Temasek Polytechnic

Preclinical studies have shown that the blood from female mice exposed weekly to magnetic fields inhibited breast cancer growth. This double-blind randomized controlled trial investigated whether analogous magnetic therapy could produce similar anticancer sera from human subjects. Twenty-six healthy adult females (ages 30–45) were assigned to either a magnetic therapy group, receiving twice weekly 1 mT magnetic exposures (10 min/session) for 4 weeks, or a control group, who underwent identical sham exposure. Blood sera were evaluated for their capacity to modulate breast cancer-related cellular responses and epithelial–mesenchymal transition. The sera from the magnetic therapy group subjects exhibited significant anticancer effects that were strongest one month after the last magnetic exposure, whereas the sera from unexposed females or unexposed or exposed males showed no effect. Female sera from the magnetic therapy group (n = 12) reduced breast cancer cell proliferation (16.1%), migration (11.8%) and invasion (28.2%) and reduced the levels of key EMT markers relative to the control sera (n = 14). Magnetic therapy modulated the serum levels of angiogenic and myogenic biomarkers in a manner consistent with improved cancer management. Muscle-targeted magnetic therapy holds the potential to enhance the anticancer properties of human blood via an adaptive process, akin to exercise training.

As social and cultural changes and the evolution of audience aesthetics, traditional crosstalk is also facing the need to update its content and form. By means of synthesis and theoretical analysis, this paper explores that while inheriting the essence of traditional crosstalk, crosstalk as an art form needs to be adapted to the needs of modern audiences through innovations in jokes and character relationships, in order to adapt to the innovation and development of modern society. While analyzing the core elements of traditional crosstalk, including the use of jokes, the construction of character relationships, and the reflection of social and cultural backgrounds, the paper points out the limitations that these elements face in modern times, such as the timeliness of the content, the rigidity of the form, the regionality of the language, the singularity of the themes, and the conservatism of performance. On this basis, the essay proposes a series of innovative strategies to evolve traditional crosstalk while meeting the contemporary audience's aesthetic needs, including content reflecting the spirit of the times, breakthroughs in traditional performance forms, overcoming linguistic locality, expanding thematic diversity, innovative performance styles, and strengthening interactivity with the audience

Introduction Students pursuing science, technology, engineering, and math (STEM) majors often struggle with essential skills critical to their academic success and future careers. Traditional self-regulated learning (SRL) training programs, while effective, require significant time investments from both students and instructors, limiting their feasibility in large lecture-based STEM courses. Methods This study investigates whether completion of three AI-powered virtual-human training modules—focused on planning, self-monitoring, and reflection—leads to increased use of corresponding MS Planner tools among STEM majors compared to a control group. Results Results indicate that students who did not complete the first two training modules were less likely to use MS Planner features for planning and self-monitoring; however, the reflection module did not yield comparable results. Discussion These findings highlight the potential of AI-powered virtual-human training as a scalable solution to enhance desirable learning behaviors among STEM majors, particularly in large and diverse classrooms. This research contributes to the understanding of effective interventions for fostering SRL behaviors in STEM education and suggests avenues for future refinement and implementation of digital training tools.

Threshold switching (TS) memristors are promising candidates for artificial neurons in neuromorphic systems. However, they often lack biological plausibility, typically functioning solely in an excitation mode. The absence of an inhibitory mode limits neurons’ ability to synergistically process both excitatory and inhibitory synaptic signals. To address this limitation, we propose a novel memristive neuron capable of operating in both excitation and inhibition modes. The memristor’s threshold voltage can be reversibly tuned using voltages of different polarities because of its bipolar TS behavior, enabling the device to function as an electronically reconfigurable bi-mode neuron. A variety of neuronal activities such as all-or-nothing behavior and tunable firing probability are mimicked under both excitatory and inhibitory stimuli. Furthermore, we develop a self-adaptive neuromorphic vision sensor based on bi-mode neurons, demonstrating effective object recognition in varied lighting conditions. Thus, our bi-mode neuron offers a versatile platform for constructing neuromorphic systems with rich functionality.

Paper presented as oral presentation (Academic Paper) at the Health Innovation Community (HIC) Conference, Session 207: Digital competence within the workforce. Held at the Brisbane Convention Centre, Room M5&6, Brisbane, Australia. Organized by the Australasian Institute of Digital Health (AIDH). [Citation: Ho J, Toh P, Yap KY. A digital health humanities approach to the three-body problem in healthcare education: Balancing the science, art and tech with M.A.G.I.C. Stud Health Technol Inform. Sep 2024; 318: 72-77.]

Art therapy fosters emotional healing and growth. This process can offer healthcare professionals (HCPs) novel insights into patients’ medication experiences. We developed a Metaverse Art Gallery of Image Chronicles (MAGIC), which depicted patients’ medication experiences symbolically as hero–villain portrayals. This gallery aimed to enhance healthcare students’ learning through relatable insights into patients’ medication therapies. A character sheet was used to craft patients’ personifications of their medication experiences through an art-based narrative therapy approach. ChatGPT, NightCafe, Canva, HeyGen, and Camtasia were used to generate hero–villain portraits based on the character traits and mounted in MAGIC, which consisted of three virtual realms, each with a unique theme. Alpha-testing among sixteen Generation Z healthcare learners indicated that the content in MAGIC enabled them to understand the concepts of medication adherence (93.7%), art therapy (87.5%), and how patients related to their medications (81.3%). Perceived playfulness (rs = 0.925, p < 0.001), perceived compatibility (rs = 0.890, p < 0.001), and social norm (rs = 0.862, p < 0.001) were strongly associated with their behavioral intention to adopt MAGIC as an educational platform. The learners enjoyed their experience (6.31 ± 0.70), felt that MAGIC was interactive and engaging (6.25 ± 0.78), and had the potential to be more effective than traditional learning methods (5.94 ± 0.93). Furthermore, they would recommend it to others for their education (5.94 ± 0.85).

Zinc‐air batteries (ZABs) have attracted considerable attention for their high energy density, safety, low noise, and eco‐friendliness. However, the capacity of mechanically rechargeable ZABs was limited by the cumbersome procedure for replacing the zinc anode, while electrically rechargeable ZABs suffer from issues including low depth of discharge, zinc dendrite and dead zinc formation, and sluggish oxygen evolution reaction, etc. To address these issues, we report a hybrid redox‐mediated zinc‐air fuel cell (HRM‐ZAFC) utilizing 7,8‐dihydroxyphenazine‐2‐sulfonic acid (DHPS) as the anolyte redox mediator, which shifts the zinc oxidation reaction from the electrode surface to a separate fuel tank. This approach decouples fuel feeding and electricity generation, providing greater operation flexibility and scalability for large‐scale power generation applications. The DHPS‐mediated ZAFC exhibited a superior peak power density of 0.51 W/cm² and a continuous discharge capacity of 48.82 Ah with ZnO as the discharge product in the tank, highlighting its potential for power generation.

Zinc‐air batteries (ZABs) have attracted considerable attention for their high energy density, safety, low noise, and eco‐friendliness. However, the capacity of mechanically rechargeable ZABs was limited by the cumbersome procedure for replacing the zinc anode, while electrically rechargeable ZABs suffer from issues including low depth of discharge, zinc dendrite and dead zinc formation, and sluggish oxygen evolution reaction, etc. To address these issues, we report a hybrid redox‐mediated zinc‐air fuel cell (HRM‐ZAFC) utilizing 7,8‐dihydroxyphenazine‐2‐sulfonic acid (DHPS) as the anolyte redox mediator, which shifts the zinc oxidation reaction from the electrode surface to a separate fuel tank. This approach decouples fuel feeding and electricity generation, providing greater operation flexibility and scalability for large‐scale power generation applications. The DHPS‐mediated ZAFC exhibited a superior peak power density of 0.51 W/cm2 and a continuous discharge capacity of 48.82 Ah with ZnO as the discharge product in the tank, highlighting its potential for power generation.

As the global demand for food increases, aquaculture plays a key role as the fastest growing animal protein sector. However, existing aquafeeds contain protein ingredients that are not sustainable under current production systems. We evaluated the use of microbial community-based single cell protein (SCP), produced from soybean processing wastewater, as a partial fishmeal protein substitute in juvenile Asian seabass (Lates calcarifer). A 24-day feeding trial was conducted with a control fishmeal diet and a 50% fishmeal replacement with microbial community-based SCP as an experimental group, in triplicate tanks containing 20 fish each. Both diets met the protein, essential amino acids (except for lysine), and fat requirements for juvenile Asian sea bass. The microbial composition of the SCP was dominated by the genera Acidipropionibacterium and Propioniciclava, which have potential as probiotics and producers of valuable metabolites. The growth performance in terms of percent weight gain, feed conversion ratio (FCR), specific growth rate (SGR), and survival were not significantly different between groups after 24 days. The experimental group had less variability in terms of weight gain and FCR than the control group. Overall, microbial community-based protein produced from soybean processing wastewater has potential as a value-added feed ingredient for sustainable aquaculture feeds.

Vision sensors are becoming increasingly ubiquitous, and they continuously collect, store, communicate, and process vast amount of sensitive data that are vulnerable to being stolen and misused. Existing cryptosystems based on complex cipher algorithms generally require extensive computational resources, making them difficult to use in vision sensors that have limited processing capabilities. Here, we propose and experimentally demonstrate a novel in situ image cryptography scheme based on a neuromorphic vision sensor comprising all-optically controlled (AOC) memristors. Due to the unique light wavelength and irradiation history-dependent bidirectional persistent photoconductivity of AOC memristors, a visual image can be stored, encrypted, decrypted, denoised, and destroyed within a vision sensor. A decrypted image can be encoded in situ and then accurately recognized through a memristive neural network. Encrypted and destroyed images are capable of withstanding hacking attacks even with trained neural networks. Our cryptography scheme enables complete cryptographic operations entirely on a sensor and, therefore, effectively safeguards visual information. This work provides a simple yet efficient solution to the security challenges faced by vision sensors.

Excessive salt intake is a risk factor that leads to cardiovascular diseases. Public education on healthy eating habits and maintaining a healthy lifestyle can enable consumers to make better informed choices. This study developed a Metaverse Escape Room to increase public awareness on the “War Against Salt”. Known as “The Saltomachy War” (TSW), 29 participants played the game in teams to solve puzzles across five “worlds” (rooms) to “escape” the game. Median post-game knowledge quiz scores were significantly higher than the pre-game quiz scores (12/15 versus 9/15, p<0.001). All players agreed that the content in TSW was useful in daily life (100%). TSW was easy to navigate (93.1%) and majority were engaged and actively participated in gameplay (96.6%). The storyline was creative and interesting (93.1%). Almost all players liked TSW to be in their curriculum and would recommend TSW to friends and family (96.6% each).

The processing of traditional Chinese medicine (TCM) is a unique traditional pharmaceutical technology in China, which is the most important feature that distinguishes Chinese medicine from natural medicine and plant medicine. Since the record in Huangdi Neijing (Inner Canon of the Yellow Emperor), till now, the processing of TCM has experienced more than 2000 years of inheritance, innovation, and development, which is a combination of TCM theory and clinical practice, and plays an extremely important position in the field of TCM. In recent years, as a clinical prescription of TCM, Chinese herbal pieces have played a significant role in the prevention and control of the COVID-19 and exhibited their unique value, and therefore they have become the highlight of China's clinical treatment protocol and provided Chinese experience and wisdom for the international community in the prevention and control of the COVID-19 epidemic. This paper outlines the research progress in the processing of representative TCM in recent years, reviews the mechanism of the related effects of TCM materials after processing, such as changing the drug efficacy and reducing the toxicity, puts forward the integration and application of a variety of new technologies and methods, so as to reveal the modern scientific mystery of the processing technology of TCM.

In this work, we introduce a novel technique that optimizes the design and properties of support structures for Powder Bed Fusion–Laser-Based Metal (PBF-LB/M) processes. The goal is to significantly ease support removal during post-processing without compromising print quality. We propose an additive manufacturing (AM) workflow that streamlines the end-to-end AM process through the creation and integration of innovative support systems, modeling software, and efficient support removal techniques. This approach enhances productivity and minimizes trial and error between design and 3D printing. Our key concept revolves around developing a “soft” support system, which can be easily removed using sandblasting. Compared to manual or machining removal, this method substantially reduces cost and time, particularly in areas with limited or no access. For locations with steep overhangs and large cross-sectional areas, the traditional strong support system can be implemented as a “hard” support. The proposed workflow allows for the development of a hybrid system combining hard and soft supports, effectively manufacturing parts with minimal design constraints. The workflow begins from a standard operating procedure (SOP) for evaluating the appropriate printing parameters to achieve a support that can be soft enough to be sandblasted for removal, yet sufficient strong to support the overhang part structure and prevent distortion. A simulation platform is developed to assess the ideal placement and combination of the hybrid support design, achieving a balance between post-processing cost and effort, and print quality. The development of this solution truly reflects the advantage of metal 3D printing technology, i.e., design freedom.

An innovative nasal filter was tested, based on aerodynamic air filtration and not on conventional air filtration by means of mesh filters. A custom testing system was designed and three sizes of the filter have been tested vs. monodispersed SiO 2 particles sized 5 μm, 1 μm, and 0.5 μm under cycling flow of 6 liters per minute, provided by an artificial lung breather simulating spontaneous breathing. Accelerated testing was implemented, challenging filters with a maximum load of 200 mg per cubic meter. All three filters' sizes showed initial filtration efficiencies above 90% vs. all particles' sizes, decreased to not less than 80% after 30 min of accelerated testing, corresponding to 4.5 days of continuous use at 2 mg challenge, this value being associated with hazardous air conditions in the PSI scale. Results in this study indicate that nasal filters based on aerodynamic air filtration can provide fine and ultrafine filtration, offering protection in day-to-day life from risks associated with pollens, mites, PM, pollutants, and respiratory infectious agents, introducing acceptable respiratory resistance.

Introduction Immune checkpoint blockade (ICB) is a systemic therapeutic option for advanced hepatocellular carcinoma (HCC). However, low patient response rates necessitate the development of robust predictive biomarkers that identify individuals who will benefit from ICB. A 4-gene inflammatory signature, comprising CD8, PD-L1, LAG-3, and STAT1, was recently shown to be associated with a better overall response to ICB in various cancer types. Here, we examined whether tissue protein expression of CD8, PD-L1, LAG-3, and STAT1 predicts response to ICB in HCC. Methods HCC samples from 191 Asian patients, comprising resection specimens from 124 patients (ICB-naïve) and pre-treatment specimens from 67 advanced HCC patients treated with ICB (ICB-treated), were analyzed for CD8, PD-L1, LAG-3, and STAT1 tissue expression using multiplex immunohistochemistry followed by statistical and survival analyses. Results Immunohistochemical and survival analyses of ICB-naïve samples showed that high LAG-3 expression was associated with shorter median progression-free survival (mPFS) and overall survival (mOS). Analysis of ICB-treated samples revealed that high proportions of LAG-3⁺ and LAG-3⁺CD8⁺ cells pre-treatment were most closely associated with longer mPFS and mOS. Using a log-likelihood model, adding the total LAG-3⁺ cell proportion to the total CD8⁺ cell proportion significantly increased the predictive values for mPFS and mOS, compared with the total CD8⁺ cell proportion alone. Moreover, levels of CD8 and STAT1, but not PD-L1, were significantly correlated with better responses to ICB. After analyzing viral-related and non-viral HCC samples separately, only the LAG3⁺CD8⁺ cell proportion was significantly associated with responses to ICB regardless of viral status. Conclusion Immunohistochemical scoring of pre-treatment levels of LAG-3 and CD8 in the tumor microenvironment may help predict ICB benefits in HCC patients. Furthermore, immunohistochemistry-based techniques offer the advantage of being readily translatable in the clinical setting.

An innovative nasal filter was tested, based on aerodynamic air filtration and not on conventional air filtration by means of fibrous filters. A custom testing system has been designed and three sizes of the filter have been tested vs. monodispersed SiO 2 particles sized 5 µm, 1 µm and 0.5 µm, under cycling flow of 6 litres per minute, provided by an artificial lung breather simulating spontaneous breathing. Accelerated testing was implemented, challenging filters with a maximum load of 200 mg per cubic meter. All three filters’ sizes showed initial filtration efficiencies above 90% vs. all particles’ sizes, decreased to not less than 80% after 30 minutes of accelerated testing, corresponding to 4.5 days of continuous use at 2 mg challenge, this value being associated with hazardous air conditions in the PSI scale. Results in this study indicate that nasal filters based on aerodynamic air filtration can provide fine and ultrafine filtration protecting population in day-to-day life from risks associated to pollens, mites, PM, pollutants and respiratory infectious agents.

Tuberculosis (TB) is a public health challenge globally, and molecular testing is recommended to expedite diagnosis. Concerns that Xpert MTB/RIF assay (Xpert) may be less sensitive when testing paucibacillary samples led to the development of the Xpert MTB/RIF Ultra assay (Ultra). We evaluated the performance of Ultra against Xpert on clinical samples sent to the national reference laboratory in Singapore. In total, 149 samples collected between January 2019 and November 2020 were analysed. Mycobacterium tuberculosis complex (MTBC) was isolated from 55 cultures. Using culture as the reference standard, Ultra demonstrated higher sensitivity (96.4% vs 85.5%) and marginally lower specificity (88.3% vs 89.4%) compared to Xpert in the full cohort. When considering only paucibacillary specimens such as extrapulmonary and smear-negative samples, similar results were obtained. Reclassifying Ultra trace results (low levels of MTB are detected but no rifampicin resistant result is detected) as negative in the full cohort led to a decrease in sensitivity by 10.9% and a marginal increase in specificity by 1.1%. In instances of low bacillary load, Ultra also identified rifampicin resistance more accurately than Xpert, when corroborated against other methods such as broth microdilution, line probe assay and whole genome sequencing (WGS). One isolate tested rifampicin-resistant using Xpert and Ultra, but was phenotypically susceptible and WGS demonstrated the presence of the silent mutation Thr444Thr. Ultra is more sensitive than Xpert in the detection of MTBC and rifampicin resistance in our local setting. Nevertheless, the results of molecular testing should still be correlated with phenotypic studies.

Background Improved survival of neonates with esophageal atresia with/without tracheoesophageal fistula (EA/TEF) has resulted in increased prevalence of gastro-esophageal and respiratory-related morbidities. However, long-term outcome data on these patients remains limited, making it difficult to substantiate any guidelines on their chronic care. The purpose of our study is to report on their post-operative outcomes especially the long-term gastro-esophageal and respiratory morbidities. Methods This was a retrospective review of 65 patients (39 males, 26 females) who underwent EA/TEF repair from 1996 to 2019 at a single tertiary institution. Follow up data pertaining to clinical characteristics, operative management and long-term gastro-esophageal and respiratory morbidities was analyzed. Results Fifty-seven patients (87.7%) had Gross Type-C anatomy, followed by 5(7.7%) patients with Type-A, 1(1.5%) with Type-B and 1 with Type-D. One patient had a late diagnosis of H-type fistula (Type-E). Thirteen (20%) patients had long-gap EA. Median age at first surgery was day 1 (IQR 1–2) of life. All patients underwent bronchoscopy at their index surgery. All 52 non-long gap EA (LGEA) patients underwent primary anastomosis, while most (76.9%) LGEA patients underwent staged repair. Post-operatively, 4(6.2%) developed anastomotic leak which resolved with conservative management. Three (4.6%) had recurrent TEF, 2 underwent re-do ligation. Twenty (30.8%) patients developed anastomotic strictures, with 15 requiring serial dilatation. Long-term burden of gastro-esophageal and respiratory morbidity was high (63.1%; 64.6% respectively). The majority ( n = 39,60%) of patients required active follow-up for a median duration of 5 years (IQR 1.5–10 years). Predominant conditions were gastroesophageal reflux disease ( n = 28, 43.1%), dysphagia ( n = 20, 30.8%), recurrent respiratory infections ( n = 23, 35.3%), chronic cough ( n = 19, 29.2%), and pneumonia ( n = 19, 29.2%). Tracheomalacia was diagnosed in 22(33.8%), 2 of whom required tracheostomy for severe disease. Overall mortality rate was 10.8% ( n = 7): 5 demised due to chronic respiratory failure, while 2 demised intra-operatively during the index surgery. Conclusion Despite successful surgical repair for EA/TEF, our data demonstrated significant morbidities among EA/TEF survivors, thus highlighting the importance of long-term multi-disciplinary care with collaboration between respiratory, gastroenterology, and otolaryngology specialists. Level of evidence Prognostic, Level IV.