Ministero dell'Istruzione, dell'Università e della Ricerca

In physics, thought experiments are impressive heuristic tools. They are valuable instruments to help scientists find new results and to teach students the known ones. However, as we shall show, they should always be received with prudence, even when they are a shortcut to ‘prove’ well-established results. Here, we show that the most widely known thought experiments devised to derive the gravitational frequency shift from energy conservation are, in fact, problematic. When properly set and correctly read, those thought experiments reveal that the existence of the gravitational frequency shift is, in fact, at odds with energy conservation. We also propose two new simple thought experiments, one using energy conservation and the other the conservation of linear momentum, that corroborate that conclusion, showing that those conservation principles do not imply the gravitational frequency shift. Our results may be of some epistemological interest and could serve as a warning sign on how thought experiments should be received and trusted.

Artificial neural networks (ANNs) are versatile tools capable of learning without prior knowledge. This study aims to evaluate whether ANN can calculate minute volume during spontaneous breathing after being trained using data from an animal model of metabolic acidosis. Data was collected from ten anesthetized, spontaneously breathing pigs divided randomly into two groups, one without dead space and the other with dead space at the beginning of the experiment. Each group underwent two equal sequences of pH lowering with pre-defined targets by continuous infusion of lactic acid. The inputs to ANNs were pH, ΔPaCO2 (variation of the arterial partial pressure of CO2), PaO2, and blood temperature which were sampled from the animal model. The output was the delta minute volume (ΔVM), (the change of minute volume as compared to the minute volume the animal had at the beginning of the experiment). The ANN performance was analyzed using mean squared error (MSE), linear regression, and the Bland-Altman (B-A) method. The animal experiment provided the necessary data to train the ANN. The best architecture of ANN had 17 intermediate neurons; the best performance of the finally trained ANN had a linear regression with R² of 0.99, an MSE of 0.001 [L/min], a B-A analysis with bias ± standard deviation of 0.006 ± 0.039 [L/min]. ANNs can accurately estimate ΔVM using the same information that arrives at the respiratory centers. This performance makes them a promising component for the future development of closed-loop artificial ventilators.

In this manuscript we reported the results of our studies concerning the response of vibration bands in deoxyribonucleic acid region in neuronal-like cells under exposure to static and 50 Hz electromagnetic fields using Fourier Transform Infrared spectroscopy. This technique was chosen because it is able to detect alteration of vibration bands of chemical compounds even induced by small stress agents. The aim of this study was to investigate the response of chromatin and chromosome to low intensity electromagnetic fields at values similar to manmade electromagnetic fields. The phosphate bands of asymmetric and symmetric stretching mode (representative of deoxyribonucleic acid spectral region) were observed to decrease after 3 h exposure at 1 mT (both static and 50 Hz magnetic field). Prolonging time exposure or increasing the intensity of applied magnetic field induced a low increasing in intensity of these vibration bands. This finding can be explaining assuming that chromatin uncoiling occurred at low intensity of the field and that increasing time exposure or magnetic field intensity caused an increasing of the torque on chromosome inducing an alignment along the direction of the field. ARTICLE HISTORY

School-settings represent ideal context to promote healthy habits as in adolescence most risk factors may occur or intensify leading to the adoption of unhealthy lifestyles. Thus, a deeper understanding of the factors promoting healthy lifestyles in adolescents is of utmost importance. This observational study aims to investigate: 1) gender-related differences in physical activity (Physical Activity Questionnaire for Adolescets – PAQ-A), sleep quality (Pittsburgh Sleep Quality Index - PSQI) and internet addiction (Internet Addiction Test – IAT) levels and Volition in Exercise (VE); 2) the relationship between PAQ-A and VE, PSQI and IAT; 3) the effects of a five-month (T0; T5) school-based physical activity (PA) intervention on the above-mentioned factors. PAQ-A,VE, PSQI and IAT were assessed before (T0) and after (T5) a school-based PA intervention. The PA intervention consisted of coordinative exercises and team sports performed for 1 h twice a week. At T0, girls showed lower PAQ-A, PSQI scores, lower volition facilitators (“self-confidence” and “coping with failure”) and higher volition inhibitor (“postponing training”) than boys. Significant correlations were observed for volition factors an IAT in males and volition and PSQI and IAT in females. After the PA intervention (T5), “postponing training” and “self-confidence” factors were reduced compared to T0. Exercise specialists should consider gender differences in volition in exercise factors during a school-based PA to plan and realize PA protocols aiming at maximizing exercise adherence to tackle sedentary behaviors in adolescents.

In this paper we study the gradient’s boundedness of the minima of the functional where $\Omega \subset {\mathbb {R}}^n$, $2< p< n$ and $u: \Omega \rightarrow {\mathbb {R}}^m$. Regularity results are well-known in the literature; however, we hereby employ an alternative technique to prove the boundedness result. The innovative and original aspect of this paper is to employ some new truncation arguments to find new Caccioppoli type inequalities. These inequalities allow for the use of the Moser’s technique in the vectorial case, in order to get the boundedness result.

Anti-idiotype vaccines utilize idiotype-anti-idiotype network theory, eliminating the need for external antigens as vaccine candidates. Especially for dangerous pathogens, they were safer because they did not contact the live pathogenic microorganisms. However, developing anti-idiotype vaccines with traditional monoclonal and polyclonal antibodies is complex and has a high failure rate. We present a novel, universal, simple, low-cost strategy for producing anti-idiotype vaccines with nanobody technology. Using a neutralization antibody against PCV2-Cap, a nanobody (Ab2) was successfully produced and could mimic the neutralizing epitope of PCV2-Cap. The nanobody can induce protective immune responses against PCV2 infection in mice and pigs. It highlighted that the anti-idiotype vaccine using nanobody has a very good application in the future, especially for dangerous pathogens.

This chapter examines the role of espionage in maintaining the privacy of the state’s interests in the eighteenth century, through a careful examination of sources regarding the State of the Presìdi in Italy. During the 1630s, the Republic of Genoa used a network of parallel espionage to obtain information from the State of the Presìdi, where the Spanish authorities threatened their interests. This non-institutional information channel, which acted in the shadows of the Genoese State Inquisitors, expresses the dark sides of the alliance between Genoa and Madrid, offering a contrasting view with the historiographical stereotype of the so-called Hispano-Genoese symbiosis.

This paper offers a survey of the uses of Iasian marbles and their relationships with other stones. In the 6th century, these marbles were used for wall cladding and flooring, with an aim to alternate with or frame lighter marbles, as was the case in Iasos, in the Agora Basilica. Similar combinations were attested in the cladding of synthronoi of the basilicas A and C of Nea Anchialos. In the latter, the sides of the presbytery were framed with red cipollino slabs of different lengths and widths. In the prostoon, verde antico was added to red and white veined marbles. Other examples seem to demonstrate the use of the red cipollino to mark thresholds and passageways, as was the case of the solea of Hagia Sophia and the presbytery of the basilica of St. John in Ephesos. There, the templon stylobate and bases made of Iasian marble supported columns of verde antico and gray marbles; some slabs would have been of the openwork type; thus, the variety of marbles, their textures and workmanship must have contributed to the deep impact of the arrangement.

In 1905, Einstein gave his first derivation of the mass-energy equivalence by studying, in different reference frames, the energy balance of a body emitting electromagnetic radiation and assuming special relativity as a prerequisite. Here, we reassess the logical soundness of Einstein’s approach and the validity of one assumption crucial for his derivation. That assumption has nothing to do with special relativity. If we accept that assumption as valid, the essence of the mass-energy equivalence (but not its exact formula) can be reached without special relativity or any full-fledged physical theory. However, that assumption is unsupported from a physics viewpoint, and with its use, Einstein was begging the question. We also show why a consequence of the widely received interpretation of E = mc^2 (i.e., every kind of energy has a mass) can be problematic.

We report the optimization of a high-throughput, compliant DNA extraction method that uses standard format 96-well plates and a commercial automated DNA purification system (ABI PRISM® 6100 Nucleic Acid PrepStation). The procedure was set up for maize and soybean, the most common GMO crops and the main ingredients of several foodstuffs, and compared with an EU-validated CTAB-based method. Optimization of the DNA extraction was achieved by applying self-prepared buffers (for DNA extraction, binding, and washing) on the PrepStation loaded with proprietary glass-fiber-coated purification plates. Quantification of extracted DNA was performed by real-time PCR using previously reported endogenous soybean lectin and maize starch synthase genes and a novel plant-specific universal TaqMan MGB probe that targets the 18S rRNA multiple copy gene. Using serial dilutions of both maize and soybean genomic DNAs, we show low PCR sensitivity and efficiency for the official TransPrep DNA extraction protocol compared to the CTAB-based one. On the other hand, using serial dilutions of a standard reference plasmid containing a 137 bp sequence cloned from the 18S rRNA plant-specific ribosomal gene, we demonstrate the high PCR sensitivity and efficiency of the optimized DNA extraction protocol setup with self-prepared buffers. The limits of detection and quantification of the 18S rDNA reiteration were consistent with the calculated values, supporting the suitability of the DNA extraction procedure for high-throughput analyses of large populations and small amounts of tissue.

Background: The role of Pre-participation screening (PPS) modalities in preventing sudden cardiac death (SCD) in athletes is debated due to a high false positive rate. Focused Cardiac Ultrasound (FoCUS) has shown higher sensitivity and specificity, but its cost-effectiveness remains uncertain. This study aimed to determine the diagnostic performance and cost-effectiveness of FoCUS use in PPS. Methods: 2111 athletes (77.4% male, mean age 24.9±15,2years) underwent standardized family and medical history collection (MH), physical examination (PEX), resting ECG, FoCUS (10min/5views-protocol), comprehensive Echocardiography and exercise stress test(EST). We prospectively evaluated three PPS incremental models: Model-A = standardized MH and physical examination; Model-B = Model-A plus resting and stress-ECG; Model-C = Model-B plus FoCUS (10min/5views-protocol). We determined their incremental diagnostic accuracy and cost-effectiveness ratio. Results: 30 athletes were diagnosed with a cardiac condition associated with SCD: 3 were identified by Model-A, 14 by Model-B, 13 athletes by Model-C. The introduction of FoCUS markedly increased the Sensitivity of PPS, compared with model-A and model-B (Sensitivity 94% vs. 19% vs. 58%, Specificity 93% vs. 93% vs. 92%). The total screening cost (TSC) were: Model-A 35.64Eur, Model-B 87.68Eur and Model-C 120.89Eur. Considering the sole conditions at risk of SCD, the Incremental Cost Effectiveness Ratio was 135.62Eur for model-B and 114.31 for model-C. Conclusions: The implementation of FoCUS into the PPS allows to identify a significantly greater number of athletes at risk of SCD and markedly lowers the false negative rate. Furthermore, the incorporation of FoCUS into the screening process has shown to be cost-effective.

Air pollution and the increasing production of greenhouse gases has prompted greater use of renewable energy sources; the EU has set a target that the use of green energy should be at 32 percent by 2030. With this in mind, in the last 10 years, the demand for pellets in Italy has more than doubled, making Italy the second largest consumer in Europe. The quality of the pellets burned in stoves is crucial to indoor and outdoor pollution. Among other parameters, moisture and ash are used to classify pellets according to EN ISO 17225:2014. This work involved the analysis of the organic and inorganic fraction of both some finished products on the Italian market and some raw materials (e.g., wood chips) sampled according to the technical standard EN 14778:2011. The analytical results showed the presence of some substances potentially harmful to human health such as formaldehyde, acetone, toluene and styrene for the organic fraction and nickel, lead and vanadium for the inorganic fraction. The chemometric approach showed that it is the inorganic fraction which is most responsible for the diversification of the samples under study. The detection of some substances may be a warning bell about the impact of such materials, both for the environment and for human health.

In sport, where high achievements are at stake, athletes often feel pressure and emotions that hinder their performance. Emotion regulation becomes essential for athletes to handle stress, achieve optimal performance, and enhance their overall well-being. To advance both research and practical applications, it is crucial to examine the antecedents of emotion regulation and the impact on emotions and other feelings associated with performance. Specifically, the purpose of this cross-sectional study was to examine the role of athletes’ emotion regulation strategies (i.e., cognitive reappraisal and expressive suppression) in the relationship between basic psychological needs satisfaction, emotions, and psychobiosocial experiences. The sample consisted of 424 competitive athletes (246 men and 178 women) involved in individual sports ( n = 164; e.g., fencing, gymnastics, martial arts, swimming, and tennis) or team sports ( n = 260; e.g., basketball, rugby, soccer, and volleyball), aged 16–36 years ( M = 23.08, SD = 7.65). Their competitive experience ranged from 1 to 21 years ( M = 9.71, SD = 6.34) at regional (71%), national (18%), or international (11%) level, and they practiced their sport on average 3.74 times a week ( SD = 1.73). Participants completed measures of basic needs satisfaction (i.e., competence, autonomy, and relatedness), emotion regulation style, emotions, and psychobiosocial experiences. Structural equation modeling results showed that competence need satisfaction was positively associated with pleasant emotions and psychobiosocial experiences that are perceived as functional for performance, and negatively associated with a maladaptive emotion regulation style (i.e., expressive suppression) and unpleasant emotions. Relatedness need satisfaction was positively related to an adaptive emotion regulation style (i.e., cognitive reappraisal), pleasant emotions, and psychobiosocial experiences, and negatively related to expressive suppression and unpleasant emotions. Finally, mediation analysis showed positive indirect effects from autonomy and relatedness satisfaction to pleasant emotions and psychobiosocial experiences via cognitive reappraisal. Findings suggest that the satisfaction of athletes’ basic psychological needs of autonomy and relatedness is related to the experience of pleasant emotions and functional psychobiosocial states when they adopt an adaptive emotion regulation style.

Background Emotions act as mediators of the relationship between stereotypes and inclinations to discriminate against and isolate individuals with substance use disorders (SUD). Emotional responses toward people with SUD are more negative than toward those people with non-drug-related mental disorders. This study explored the effects of affective bonds with substance users and treatment on the type and frequency of emotions, valence, and interpersonal distance. Methods A convenience sample of 1,195 individuals was included in this survey-based study. Participants responded to questions regarding their knowledge of psychoactive drugs and beliefs about substance use disorders and were requested to report the emotions they imagined having felt in four scenarios depicting a substance user whose characteristics varied according to two dimensions: the substance user was a relative or an unknown; the substance user was in treatment for SUD, or not. Results Emotions toward relative drug users were more negative and expressed greater interpersonal distance. Treatment was associated with more positive valence and lower interpersonal distance, but emotions toward relatives in treatment were more negative than those not. Conclusion Specific interventions for relatives of people with SUD may be necessary because of the emotional burden caused by the courtesy stigma.

In this paper we study the regularity of the local minima of the following integral functional $\begin{aligned} J\left( u,\Omega \right) =\int \limits _{\Omega }\sum \limits _{\alpha =1}^{n}\left| \nabla u^{\alpha }\left( x\right) \right| ^{p}+G\left( x,u\left( x\right) ,\nabla u\left( x\right) \right) \,dx \end{aligned}$ (0.1) where $\Omega$ is a open subset of ${\mathbb {R}} ^{n}$ and $u\in W^{1,p}\left( \Omega , {\mathbb {R}} ^{m}\right)$ with $n\ge 2$, $m\ge 1$ and $1<p<n$. In particular, not convexity (quasi-convexity, policonvexity or rank one convexity) hypothesis will be made on the density G, neither structure hypothesis nor radial nor diagonal.

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In recent decades, male infertility has been correlated with the shortening of sperm telomeres. Telomeres regulate the reproductive lifespan by mediating the synapsis and homologous recombination of chromosomes during gametogenesis. They are composed of thousands of hexanucleotide DNA repeats (TTAGGG) that are coupled to specialized shelterin complex proteins and non-coding RNAs. Telomerase activity in male germ cells ensures that the telomere length is maintained at maximum levels during spermatogenesis, despite telomere shortening due to DNA replication or other genotoxic factors such as environmental pollutants. An emerging body of evidence has associated an exposure to pollutants with male infertility. Although telomeric DNA may be one of the important targets of environmental pollutants, only a few authors have considered it as a conventional parameter for sperm function. The aim of this review is to provide comprehensive and up-to-date data on the research carried out so far on the structure/function of telomeres in spermatogenesis and the influence of environmental pollutants on their functionality. The link between pollutant-induced oxidative stress and telomere length in germ cells is discussed.

The incidence of obesity and overweight in the world has been increasing in recent years due to poor diet and lack of physical activity; people suffering obesity and overweight, related with malnutrition due to excess, often resort to calorie restriction diets that are usually not very effective. In this context, intermittent fasting (IF) has become popular due to the possibilities for weight loss that it offers. This diet consists of alternating periods of fasting with unrestricted eating; however, its effectiveness and consequences are unknown to most users. This narrative review analyzes whether intermittent fasting contributes to the improvement of body and metabolic composition. The purpose of the review was to examine the available data on the contribution of intermittent fasting to the improvement of body and metabolic composition, in order to provide information and to define the parameters that condition safe achievement of its benefits. IF dieting triggers adaptive cell responses that cause a decrease in lipid oxidative stress markers in individuals with obesity and prediabetes. Metabolic alterations have been found to go hand in hand with the alteration of circadian rhythms; if IF contributes to this effect, it may assist in treating and preventing obesity and associated diseases. However, there are also disadvantages, such as the loss of lean muscle mass by wasting, and increased hypoglycemia.