In vivo (Athens, Greece) (IN VIVO)

Publisher: International Institute of Anticancer Research, International Institute of Anticancer Research

Current impact factor: 0.97

Impact Factor Rankings

2016 Impact Factor Available summer 2017
2014 / 2015 Impact Factor 0.974
2013 Impact Factor 1.148
2012 Impact Factor 1.219
2011 Impact Factor 1.264
2010 Impact Factor 1.159
2009 Impact Factor 1.171
2008 Impact Factor 0.99
2007 Impact Factor 1.143
2006 Impact Factor 1.273
2005 Impact Factor 1.037
2004 Impact Factor 0.811
2003 Impact Factor 0.753
2002 Impact Factor 1.115
2001 Impact Factor 0.97
2000 Impact Factor 0.973
1999 Impact Factor 0.794
1998 Impact Factor 0.704

Impact factor over time

Impact factor
Year

Additional details

5-year impact 1.14
Cited half-life 7.10
Immediacy index 0.16
Eigenfactor 0.00
Article influence 0.24
Website In Vivo (Athens) website
Other titles In vivo (Athens, Greece: Online)
ISSN 0258-851X
OCLC 319066190
Material type Document, Periodical, Internet resource
Document type Internet Resource, Computer File, Journal / Magazine / Newspaper

Publisher details

International Institute of Anticancer Research

  • Pre-print
    • Archiving status unclear
  • Post-print
    • Author cannot archive a post-print version
  • Conditions
    • NIH Authors may deposit in PubMed Central after 4 months
  • Classification
    white

Publications in this journal

  • [Show abstract] [Hide abstract]
    ABSTRACT: Considerable evidence indicates that polyunsaturated fatty acids are important in normal brain structure and function. Rat brain striatal slices incubated with tritiated dopamine were electrically stimulated twice. During the first only buffer was perfused. During the second period buffer, fatty acid plus indomethacin, or fatty acid plus nordihydroguaiaretic acid were perfused. The ratio of the two stimulations indicated changes in released tritium. The only fatty acids to induce significant changes in tritium were the eicosanoid-precursors, dihomo-gamma-linolenic, arachidonic and eicosapentaenoic acids. There were no differences between the effects of the fatty acid alone or fatty acid in the presence of indomethacin, indicating little involvement of the cyclooxygenase pathway. Fatty acid in the presence of nordihydroguaiaretic acid reversed the low synaptic tritium concentrations, indicating that the lipoxygenase pathway may be active in dopaminergic metabolism in striatum.
    No preview · Article · Aug 2015 · In vivo (Athens, Greece)
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    ABSTRACT: Background/Aim: Interferon-alpha (IFN-alpha) is produced to act locally and transiently with a relatively short circulation half-life in vivo. Hybridization of IFN-alpha with human immunoglobulin Fc, renamed as IFN-alpha-Fc, may overcome this limitation. In the present study, I-131-IFN-alpha-Fc and I-131-IFN-alpha were compared in the aspects of stability, pharmacokinetics, tissue distribution and molecular imaging quality in an animal model. Materials and Methods: Both IFN-alpha-Fc and IFN-alpha were labelled with I-131. Biodistributions and pharmacokinetics of both labelled proteins in Sprague-Dawley rats were assayed. Micro-single-photon emission computed tomography/computed tomography was used to non-invasively monitor the longitudinal distribution of both proteins. Results: I-131-IFN-alpha-Fc was shown to have higher stability than I-131-IFN-alpha in whole blood, plasma, kidney, liver and stomach from the biodistribution study. The area under curve analyzed from plasma in the phomacokinetics study was 10-fold higher for I-131-IFN-alpha-Fc than for I-131-IFN-alpha. At 0-1 h post tail-vein injection, both labelled proteins are mainly accumulated in the kidneys and liver. Notably, I-131-IFN-alpha-Fc is degraded more slowly than I-131-IFN-alpha. Conclusion: We demonstrated that I-131-IFN-alpha-Fc has longer blood circulation time and better biostability than I-131-IFN-alpha, suggesting the potential application of the immunoglobulin Fc-conjugated cytokine for long-term treatment of diseases.
    No preview · Article · Jul 2015 · In vivo (Athens, Greece)
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    ABSTRACT: Background/Aim: Resveratrol is a polyphenol with efficient anti-oxidative and anti-inflammatory activity. To clarify the molecular mechanism responsible for its anti-inflammatory action, we investigated the radical scavenging activity, cytotoxicity and anti-inflammatory activity of resveratrol and its related compounds, orcinol and 4-allylphenol. Materials and Methods: The radical scavenging activities of these compounds were determined by the DPPH (2,2'-diphenyl-1-picrylhydrazyl) assay and their cytotoxicities against RAW264.7 cells were determined using a cell-counting kit (CCK-8). The inhibitory effects of these compounds on cyclooxygenase-2 (Cox2) expression in RAW264.7 cells stimulated with Porphyromonas gingivalis (Pg) fimbriae were also determined using real-time polymerase chain reaction and western blot analysis, while inhibition of the fimbria-stimulated activation of nuclear factor-kappa B (Nf-kappa b) was evaluated using western blot analysis and enzyme-linked immunosorbent assay-like microwell colorimetric transcription factor activity assay, respectively. The quantum chemical parameters were calculated on the basis of the density function theory (DFT) BLYP/6-31G*. Results: DPPH radical scavenging activity declined in the order resveratrol > orcinol > 4-allylphenol. The cytotoxicity of the compounds was in the order 4-allylphenol > resveratrol > orcinol. The inhibitory effect on Pg fimbria-stimulated Cox2 expression and Nf-kappa b activation was enhanced by resveratrol-alone. Resveratrol showed high electronegativity (chi) and softness (sigma) values, as determined by quantum chemical calculations. Conclusion: Resveratrol exerts potent anti-inflammatory activity in RAW264.7 cells stimulated with Pg-fimbriae and may be applicable as a therapeutic agent for inflammatory periodontal disease as a manifestation of systemic disease.
    No preview · Article · May 2015 · In vivo (Athens, Greece)
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    ABSTRACT: BACKGROUND/AIM: Greece has experienced a large wave of immigration from Bangladesh. The purpose of this study was to determine whether Bangladeshi immigrants with diabetes have poorer preventive follow-up and diabetes self-care compared to Greek patients. PATIENTS AND METHODS: A total of 166 patients from Bangladesh and 123 patients from Greece were recruited. Interviews were conducted, a physical examination followed and blood and urine samples were collected. RESULTS: Patients from Bangladesh had worse glycemic control (A1C=7.74±1.6 vs. 7.55±1.7%; p=0.3), and lower high-density lipoprotein levels (33.99±9.4 vs. 44.05±10.43 mg/dl; p=0.037). They were less likely to regularly take their medication for diabetes mellitus, or to follow dietary recommendations. However, fewer of them smoked, and had a significantly lower body mass index than Greek patients. CONCLUSION: Bangladeshi immigrants are less likely to engage self-care behaviors and have worse glycemic control and less access to medication, laboratory test and healthcare Units.
    No preview · Article · Mar 2015 · In vivo (Athens, Greece)
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    ABSTRACT: BACKGROUND: During the woman's fertile period, the non-pregnant uterus is subject to constant cyclic changes. The complex mechanisms that control the balance among proliferation, differentiation, cell death and the structural remodeling of the extracellular matrix can contribute to the benign or malignant endometrial pathological state. The small leucine-rich proteoglycans (SLRPs) are important components of cell surface and extracellular matrices. MATERIALS AND METHODS: Using immunohistochemistry, we showed that the distribution patterns of SLRPs were completely modified in the pathological compared to normal endometrium. RESULTS: The expression of SLRPs was low/absent in all endometrial pathologies examined compared to normal endometrium. We observed an increase of lumican from proliferative to secretory phase of the endometrium and a decrease of fibromodulin, biglycan and decorin. In menopause endometrial tissue, the level of expression of fibromodulin, biglycan, decorin and lumican dramatically decreased. CONCLUSION: The results revealed the prominence and importance of proteoglycans in the tissue architecture and extracellular matrix organization
    No preview · Article · Mar 2015 · In vivo (Athens, Greece)
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    ABSTRACT: The sinus sphenoidalis is the paranasal sinus most centrally located in the skull base and known to vary extremely in dimensions. A very large volume of the sinus visible on skull radiographs is called sinus 'hyperaeration'. This finding has been described many times in the literature, but is generally rare. The term 'pneumosinus dilatans' is synonymously used to address an unexpectedly large paranasal sinus. In the majority of cases, sphenoid sinus hyperaeration is not associated with any clinical symptoms. On the other hand, different complaints have been described in cases of extensive sphenoid sinus volume, such as headache, inflammation or neoplasia. This is a report of an adult patient with nasal speech who was thoroughly investigated for alterations of the upper airway in order to clarify the reason for the speech anomaly. After extensive diagnostics, the patient was found to have normal anatomy and no functional alteration of the velo-pharyngeal complex. However, an impressively large sphenoid sinus was shown on computed tomograms. This is likely the first report detailing a patient with nasal speech and hyperaeration of the sinus.
    No preview · Article · Mar 2015 · In vivo (Athens, Greece)
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    ABSTRACT: Connective tissue growth factor (CCN2) regulates diverse cellular functions, including tooth development. In order to delineate the precise role of CCN2 in the epithelium during odontogenesis, we investigated how it is expressed and what roles it may have in primary cultures of epithelial cells derived from developing tooth germ of the bovine fetus. Ccn2 mRNA and protein were strongly expressed in the inner dental epithelium, which is consistent with the expression of transforming growth factor-beta 2 mRNA and proliferating cell nuclear antigen. Bone morphogenetic protein 4 (BMP4) and fibroblast growth factor 2 (FGF2) were also expressed in the inner dental epithelium, indicating that CCN2 functionally interacts with these factors in the epithelium. The stimulatory effects of FGF2 on cell proliferation and BMP4 on cell differentiation were additively up-regulated by CCN2 in a newly-established dental epithelium cell culture. Taken together, our data provide clear evidence that CCN2 is synthesized by inner dental epithelial cells, and appears to act as an autocrine factor, which regulates dental epithelial cell proliferation and differentiation in concert with growth factors.
    No preview · Article · Mar 2015 · In vivo (Athens, Greece)
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    ABSTRACT: Background/aim: Greece has experienced a large wave of immigration from Bangladesh. The purpose of this study was to determine whether Bangladeshi immigrants with diabetes have poorer preventive follow-up and diabetes self-care compared to Greek patients. Patients and methods: A total of 166 patients from Bangladesh and 123 patients from Greece were recruited. Interviews were conducted, a physical examination followed and blood and urine samples were collected. Results: Patients from Bangladesh had worse glycemic control (A1C=7.74±1.6 vs. 7.55±1.7%; p=0.3), and lower high-density lipoprotein levels (33.99±9.4 vs. 44.05±10.43 mg/dl; p=0.037). They were less likely to regularly take their medication for diabetes mellitus, or to follow dietary recommendations. However, fewer of them smoked, and had a significantly lower body mass index than Greek patients. Conclusion: Bangladeshi immigrants are less likely to engage self-care behaviors and have worse glycemic control and less access to medication, laboratory test and healthcare Units.
    No preview · Article · Mar 2015 · In vivo (Athens, Greece)
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    ABSTRACT: MicroRNAs (miRNAs) have recently emerged as important regulators of gene expression stability. In the endometrium, miRNAs are involved in the dynamic changes associated with the menstrual cycle, implicated in implantation and in reproductive disorders. We performed a review in an attempt to assess the potential biological pathways linking altered miRNAs profiles with in vitro fertilisation (IVF) failure. Crucially, as miRNAs appear to have a significant role in the course of reproduction, they are excellent research candidates with the potential to enable a better understanding over the underlying molecular activities that prevent implantation and further progression of the embryo. Further steps include in-depth pathway mapping of the implantation process and the characterization of the respective miRNAs and associated links. The efficiency of any intervention should determine whether miRNA profiling could possibly be adopted in routine practice to substantially improve the diagnostic accuracy and, in parallel, the directed treatment of the next-generation IVF.
    No preview · Article · Mar 2015 · In vivo (Athens, Greece)
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    ABSTRACT: Background: Aberrant histone deacetylase expression may cause imbalance between acetylation and deacetylation of histone and play roles in tumor transformation. We found that histone 3 was modulated in human hepatocellular carcinoma. We determined if histone 3 modulation is related to the aberrant expression of histone deacetylase. Materials and methods: We analyzed human liver and hepatocellular carcinoma tissues and fibroblast and fibrosarcoma cell lines for the expression of histone 3, histone deacetylase 1 and acetylated histone 3 using immunohistochemistry, western blot and immunofluorescence. Results: Histone deacetylase 1 and histone 3 were more strongly detected in hepatocellular carcinoma tissue and fibrosarcoma cells than in liver tissues and fibroblast cells, respectively. However, acetylated histone 3 was more strongly expressed in normal liver and fibroblast cells and less expressed in hepatocellular carcinoma and fibrosarcoma cells. Conclusion: Histone deacetylase 1 overexpression and hypoacetylation of histone 3 might play critical roles in the modulation of histone 3 in human hepatocellular carcinoma.
    No preview · Article · Mar 2015 · In vivo (Athens, Greece)
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    ABSTRACT: Aim: The purpose of this study was to investigate the gene expression levels of elastin and fibulin-5 according to differences between carotid plaque regions and to correlate it with clinical features of plaque destabilization. Materials and methods: The study included 44 endarterectomy specimens available from operated symptomatic carotid artery stenoses. The specimens were separated according to anatomic location: internal carotid artery (ICA), external carotid artery (ECA) and common carotid artery (CCA), and then stored in liquid nitrogen. The amounts of cDNA for elastin and fibulin-5 were determined by Quantitative real-time PCR (Q-RT-PCR). Target gene copy numbers were normalized using hypoxanthine-guanine phosphoribosyltransferase (HPRT1) gene. The delta-delta CT method was applied for relative quantification. Results: Q-RT-PCR data showed that relative fibulin-5 gene expression was increased in ICA plaque regions when compared to CCA regions but not reaching significance (p=0.061). At the same time, no differences were observed in elastin mRNA level between different anatomic plaque regions (p>0.05). Moreover, elastin and fibulin-5 mRNA expression and clinical parameters were compared in ICA plaques versus CCA and ECA regions, respectively. Up-regulation of elastin and fibulin-5 mRNA levels in ICA were strongly correlated with family history of cardiovascular disease when compared to CCA (p<0.05). Up-regulation of fibulin-5 in ICA was significantly associated with diabetes, and elevated triglycerides and very low density lipoprotein (VLDL) when compared to ECA (p<0.05). Conclusion: The clinical significance is the differences between the proximal and distal regions of the lesion, associated with the ICA, CCA and ECA respectively, with increased fibulin-5 in the ICA region.
    No preview · Article · Mar 2015 · In vivo (Athens, Greece)
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    ABSTRACT: Due to the persistence and spread of antibiotic resistance, the discovery and exploitation of new antibiotic targets should be the subject of intensive research. Effective strategies are required to develop antibiotic alternatives. Antibiotics that act on new targets or via novel mechanisms have the greatest likelihood of overcoming resistance. In particular, there is a lack of specific antibiotics for Pseudomonas aeruginosa, one of the leading causes of healthcare-associated infections, exhibiting high resistance levels. Herein we describe how structure-based drug design can be used to achieve new antibiotics for the treatment of Pseudomonas aeruginosa infection, using an essential enzyme of the fatty acid synthesis pathway from P. aeruginosa as an example.
    No preview · Article · Mar 2015 · In vivo (Athens, Greece)
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    ABSTRACT: Background/Aim: Claudins (CLDNs) are crucial structural and functional components of tight junctions playing an important role in maintaining cell polarity, controlling paracellular diffusion and regulating cell growth and differentiation of epithelial cells. CLDNs are differentially expressed in neoplastic lesions compared to the corresponding healthy tissues; therefore, they are thought to play a role in tumorigenesis and tumor progression. Reduced expression of CLDN-1 has been observed in several types of human cancers, including thyroid tumors. There are no reports of CLDN-1 immunoexpression in normal and neoplastic canine thyroid tissues. Material and Methods: CLDN-1 immunoexpression was investigated in normal canine thyroid gland (n=2), benign (n=1) and malignant (n=10) tumors, as well as neoplastic emboli (n=6). Results: CLDN-1 was constitutively expressed in normal canine follicular epithelium. Ninety percent of the malignant canine thyroid lesions showed absence or reduced CLDN-1 expression compared to that of normal thyroid gland. Additionally, a cytoplasmic subcellular location of CLDN-1 was recorded in the malignant epithelial cells and neoplastic emboli. Conclusion: These findings link altered expression of CLDN-1 to neoplastic transformation and suggest that CLDN-1 expression is associated with malignant canine thyroid tumors and their vascular invasion. Copyright © 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
    No preview · Article · Jan 2015 · In vivo (Athens, Greece)
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    ABSTRACT: Lung cancer is the leading cause of cancerrelated deaths and new lung cancer cases are continuously emerging around the globe; however, treatment of lung cancer remains unsatisfactory. Demethoxycurcumin (DMC) has been shown to exert cytotoxic effects in human cancer cells via induction of apoptosis. However, the effects of DMC on genetic mechanisms associated with these actions have not been yet elucidated. Human lung cancer NCI-H460 cells were incubated with or without 35 μM of DMC for 24 h and total RNA was extracted for cDNA synthesis labeling and microarray hybridization, followed by fluor-labeled cDNA hybridization on chip. Expression Console software with default Robust Multichip Analysis (RMA) parameters were used for detecting and quantitating the localized concentrations of fluorescent molecules. The GeneGo software was used for investigating key genes involved and their possible interaction pathways. Genes associated with DNA damage and repair, cell-cycle check point and apoptosis could be altered by DMC; in particular, 144 genes were found up-regulated and 179 genes down-regulated in NCI-H460 cells after exposure to DMC. In general, DMC-altered genes may offer information to understand the cytotoxic mechanism of this agent at the genetic level since gene alterations can be useful biomarkers or targets for the diagnosis and treatment of human lung cancer in the future.
    No preview · Article · Jan 2015 · In vivo (Athens, Greece)