori. In natural conditions, by inoculating 92 samples of gastric mucosa from patients with gastritis, with or without ulcers, confirmed histologically on Columbia chocolate gelose as such, or in a selective variant, 50 positive results were obtained with both variants, 9 positive results on the selective variant, and one positive result on the nonselective variant. The direct microscopic examination of samples from the mucosa disclosed the presence of C. pylori in all 60 samples that had also been confirmed by culture, as well as in another 21 samples from a total of 32 samples with negative cultures (88%). The direct urease test performed directly from the sample was done in 90 cases and was positive in 64 out of 79 samples that had been confirmed bacteriologically (77.2%), but in none of the 11 negative samples. The positive prediction index is thus of 100%, and the negative index is of 37.9%. Procedures for identification consisted in a definition of morphological characteristics, and in the cultivation, and biochemical features (including catalase and oxydase determination), which can define the Campylobacter genus, and the urease test, the sensitivity to cephalotine and the resistance to nalidixic acid for the differential species diagnosis. In conclusion cultivation of C. pylori presumes the use of media with superior quality peptones, supplemented with blood lactate in the gelose chocolate formula. The direct urease test is very useful as a procedure for diagnosis, and this can be done in the departments of gastroenterology.
    No preview · Article · Oct 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia

  • No preview · Article · Oct 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: Enterotoxin-producing Klebsiella pneumoniae was implicated in the induction of intrahospital infections in new-born babies. A total of 46 children and 4 adults (hospital personnel) were involved. Most of the subjects (82.6%) had median and light forms of gastroenterocolitis, and recovered following biological re-equilibration. In 17.39% of the cases the evolution was more severe due to advanced dehydration and secondary dissemination of the infection. Two children (approximately 4%) died. Factors that favored the dissemination of the infection were hygiene deficiencies and ignorance of functioning rules of materno-infantile units, and these included: admission to the hospital of working personnel with acute phenomena of enterocolitis; administration of sweetened solutions that were prepared without control and stored at room temperature; the "critical" point represented by the special room for "the accommodation" of the newborns, a "key-point" where infection was disseminated to other wards following dispersion of "adapted babies".
    No preview · Article · Oct 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia

  • No preview · Article · Oct 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: The authors describe the evidencing of the O157H7 E. coli serotype--an enterohaemorrhagic strain of E. coli--for the first time in our country. This germ is incriminated in haemorrhagic enteritis of children and adults, accompanied or not by an uremic haemolytic syndrome. A total of 717 strains were investigated, obtained from cases of haemorrhagic enteritis (283), nonhemorrhagic enteritis (174), food poisoning (27), and a control lot of subjects without signs of enteritis (233). Identification of the strains was done with a screening method (McConkey) with D-sorbitol in place of lactose, and with anti-O157, and anti-H7 sera. The results obtained have indicated a total of 37 positive strains in haemorrhagic enteritis (69.8%), 12 strains in nonhemorrhagic enteritis (22.65%), and 4 strains in food poisoning (7.55%). No strain was isolated from the control group. The percentage of isolated O157H7 E. coli strains in our county is of 7.55% and this contributes to additional knowledge in the definition of the still unknown etiology of diarrhoeic disease in our country.
    No preview · Article · Oct 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: The authors identified a group of substances (flagellar depression factors--FDF) which suppress the mobility of enterobacteria (immobilization) but do not influence the Vibrio movements (tolerance). FDF do not affect the vital processes, multiplication and formation of colonies. Electron microscopic interpretation showed that, in the usual media, enterobacteria have characteristic peritrichous cilia, whereas in media with FDF they are denuded of their flagellation formation. As such a process of flagellation depletion seems to be not known, we suggest the term "deflagellation" to name it. In a completely different way, the vibrios keep their single cilium located apically and implicitly their moving ability. These behaviour differences are evident in semisolid agar with FDF. The deflagellated enterobacteria are, therefore, immobilized, and multiply, forming small punctual colonies, of 0.5-1 mm phi, whereas the vibrios tolerating FDF develop and at the same time move in the liquid agar, mass, forming large colonies, of 8-14 mm phi. The electron microscopic observations presented show the existence of a "deflagellation tolerance" process and also explain the specific typology of the colonies of vibrios and enterobacteria formed in liquid agar with FDF.
    No preview · Article · Jul 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: The paper reviews the connection between the human viruses and some common sanitary indicators of drinking water contamination. An analysis is made of: physico-chemical indicators (water turbidity, residual chlorine, some products of the bacterial metabolism in the intestine), bacterial indicators (total and faecal coliforms, Escherichia coli, faecal streptococci, clostridia and other intestinal anaerobic bacteria, saprophytic mycobacteria, Pseudomonas aeruginosa) and some bacteriophages. The conclusion is drawn that if such indirect indicators of water faecal pollution are generally useful in sanitary-microbiological assessment of drinking water, for finding its viral pollution, in certain epidemiological and hygiene-sanitary situations the virologic examination of the drinking water is quite compulsory.
    No preview · Article · Jul 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: The paper reports on four atypical cases of blastocystosis with prevalence of digestive and cutaneous disorders and of the allergic and general phenomena. The diagnosis could be established only after revealing Blastocystis hominis in the fecal parasitologic examination. The disappearance, in all the four cases, of varied clinical, delayed signs after treatment with metronidazol and stamycine (in two or even four repeated treatment schedules at 10 days' interval) is the proof that Bl. hominis might be the agent (unique or associated with other parasites) of some polymorphous disorders. Our findings are a call to specialists and clinicians for investigating the parasite and using an adequate treatment.
    No preview · Article · Jul 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia

  • No preview · Article · Jul 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: Carcinoembryonic anti-antigen (CEA) polyclonal antibodies were obtained in ram and rabbit using as antigen source the extracts with perchloric acid from the human colon adenocarcinomas. CEA was purified by gel filtration on Sepharose 4BCL and ion-exchange chromatography (DEAE-Sephadex A50). The total antiserum was absorbed with human serum and perchloric acid extract from the normal colon. IgG anti-CEA was purified by chromatography of the monospecific antiserum, then tested for bonding specificity, at cryostat on sections of colon adenocarcinoma, by indirect immunoperoxidase. The specific reaction was compared with that obtained by the same technique, using two monoclonal antibodies specific to the CEA molecule (MAb-26/3/13 and MAb-26/5/1 respectively). IgG anti-CEA was also used for obtaining some IgG-peroxidase conjugates, with an immunoenzymatic system, ELISA type, with two antibodies according to the model of the ELISA kits produced by the Cantacuzino Institute (ELISA-AFP). The ELISA-CEA kit was standardized using an international CEA standard. CEA was quantitatively determined with this immunoenzymatic system (ELISA-CEA) on a group of 15 healthy subjects (average: 4.8 +/- 0.12 ng/ml) and on 30 patients with colorectal tumours (average: 26.6 +/- 0.15 ng/ml). ELISA-CEA kit, sensitive and reproducible, allow the usual quantitative determination of CEA, a useful marker in diagnosing and monitoring tumour evolution.
    No preview · Article · Jul 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia

  • No preview · Article · Jul 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: The authors checked the vaccine coverage and the situations in which the compulsory vaccines were not administered up to the age of one year in the children born in February 1987 in District III of Bucharest. The results show a vaccine coverage of more than 90% in BCG vaccination (99.4%), antipoliomyelitic with one dose (94.2%) and with two doses of oral polyvaccine (92.2%) respectively, and the first DTP vaccination (95.9%). The values were under 90% in antimeasles vaccination (87.2%) and the second vaccination with 1 DTP (25.5%). The main causes leading to vaccine coverage could have been prevented. Discussion of these results shows that better vaccination values might be obtained by improving the present control on the immunoprophylaxis activity of the paediatric medical units, by intensifying both their control actions and aims.
    No preview · Article · Jul 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: The authors of the present paper consider that 82.3% of all cases of viral hepatitis in a Bucharest district were transmitted nonparenterally and only 17.7% were transmitted parenterally. They used the information on the way and mode of transmission, recorded in the epidemiologic investigations, of the cases of viral hepatitis without HBsAg in 1987 and of those with HBsAg in 1983-1987, respectively. The highest values of the risk of catching the disease were calculated for the infective social contact in family, in the viral hepatitis with HBsAg (60.9%), and for the infective social contact in collectivities (46.4%) in the viral hepatitis without HBsAg. These data have to be checked up by prospective epidemiologic studies, randomized and controlled.
    No preview · Article · Apr 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia
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    ABSTRACT: After a short history and definition of the heterophile antibodies (antibodies in the IgM class, reacting to the antigenic determinants common to several species of animals) the paper reports on the antigens generating heterophile antibodies: the Forssman antigen, the Hanganutziu-Deicher antigen, the Paul-Brunnell antigen, respectively. Data are presented on the structure of these antigens and the important in diagnosing the heterophile antibodies in a series of diseases: malignant tumours, lymphomas, leukemias, infections mononucleosis, rheumatoid polyarthritis, Kawasaki's disease, Marek's disease.
    No preview · Article · Apr 1989 · Revista de igienă, bacteriologie, virusologie, parazitologie, epidemiologie, pneumoftiziologie. Bacteriologia, virusologia, parazitologia, epidemiologia