National Research Center, Egypt
Recent publications
Different 2,4-thiazolidinedione-tethered coumarins 5a–b, 10a–n and 11a–d were synthesised and evaluated for their inhibitory action against the cancer-associated hCAs IX and XII, as well as the physiologically dominant hCAs I and II to explore their selectivity. Un-substituted phenyl-bearing coumarins 10a, 10 h, and 2-thienyl/furyl-bearing coumarins 11a–c exhibited the best hCA IX (KIs between 0.48 and 0.93 µM) and hCA XII (KIs between 0.44 and 1.1 µM) inhibitory actions. Interestingly, none of the coumarins had any inhibitory effect on the off-target hCA I and II isoforms. The sub-micromolar compounds from the biochemical assay, coumarins 10a, 10 h and 11a–c, were assessed in an in vitro antiproliferative assay, and then the most potent antiproliferative agent 11a was tested to explore its impact on the cell cycle phases and apoptosis in MCF-7 breast cancer cells to provide more insights into the anticancer activity of these compounds.
Background Nanopesticides are considered promising strategy in pest control. So, this strategy became an important new approach in pesticide formulations production. In this study, three traditional pesticide formulations (chlorfenapyr, imidacloprid and indoxacarb) were developed into nanoformulations. The toxicity of these nanoformulations was evaluated against the adults of the conical snail, Cochlicella a cuta (Müller, 1774), and the chocolate banded snail, Massylaea vermiculata (Mohamed and Ali, Anim Biol J 2(4):171–180, 2011). Results The results revealed that the nanosizes of nanoformulation nanoparticles were ranged between 220 and 534 nm. The loading capacities of the selected pesticides on polymer were 65.3 ± 6.4, 62.8 ± 5.3 and 37.1 ± 0.4%for nanochlorfenapyr, nanoimidacloprid and nanoindoxacarb, respectively. The nanochlorfenapyr was the most toxic against both the C. acuta and M. vermiculata followed by nanoindoxacarb and nanoimidacloprid. The LC 50, s of the nanoformulations were 6.1, 7.7 and 14.9 ppm for chlorfenapyr, indoxacarb and imidacloprid, respectively. Conclusions These results cleared that the conical snails were more susceptible to all the tested pesticides than the chocolate banded snail, M. vermiculata . The efficacy of nanochlorfenapyr, nanoindoxacarb and nanoimidacloprid on conical snails (6.1, 7.7 and 14.9 ppm, respectively) was about fourfold compared with M. vermiculata (23.1, 31.9 and 60.6 ppm, respectively) . The results also revealed that the potency of nanoformulation used was not only by direct killing of tested snails but also by repellent effect.
Background Introducing new crops to reduce the nutritional gap in Egypt is an important target. One of these crops is Quinoa which is characterized by high nutritional value with multiple food uses. However, it will compete with other winter crops, so it is candidate to grow in marginal lands like calcareous soils. Meanwhile, planting density and N requirements are not known under such conditions; therefore, this work was undertaken in calcareous soil Caco 3 > 50%. Methods The experiments included three nitrogen fertilization treatments (0, 50 and 100 kg/fed.) and two plant densities (20,000 plants/fed. and 36,000 plants/fed.) on Quinoa ( Chenopodium quinoa Willd.) cv. Shibaya Field over two consecutive winter seasons of 2018/2019 and 2019/2020 in calcareous soil. Quinoa seed yield, biological yield, straw yield, weight of 1000-seed, harvest index, total nitrogen percentage in seeds, crude protein content in seeds, phosphorus content in seeds and potassium percentage in seeds were determined. The experimental design was a split plot design with three replications. Results The results showed that applying nitrogen fertilization at a rate of 100 kg/fed. had significantly greater seed yield, biological yield, seed yield, straw yield and weight of 1000-seed. All seed chemical contents except K percentage were significantly affected due to nitrogen fertilization. Increasing plant density from 20,000 to 36,000 plants per feddan significantly increased the biological yield, seed yield, straw yield, weight of 1000-seed and harvest index. The interaction between the two studied factors was significant on all characters of this investigation except, K percentage in seeds. Conclusion It can be concluded from this study that the quinoa crop responds to the increase in nitrogen fertilization and increases productivity and quality when fertilized with a rate of 100 kg nitrogen per fed.
Background No doubt that the corpus luteum (CL) plays a vital role in the regulation of female cyclicity in mammals. The scenarios among microRNAs (miRNAs) and their target genes and steroid hormones {estradiol (E2) and progesterone (P4)} are required for better understanding the molecular regulation of CL during its formation, maturation, and regression. We aimed to (I) study the changes in the relative abundance of miR-205, miR-26a-5p, miR-17-5p, and let-7b-5p and their target genes: LHCGR, CASP3, PCNA, AMH, and PLA2G3, during different stages of corpus luteum in Egyptian buffaloes, and (II) and to address different scenarios between steroid concentrations in the serum and the expression pattern of selected miRNAs and their targets. Methods The paired ovaries and blood samples were collected from apparently healthy 50 buffalo cows at a private abattoir. The ovaries bearing CL were macroscopically divided according to their morphological structure and color into hemorrhagic (CLH), developing (CLD), mature (CLM), regressed (CLR), and albicans (CLA). Small pieces from different stages of CL (CLH, CLD, CLM, CLR, and CLA) were cut and immediately kept at − 80 °C for total RNA isolation and qRT-PCR. The serum was separated for steroid level estimation. Results The LHCGR was expressed during different stages of CL, and the peak of expression was at the mid-luteal stage. The CASP3 revealed a stage-specific response at different stages of CL. The PCNA has an essential role in cellular proliferation in buffaloes CL. Both expression patterns of PLA2G3 and AMH were found over the various developmental and regression stages. It was noticed that miR-205 is conserved to target LHCGR and CASP3 transcripts. Moreover, CASP3 and AMH were targeted via miR-26a-5p. Additionally, the CASP3 and PLA2G3 were targeted via let-7b-5p . The P4 level reached its peak during CLM. There were positive and negative strong correlations between miRNAs (miR-26a-5p and miR-205), target genes (LHCGR and CASP3) during different stages of CL, and steroid hormones in the serum. Conclusions Taken together, the orchestrated pattern among miRNAs, target genes, and steroid hormones is essential for maintaining the proper development and function of CL in buffalo cows.
Background Diarrhoea is still a major public health issue in developing countries, and it is one of the leading causes of morbidity and mortality in children. We aimed to assess the use of a multiplex reverse transcription polymerase chain reaction (RT-PCR) assay for the detection of five viruses, including rotavirus, norovirus (genogroups 1 and 2), astrovirus, and adenovirus, responsible for gastroenteritis in children under 5 years old in primary care centres in Upper Egypt. Subjects and methods A total of 500 stool samples were collected. Fifty samples were randomly selected for viral examination using multiplex RT-PCR for the detection of rotavirus , norovirus (genogroups 1 and 2), astrovirus, and adenovirus, causing diarrhoea. Results Viruses were detected in 45 (90%) of the 50 stool samples. The most frequently identified virus was norovirus G2, followed by Group A rotavirus, astrovirus and adenovirus. Mixed infection by two and three viruses was observed in 7/50 cases (14%) and 2/50 cases (4%), respectively. Norovirus G1 was not detected in the samples examined. Conclusion Our study reveals that multiplex PCR allows for the detection of multiple viral targets in only one reaction, rendering the assay easier to perform compared to existing testing methodologies (RT-PCR and electron microscopy). Additionally, most of the viruses were detected in summer, and the highest prevalence was in the age group less than 1 year. Norovirus G2 and rotavirus were the most frequent agents and the most common coinfections responsible for gastroenteritis in children.
Background Recovery of valuable ingredients from black liquor could lead to an environmentally and economically sound bioethanol production technology. In this work, two schemes comprising hybrid membrane systems incorporating ultrafiltration (UF) and nanofiltration (NF) are developed for the recovery of lignin, silica rich and cellulose/hemicellulose hydrolysates byproducts from alkaline pretreated rice straw. Methods The first scheme (I) comprises UF, NF and thermal vapor compression (TVC), while, the second scheme (II) includes UF, 2 stages of NF and 2 TVC units. Further treatments are suggested to produce solid byproducts with an economic value. Furthermore, material balance of the two schemes based on 1000 m ³ /d of black liquor and the main design features and comparative direct cost indicators of the main adopted units were deduced using WT Cost II© software. Results Results revealed that about (80–90%) yield of recovered byproducts from both schemes with equivalent amounts of 9.5, 5.5 and 18.5 ton/d of lignin, silica rich and cellulose/hemicellulose hydrolysates dry products, respectively. Moreover, reusable water recovery approaches 26% and 70% for schemes (I) and (II), respectively. Conclusions Further, the wastewater generated from scheme (II) is 2.9 times folds scheme (I) which improves the environmental impact of the former. Preliminary cost indicators revealed that both schemes have almost the same total direct capital cost.
Background and aims Gestational diabetes mellitus is well-defined as glucose intolerance first documented during pregnancy. In this study, we examined the possible associations between I/D polymorphism of the angiotensin-converting enzyme gene, the M235T variant of angiotensinogen gene, and the rs7950226 polymorphism of the ARNT -like protein-1 ( BMAL1 ) gene and the risk for diabetes in Egyptian pregnant women. Subjects and methods This study recruited 160 gestational diabetes cases and 165 controls. Genomic DNA was derived from peripheral blood leukocytes and ACE gene (I/D) genotyping was performed using the method of polymerase chain reaction and the polymerase chain reaction-based restriction fragment length polymorphism was used for identifying the M235T variant of AGT gene and the rs7950226 polymorphism of the BMAL1 . Results The II, ID, and DD genotypes of the ACE gene have significant differences in cases compared to controls ( P = 0.000 and X ² = 81.77). The M235T polymorphism of the AGT gene was increased with gestational diabetes risk. Furthermore, the AA genotype of the BMAL1 rs7950226 gene was significantly related to the gestational diabetes risk ( P = 0.000 and X ² = 52.82). Furthermore, the allele frequencies of the three variants have significant variances between cases and control. Conclusion This study suggested significant associations between ACE (DD), AGT (TT), and BMAL1 rs7950226 (AA) gene polymorphisms with gestational diabetes susceptibility and there was a possibility to identify that II + MM + GG as protective haplotypes and DD + TT + AA as risk haplotypes for gestational diabetes.
Background Methyl CpG binding protein 2 (MeCP2) is essential for the normal function of mature neurons. Mutations in the MECP2 gene are the main cause of Rett syndrome (RTT). Gene mutations have been identified throughout the gene and the mutation effect is mainly correlated with its type and location. Methods In this study, a series of in silico algorithms were applied for analyzing the functional consequences of 3 novel gene missense mutations (D121A, S359Y, and P403S) and a rarely reported one with suspicious effect (R133H) on RettBASE. Besides, a ROC curve analysis was performed to investigate the critical factors affecting variant pathogenicity. Results (1) The ROC curve analysis for a retrieved set of MeCP2 variants showed that physicochemical characters do not significantly affect variant pathogenicity; (2) PREM PDI tool revealed that both D121A and R133H mainly contribute to disease progression via reducing MeCP2 affinity to DNA; (3) GPS v5.0 software indicated that P403S may correlate with altered protein phosphorylation; however, no defective protein interaction has been already documented. (4) The applied computational algorithms failed to explore any informative pathogenic mechanism for the S359Y variant. Conclusion The conducted approach might provide an efficient prediction model for the effect of MECP2 variants that are located in MBD and CTD.
Due to the scarcity of concentrate and green fodder and its high prices in animal feeding, the quest for readily available and inexpensive alternative sources has become critical for breeders seeking to minimize the cost of animal production. Azolla is an invasive plant that grows faster in freshwater lakes, rivers, wetlands, and ditches in both temperate and tropical countries by a few precautionary steps with low production costs. It can modify biodiversity and aquatic ecosystems substantially. Azolla has a symbiotic relationship with the nitrogen-fixing blue-green alga Anabaena azollae , which increases the protein content of Azolla, making it one of the greatest alternative feed ingredients as a source of protein, with 25–30% protein, 7–10% amino acids, vitamins (Beta Carotene, vitamin A, vitamin B12), minerals (calcium, potassium, phosphorus, ferrous, magnesium, copper, etc.), and antioxidants. Several studies showed that Azolla can be fed to animals like cow, buffalo, sheep, goat and rabbit because it is easily digestible (because to its high protein and low lignin content), increases feed efficiency, average daily gain of animals, and milk production by 15–20%. Therefore, the present review was aimed to show the possibility of using Azolla as a source of alternative low-cost protein and its impact on animal efficiency.
Background Down syndrome (DS) is characterized by variable degrees of intellectual disability (ID). The coronavirus disease-2019 (COVID-19) lockdown prevented children with DS from reaching their rehabilitation facilities. This could have led to deterioration of their abilities and mental health hazards. The aim of this cohort study was to investigate frequency of COVID-19, the influence of COVID-19 pandemic on health, and some abilities of children with DS, and to explore factors that could have governed receiving home-based training during the lockdown. A survey of 150 individuals with Down syndrome was answered by their caregivers. Additionally, 135 participants were subjected to assessment of cognitive, language, and motor abilities using Portage program. They were divided into 2 groups: group I who received online therapy sessions during the lockdown and group II who did not receive sessions. Logistic regression was used to determine the factors which influenced getting home-based training. Results The percentage of COVID-19 cases was 3.3%. All evaluated abilities were reduced despite receiving online sessions particularly language performance ( P < 0.001). Male gender, having severe ID and low parental education were among the factors which encouraged parents to get virtual training. Conclusion COVID-19 pandemic had a negative impact on the abilities of DS children even those who got rehabilitation sessions. Their dependence on social interaction could have limited the benefit of virtual sessions. Factors that influence a parent’s decision to get home-based training should be monitored and targeted in order to overcome obstacles or concepts that may prevent families from enduring home-based intervention.
Background The most critical part in management of children in the dental clinic is control of pain and this can be achieved through local anesthesia. When the treatment plan includes extraction of mandibular primary molars, the nerve block technique is the technique of choice for administration of local anesthesia. However, this technique is accompanied with discomfort due to deep penetration of the needle, possibility of nerve injury, hematoma as well as risk of self-inflicted trauma. Therefore, researchers have searched for an alternative technique to facilitate providing painless dental treatment for the child with avoidance of the possible complications of the nerve block technique, and this can be achieved by using a strong and deeply penetrating type of local anesthesia like articaine 4% that can be effective when administered with infiltration technique. The aim of the current study was to compare the efficacy of infiltration anesthesia using alexadricaine 4% and Mepecaine-L 2% in control of pain during extraction of lower 2nd primary molars in children and to compare the change in the heart rate that occurs as a consequence of pain during extraction. A total of 50 children were included in the current study, pain assessment was performed through measuring physiological parameter (pulse rate), subjective parameter (Wong–Baker Facial Pain Scale) and objective parameter (Sound Eye Motor scale). Results showed that there was significant increase in the pulse rate in both groups, while extraction using alexadricaine showed significantly lower pain scores either with Wong–Baker Facial Pain Scale (WBFPS) or with Sound Eye Motor scale (SEM). Conclusions Using a potent anesthetic solution like alexadricaine 4% facilitates extraction of mandibular primary molars, and achieves efficient pain control, with infiltration technique only without any need for the invasive nerve block technique.
Background Increasing cultivation of strawberry in Egypt has boosted efforts to increase its yield. Biocontrol agents (BCAs) may avoid side effects and health risks caused by chemical fungicides used to control black root rot disease in strawberry. Some BCAs control the disease and augment strawberry yield, but additional research is needed to fit BCAs into emerging control strategies. The impact of six bacterial isolates of Paenibacillus polymyxa and Bacillus brevis on this disease and on berry yield is reported and compared to a common chemical fungicide. Results The bacterial isolates reduced the growth of the black root rot causal agents Fusarium solani , Rhizoctonia solani , and Macrophomina phaseolina, in dual culture bioassays. The greatest fungal inhibition was caused by P. polymyxa isolates 1 and 2 and B . brevis isolate 2. They suppressed the growth of F. solani , R. solani , and M. phaseolina by more than 74, 76, and 79%, respectively. Disease severity and incidence were significantly reduced on naturally infected strawberry plants in the field by the six isolates. The best results were obtained by the superior bioassay isolates which suppressed the disease incidence by 73, 77, and 71%, and its severity by 72, 78, and 70%, respectively. Disease suppression by bacteria was comparable to that by fungicide Actamyl. Bacteria surpassed Actamyl with regard to strawberry yield and enhancement of peroxidase and chitinase activities in the leaves. Conclusions These isolates are potential benign alternatives to fungicides used against black root rot in strawberry in Egypt. More studies are needed to examine their economic use on a wider scale.
The aim of this trial was to compare the microbial colonization of heat-cured silicone and heat-cured acrylic resin in obturators restoring acquired maxillary defects. The experiment was carried out on six partially edentulous patients having unilateral total maxillectomy defects approaching midline (class I Aramany classification) who are in need of definitive obturator. Selected patients received metal framework prosthesis with heat-cured acrylic resin bulb extending into the surgical site. At the time of delivery, a swab was obtained from the patients who represent the baseline for the group I. Patients were recalled after two and four weeks from wearing the obturator for microbiological evaluation and a swab was taken each time from the same place. In group II, the heat-cured acrylic resin was replaced with heat-cured silicone and a swab was obtained on the day of insertion as a baseline for group II. Patients were recalled after two and four weeks from the insertion of the relined obturator for microbiological evaluation and a swab was taken each time from the same place. Swabs were obtained from the nasal surface of the surgical defect and immediately cultivated into three different media Blood Agar, Sabouraud Dextrose Agar, and Macconkey media and incubated for microbiological evaluation. The identification and quantification of the isolated microorganisms were performed using the conventional microbiological cultivation method. Finally, the collected data were tabulated and statistically analyzed. Statistical analysis of the collected data showed, that the difference between the two groups was insignificant. However, patients were more satisfied with obturators lined with heat-cured silicone. It was concluded that within the limitations of this study, both acrylic resin and resilient lining materials could be used as a material for obturator construction in maxillofacial cases. However, longer follow-up period might show different results.
Background To evaluate the effect of modifying conventional glass ionomer (CGIC) and resin-modified glass ionomer (RMGIC) with nano-hydroxyapatite (Nano-HA) on their clinical performance as restorations for class V cavities. Methods The wet-chemical precipitation method was utilized to prepare the nano-hydroxyapatite particles. Nano-HA was then characterized using Scanning Electron Microscopy, Elemental Dispersive X-ray and thermal gravimetric analysis. Five wt% of the nano-hydroxyapatite (Nano-HA) was then incorporated into the powder portion of the CGIC and RMGIC. Sixty Class V cavities were prepared in thirty patients with at least two cervical caries lesions. Prepared cavities were restored using the tested materials. Modified United States Public Health Service (USPHS) criteria were used to evaluate the restorations at base line, after three, six and nine months. Results Regarding the investigated modified USPHS criteria, the tested restorative materials showed no statistically significant difference throughout the study period. While color match, surface texture and marginal integrity criteria showed a statistically significant change in nano-HA-GIC. Surface texture and marginal integrity showed a significant change with CGIC. A significant change in surface texture was only found with nano-HA-RMGIC. Conclusions The investigated restorative materials showed an equivalent clinical performance at the nine months follow-up.
Abstract Objective To compare the push out bond strength of MTA apical plug in a simulated immature root, after using different irrigation protocols; NaOCl + 20% Etidronic acid (HEBP) and NaOCl + 17% Ethylenediaminetetraacetic acid (EDTA) with and without diode laser activation. Methods Twenty-eight extracted single straight rooted permanent human teeth were selected. The root lengths were standardized (15 mm) by decronation of all samples. Instrumentation was done using the ProTaper Next system (Dentsply Sirona), the canals were flushed with 1 mL NaOCL after each instrument. Then, the apical 3 mm was cut perpendicular to the root’s long axis and the canals were prepared till file 90( master file) using the balanced force technique to simulate immature teeth. Samples were divided into four experimental groups (n = 7) in accordance with the irrigation protocol; G1 (NaOCl + HEBP), G2 (NaOCl + EDTA) both G1 and G2 were activated with diode laser, while G3 (NaOCl + HEBP) and G4 (NaOCl + EDTA) were activated with manual agitation. Canals were filled with a 5-mm MTA (ProRoot MTA, Dentsply Tulsa Dental) apical plug. Each root was transversely sectioned perpendicular to their long axis to have a 3 mm ± 0.1sections in thickness from the root apical portion. A push-out test was performed, and the failure pattern was assessed. Push out bond strength values were analyzed with student t test for compared pairs. Two-way ANOVA was used to detect the effect of each variable (chelating agent with/without laser activation). Result Effect of diode laser activation in G1 and G2 showed no significant differences (p > 0.05) on the push out bond strength values of MTA, while in manual activated groups (G3 and G4), there was a statistically significant difference (p
Background Melatonin (MEL) is a powerful antioxidant molecule with anti-androgenic property. A microemulsion (ME) system loaded with MEL was designed for treatment of androgenic alopecia. Pseudo-ternary phase diagram was constructed, and ME formulae were developed using coconut oil, Tween 80 and PEG 400. In the present study, MEL ME was characterized and evaluated for droplet size, polydispersity index, zeta potential, morphology using TEM imaging. MEL ex vivo permeation study through rat skin followed by tape stripping for stratum corneum (SC) was performed for different ME formulae, to determine skin permeation parameters and detect SC-MEL deposition. Results Spherical and uniform particles of MEL-loaded microemulsion were formulated with high stability. In ex vivo permeation study, MEL ME exhibited low steady-state skin flux along with pronounced SC deposition which prevailed a controlled release manner. Conclusion The results suggested that MEL ME could be a promising candidate for further permeation and in vivo studies for androgenic alopecia treatment.
Background This study was designed to generate functional insulin-producing cells (IPCs) from dental-derived mesenchymal stem cells (MSCs) and further explore their therapeutic potential against diabetes mellitus in vivo. MSCs were isolated from human dental pulp and periodontal ligament and were induced to differentiate into insulin-producing cells (IPCs) using laminin-based differentiation protocol for 14 days. Confirmation of IPCs was performed through real-time PCR analysis and insulin release assay. Then, the generated IPCs were labeled with PKH26 dye prior to transplantation in experimental animals. Twenty-eight days later, blood glucose, serum insulin (INS), c-peptide (CP), and visfatin (VF) levels and pancreatic glucagon (GC) level were estimated. Pancreatic forkhead box protein A2 (Foxa2) and SRY-box transcription factor 17 (Sox17), insulin-like growth factor I (IGF-1), and fibroblast growth factor10 (FGF 10) gene expression levels were analyzed. Results Dental stem cells were successfully differentiated into IPCs that demonstrated increased expression of pancreatic endocrine genes. IPCs released insulin after being subjected to high levels of glucose. In vivo findings uncovered that the implanted IPCs triggered significant decrease in blood glucose, serum VF, and pancreatic GC levels with significant increase in serum INS and CP levels. Furthermore, the implanted IPCs provoked significant upregulation in the expression level of pancreatic genes. Histopathological description of the pancreas tissues revealed that transplantation of IPCs ameliorated the destabilization of pancreas tissue architecture. Conclusion This study demonstrates the significant role of the implantation of IPCs generated from dental-derived stem cells in treatment of diabetes mellitus.
Hazelnut oil (HO) is important in terms of fatty acid composition and bioactive substances. Although there are a few studies on hazelnut oil encapsulation, there is limited research on the investigation of different wall material combinations for hazelnut oil microencapsulation and oxidative stability of the microcapsules. This study aimed to evaluate the effects of different wall material combinations (gum Arabic, sodium caseinate, whey protein, gelatine, modified starch “Em-Cap” and pea protein with maltodextrin at a ratio of 1:9,w/w, respectively) on the oxidation degree of hazelnut oil microcapsules produced by spray drying. The feed emulsions used for microcapsule production were analyzed for emulsion stability (ES) and particle droplet size. The encapsulation efficiency (EE), moisture content, bulk density, particle size and total yield of hazelnut oil microcapsules were analyzed. Peroxide and p -Anisidine values were investigated during 15 days of storage at 50 °C. While the highest encapsulation efficiency, emulsion and oxidative stability were obtained for modified starch/maltodextrin (Em-Cap/MD), the lowest yeild was obtained for gelatine/maltodextrin (GE/MD) with the highest oxidation rate. Among the six wall material combinations evaluated, the modified starch (Em-Cap) performed best, with the highest encapsulation efficiency and lowest lipid oxidation rate. The results showed that the oxidative stability of hazelnut oil microcapsules was enhanced by combining different wall materials to increase the shelf life, which is reflected at the level of the food industry.
Background Oxidative stress is postulated to have a major role in the pathophysiology of Bechet’s Disease (BD). Growing evidence suggests that vitamin D has important roles in enhancing the expression of anti-inflammatory cytokines as well as certain antioxidants. However, there is little evidence currently about the antioxidant properties of vitamin D in BD. Objective To study the relationship between vitamin D levels and the oxidative stress markers in patients with BD in addition to its association with disease activity and severity. Methods Sixty BD patients (45 males, 15 females; mean age: 34.2 ± 9.6 years) were enrolled in this study and compared to a sex and age matched control group. Plasma 25-Hydroxy vitamin D (25-OH-D) was measured using Human (25-OH-D) ELISA assay. Plasma malondialdehyde (MDA), nitric oxide (NO), reduced glutathione (GSH), superoxide dismutase (SOD) activity, catalase (CAT) activity and total antioxidant capacity (TAC) were determined by spectrophotometric methods in both groups. Plasma calcium (Ca) was measured by ELISA assay. Results When compared to controls vitamin D, GSH, CAT activity, TAC and Ca were significantly lower in BD patients, while MDA and NO levels were significantly increased in BD patients. Our Results Found that vitamin D was inversely correlated to BD current Activity form (BDCAF), disease severity score, ESR, CRP, MDA and NO, while vitamin D was significantly positively correlated to GSH, SOD, TAC and Ca. Conclusion Our study confirms that a lower level of vitamin D is associated with the oxidative stress state in BD patients as detected by MDA and NO elevation as well as decreased GSH, SOD activity, CAT activity and TAC. Hence, Vitamin D fortified foods and beverages or supplementation may improve disease severity and oxidative stress in BD patients.
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2,309 members
Ibrahim Habib
  • Analytical Chemistry Lab
Bassem El-Menshawi
  • Head, Drug Bioassay Cell Culture Laboratory, Pharmaceutical Sciences Division
Hoballah Es
  • Department of Agricultural Microbiology
Mohamed Deabes
  • Food Toxicology and Contaminants
Cairo, Egypt
Head of institution
Prof. Dr. Mohamed Hashim
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