University of Eastern Finland

Organic raw materials are the renewable sources of substrates for our industries and for our microbial communities. As industrial, agricultural or forestry side streams, they are usually affordable if the process entities, equipment and protocols are properly designed. The microbial communities that are used as biocatalysts take care of the process development together with us or with the process team. Moreover, they constitute or shape the process to resemble the natural bioprocess as it takes place or occurs in nature and thus make it “Industry Like Nature®” – type of endeavor. As an ultimate result, we could make our industries increasingly 100% sustainable with the help of microbes. In case of food or forest industry side streams, this means fossil-free production of valuable chemicals, food and feed components, energy and gases, and soil improvement or organic fertilizers. The so-called “Finnoflag biorefinery” idea has been tested in many cases together with domestic and international colleagues and industries. In here, we attempt to share the basic thinking.

Background The prevalence of vision-threatening diseases, such as age-related macular degeneration (AMD) and diabetic macular edema (DME), is likely to increase in developed countries owing to an aging population, rising life expectancy, and unfavorable lifestyle changes. Increases in the burden of vision-threatening diseases pose a challenge to the healthcare system. After the emergence of intravitreal anti-VEGF inhibitors, treatment options for neovascular AMD (nAMD), DME, retinal vein occlusion (RVO) and myopic choroidal neovascularization (myopic CNV) have increased. As this change in treatment practices has occurred over the last two decades, it is important to demonstrate changes in patient numbers and administered treatments to provide solutions for handling the workload and productivity in ophthalmology departments. In addition, the registry data landscape has evolved in Finland in recent years. Thus, understanding the possibilities and limitations of ophthalmology registries and patient information systems is required. Methods This study involved the secondary use of retrospectively registered data from the data warehouse of the Hospital District of Southwest Finland. Our goal was to explore how the workload of ophthalmology departments caused by intravitreal injections has evolved from 2015 to 2022. Results The ophthalmology department workload increased significantly during our observation period as the total number of patients receiving intravitreal treatments for nAMD, DME, RVO, and myopic CNV increased 199.6% from 2015 to 2021. In addition, the total number of administered anti-VEGF injections increased during our observation period, but the increase rate began to subside (2019–2020: increase 23.7%, 2020–2021: increase 10.3%, 2021–2022: increase 6.7%). Conclusion Supporting the utilization of registry data is essential in evidence-based discussions evolving workload in healthcare. However, it is important to understand the limitations and the quality of the registries. Our study contributes to better understanding the Finnish registry perspective, and it demonstrates the increase in workload in ophthalmology departments caused by intravitreal injections.

Finland has adopted a comprehensive approach towards national security set out in the Government Resolution on Security Strategy for Society (2017). Put succinctly in the Security Strategy, “Comprehensive security is the Finnish cooperation-based preparedness model, in which the vital functions of society are jointly managed by the authorities, business operators, organisations and citizens” (Security Strategy for Society 2017). The Strategy—like all Government Resolutions—is a guiding document and presents the outlines for national preparedness activities and the division of responsibilities, as well as gives general guidance on legislation. A comprehensive approach to security emphasises the idea of whole-of-society participation in sharing the burden of crisis preparedness. This means that all actors (authorities, business operators, citizens, and other organisations) must ensure an adequate level of preparedness in their daily operations.

Chronic rhinosinusitis (CRS) is a clinical syndrome defined by typical sinonasal symptoms persisting for at least 12 weeks. CRS is divided into two distinct phenotypes, CRS with nasal polyps (CRSwNP) and without (CRSsNP). The aim of the review is to provide an update on the current knowledge in CRS endotypes. The prevailing hypothesis regarding the pathogenesis of CRS suggests that dysfunctional interactions between the host and environmental stressors at the mucosal surface drive the diverse inflammatory mechanisms. Genetic and epigenetic variations in the mucosal immune system are believed to play a significant role in the pathomechanisms of CRS. Various environmental agents (such as microbes and irritants) have been implicated in CRS. In a healthy state, the sinonasal mucosa acts as a barrier, modulating environmental stimulation and mounting appropriate immune responses against pathogens with minimal tissue damage. Different endotypes may exist based on the specific mechanistic pathways driving the chronic tissue inflammation of CRS. There is a need to understand endotypes in order to better predict, diagnose, and treat CRS. This literature review provides an update on the role of the endotypes in CRS and the limitations of endotyping CRS in clinical practice. Understanding of the pathogenesis and optimal management of CRS has progressed significantly in the last decades; however, there still are several unmet needs in endotype research.

Colorectal cancer (CRC) is one of the most common cancers worldwide. The standard CRC chemo drug, 5-Fluorouracil (5-FU), has a poor response rate and chemoresistance, prompting the need for a more effective and affordable treatment. In this study, we aimed to evaluate whether Prohep, a novel probiotic mixture, would alleviate azoxymethane/dextran sodium sulfate (AOM/DSS)-induced colorectal tumorigenesis and enhance 5-FU efficacy and its mechanism. Our results suggested that Prohep showed stronger anti-tumorigenesis effects than 5-FU alone or when combined in the AOM/DSS model. Prohep significantly reduced the total tumor count, total tumor size, caecum weight, colonic crypt depth, colonic inflammation, and collagen fibrosis. Prohep downregulated pro-inflammatory TNF-α and proliferative p-STAT3 and upregulated apoptotic p53. Metagenomics analysis indicated that Prohep-enriched Helicobacter ganmani, Desulfovibrio porci, Helicobacter hepaticus, and Candidatus Borkfalkia ceftriaxoniphila were inversely correlated to the total tumor count. In addition, Prohep-enriched Prevotella sp. PTAC and Desulfovibrio porci were negatively correlated to AOM/DSS enriched bacteria, while forming a co-existing community with other beneficial bacteria. From KEGG analysis, Prohep downregulated CRC-related pathways and enhanced pathways related to metabolites suppressing CRC like menaquinone, tetrapyrrole, aminolevulinic acid, and tetrahydrofolate. From Metacyc analysis, Prohep downregulated CRC-related peptidoglycan, LPS, and uric acid biosynthesis, and conversion. Prohep elevated the biosynthesis of the beneficial L-lysine, lipoic acid, pyrimidine, and palmitate. Prohep also elevated metabolic pathways related to energy utilization of lactic acid-producing bacteria (LAB) and acetate producers. Similarly, fecal acetate concentration was upregulated by Prohep. To sum up, Prohep demonstrated exceptional anti-tumorigenesis effects in the AOM/DSS model, which revealed its potential to develop into a novel CRC therapeutic in the future. Graphical Abstract

The Italian Po Valley is one of the most polluted regions in Europe. During winter, meteorological conditions favor long and dense fogs, which strongly affect visibility and human health. In spring, the frequency of nighttime fogs reduces while daytime new particle formation events become more common. This transition is likely caused by a reduction in PM 2.5 , leading to a decrease in the relevant condensation sink. The physics and chemistry of fog and aerosol have been studied at the San Pietro Capofiume site since the 1980s, but the detailed processes driving the observed trends are not fully understood. Hence, during winter and spring 2021/22, the Fog and Aerosol InteRAction Research Italy (FAIRARI) campaign was carried out, using a wide spectrum of approaches, including in-situ measurements, outdoor chamber experiments, and remote sensing. Atmospheric constituents and their properties were measured ranging from gas molecules and molecular clusters to fog droplets. One unique aspect of this study is the direct measurement of the aerosol composition in- and outside of fog, showing a slightly greater dominance of organic compounds in the interstitial compared to the droplet phase. Satellite observations of fog provided a spatial context and agreed well with in-situ measurements of droplet size. They were complemented with in-situ chamber experiments, providing insights into oxidative processes and revealing a large secondary organic aerosol forming potential of ambient air upon chemical aging. The oxidative potential of aerosol and fog water inferred the impact of aerosol-fog interactions on particle toxicity.

Purpose Psychological distress has been associated with sickness absence (SA), but less is known about whether there are distinct patterns in the development of SA among people with psychological distress. We examined trajectories of short- and long-term SA among employees with psychological distress and how social and health-related factors are associated with them. Methods We used the employer’s register data on all-cause short- (≤ 10 working days) and long-term (> 10 working days) SA with a two-year follow-up. We prospectively linked the Helsinki Health Study survey data on 19–39-year-old employees of the City of Helsinki, Finland, in 2017, to the SA data. We included 1060 participants (81% women) who reported experiencing psychological distress, measured by the emotional wellbeing scale of RAND-36. Survey responses of age; gender; education; marital status; social support, procedural and interactional organisational justice, and bullying at work; physical activity; diet; tobacco and alcohol use; prior SA; and the level of psychological distress were included as exposures. Group-based trajectory modelling and multinomial logistic regression were used for the analyses. Results We identified four short-term SA trajectories: ‘low’ (n = 379, 36% of participants), ‘descending’ (n = 212, 20%), ‘intermediate’ (n = 312, 29%), and ‘high’ (n = 157, 15%); and two long-term SA trajectories: ‘low’ (n = 973, 92%) and ‘high’ (n = 87, 8%). A higher education, fewer prior SA, and lower levels of psychological distress were associated with the ‘low’ short- and long-term SA trajectories. Conclusion SA trajectories differ among employees with psychological distress. Early intervention and support are needed among employees with mental health symptoms to prevent future SA.

Over the past three decades, incidental bycatch has been the single most frequent verified cause of death of the endangered Saimaa ringed seal (Pusa hispida saimensis). Spatial and temporal fishing closures have been enforced to mitigate bycatch, which is mainly caused by the gillnets of recreational fishers. In this study, we employed an array of statistical machine learning methods to recognize patterns of death and to evaluate the impacts of annual fishing closures (15th April–30th June) on the recovery of the Saimaa ringed seal population during 1991–2021. We additionally used the potential biological removal (PBR) procedure to assess bycatch sustainability. The study shows that gillnet restriction areas are reflected in the timing of juvenile bycatch mortality of the Saimaa ringed seal. In the 1990s, peak mortality occurred at the beginning of June, but as the restrictions expanded regionally in the 2000s, the peak shifted to the beginning of July. Longer temporal coverage of annual closures would have improved juvenile survival. The study also shows that estimated bycatch mortality is higher than observed: the estimated bycatch averaged approximately two unobserved bycatches per one observed bycatch. Despite the continuing bycatch mortality, a larger number of juveniles nowadays survive to the age of 15 months due to fishing closures, and the population (some 420 individuals) has increased an average 4% per year between 2017 and 2021. However, human-caused mortality limits (PBR) were exceeded by observed bycatch only, which could lead to population depletion in the long run.

Objective We analyzed how anthropometric measures predict cardiometabolic health and how genetic and environmental factors contribute to these associations. Methods Data on 8 indicators of cardiometabolic health, 21 anthropometric measures, and 11 anthropometric indices were available for 216 twin pairs of individuals age 3 to 18 years living in the Autonomous Region of Madeira, Portugal (51% girls). Genetic twin modeling was used to estimate genetic and environmental correlations between the cardiometabolic and anthropometric indicators. Results Anthropometric indicators were positively associated with blood pressure and triglycerides and inversely associated with high‐density lipoprotein cholesterol. The associations with glucose, low‐density lipoprotein cholesterol, total cholesterol, and heart rate were close to zero. BMI and waist circumference showed similar or slightly higher absolute correlations with cardiometabolic health indicators compared with other anthropometric indices. Additive genetic and unique environmental correlations were at the same level as trait correlations. Conclusions BMI and waist circumference provide information on cardiometabolic health that is not less accurate than that provided by more comprehensive anthropometric indices. These associations reflect causal associations between obesity and cardiometabolic disorders rather than only shared genetic associations. Measuring obesity is important for monitoring cardiometabolic risks and can be accomplished using simple indicators at the population level.

Objective The purpose of this study is to examine how care workers’ characteristics are related to the perceived benefits and drawbacks of using videoconferencing in the care of older people. The factors chosen for this study are: age, education, perceived information and communication technology (ICT) support, interest in technology, ICT skills and possible prior experience of using videoconferencing tools. Methods Our data source was the second wave (2021) of the University of Jyväskylä survey on elder care work (N = 3607), collected from four large trade unions in Finland. We used a multinomial logistic regression to group respondents according to their experiences of technology use and a path analysis to estimate the effects of care workers’ characteristics and prior experiences on the perceived benefits of videoconferencing tools in eldercare work. Results We found that the personal characteristics of a care worker are associated with both the use of videoconferencing and its perceived benefits and drawbacks. In addition, we discovered that prior use of videoconferencing tools, especially in direct care work, affects the perceived benefits of them. Those who have used videoconferencing in direct care work perceive them as more beneficial for both their own work and for clients. Conclusions Based on these results, we suggest that healthcare and social welfare organizations pay attention to the characteristics of care workers, especially when estimating the necessary amount of ICT support. More ICT support should be targeted to those with a lower level of education and higher age.

Background The prevalence of bronchiectasis is significantly higher among adult Aboriginal Australians (the Indigenous peoples of Australia) compared to non-Aboriginal Australians. Currently, there is no well-established tool to assess bronchiectasis severity specific to Indigenous peoples. Nor has the applicability and validity of the two well-established bronchiectasis severity assessment tools - The “Bronchiectasis Severity Index” (BSI) and “FACED” scale been vigorously tested in an Indigenous population. This retrospective study evaluated the validity of the BSI and FACED amongst an adult Aboriginal Australian cohort with bronchiectasis in the Top End Northern Territory (NT) of Australia. Methods Patients with CT confirmed bronchiectasis identified between 2011 and 2020, residing in the Top End of the NT were eligible to be enrolled. The primary endpoint of 4-year mortality was assessed via hospital records, and sensitivity and specificity of the BSI and FACED assessed against this using area under the curve (AUC) receiver operating characteristics analysis. For patients with missing data, a relative BSI / FACED score was used which divided the score recorded for that patient by the total potential score based on their available clinical data. Results A total of 456 adult Aboriginal Australian patients >18 years of age were included (55.5% female, median age 49 years). According to the BSI score 43.4% of patients were assessed to have mild, 30.5% moderate and 26.1% severe bronchiectasis (median score 4 (IQR 2, 8)). According to the FACED 80.9% were assessed to have mild, 17.8% moderate and 1.3% severe (median score of 1 (IQR 0, 2)). Four-year mortality was 11.2% (median age of death 55.6 years). Sensitivity and specificity of the BSI combining moderate and severe were 86.3 and 47.2% respectively, and for severe alone 51% and 77%. Sensitivity and specificity of the FACED combining moderate and severe were 21.6% and 81.2%, respectively, and for severe alone 2% and 98.8%. The AUC for the continuous total BSI was 0.703, and the FACED 0.515. Utilising a relative score, based only on data available for patients with missing data (ie lung function or BMI) resulted in slightly improved AUCs for both the BSI (0.717) and FACED (0.571). Conclusion Both BSI and FACED bronchiectasis assessment tools may not be ideal in an Indigenous/Aboriginal people’s context. However, it may be reasonable to utilise the relative BSI score in this population until Indigenous people’s specific bronchiectasis severity assessment tools are developed.

Drawing on ethnographic fieldwork in Finland, we report on the trial of a teleoperated care robot named Välkky introduced onto a fully operational hospital neurological ward. Our data revealed a narrative arc where participants’ early expectations of the hospital-based trial altered as the project unfolded. Greeted with techno-excitement and experimental enthusiasm about the place of robotics in reshaping roles within clinical care, Välkky became the focus for collaborative in situ learning, adaptation and redesign amongst the roboticists, designers, nurses, patients, and managers. Välkky acted as an ‘attractor’ provoking thinking about, and a reimagining of, future arrangements of care. Our empirically informed insights seek to pave the way for real-world nuanced thinking that pushes beyond human/non-human and success/failure binaries. Building on debates in STS and feminist posthumanism, we propose a robocentric approach, which encourages us to ‘queer’ health care robots, and to understand them as fluid, hybrid, distributed and relational figures, rather than purely as inert, mechanical, non-human objects that might replace humans. Nursing care practices by and with robots will generate new meanings and practices of care that will emerge iteratively, as caring relations, relationships and practices develop within the context of operational ward environments. Robots may or may not be able support care, but they will invariably challenge what care is.

Due to methodological reasons, the X-chromosome has not been featured in the major genome-wide association studies on Alzheimer’s Disease (AD). To address this and better characterize the genetic landscape of AD, we performed an in-depth X-Chromosome-Wide Association Study (XWAS) in 115,841 AD cases or AD proxy cases, including 52,214 clinically-diagnosed AD cases, and 613,671 controls. We considered three approaches to account for the different X-chromosome inactivation (XCI) states in females, i.e. random XCI, skewed XCI, and escape XCI. We did not detect any genome-wide significant signals (P ≤ 5 × 10⁻⁸) but identified seven X-chromosome-wide significant loci (P ≤ 1.6 × 10⁻⁶). The index variants were common for the Xp22.32, FRMPD4, DMD and Xq25 loci, and rare for the WNK3, PJA1, and DACH2 loci. Overall, this well-powered XWAS found no genetic risk factors for AD on the non-pseudoautosomal region of the X-chromosome, but it identified suggestive signals warranting further investigations.

Achieving resolution in the sub-Rayleigh regime (superresolution) is one of the rapidly developing topics in quantum optics and metrology. Recently, it was shown that perfect measurement based on spatial mode demultiplexing (SPADE) in Hermite–Gauss modes allows one to reach the quantum limit of precision for estimation of separation between two weak incoherent stationary sources. Since then, different imperfections such as misalignment or crosstalk between modes have been studied to check how this result translates into more realistic experimental setups. In this paper, we consider another deviation from the perfect setup by discarding the assumption about the stationarity of the sources. This is relevant for example for astrophysical applications where planets necessarily orbit around the star. We analyze two examples of dynamics: rotations and oscillations, showing the robustness of the SPADE-based measurement against them. The analysis is based on Fisher information, which allows one to obtain the precision limit through Cramér–Rao bound. Furthermore, we formulate a measurement algorithm that allows for the reduction of one parameter for estimation (system orientation angle) in the stationary sources scenario, maintaining the measurement precision despite the lack of knowledge about this parameter.

Angiotensensin-converting enzyme-2 (ACE2) is a receptor for SARS-CoV-2, allowing the virus to enter cells. Although tumor patients infected by SARS-CoV-2 often have a worse outcome, the expression, function and clinical relevance of ACE2 in tumors has not yet been thoroughly analyzed. In this study, RNA sequencing (RNA-seq) data from tumors, adjacent tissues and whole blood samples of COVID-19 patients from genome databases and from tumor cell lines and endothelial cells infected with different SARS-CoV-2 variants or transfected with an ACE2 expression vector (ACE2high) or mock (ACE2low) were analyzed for the expression of ACE2 and immune response relevant molecules in silico or by qPCR, flow cytometry, Western blot and/or RNA-seq. The differential expression profiles in ACE2high vs. ACE2low cells correlated with available SARS-CoV-2 RNA-seq datasets. ACE2high cells demonstrated upregulated mRNA and/or protein levels of HLA class I, programmed death ligand 1 (PD-L1), components of the antigen processing machinery (APM) and the interferon (IFN) signaling pathway compared to ACE2low cells. Co-cultures of ACE2high cells with peripheral blood mononuclear cells increased immune cell migration and infiltration towards ACE2high cells, apoptosis of ACE2high cells, release of innate immunity-related cytokines and altered NK cell-mediated cytotoxicity. Thus, ACE2 expression was associated in different model systems and upon SARS-CoV-2 infection with an altered host immunogenicity, which might influence the efficacy of immune checkpoint inhibitors. These results provide novel insights into the (patho)physiological role of ACE2 on immune response-relevant mechanisms and suggest an alternative strategy to reduce COVID-19 severity in infected tumor patients targeting the ACE2-induced IFN-PD-L1 axis. Supplementary Information The online version contains supplementary material available at 10.1007/s00018-024-05520-9.

This preliminary study investigated if VEGFR-2 selective adenoviral Vammin (AdVammin) gene therapy could induce angiogenesis and increase perfusion in the healthy porcine myocardium. Also, we determined using a clinically relevant large animal model if AdVammin gene therapy could improve the function of a chronically ischemic heart. Low doses of AdVammin (dose range 2 × 10⁹–2 × 10¹⁰ vp) gene transfers were performed into the porcine myocardium using an endovascular injection catheter. AdCMV was used as a control. The porcine model of chronic myocardial ischemia was used in the ischemic studies. The AdVammin enlarged the mean capillary area and stimulated pericyte coverage in the target area 6 days after the gene transfers. Using positron emission tomography ¹⁵O-radiowater imaging, we demonstrated that AdVammin gene therapy increased perfusion in healthy myocardium at rest. AdVammin treatment also increased ejection fraction at stress in the ischemic heart, as detected using left ventricular cine angiography. In addition, we demonstrated successful in vivo imaging of enhanced angiogenesis using [⁶⁸Ga]NODAGA-RGD peptide. However, AdVammin also increased tissue permeability and was associated with significant pericardial fluid accumulation, limiting AdVammin’s therapeutic potential and emphasizing the importance of correct dosage.