• João Pessoa, Paraiba, Brazil
Recent publications
The Atlantic and Amazon rainforests have a shared but unclear past, with intermittent connections resulting from historical climate change. We investigate these connections by studying the phylogeography and climatic niche of the disjunct distributed frog Lithobates palmipes. We sequenced two fragments of mitochondrial DNA from Atlantic Forest (AtF) and Amazonia (AmF) individuals and evaluated how genetic diversity is distributed in space and whether past demographic changes occurred. Also, we evaluated the existence of past suitable connections between biomes for L. palmipes through ecological niche models (ENM) and tested for niche divergence. The AtF group is nested within the AmF group and closely related to individuals from eastern Amazonia, a pattern recovered in many species that used northeast connection routes. We found evidence of recurrent use of connections in different directions and time during the Pleistocene, resulting in genetic structure between biomes, with no signal of demographic change and evidence of niche divergence across both genetic groups. ENMs indicated suitable areas connecting forests throughout northeastern Brazil during the Pleistocene. Mitochondrial lineages do not match biomes exactly. One lineage is composed of AtF populations and eastern Amazonia individuals. The other is composed of western Amazonia individuals, suggesting an effect of past climatic heterogeneity within the Amazonia forest. This is the first evidence that this route drove genetic and ecological diversity for amphibians recently, a group with habits and ecological requirements different from other vertebrates that have been shown to use this putative corridor.
Objective To provide guidelines on the coronavirus disease 2019 (COVID-19) vaccination in patients with immune-mediated rheumatic diseases (IMRD) to rheumatologists considering specific scenarios of the daily practice based on the shared-making decision (SMD) process. Methods A task force was constituted by 24 rheumatologists (panel members), with clinical and research expertise in immunizations and infectious diseases in immunocompromised patients, endorsed by the Brazilian Society of Rheumatology (BSR), to develop guidelines for COVID-19 vaccination in patients with IMRD. A consensus was built through the Delphi method and involved four rounds of anonymous voting, where five options were used to determine the level of agreement (LOA), based on the Likert Scale: (1) strongly disagree; (2) disagree, (3) neither agree nor disagree (neutral); (4) agree; and (5) strongly agree. Nineteen questions were addressed and discussed via teleconference to formulate the answers. In order to identify the relevant data on COVID-19 vaccines, a search with standardized descriptors and synonyms was performed on September 10th, 2021, of the MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, ClinicalTrials.gov, and LILACS to identify studies of interest. We used the Newcastle–Ottawa Scale to assess the quality of nonrandomized studies. Results All the nineteen questions-answers (Q&A) were approved by the BSR Task Force with more than 80% of panelists voting options 4—agree—and 5—strongly agree—, and a consensus was reached. These Guidelines were focused in SMD on the most appropriate timing for IMRD patients to get vaccinated to reach the adequate covid-19 vaccination response. Conclusion These guidelines were developed by a BSR Task Force with a high LOA among panelists, based on the literature review of published studies and expert opinion for COVID-19 vaccination in IMRD patients. Noteworthy, in the pandemic period, up to the time of the review and the consensus process for this document, high-quality evidence was scarce. Thus, it is not a substitute for clinical judgment.
Sjogren's Syndrome (SS) is an autoimmune disease characterized by lymphocytic infiltration of the exocrine glands and other organs, associated with sicca syndrome but also with systemic involvement with varying degrees of severity. Despite their importance, these systemic manifestations are not routinely evaluated and there is no homogenous approach to their diagnosis or evaluation. To close this gap, a panel of experts from the Brazilian Society of Rheumatology conducted a systematic review and meta-analysis on the identification of epidemiologic and clinical features of these manifestations and made recommendations based on the findings. Agreement between the experts was achieved using the Delphi method. The first part of this guideline summarizes the most important topics, and 11 recommendations are provided for the articular, pulmonary, and renal care of SS patients.
Background We examined the influence of superoxide dismutase 3 (SOD3) Arg213Gly and Peroxisome Proliferator-Activated α-Receptor (PPARα) 7G/C polymorphisms to a single dose of purple grape juice supplementation on time-to-exhaustion running test, redox balance and muscle damage in recreational runners. Methods Forty-seven male recreational runners performed a running test until exhaustion after supplementation with grape juice or a control drink. Serum total antioxidant capacity (TAC), malondialdehyde (MDA), plasma nitrite (NO), creatine kinase (CK) and lactate dehydrogenase (LDH) were measured pre and post exercise. Also, polymorphisms were analyzed in DNA extracted from the oral mucosa. Results Grape juice improved the time-to-exhaustion. When analyzed by genotype, the recreational runners with GG+CG genotypes of the SOD3 gene had greater time-to-exhaustion than the CC genotype, but was no different for the PAPRα gene. A slight difference was noted in TAC, since the CC genotype of the SOD3 gene showed higher TAC values in the post-exercise compared to the baseline and with pre-exercise, but these values did not increase compared to the CG+GG group, respectively. The SOD3 and PPARα genes were similar at all times for the other biochemical variables. Conclusion The ergogenic effect of grape juice was genotype-dependent for SOD3 Arg213Gly. However, biochemical redox balance markers did not explain this difference.
The Valle de Tenza region, located in the Department of Boyacá—Colombia, shows a transition situation from the family farming of various food crops to coffee farming following an agribusiness model. From this perspective, in order to understand the current scenario of food sovereignty in Guateque and Guayatá, two cities of the Valle de Tenza, this study evaluated socioeconomic, environmental, and cultural aspects based on questionnaires and semi-structured interviews applied to peasant families that practice family farming and/or coffee farming. Moreover, these same aspects were also evaluated among urban food consumers. These evaluations aimed to assess the perception of the interviewees about the availability of regional food crops and current eating habits in relation to those from a decade or more ago, in addition to investigating their knowledge about the traditional cuisine of the region. The cultivation of regional food crops used to prepare local and regional traditional dishes such as piquete , sancocho , and different amasijos based on corn and sagú ( Maranta arundinacea ) has been significantly reduced. The investigation revealed changes in the eating habits of the Valle de Tenza inhabitants due to the consumption of processed foods and the reduced cultivation of local traditional food crops. As a consequence of this transition to coffee production, the most representative traditional foods are being replaced by more profitable crops, including coffee and some fruits not traditionally grown in the Valle de Tenza and with more local and regional acceptance. This reduction can affect food availability and change the gastronomic and cultural identity of the Valle de Tenza population, among other aspects related to food sovereignty.
Candida species are considered opportunistic microorganisms present in the normal microbiota of the oral cavity and gastrointestinal and urogenital tracts of human beings. These infections are more common in individuals with compromised immune systems and their frequency has increased over the years. Regarding bioproducts, recent research from natural sources suggest that algae are a promising group in providing new bioactive substances. The objective of this work is to evaluate the antifungal activity and the in vitro modifying potential of the dichloromethane/methanol extract of G. cervicornis against Can-dida spp. Tests were performed using broth microdilution method to determine the intrinsic activity of the products, and combination therapy with commercial drugs. The intrinsic activity of DMEGC showed fungistatic activity against all Candida species tested at the concentration of 1024 μg/mL. DMEGC at subin-hibitory concentrations was able to modify the action of Fluconazole against isolated and standard C. kru-sei , reducing the amount of drug needed to inhibit the growth of the microorganism by 50% (IC50) by more than 12-fold. The results show that DMEGC can be used to treat C. krusei in combination therapy with commercial antifungals such as Fluconazole. In addition, its fungistatic activity is important to reduce the development of drug resistance. Further studies are needed to verify which compounds isolated from the extract were responsible for its antifungal activity and which mechanisms of action.
Eco-innovation is the innovation of products that focuses on the use of renewable energy and meets specific environmental requirements, and is essential for energy conservation and environmental protection. There are recommendations and strategies available for general product development, but there are limited studies when focusing on renewable energy products. A proposal for eco-innovation in renewable energy is developed based on exergy analysis, TRIZ methodology, and knowledge management. The model is demonstrated with a specific case study. This study contributes to the analysis and development of products with renewable energy that help mitigate climate change, consistent with the change in the energy matrix. Furthermore, this study is a transdisciplinary effort that integrates concepts from different topics to achieve a comprehensive model.
Urban forests are responsible for a lot of benefits, and can be used as a tool to ex-situ conservation of native species. Our study investigated a public urban forest aiming to provide subside to management projects in order to allow the accurate fulfillment of social, aesthetic and ecological functions displayed by trees. Our survey was conducted in 6 public squares and 5 gardens in the Jardins neighborhood. We classified the trees according to their phytogeographic origins and seed dispersal habit. The Shannon-Weaver (H') index, Simpson's dominance (D') and Pielou's equability (J) were determined. A total of 507 individuals were sampled, located in 12 botanical families and 29 species, and 13 of them were classified as native and 16 as exotic. The family with higher species richness and number of species was Fabaceae (36%). Regarding the seed dispersal syndrome, the most representative was zoochory (52%). The ecological index showed medium species diversity (H'=2.2284), high dominance (D'=0.7899) and medium number of individuals (J) 0.6552. Although the Jardins neighborhood was planned, the urban forest in this area still needs improvement; such as incentive measures that can promote biodiversity and the application of ex-situ conservation.
Drought stress is one of the main causes of low crop productivity due to disturbances in plant growth, physiology and biochemical processes. Spermine application is an alternative to mitigate the harmful effects of this stress on plants. Nasturtium (Tropaeolum majus L.) is an ornamental, medicinal and edible plant cultivated and consumed in several countries. This plant has medicinal properties (among them the presence of glucosinolates and phenolic compounds), in addition to being appreciated in cooking. Thus, the objective of this work was to evaluate the effects of spermine application on growth, gas exchange and antioxidant metabolism of T. majus grown under drought stress. Growth (stem diameter, number of leaves, number of flowers, number of buds, total dry mass and leaf area), gas exchange (gs, A, E and Ci), relative water content, free amino acids, phenolic compounds, reducing and non-reducing sugars, lipid peroxidation and enzymatic activity (CAT, POD and APX) were evaluated. Spermine reduced the harmful effects of severe drought stress on the relative water content, reducing sugars and non-reducing sugars. Spermine reduced the harmful effects of moderate drought stress on the relative water content, amino acids, reducing sugars and non-reducing sugars. Spermine application decreased lipid peroxidation (MDA) of T. majus under severe and moderate drought stress and in plants without stress. Spermine increased the activity of POD and APX in plants under severe drought stress and of CAT, POD and APX in plants under moderate drought stress. Spermine application increases the drought tolerance of T. majus by increasing morphology, gas exchange, relative water content, amino acids, reducing and non-reducing sugars, CAT, POD and APX enzymes, and decreasing lipid peroxidation. Spermine (1 mM) can be used to mitigate the harmful effects of drought stress in T. majus.
This paper presents static analysis of a double-plate system using the boundary element method. The system is composed of two parallel plates with an elastic layer in between. The plates are assumed to be represented by the Kirchhoff plate model and the elastic layer is treated as a Winkler elastic foundation. The mathematical steps required to establish a boundary element technique are adequately addressed: integral equations, fundamental solution; domain integral transformation into boundary integral, and formation of an algebraic system of the double-plate problem. Numerical examples of double plate problems with different mechanical properties, boundary conditions, and load types are presented.
Coordination polymers (CPs) have been on focus as a breakthrough material to catalyze organic reactions, especially CC bond formations. Recently, few reports showed potentialities of CPs as new catalysts for Morita-Baylis-Hillman (MBH) reactions, but still with unsatisfactory yields and reaction times. Thus, this work explores the synthesis and characterization of a mixed-metal copper-manganese iminodiacetate 1D coordination polymer, named here Cu/Mn-IDA and its application as catalyst for MBH reaction. The catalyst was obtained at room temperature synthesis, characterized by infrared absorption spectroscopy (FT-IR), X-ray powder diffraction (XRD) and CHN elemental analysis. The Cu/Mn molar ration was found by Rietveld refinement, confirming a proportion 0.8:0.2 between copper and manganese ions. In the catalytic assays, the catalyst loading, nature and loading of tertiary amine promoter were investigated using 3-nitrobenzaldehyde as model substrate. The results indicate DABCO (100 mol%) as the best promoter for the reaction in the presence of 22 mol% of catalyst. Optimized conditions were applied for several aldehydes as substrates with yields up to 87%. Results suggests higher influence of nature and position of the substituent groups on the yield and reaction time. The catalyst recyclability was also investigated and showing that the material keeps the same performance for at least 4 catalytic cycles.
We study a class of elliptic problems, involving a k-Hessian and a very fast-growing nonlinearity, on a unit ball. We prove the existence of a radial singular solution and obtain its exact asymptotic behavior in a neighborhood of the origin. Furthermore, we study the multiplicity of regular solutions and bifurcation diagrams. An essential ingredient of this study is analyzing the number of intersection points between the singular and regular solutions for rescaled problems. In the particular case of the exponential nonlinearity, we obtain the convergence of regular solutions to the singular and analyze the intersection number depending on the parameter k and the dimension d.
We consider the Dirichlet boundary problems for semilinear logarithmic equations motived by semilinear Schrödinger equations and its fractional approximations.
This article is concerned with long-time dynamics for a class of semilinear thermoelastic systems with variable thermal coefficient. The main results establish, under nonlinear forces with suitable conditions of growth and dissipativity, the existence, regularity and upper semicontinuity of the pullback attractors with respect to the coefficients of thermal expansion of the material.
This work deals with the existence of solutions for a class of Hartree–Fock type system in the two dimensional Euclidean space. Our approach is variational and based on a minimization technique in the Nehari manifold. The main steps in the prove are some trick estimates from the sign-changing logarithm potential in an appropriate subspace of H1(R2)\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$H^1({\mathbb {R}}^2)$$\end{document} introduced by Stubbe [23] (see also Cingolani-Weth [8]).
This research studied the hydration of C3S-C3A-calcium sulfate systems made of combinations of two C3S (pure triclinic and Al-doped monoclinic), two C3A (pure cubic C3A and Na-doped orthorhombic), and two calcium sulfates (gypsum and hemihydrate). For each system, the hydration of four different SO3 contents (0.25-2.0 wt%) was assessed by calorimetry. The optimum SO3 content was fixed from calorimetry results, and the mixtures were evaluated by in-situ XRD and TGA. The type of C3S was the factor that most affected the sulfate balance of the systems. The mixes with Al-C3S produced a higher amount of ettringite in the first hours, resulting in much earlier sulfate depletions when compared to the mixes with pure C3S. The mixes with ort-C3A also showed faster sulfate depletion due to its higher reactivity compared with cb-C3A. Finally, the replacement of gypsum by hemihydrate resulted in faster sulfate depletion caused by the faster hemihydrate dissolution.
The biogenic amines, especially tryptamine (Tryp) and histamine (Hist), are toxicologically precarious for human health, causing intoxication. The combination of Tryp and Hist together presents even greater damage to human health, since Tryp increases the toxicity of Hist. Information about the presence of this amine is necessary to assess the quality of the wine and avoid intoxication caused by its ingestion. In this work, the electrocatalytic oxidation of Tryp and Hist on a carbon paste electrode modified with nickel phthalocyanine (CPE-NiPC) was studied, and a square wave voltammetric method was developed for simultaneous determination of these amines in wines. The use of NiPC contributed significantly to the peak current gain in the analytical response of the amines. The optimal response and sensitivity of the two analytes were obtained using phosphate buffer (pH 8.5), yielding a linear response range of 0.49–3.93 ng mL⁻¹ and 0.37–2.9 µg mL⁻¹, a limit of detection of 0.168 ng mL⁻¹ and 0.038 µg mL⁻¹ for Tryp and Hist, respectively. The proposed sensor CPE-NiPC has high selectivity affording greater accuracy in determining those amines and providing recovery values in a range of 82.65–103.02% for the analyzed biogenic amines.
Objective Ouabain, an inhibitor of Na+/K+-ATPase, is a type of endogenous hormone synthesized in the adrenal cortex and hypothalamus. Previous studies found that ouabain potently inhibited acute inflammatory reactions such as type 2 inflammation and regulated immunological processes. In this study, we aimed to investigate ouabain effect on allergic asthma. Methods: BALB/c mice were submitted to chronic airway allergic inflammation induced by an ovalbumin (OVA) protocol. The animals were treated with ouabain or standard drug, budesonide. The following parameters were evaluated: cell migration, cytokine profile, IgE levels, lung histological modifications and MAPK activation. Results: At first, it was observed that ouabain reduced OVA-induced cell migration into the lung, observed by bronchoalveolar lavage fluid (BALF) cell counting and lung histological analysis (HE stain). Additionally, ouabain negatively modulated alarmins (IL-33 and TSLP), Th2 high cytokines levels (IL-1β and IL-4) and tissue remodeling markers such as TNF-α and TGF-β. Treatment with ouabain also reduced OVA-specific IgE titers in BALF and serum, respectively, when compared to the OVA group. Lung histological parameters, including collagen deposition and mucus production induced by OVA were also attenuated by ouabain treatment. Finally, our results showed that p38 mitogen-activated protein kinase (MAPK) signaling pathways were suppressed by ouabain in this model. All these parameters were reduced by budesonide, a steroidal anti-inflammatory standard drug. Conclusion: These data together suggest that, in addition to its acute anti-inflammatory action, ouabain is also able to modulate allergic asthma.
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