University of the Azores

Objectives to identify and summarize the elements that characterize the family transition process in relation to the first child. Methods a scoping review was carried out based on JBI methodology, in six databases, following the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews checklist. Results ten articles were included with factors characterizing the transition, such as hindering/facilitating conditions that influence the process, important support structures in adaptation and strategies/responses used in the transition process. Final Considerations elements characterizing the transition process in relation to the first child were identified. However, no theoretical explanation for this was identified. Further research should be carried out to obtain a deeper understanding of this process. Descriptors: Transition; Family; Parenthood; First Child; Nursing

Objectives to identify and summarize the elements that characterize the family transition process in relation to the first child. Methods a scoping review was carried out based on JBI methodology, in six databases, following the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews checklist. Results ten articles were included with factors characterizing the transition, such as hindering/facilitating conditions that influence the process, important support structures in adaptation and strategies/responses used in the transition process. Final Considerations elements characterizing the transition process in relation to the first child were identified. However, no theoretical explanation for this was identified. Further research should be carried out to obtain a deeper understanding of this process. Descriptors: Transition; Family; Parenthood; First Child; Nursing

Since their domestication, horses have accompanied mankind, and humans have constantly shaped horses according to their needs through stallion-centered breeding. Consequently, the male-specific portion of the Y chromosome (MSY) is extremely uniform in modern horse breeds. The majority of stallions worldwide carry MSY haplotypes (HT) attributed to an only ~1,500-y-old, so-called, “Crown” haplogroup. The predominance of the Crown in modern horse breeds is thought to represent a footprint of the vast impact of stallions of “Oriental origin” in the past millennium. Here, we report the results of a fine-scaled MSY haplotyping of large datasets of patrilines comprising 1,517 males of 189 modern horse breeds, covering a broad phenotypic and geographic spectrum. We can disentangle the multilayered influence of Oriental stallions over the last few hundred years, exposing the intense linebreeding and the wide-ranging impact of Arabian, English Thoroughbred, and Coldblood sires. Iberian and New World horse breeds contain a wide range of diversified Crown lineages. Their broad HT spectrum illustrates the spread of horses of Oriental origin via the Iberian Peninsula after the Middle Ages, which is commonly referred to as the “Spanish influence.” Our survey also revealed a second major historical dissemination of horses from Western Asia, attributed to the expansion of the Ottoman Empire. Our analysis shows that MSY analysis can uncover the complex history of horse breeds and can be used to establish the paternal ancestry of modern horse breeds.

Alzheimer’s disease (AD), a multifactorial neurodegenerative disorder characterized by severe cognitive impairment, affects millions of people worldwide. However, AD therapy remains limited and mainly symptomatic-focused, with acetylcholinesterase (AChE) inhibitors being the major available drugs. Thus, AD is considered by the WHO as a disorder of public health priority. Among several strategies that have been identified to combat AD, the use of natural multi-target drug ligands (MTDLs) appears to be a promising approach. In this context, we previously found that the essential oils (EOs), obtained via hydrodistillation, from Azorean Cryptomeria japonica sawdust (CJS) and resin-rich bark (CJRRB) were able to exert antioxidant activity via different mechanisms of action. Therefore, in the present work, these EOs were screened for their (i) in vitro anti-AChE and anti-butyrylcholinesterase (BChE) activities, evaluated by a modified Ellman’s assay; (ii) in vitro anti-inflammatory potential, using the albumin denaturation method; and (iii) toxicity against Artemia salina. The CJRRB–EO exhibited both anti-AChE and anti-BChE activities (IC50: 1935 and 600 µg/mL, respectively), whereas the CJS–EO only displayed anti-BChE activity, but it was 3.77-fold higher than that of the CJRRB–EO. Molecular docking suggested that α-pinene and ferruginol compounds contributed to the anti-AChE and anti-BChE activities, respectively. Moreover, the anti-inflammatory activity of the CJS–EO, the CJRRB–EO, and diclofenac was 51%, 70%, and 59% (at a concentration of only 2.21 μg/mL), respectively, with the latter two presenting comparable activity. Concerning the EOs’ potential toxicity, the CJRRB–EO exhibited a lower effect than the CJS–EO (LC50: 313 and 73 µg/mL, respectively). Overall, the EOs from C. japonica biomass residues, chiefly the CJRRB–EO, displayed antioxidant, anticholinesterase, and anti-inflammatory activities in a concentration-dependent manner. These properties demonstrate that these residues may be suitable natural MTDLs for AD complementary therapy when administered through aromatherapy, or, alternatively, could serve as low-cost sources of valuable ingredients, such as α-pinene.

Physalia physalis, commonly known as the Portuguese Man o’ War, is one of the most venomous members of the Cnidaria yet is poorly understood. This article investigates the toxicity of P. physalis venom by assessing its behavioral and toxicological effects on Drosophila melanogaster. The venom administered orally revealed dose- and time-dependent mortality, with an LD50 of 67.4 µg per fly. At sublethal doses, the treated flies displayed uncoordinated movement and fell when attempting to climb. Real-time analysis of flies exposed to the venom revealed hyperexcitability followed by paralysis, with phenotypes similar to those observed in vertebrate models. The venom was shown to be non-thermolabile, as no significant differences in behavior and locomotion were observed between flies exposed to untreated or thermally treated venom. The circadian rhythm alterations, the enhanced light a raction, and the reduction in heat avoidance suggest altered neuronal function. This abnormal behavior indicates that the venom contains bioactive molecules, opening avenues for discovering new compounds with potential for pharmacological applications.

Aim: The Neogene fossil record of Pinus canariensis C.Sm ex DC. suggests a wider past distribution in Europe, but due to extirpation by past climatic events, today this taxon is restricted to the Canary Islands. Remarkably, the putative oldest P. canariensis fossil for the Canary Islands, found in Gran Canaria is 13.3–13.0 Ma. This palaeobiogeographical information has been cited as proof of the deep-time presence of conifers, the genus Pinus L., P. canariensis and the pine forest ecosystems in the Canary Islands, but also to justify the long-term evolution of P. canariensis within an active insular volcanic environment. Here we present a re-evaluation of the oldest Pinus fossil from the Canary Island. Location: Gran Canaria, Canary Islands, Spain. Taxon: Pinus canariensis C.Sm ex DC. Methods: We compiled a citation record for the mentions of the ‘bark fossil’ and conducted a review of the specimen. We compared it with extant P. canariensis and with pyroclasts. Results: The analysis of the specimen demonstrates that it lacks morphological or anatomical characters to identify as a bark or even as a plant fossil. The specimen is best interpreted as a lapilli sized tube pumice, highly altered by mineral deposition promoted by the percolating hydrothermal water. Main Conclusions: The oldest reliable Pinus fossils from the Canary Islands are 3.9–3.1 Ma and ca. 9 to 10 Ma younger than previously claimed. When did Pinus arrive at the Canary Islands is unknown, but this biogeographical question can be approached via focused volcano-stratigraphical and palaeobotanical research in this Archipelago.

Corals and gorgonians play an important role as ecosystem engineers in many sublittoral communities worldwide. However, coral populations are being increasingly impacted by human activities. Active ecological restoration is a popular conservation tool nowadays, widely used to revert degradation in natural populations. Several studies have reported survival and growth success in actively restored corals, but few have studied the long‐term effect of transplantation on coral reproduction. We investigated survival and sexual reproduction of transplanted colonies of the Mediterranean gorgonian Eunicella singularis up to 3 years after being recovered from fishery bycatch and transplanted back to their habitat. Three different colony sizes were tested to explore possible effects of fragmentation on survival and reproduction. Results showed that transplanted colonies suffered a significant mortality during the first 4–5 months (30–35%), independently of fragment size. However, larger fragments showed high survival despite other disturbances, as a bloom of ephemerons filamentous algae occurred during the study. Results also showed that reproductive capacity is negatively affected by transplantation in female colonies. Conversely, male colonies were not directly affected by transplantation but showed an unexpectedly reduced number of spermary sacs both in transplanted and natural control colonies. The trade‐off between investment in reproduction and mortality of transplants highlights that large colony size could be the most appropriate for the active restoration of E. singularis . This study emphasizes the need to explore the long‐term viability and the reproduction investment in transplanted corals as indicators of restoration success, whose populations are sustained by sexual reproduction.

The knowledge on taxonomic diversity of arthropods is key to better understanding the biodiversity patterns and processes and guiding sustainable conservation strategies and practices. In the Azores, terrestrial arthropods are relatively well-inventoried following the publication of comprehensive checklists that have been regularly updated. Nevertheless, every year, new species are found as a result of new arrivals to the Archipelago and from addressing specific taxonomic lacunae. Here, we update the taxonomic terrestrial arthropod biodiversity of the Azores by reporting for the first time 13 species for the Archipelago, namely Oligonychus perseae Tuttle, Baker & Abbatiello, 1976, Textrix pinicola Simon, 1875, Pholcomma gibbum (Westring, 1851), Schistocerca gregaria (Forsskål, 1775), Phoracantha recurva Newman, 1840, Diachus auratus Fabricius, 1801 Phyllotreta procera (Redtenbacher, 1849), Phyllotreta striolata (Fabricius, 1803), Dibolia occultans (Koch, 1803), Pseudolynchia canariensis (Macquart, 1839), Hermetia illucens (Linnaeus, 1758), Dryocosmus kuriphilus Yasumatsu, 1951 and Ectemnius cephalotes (Olivier, 1792), and several new species records for specific islands. These species benefitted from the increase in transportation of goods and commodities, both from outside the Archipelago and between islands, to arrive and spread across the Archipelago with some of them posing new challenges to local agriculture, forestry and biodiversity conservation management.

Aim We map global patterns of taxonomic and functional change between past (pre‐human impacts) and present (after anthropogenic extinctions and introductions) in large oceanic island bird assemblages and investigate if these patterns can be explained by island characteristics and anthropogenic factors. Location Sixty‐four oceanic islands (>100 km ² ). Time Period Late Holocene. Major Taxa Studied Terrestrial and freshwater bird species. Methods We compiled information on extinct, extant native and introduced bird species for all islands and used a probabilistic hypervolume approach to build a multi‐dimensional trait space and calculate several functional diversity metrics before and after extinctions and introductions. We identified which islands are global hotspots of human‐induced transformation by mapping multiple facets of biotic change and investigated intrinsic island characteristics and anthropogenic factors as drivers for these observed patterns. Results The Hawaiian and Mascarene islands stand out as hotspots of taxonomic and functional change, but all islands changed taxonomically and functionally, mostly gaining species but losing functional richness. Taxonomic and functional changes vary across islands but are often consistent within the same archipelago. Island isolation and surface can explain some of the observed variations, but anthropogenic factors, namely human occupation, also shaped both taxonomic and functional changes. Islands with higher human pressure, as well as larger islands with high elevation ranges, tended to have greater losses in functional richness. Main Conclusions Most biodiversity change assessments are still largely based exclusively on taxonomic diversity, which is particularly worrying in the case of oceanic islands given that the magnitude of functional diversity change is often considerably larger. We call for comprehensive assessments of changes in both taxonomic and functional diversity across oceanic islands in order to better understand the drivers of these changes and, in turn, predict future trends.

Styrylchromones (SC) are a group of oxygen‐containing heterocyclic compounds, which are characterized by the attachment of a styryl group to the chromone core. SC can be found in nature or can be chemically synthesized in the laboratory. As their presence in nature is scarce, the synthetic origin is the most common. Two types of SC are known: 2‐styrylchromones and 3‐styrylchromones. However, 2‐styrylchromones are the most common, being more commonly found in nature and which chemical synthesis is more commonly described. A wide variety of SC has been described in the literature, with different substituents in different positions, the majority of which are distributed on the A‐ and/or B‐rings. Over the years, several biological activities have been attributed to SC. This work presents a comprehensive review of the biological activities attributed to SC and their structure‐activity relationship, based in a published literature search, since 1989. The following biological activities are thoroughly revised and discussed in this review: antioxidant, antiallergic, antiviral, antibacterial, antifungal, anti‐inflammatory and antitumoral, affinity and selectivity for A3 adenosine receptors, neuroprotective, and α‐glucosidase inhibition. In general, SC are composed by a promising scaffold with great potential for the development of new drugs.

Examining ecosystem functioning through the lens of trait diversity serves as a valuable proxy. It offers crucial insights into how exploitation affects the specific ecological roles played by fisheries targeted species. The present study investigates the potential impacts of exploitation on the ecological roles of fish species targeted by fisheries through an examination of trait diversity. It focuses on the trait diversity of fish landed by local and coastal fleets in the Azores archipelago over the past four decades. Fourteen functional traits were merged to data on fish assemblages landed by both fishing fleets from 1980 to 2020. These traits corresponded to four fundamental fish functions: habitat use, locomotion, feeding and life history. Variability in functional diversity metrics (i.e., functional richness- FRic, functional evenness- FEve, functional divergence-FDiv, and functional dispersion- FDis) among fleets, functions and across decades was assessed using null models. The results revealed similar trait diversity between assemblages landed by local and coastal fishing fleets with overall trait diversity remaining relatively stable over time. However, fishery activities targeted a wide range of functional traits. Additionally, seasonal availability and increased catches of certain fish species can significantly alter trait diversity and their associated functions. The findings highlight the importance of addressing fishing impacts on species traits and their ecological roles, which is crucial for long-term fisheries and ecological sustainability.

The study of larval dispersal and connectivity between deep-sea populations is essential for the effective conservation and management of deep-sea environments and the design and implementation of Marine Protected Areas. Dense sponge aggregations, known as “sponge grounds”, are a key component of marine benthic ecosystems, by increasing the structural complexity of the sea floor and providing structure and habitat for many other species. These aggregations are characteristic of the Azores deep-sea environment. These sessile organisms rely primarily on larval dispersal for their reproduction. Connectivity between specific Pheronema carpenteri sponge aggregations in the Azores was studied using a 3-D biophysical dispersal model. Different biological trait scenarios were analyzed, considering spawning seasonality and pelagic larval duration. Model results indicate that regional circulation patterns drive larval dispersion, shaping population connectivity of P. carpenteri sponge aggregations in the Azores, particularly among aggregations in the Central Group of Azorean islands. Some areas present high retention rates, receiving larvae from several sponge aggregations while also being important larval source aggregations. In contrast, aggregations from the Eastern Group may be isolated from the others. Larval dispersal and connectivity patterns were analyzed concerning the current configuration of Marine Protected Areas (MPAs) in the Azores. The results underscored the importance of maintaining protection efforts in existing MPAs and identified stepping-stone locations and specific sites where additional measures could enhance species connectivity in the Azores.

Natural compounds are widely incorporated into cosmetic products for many purposes. Diterpenes often function as fragrances, enhancing the sensory experience of these formulations. However, current trends in cosmetic science aim to develop multifunctional products, where compounds traditionally used for texture or fragrance also possess biological activities that contribute to the product’s efficacy. In this context, this study focuses on synthesizing derivatives of phytol—a compound already presents in cosmetic formulations—to enhance its anti-aging properties. The derivatives were synthesized through esterification with substituted benzoic and cinnamic acids, known for their antioxidant and enzyme inhibition properties. Reaction yields ranged from 91.0% to 5.2%, depending on the substituents in acid derivatives. The structures of the synthesized compounds were confirmed through NMR and MS techniques. Both the natural and newly synthesized derivatives were evaluated for their cosmeceutical potential using antioxidant assays and inhibition assays for tyrosinase, elastase, collagenase, and hyaluronidase. This work presents the first report of the synthesis and cosmetic evaluation of several of these derivatives. Comparing with phytol (1), which presented an IC50 of 77.47 µM, four of the derivatives presented improved tyrosinase inhibitory activity, with phytyl 4-methoxybenzoate being the most active (IC50 = 27.9 µM), followed by phytyl benzoate with an IC50 of 34.73 µM. Substitutions at other positions on the aromatic ring were less effective. Molecular docking studies confirmed that the modifications potentiated a stronger interaction between the synthesized compounds and tyrosinase.

Background The EORTC Quality of Life Group has developed a questionnaire to evaluate cancer patients’ perception of their communication with healthcare professionals (HCPs): the EORTC QLQ-COMU26. In this study we test the validity and reliability of this novel measure in an international and culturally diverse sample of cancer patients. Methods Cancer patients completed the following EORTC questionnaires at two time points (before and during treatment): the QLQ-COMU26 (including a debriefing questionnaire), the QLQ-C30, and specific IN-PATSAT32 scales. These data were used to assess: the cross-cultural applicability, acceptability, scale structure, reliability, convergent/divergent validity, known-groups validity, and responsiveness to change of the QLQ-COMU26. Results Data were collected from 498 patients with various cancer diagnoses in 10 European countries, Japan, Jordan and India (overall 5 cultural regions). At most, only 3% of patients identified an item as confusing and 0.6% as upsetting, which indicates that the questionnaire was clear and did not trigger negative emotional responses. Confirmatory factor analysis and multi-trait scaling confirmed the hypothesised QLQ-COMU26 scale structure comprising six multi-item scales and four single items (RMSEA = 0.025). Reliability was good for all scales (internal consistency > 0.70; test–retest reliability > 0.85). Convergent validity was supported by correlations of ≥ 0.50 with related scales of the IN-PATSAT32 and correlations < 0.30 with unrelated QLQ-C30 scales. Known-groups validity was shown according to sex, education, levels of anxiety and depression, satisfaction with communication, disease stage and treatment intention, professional evaluated, and having a companion during the visit. The QLQ-COMU26 captured changes over time in groups that were defined based on changes in the item of satisfaction with communication. Conclusion The EORTC QLQ-COMU26 is a reliable and valid measure of patients’ perceptions of their communication with HCPs. The EORTC QLQ-COMU26 can be used in daily clinical practice and research and in various cancer patient groups from different cultures. This questionnaire can help to improve communication between patients and healthcare professionals.

The first insights on habitat and phylogenetic origin of a newly found population of Solen marginatus are provided in the mid-North Atlantic Azores archipelago, in the bay of Praia da Vitória, Terceira Island. Distribution is confined to the northern portion and most sheltered part of the bay down to 14.2 m depth. Densities with an average of 12.69 individuals/m ² were found at 8.4 m depth, using 4 × 20 sqm visual transects. Sizes of shell length between 10 and 12 cm comprised 60% of collected specimens ( n = 118), ranging between 8.79 and 15.4 cm and averaging 11.28 cm. Considering shell length, the high densities and dispersion area, a settlement period above 20 years is estimated. Greater genetic affinity was found in the Ria de Aveiro (North of Portugal) and the Asturias populations (North of Spain). The source origin remains undetermined, with intentional or non-intentional anthropogenic introduction, as well as natural dispersion remaining possible, although more unlikely. Due to the commercial value of this species, a new clam fishery is likely to develop in the area, requiring further studies and immediate conservation measures.

Assessment is a crucial aspect of music performance. In pedagogical contexts, an effective assessment process can measure student achievement and inform instructional decisions that contribute to improving teaching and learning. However, music performance assessment is particularly challenging due to its inherent subjectivity, involving personal expression and interpretation, which can lead to divergent opinions. In this PRISMA systematic review (registration DOI: 10.17605/OSF.IO/CSM8Q), we aimed to delimit and analyze solo music performance assessment systems found in the literature to date, including their corresponding evaluation categories and descriptive criteria, rating methodology, and target audience. A search in three main scientific databases (Web of Science, Scopus, ERIC) was conducted using keywords associated with the topic of assessment in the field of solo music performance. Ultimately, 20 papers were selected and examined, resulting in 26 original assessment systems for analysis. Regarding sample characteristics, we found that studies mainly focused on evaluating high school and university students, with music teachers and faculty members serving as primary evaluators. Many assessment systems were designed to be applicable across various instruments, although some were tailored to specific instruments (e.g., piano, voice) and families (e.g., brass, woodwind). Systems typically structured evaluation around technical, interpretative/expressive, and various musical feature categories (e.g., pitch, rhythm, intonation), further elaborated with descriptive items. While five-point Likert scales were commonly used, recent studies indicated a shift towards rubrics for detailed feedback, which aids examiners’ understanding and supports student progress. No differentiation was found in assessment criteria based on students’ learning stages, suggesting an area for improvement in refining these assessment methods. This study identifies gaps and proposes improvements in existing assessment systems, providing a foundation for educators and policymakers to enhance curriculum design and instructional practices in music education.

Introduction Socio-emotional security and particularly secure attachment relationships with parents and peers have been associated with positive developmental outcomes, including school-related variables. This systematic literature review aims to provide a comprehensive analysis of the literature, exploring the relationship between socio-emotional security (attachment, support, and secure relations with parents and peers) and academic achievement, school engagement, and early school dropout. Methods The inclusion criteria were studies with socio-emotional security as the independent variable and academic achievement and/or school engagement as the dependent variable, using a quantitative methodology, written in English. The exclusion criteria were articles presenting literature review or meta-analysis, interventions or instrument validation studies, studies with a qualitative approach, studies developed in an e-learning context, studies with university students, and/or focused on schooling in extreme conditions (e.g., COVID-19). PRISMA guidelines were followed, through a search that resulted in the identification of 38 empirical quantitative studies, published between 2018 and 2022, in English. Results and discussion The results revealed that parent and peer relationships impact students’ academic achievement and school engagement, as expected, and highlighted the relevance of parental and peer relationships for school-related outcomes. Future research should consider the role of potential mediators and moderators in the relationship between socio-emotional security and school outcomes.

Spinocerebellar ataxia type 3/Machado-Joseph disease (SCA3/MJD) is caused by the expansion of a genetically unstable polyglutamine-encoding CAG repeat in ATXN3. Longer alleles are generally associated with earlier onset and frequent intergenerational expansions mediate the anticipation observed in this disorder. Somatic expansion of the repeat has also been implicated in disease onset and slowing the rate of somatic expansion has been proposed as a therapeutic strategy. Here, we utilised high-throughput ultra-deep MiSeq amplicon sequencing to precisely define the number and sequence of the ATXN3 repeat, the genotype of an adjacent single nucleotide variant and quantify somatic expansion in blood and buccal swab DNA of a cohort of individuals with SCA3 from the Azores islands (Portugal). We revealed systematic mis-sizing of the ATXN3 repeat and high levels of inaccuracy of the traditional fragment length analysis that have important implications for attempts to identify modifiers of clinical and molecular phenotypes. Quantification of somatic expansion in blood DNA and multivariate regression revealed the expected effects of age at sampling and CAG repeat length, although the effect of repeat length was surprisingly modest with much stronger associations with age. We also observed an association of the downstream rs12895357 single nucleotide variant with the rate of somatic expansion, and a higher level of somatic expansion in buccal swab DNA compared to blood. These data suggest that the ATXN3 locus in SCA3 patients in blood or buccal swab DNA might serve as a good biomarker for clinical trials testing suppressors of somatic expansion with peripheral exposure.