Universidade do Vale do Taquari - UNIVATES
  • Lajeado, Estado do Rio Grande do Sul, Brazil
Recent publications
The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. K E Y W O R D S follicle, ovary, ovine, proteins, reproduction Mol Reprod Dev. 2022;1-12. wileyonlinelibrary.com/journal/mrd
Este estudo tem como objetivo abordar o modo como os Kaingang da Terra Indígena Foxá, no município de Lajeado (RS), percebem as políticas públicas brasileiras de saúde indígena e a relação saúde-doença, durante o período pandêmico de Covid-19. A metodologia utilizada elenca a abordagem etno-histórica e o caráter interdisciplinar no tratamento dos dados sobre o povo indígena Kaingang. Tratou-se de uma pesquisa qualitativa, de natureza descritiva, utilizando a revisão bibliográfica construída a partir de fontes documentais e orais sobre saúde indígena, com o uso de diários de campo e entrevista in loco, com as lideranças indígenas Kaingang, da Terra indígena Foxá. Os principais resultados evidenciaram que apesar da legislação brasileira garantir aos povos indígenas o atendimento diferenciado, com respeito à diversidade cultural-religiosa, o acolhimento às demandas dos indígenas Kaingang investigados é pouco efetivo, uma vez que os profissionais de saúde tendem a não compreender a perspectiva indígena na relação saúde-doença, sendo esta mais complexa em tempos de pandemia. Constatou-se que, para os Kaingang da Terra Indígena Foxá, a relação saúde-doença está ligada ao rompimento (parcial) do homem com a natureza, incluindo seus ciclos. À vista disso, as políticas públicas de saúde não contemplam adequadamente os aspectos da natureza da mesma forma que na concepção dos indígenas, distanciando ambas as culturas.
Non-conventional food plants have a variety of bioactive compounds with nutritional value. Vasconcellea quercifolia A. St.-Hil., belonging to the Caricaceae family, is a dietary alternative with excellent nutritional composition. This study aimed at characterizing the nutritional composition of mountain papaya (V. quercifolia) green fruit flour, in order to incorporate it in a functional food. For that purpose, the flour was characterized regarding its macro and micronutrients, anti-nutritional factors, pH, water activity, and color. This flour showed contents of carbohydrate of 22.31%; protein of 9.65%; dietary fiber of 32.80%; lipids of 14.95%, 63.56% of which are unsaturated fatty acids, especially oleic acid; and ash of 9.10%, with higher concentrations for potassium, calcium and magnesium. Therefore, V. quercifolia flour had good nutritional characteristics and might be used as supplementary food.
The present study involves children diagnosed with Attention Deficit Hyperactivity Disorder (ADHD) from public schools of the municipality of Crato - CE, northeastern region of Brazil. The overall objective was to analyze the profiles of six children, before and after interventions with nature, in order to identify potential changes in behavior resulting from experiencing direct contact with nature. The method used is exploratory and descriptive, and it is a multiple case study with an action-research character. The results showed changes in behavior with mitigated ADHD symptoms regarding cognitive and socio-affective aspects. Among other behavioral aspects, higher motivation for studying, better understanding and adaptation to rules, extended states of tranquility, higher receptivity to social contact, decreased aggressiveness, hyperactivity, impulsivity, and higher tolerance to oneself and others were noticeable.
RESUMO Objetivo Investigar a saúde mental de pais com filhos pequenos durante a pandemia de COVID-19. Métodos Foram utilizados questionários elaborados pelo Google Forms, no qual foram aplicados os dados sociodemográficos, e as escalas EADS-21, IES-R, PSQI-BR e WHOQOL-BREF. As análises estatísticas foram realizadas no software estatístico SPSS 21.0 para Windows. Para descrição da amostra, foram utilizadas análises descritivas. Em todos os testes, foi considerada a significância estatística de 0,05. Os 327 participantes foram divididos em três grupos: voluntários sem filhos (Grupo 1), com filhos entre 0 e 6 anos (Grupo 2) e com filhos de 7 anos ou mais (Grupo 3), dos quais todos eram residentes da região Sul do Brasil. Resultados Os grupos com maior vulnerabilidade em relação à sua saúde mental é o de participantes sem filhos e com filhos de 0 a 6 anos, com maiores escores para as escalas de depressão, ansiedade e estresse, e estresse pós-traumático, em comparação com o grupo com filhos de 7 anos ou mais. Conclusões Mesmo que os dados mostrem que todos os grupos estão dentro da normalidade para as escalas aplicadas, faz-se necessário atentar para a saúde mental da população em maior sofrimento psíquico, seja a partir de estratégias desenvolvidas por profissionais de rede de saúde (SUS) e/ou da assistência social (SUAS), seja por projetos municipais/estaduais na busca pela promoção da saúde mental na rede.
Farming expansion has negative impacts on freshwater biodiversity. However, the effects of agricultural land use are not similar across taxa and depend on local context. For instance, the impacts of agricultural expansion are understudied in the Neotropics (one of the leading regions in cropland expansion). Knowledge of the effects of agricultural land use on aquatic insects from South American subtropical grasslands (Pampa) is even more incipient. We tested whether landscape modification related to increased agricultural land use was associated with taxonomic homogenization in odonate communities in waterbodies in the Brazilian Pampa. Odonates were collected in waterbodies differing in the main land-use class in their surroundings (cropland or grassland). Cropland and grassland sites differed with respect to their abiotic conditions (water chemistry) and species composition of Odonata. Additionally, we found higher variation in the composition of Odonata (and suborders Anisoptera and Zygoptera separately) in grassland than cropland sites. We found an interplay between agricultural and grassland land uses and the variation in the composition of odonate communities in the Brazilian Pampa. Specifically, landscape modification by agriculture modified the abiotic conditions in the waterbodies, which may have favored species able to establish as larvae under harsher environmental conditions. Implications for insect conservation We suggest that the maintenance of mixed-grassland and cropland land uses in the fields adjacent to waterbodies can limit the negative effects of agricultural encroachment on Odonata communities with respect to biotic homogenization in the Brazilian Pampa.
The inflammatory process is a biological response of the organism to remove injurious stimuli and initiate homeostasis. It has been recognized as a key player in the most severe forms of SARS-CoV-2, characterized by significantly increased pro-inflammatory cytokine levels, the so-called "cytokine storm" that appears to play a pivotal role in this disease. Therefore, the aim of this systematic review was to select clinical trials with anti-inflammatory plants and relate the activity of these plants to inflammatory markers of SARS-CoV-2 infection. PRISMA guidelines are followed, and studies of interest are indexed in PubMed and ClinicalTrials.gov databases. As a result, 32 clinical trials encompassing 22 plants were selected. The main anti-inflammatory mechanisms described in the studies are the inhibition of inflammatory cytokines, such as IL-6, TNF-a, IFN-γ, and IL-1; decreased CRP and oxidative marker levels; increased endogenous antioxidant levels; modulation of cardiovascular risk markers. The data found are not directly related to SARS-CoV-2 infection. However, they provide possibilities for new studies as plants have a wide array of phytochemicals, and detecting which ones are responsible for anti-inflammatory effects can provide invaluable contribution to studies aiming to evaluate efficacy in scenarios of SARS-CoV-2 infection.
Flexibility and function are related properties in the study of protein dynamics. Flexibility reflects in the conformational potential of proteins and thus in their functionalities. The presence of interactions between protein-ligands and protein-protein complexes, substrates, and environmental changes can alter protein plasticity, acting from the rearrangement of the side chains of amino acids to the folding/unfolding of large structural motifs. To evaluate the effects of the flexibility in protein systems, we defined the enzyme 2-trans-enoyl-ACP (CoA) reductase from Mycobacterium tuberculosis, or MtInhA, as our target system. MtInhA is biologically active as a tetramer in solution; however, computational studies commonly use the monomer justifying the independence of its active sites due to their distances. However, differences in flexibility between tertiary and quaternary structures could present impact on the size of the active site, influencing the drug discovery process. In this study, we investigated the influence of flexibility restrictions in A- and B-loops of the MtInhA in order to suggest a monomeric structure that describes the conformational behavior of the tetrameric system. Overall, we observed that simulations where restrictions were applied to the A- and B-loops present a more similar behavior to the native structure when compared to unrestricted simulations. Therefore, our work presents a monomeric model of MtInhA, which has conformational characteristics of the biologically active structure. Thus, the data obtained in this work can be applied to the MtInhA system for the generation of more reliable flexible models for molecular docking experiments, and also for the performance of longer simulations by molecular dynamics and with a lower computational cost.
Gastrointestinal tract diseases are characterized by an imbalance in physiological functions. These may involve inflammatory and metabolic pathways, triggering chronic, multifactorial and idiopathic inflammatory disorders. The association of probiotics with prebiotics has the potential to remedy these afflicting conditions, since they attenuate or even block the adhesion of pathogenic microorganisms in the enteric environment. This article highlights the importance of using probiotics associated with fibers from Psyllium as prebiotics, to maintain a healthy intestinal microbiota. We also present the technologies and encapsulating materials involved in coating to increase the survival rate of these strains when exposed to the gastrointestinal tract. The importance of products containing probiotics and fibers from Psyllium as prebiotics become increasingly evident when there is a health bias. Emerging health challenges and advances in research will drive selective approaches in biotechnology to discover and evaluate new probiotics and prebiotics that can potentially contribute to human health.
This work aimed at studying a composite material for shoe assembly insole using polyester fabric waste (PFW) from the footwear industry. For the preparation of the composites, polyvinyl acetate (PVAc) was employed as the polymeric matrix, with 5%, 10%, 15%, and 20% in weight of PFW in the formulations and compared with a standard formulation without PFW. The samples were characterized via physical–chemical (moisture content, bulk density, and growth/shrinkage) and mechanical (break, tensile and dry, and wet tear) analyses, and also by scanning electron microscopy. The PVAc/PFW 5% and 10% composites showed satisfactory results in all tests performed. They reached 9.99 and 9.09 MPa tensile strength, respectively, twice the minimum acceptable (5 MPa), and 1.13 and 1.07 N/mm dry tear strength, also higher than required (1.0 N/mm). The better results for the samples with less PFW were justified by the lower tendency to produce agglomerates during the molding and compaction production stages. Compared with commercial materials commonly used in shoes, these samples met all specifications and showed the technical feasibility of reusing PFW in assembly insoles. Polyester fabric waste (PFW) and polyvinyl acetate (PVAc) were used to produce a composite for a shoe assembly insole.
Nature has revealed to be a key source of innovative anticancer drugs. This study evaluated the antitumour potential of the marine bromoditerpene sphaerococcenol A on different cancer cellular models. Dose-response analyses (0.1–100 µM; 24 h) were accomplished in eight different tumour cell lines (A549, CACO-2, HCT-15, MCF-7, NCI-H226, PC-3, SH-SY5Y, SK-MEL-28). Deeper studies were conducted on MFC-7 cells, namely, determination of hydrogen peroxide (H2O2) levels and evaluation of apoptosis biomarkers (phosphatidylserine membrane translocation, mitochondrial dysfunction, Caspase-9 activity, and DNA changes). The ability of the compound to induce genotoxicity was verified in L929 fibroblasts. Sphaerococcenol A capacity to impact colorectal-cancer stem cells (CSCs) tumourspheres (HT29, HCT116, SW620) was evaluated by determining tumourspheres viability, number, and area, as well as the proteasome inhibitory activity. Sphaerococcenol A hepatoxicity was studied in AML12 hepatocytes. The compound exhibited cytotoxicity in all malignant cell lines (IC50 ranging from 4.5 to 16.6 µM). MCF-7 cells viability loss was accompanied by H2O2 generation, mitochondrial dysfunction, Caspase-9 activation and DNA nuclear morphology changes. Furthermore, the compound displayed the lowest IC50 on HT29-derived tumourspheres (0.70 µM), followed by HCT116 (1.77 µM) and SW620 (2.74 µM), impacting the HT29 tumoursphere formation by reducing their number and area. Finally, the compound displayed low cytotoxicity on AML12 hepatocytes without genotoxicity. Overall, sphaerococcenol A exhibits broad cytotoxic effects on different tumour cells, increasing H2O2 production and apoptosis. It also affects colorectal CSCs-enriched tumoursphere development. These data highlight the relevance to include sphaerococcenol A in further pharmacological studies aiming cancer treatments.
ABSTRACT As an indicator of surface moisture, TVDI has great potential for application in agriculture in different types of climates, but the best results have been obtained in arid climates, given the presence of areas with contrasting water conditions. Some studies under subtropical climate conditions have shown good results in the use of TVDI as an indicator of surface moisture but some uncertainties still need to be better understood. This study aimed to evaluate the coherence of temperature-vegetation dryness index (TVDI) values, obtained from sensors installed on the surface, compared to data obtained using orbital images in an agricultural area in southern Brazil under humid subtropical climate conditions. The analyses employed normalized difference vegetation index (NDVI) and surface temperature (TS) data obtained through sensors installed on the surface, with continuous monitoring, as well as Landsat OLI/TIRS images, covering the period of the soybean cycle. The TVDI values, obtained from NDVI and TS radiometric sensors installed in the field, indicated consistency of using Landsat-OLI/TIRS orbital images to map the surface moisture condition, representing the spatial and temporal variations of the water condition of soybean cultivations under subtropical climate conditions.
Copper (Cu) is essential to plants but can also be harmful due to Fenton chemistry. Because of that, it is necessary to keep Cu within a narrow concentration limit. Plants evolved mechanisms to sense Cu and precisely regulate uptake and accumulation to avoid both deficiency and toxicity. However, plants experience variable Cu levels in soils, both due to natural environments as well as human’s-based farming practices that can lead to Cu accumulation in soils. Therefore, we need to understand Cu homeostasis. Here, we review how Cu is found in soils; Cu toxicity responses in plants; the role of Cu transporters, described mainly in model species such as Arabidopsis thaliana and Oryza sativa; the crosstalk between Cu and iron (Fe) homeostasis; Cu hyperaccumulator plants; and discuss some gaps and future directions, which can improve our understanding in the field.
Tobacco is a rich source of cellulosic material and one of the most cultivated non-food plants in the world with great potential for incorporation in polymeric matrices. The use of tobacco residues as reinforcing filler requires chemical/physical fiber treatment aiming to maximize compatibility with the polymer. In this study, tobacco residues were treated with two concentrations of NaOH (10 or 15 wt.%) at two-time exposures (3 or 5 h). Four distinct heating rates were used for each condition. It was applied an artificial neural network to model the thermogravimetric curves. After, the fitted ANN curves were used to create a 3D surface response. The equations from 3D surface response allowed the creation of thermogravimetric curves in any heating rate situated between the minimum and maximum range tested.
Vineyards are a favorable culture for the habitat of phytophagous mites. These pests feed on the cellular sap of vegetables, causing damage to plants. The aim of this study was to comparatively evaluate the effects of applied biological control of Panonychus ulmi (Koch) (Tetranychidae) using Neoseiulus californicus (McGregor) (Phytoseiidae) on the physicochemical properties and bioactive compounds profile in grapes of Merlot variety, in one single property in the municipality of Bento Gonçalves, Rio Grande do Sul, Brazil. Treatments were conducted in three different areas of the vineyard: area attacked by P. ulmi (AP), area with P. ulmi controlled by N. californicus (APN), and a third area without the presence of mites (AWM). Berries in the AP treatment had lower hardness, with both test probes used (cutting line type and cylinder type). Grapes of AWM treatment had higher red coloration in parameter ‘a*’, both in the skin and in the pulp. Sugar content did not significantly differ between fruits from grapevines with presence of mites. In grape skins of AP treatment, total phenol, flavonoid, and anthocyanin contents were significantly higher, whereas resveratrol content was lower. Compared to fruits of grapevines without mites (AWM treatment), grapes with biological treatment (APN) had significantly higher contents of sugar, total phenols (pulp and skin), flavonoids (skin), anthocyanins (skin), and antioxidants (skin and pulp). Results suggest that biological control applied using predatory mites increases the concentration of phenolic compounds in fruits, improving the nutritional quality of the grapes.
Myrciaria plinioides D. Legrand (Myrtaceae) is a native plant of Southern Brazil, which have potential in the food industry due to its edible fruits. Many plants belonging to this genus have been used for a variety of illnesses, including inflammatory disorders due to antioxidant properties. However, therapeutic uses of M. plinioides have been poorly studied. The aim of study was to assess the anti-inflammatory and anticoagulant activities of the ethanol leaf extract of M. plinioides . In M. plinioides extract-treated RAW 264.7 cells, assessments of cell viability, TNF-α release and p38 MAPK pathway-dependent protein expression were detected. In addition, rat paw edema models were used to analyze the anti-inflammatory effect of the extract. Macrophages cell line treated with M. plinioides extract showed a slight decrease in cell viability. In LPS-stimulated macrophages treated with different concentrations of the extract for 24 h, TNF-α release was inhibited, while modulation of p38 signaling pathway and inhibition of NF-κB p65 protein expression were dose-dependent. In rats, the extract inhibited the formation of paw edema, while an inhibitory effect on trypsin-like enzymes derived from mast cells was seen. Furthermore, the extract presented anticoagulant activity via extrinsic pathway, being able to block specifically factor Xa and thrombin. The study suggests that extract possess potent anti-inflammatory and anticoagulant effects. M. plinioides present great biological potential as a source for the development of anti-inflammatory and anticoagulant drugs. Additional studies can be proposed to better elucidate the mechanism by which M. plinioides exerts its effects.
The research aimed to analyze men's participation in groups of pregnant women for the manifestation of the bond and the role with their child during the pregnancy-puerperal period. Data survey took place from a focus group of three virtual meetings, held every two weeks with pregnant couples. Through the Discursive Textual Analysis, it can be seen that the group of pregnant women helped in the improvement of motherhood and fatherhood and in strengthening the bond that already existed in the couple, reflecting in the care and participation throughout the entire gestational process, as well as in the baby's first years.
Este artigo problematiza a conexão da Biologia e da Matemática, considerando a prática pedagógica, por meio de uma revisão bibliográfica a partir da busca de produções que descrevem situações da relação da Biologia e da Matemática, considerando práticas efetivas pautadas nessa relação, utilizadas para potencializar principalmente o ensino de conteúdos de Genética na educação básica. A coleta de dados foi realizada utilizando-se para a pesquisa as bases de dados: Biblioteca digital USP – Teses e Dissertações, Acervo UNESP e no Portal de Periódicos Capes/MEC, dentro de um recorte temporal entre os anos de 2010 a 2020. Os resultados apontam existir escassez de pesquisas com foco na relação destas duas unidades curriculares associadas a práticas de ensino e aprendizagem. Observa-se a importância da conexão entre a Biologia e Matemática em situações de ensino e de aprendizagem e a relevância de se buscar que o aluno seja autônomo, reflexivo no seu processo de aprendizagem.
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961 members
Marcus Cristian Muniz Conde
  • Centro de Ciências Biológicas e da Saúde - Curso de Odontologia
Andre Jasper
  • Life Sciences
Luis Fernando Saraiva Macedo Timmers
  • Department of Biotechnology
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Address
Avenida Avelino Tallini, 95.914-014, Lajeado, Estado do Rio Grande do Sul, Brazil
Head of institution
Ney José Lazzari
Website
http://www.univates.br/
Phone
+55(51)3714-7000
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+55(51)3714-7001