Recent publications
While habitat loss and hunting remain the major drivers of biodiversity declines, sublethal disturbances, such as human presence, recreation, and noise also impact wildlife. In response, wildlife often adjust their spatiotemporal behaviors. This study assesses how terrestrial wildlife responds to sublethal disturbances associated with sustainable logging activities. Using camera traps, we conducted a 2‐year continuous survey of two logging compartments within an active, sustainably logged forest reserve in central Sabah, Malaysian Borneo. The survey encompassed periods before, during, and after logging activities, and we obtained daily felling data. Using generalized linear mixed models, we assessed the impact of logging on the spatiotemporal activity of 10 terrestrial mammal and galliform species, considering spatial factors like logging intensity and distance from logging activity, and temporal factors such as days since logging. Four species had a significant and strong response to logging intensity, with varied directions of effect. Only for one species, the response to logging intensity was mediated by time since logging. Though only descriptive, we found no evidence of diel activity shifts, and, for all other species detected during our survey, general patterns in the number of records across each logging period were mixed. Our results highlight the variable and species‐specific responses to logging activity. At a local scale, many species exhibit resilience in their spatiotemporal activity patterns, but for affected species, the effects of logging appear to linger over time. The overall limited response could be due to the relatively low disturbance nature of reduced‐impact logging.
Background
Echolocating bats face an intense arms race with insect prey that can detect bat calls and initiate evasive maneuvers. Their high closing speeds and short biosonar ranges leave bats with only a few 100 ms between detection and capture, suggesting a reactive sensory-motor operation that might preclude tracking of escaping prey. Here we test this hypothesis using greater mouse-eared bats (Myotis myotis) as a model species. With high-resolution biologging tags, we recorded bats hunting aerial prey in the wild and we also collected data from trained conspecifics in the laboratory facing simulated prey escapes of various speeds and distances.
Results
We show that wild bats employed flexible buzz durations during hunting. In the laboratory, such dynamic vocal responses were driven by moving targets, where faster and longer movements led to longer buzzes. During these buzzes, the bats engaged in acute vocal-motor tracking via increased call intervals within 240 ms of evasive prey maneuvers.
Conclusions
Echolocating bats can track evasive prey via a fast vocal-motor feedback loop allowing them to expand their acoustic depth of field. This echo-guided sensory adjustment contributes to the hunting superiority of bats as the most formidable insectivorous predator of the night skies.
The microbial composition of host-associated microbiomes is influenced by co-evolutionary interactions, host genetics, domestication, and the environment. This study investigates the contribution of environmental microbiota from freshwater bodies to the gastrointestinal microbiomes of wild khulans (Equus hemionus hemionus, n = 21) and compares them with those of captive khulans (n = 12) and other equids—Przewalski’s horse (n = 82) and domestic horse (n = 26). Using PacBio technology and the LotuS pipeline for 16S rRNA gene sequencing, we analyze microbial diversity and conduct differential abundance, alpha, and beta diversity analyses. Results indicate limited microbial sharing between wild khulans and their waterhole environments, suggesting minimal environmental influence on their gut microbiomes and low levels of water contamination by khulans. Wild khulans exhibit greater microbial diversity and richness compared to captive ones, likely due to adaptations to the harsh nutritional conditions of the Gobi desert. Conversely, captive khulans show reduced microbial diversity, potentially affected by dietary changes during captivity. These findings highlight the significant impact of environment and lifestyle on the gut microbiomes of equids.
Understanding biodiversity patterns is essential for ecology and conservation. Globally, conservation efforts often prioritize tropical rainforests due to their high species richness. At the regional scale, the same is true in the Greater Annamites ecoregion of Vietnam and Laos, where conservation efforts have largely focused on broadleaf wet evergreen forest, despite the fact that other habitats remain threatened. One such habitat is the coastal dry forest landscape in southern Vietnam, which has received little conservation focus despite the fact that its forested areas have been severely reduced. Nui Chua National Park (NP) in southern Vietnam harbors one of the few remaining sizable areas of dry coastal forest. In this study, we used camera‐trap data and a community Royle‐Nichols model to explore community structure of ground‐dwelling mammal and birds along a complex habitat gradient in Nui Chua NP. We first investigated species associations among three habitat types: dry forest, semi‐dry forest, and broadleaf wet evergreen forest. We then used occupancy‐based diversity profiles to assess diversity in these three habitats. Overall species diversity tended to be highest in the transitional semi‐dry forest ecotone, which supported species from both dry and evergreen forests. Notably, the semi‐dry forest also had the highest occupancies for several endemic and threatened species. Our findings highlight the importance of the semi‐dry forest for conservation in the broader coastal dry forest landscape. We emphasize the need for fine‐scale biodiversity assessments to inform conservation strategies, especially in habitats that may be overlooked by broader‐scale conservation strategies.
Laypeople often struggle to understand the provisional nature of scientific knowledge. While scientific knowledge may be widely accepted within the scientific community, it is continually subject to revision and further development as new studies are published. These characteristics of science, where findings build upon each other over time rather than being entirely replaced by new discoveries, are not always well understood by the public. This becomes particularly problematic when research process presentations that emphasize the evolving, provisional nature of scientific knowledge are perceived as less credible, reinforcing misconceptions about the integrity and nature of science. In two experimental online studies on science education ( n 1 = 99; n 2 = 184), we examined how different representations of the scientific process affect perceptions of credibility and tentativeness using text- and video-based presentations in the context of bat ecology as an example. In both studies, we varied the presentation of scientific practices (without explanations vs. with explanations) and the portrayal of the scientist’s deliberations (canonized vs. authentic). Our findings indicate that, although scientific knowledge is perceived as provisional, the way it is communicated can influence its perceived credibility. In both studies, perceived tentativeness was negatively correlated with perceived credibility, highlighting a challenge in science communication: the need to convey the evolving nature of scientific knowledge without undermining trust in its reliability.
As obligate nocturnal mammals, most bats spend the day hiding in dark shelters, sometimes as gregarious colonies. Roosting in such large colonies may have the advantage of reducing thermoregulatory costs, as the heat dissipated by thousands of bodies raises the temperature of the cave interior. However, bats in large aggregations may also suffer from increased predation risk. Here we studied the effect of a highly gregarious Palaeotropical bat, the wrinkle-lipped free-tailed bat (Mops plicatus), on cave temperature and the factors that influence its emergence behaviour. We studied cave temperature, prey abundance, emergence behaviour and attack rates by birds of prey during colony emergence. The study was made at Khao Wongkot Cave, counting more than one million individuals. In all seasons, we observed that the presence of M. plicatus caused relatively higher temperatures of around 30°C inside the cave, while temperatures outside were lower and more variable. This suggests that the body heat of aggregated bats raised the temperature inside the cave, presumably close to the thermo-neutral zone at the roosting sites of bats. Arthropod abundance was highest during late pregnancy/early lactation and lowest during late lactation/post lactation. Bats emerged earliest during the late wet season and latest during the hot season. During periods of high predation risk, bats always emerged as a column, whereas during periods of low predation risk (hot and early wet seasons), individual bats left first, before a column of bats formed, 10 to 20 min after the first individuals exited the cave. Our study highlights that large colonies may provide thermoregulatory benefits that could increase individual fitness. However, aggregations also increase predation costs during emergence. Bats form a column when aerial predator attacks are most frequent. Also, bats seem to adjust their emergence timing in response to seasonal changes in predation risk.
Survival and cause‐specific mortality rates are vital for evidence‐based population forecasting and conservation, particularly for large carnivores, whose populations are often vulnerable to human‐caused mortalities. It is therefore important to know the relationship between anthropogenic and natural mortality causes to evaluate whether they are additive or compensatory. Further, the relation between survival and environmental covariates could reveal whether specific landscape characteristics influence demographic performance. We used telemetry data on 681 Eurasian lynx (Lynx lynx), a model apex predator with large spatial requirements, that were tracked across their European distribution. Through time‐to‐event analyses, we sought to determine the variables associated with differences in their survival. Illegal killing was the main cause of mortality (33.8%), and mortality rates were similar in protected and hunted populations (8.6% and 7.0% per year, respectively). Survival varied greatly across populations (70–95% per year). Across all study sites, higher hunting and anthropogenic mortality rates were partially compensated by lower rates of other mortality causes but not by natural mortality alone. Variation in survival depended on sex (female survival was 1.5 times greater than male survival) and seasonality (highest risk during hunting season and winter), and lower survival rates were correlated with higher human modification of landscapes at both coarse (home range composition) and fine (habitat use within home range) scales. Some variation in survival was driven by unobserved factors, which, given the high rates of human‐caused mortalities, including illegal killing, are of foremost concern. Due to the low natural mortality rates in protected and hunted populations, we conclude that anthropogenic causes of mortality are likely close to additive, such that maintaining or increasing refuge habitat with little human disturbance is critical to lynx conservation.
Urbanization as a major driver of global change modifies biodiversity patterns and the abundance and interactions among species or functional species groups. For example, urbanization can negatively impact both predator–prey and mutualistic relationships. However, empirical studies on how urbanization modifies biotic, particularly multitrophic, interactions are still limited. In this study, we applied a framework focused on a predator–prey–mutualistic relationship involving communities of insect‐pollinated vascular plants, pollinators (bees and hoverflies), predatory spiders, and sand lizards as top predators to test (i) the effect of urbanization on abundance and species richness at different trophic levels and (ii) the effect of urbanization on the regulation of biotic interactions using correlations between species abundances as a proxy. By assessing 56 dry grassland patches in Berlin, Germany, we found that higher trophic levels (sand lizard abundance as well as predatory spider species richness and abundance) were significantly impacted by urbanization whereas pollinators were affected to a lesser degree (only abundance, but not species richness). In contrast, insect‐pollinated vascular plants were not impacted by urbanization. Path analyses revealed significant relationships in low‐urbanized areas. In these areas, we observed significant bottom‐up‐regulated mutualistic and predator–prey interactions (plants–pollinators, and pollinators–predatory spiders), as well as top‐down‐regulated predator–prey interactions (sand lizards–pollinators, and predatory spiders–pollinators). In contrast, no significant interactions were found in highly urbanized sites. Our results suggest that bottom‐up regulation is stronger than top‐down regulation in low‐urbanized areas. To our knowledge, this is the first study to examine the effects of urbanization on predator–prey–mutualistic interactions and to determine whether these interactions are regulated by bottom‐up or top‐down processes. These findings enhance our understanding of multitrophic interactions in urban environments and their associated ecosystem services, such as pollination, thereby supporting efforts in urban biodiversity conservation.
Peccaries (collared peccary—CP—and white-lipped peccary—WLP) are an essential source of protein and income for rural communities in the Amazon region. Since 1980s, researchers in the Amazon have reported recurrent local disappearances of WLP populations. Although such disappearances impact the species conservation and the food security of rural societies, no studies have drawn consistent conclusions about the causes of these population collapses. However, it has recently been proposed that the overabundance of this species before its decline would be related to infectious disease outbreaks. In the current study, we aimed to determine the circulation (occurrence and exposure) of viruses relevant to swine health in CP and WLP populations, namely classical swine fever virus (CSFV), Aujeszky's disease virus (ADV), swine vesicular disease virus (SVDV), and porcine circoviruses (PCV). The study was conducted in two areas of the northeastern Peruvian Amazon: the Yavarí-Mirín River basin (2008 -2020), where WLPs experienced extreme population fluctuations, and the Pucacuro National Reserve (2012–2014), where no WLP disappearances have been reported. Since WLP is not easily found during population declines, we also sampled CP as an indicator of virus circulation in the area as they are likely to be susceptible to the same pathogens. CSFV and ADV antibodies were detected in both peccary species and both areas. Diseases caused by CSFV and ADV have the potential to act as ultimate causes of population collapse, especially in large WLP populations where overabundance could increase the rate of pathogen transmission. Our results were inconclusive in establishing whether or not these viruses drove the WLP population to collapse, but their potential role warrants deeper investigation, expanding the geographical coverage of studies on infectious diseases in peccaries.
Wind turbines used to combat climate change pose a green-green dilemma when endangered and protected wildlife species are killed by collisions with rotating blades. Here, we investigated the geographic origin of bats killed by wind turbines along an east-west transect in France to determine the spatial extent of this conflict in Western Europe. We analysed stable hydrogen isotopes in the fur keratin of 60 common noctule bats (Nyctalus noctula) killed by wind turbines during summer migration in four regions of France to predict their geographic origin using models based on precipitation isoscapes. We first separated migratory from regional individuals based on fur isotope ratios of local bats. Across all regions, 71.7% of common noctules killed by turbines were of regional and 28.3% of distant origin, the latter being predominantly females from northeastern Europe. We observed a higher proportion of migratory individuals from western sites compared to eastern sites. Our study suggests that wind-turbine-related losses of common noctule bats may impact distant breeding populations across whole Europe, confirming that migratory bats are highly vulnerable to wind turbines and that effective conservation measures, such as temporary curtailment of turbine operation, should be mandatory to protect them from colliding with the rotating blades of wind turbines.
Genomics is an invaluable tool for conservation, particularly for endangered species impacted by wildlife trafficking. This study uses genomic data to provide new insights to aid conservation and management of endangered species, using as a case study the Yellow cardinal (Gubernatrix cristata), a bird endemic to southern South America severely affected by illegal trade and the transformation of its natural habitat. We explore population structure within the Yellow cardinal, delimiting management units and describing connectivity among them. Additionally, we develop and assess the accuracy of a panel of 189 informative SNPs, and demonstrate how these can reliably assign confiscated individuals to one of the management units established. Lastly, we assess hybridization between the Yellow cardinal and the Diuca finch (Diuca diuca), which is reported to occur in regions of sympatry. We confirm that hybridization occurs, although it is not as common as previously thought, and that hybrids might be fertile, as we found evidence of backcrossing with Yellow cardinals. We discuss the implications of this introgression for the evolution and conservation of Yellow cardinals. Our study provides new, valuable information that can guide conservation efforts, comprising a test case for the use of genomics in combating illegal trafficking, with potential application beyond the case of the Yellow cardinal.
Freshwater ecosystems are highly biodiverse¹ and important for livelihoods and economic development², but are under substantial stress³. To date, comprehensive global assessments of extinction risk have not included any speciose groups primarily living in freshwaters. Consequently, data from predominantly terrestrial tetrapods4,5 are used to guide environmental policy⁶ and conservation prioritization⁷, whereas recent proposals for target setting in freshwaters use abiotic factors8, 9, 10, 11, 12–13. However, there is evidence14, 15, 16–17 that such data are insufficient to represent the needs of freshwater species and achieve biodiversity goals18,19. Here we present the results of a multi-taxon global freshwater fauna assessment for The IUCN Red List of Threatened Species covering 23,496 decapod crustaceans, fishes and odonates, finding that one-quarter are threatened with extinction. Prevalent threats include pollution, dams and water extraction, agriculture and invasive species, with overharvesting also driving extinctions. We also examined the degree of surrogacy of both threatened tetrapods and freshwater abiotic factors (water stress and nitrogen) for threatened freshwater species. Threatened tetrapods are good surrogates when prioritizing sites to maximize rarity-weighted richness, but poorer when prioritizing based on the most range-restricted species. However, they are much better surrogates than abiotic factors, which perform worse than random. Thus, although global priority regions identified for tetrapod conservation are broadly reflective of those for freshwater faunas, given differences in key threats and habitats, meeting the needs of tetrapods cannot be assumed sufficient to conserve freshwater species at local scales.
Ensuring animal welfare is a key aspect of animal management in zoological facilities and aquaria, representing a pivotal facet of their mission. Italy currently lacks a comprehensive and valuable assessment methodology for evaluating the welfare of captive animals. To address this gap, the present study aimed to identify the most important criteria that should be considered in the welfare management and assessment of animals housed in Italian zoos and aquaria. To pinpoint this issue, we engaged experts with diverse backgrounds, structuring their communication throughout an iterative process, by applying the Delphi methodology. A pilot and three Delphi rounds were administered online to 74 experts, asking them to a) validate relevant topics derived from current legislation and guidelines divided into three clusters: Care, Wellbeing, and Regulation; b) assess the relevance of these topics across taxonomic groups; c) propose and confirm indicators for each identified topic; d) suggest and refine a list of questions for animal welfare assessment. The results were three lists of questions, one for each cluster, with a total of 80 topics, 174 indicators, and 272 questions identified by the experts. The aspects included in these lists offer valuable insights into the main aspects experts consider relevant for captive animal welfare. Despite the complexity of animal welfare and the huge amount of species hosted in zoos limiting the possibility to cover this aspect with a single expert consultation, this project actively addresses the urgent need for standardization in animal welfare assessment, contributing to the ongoing development of zoological regulations in Italy. This is especially important given the current limited legislative framework, underscoring the link between animal welfare and successful ex-situ species conservation. These questions can be the basis of fine-tuned protocols to be tested in future projects aiming at animal welfare self-assessment, thus supporting authority inspection processes.
Successful invasive species increase their spreading success by trading-off nutritional and metabolic resources allocated to reproduction and range expansion with other costly body functions. One proposed mechanism for the reallocation of resources is a trade-off with the immune function and the regulation of oxidative status. Relying on a panel of blood-based markers of immune function and oxidative status quantified in an invasive species (Egyptian goose) and two native competing species (mallard and mute swan) in Germany, we tested the hypothesis that the invasive species would have (i) lower investment in immune function, (ii) lower levels of oxidative damage, and (iii) no higher antioxidant defences compared to the native species. We found lower levels of adaptive immune markers (lymphocytes and immunoglobulin Y), in the invasive species compared to the two native species. Innate immune profile was generally similar between Egyptian geese and mallards. By contrast, mute swans showed higher levels of heterophils and lysozymes, and lower levels of bacteria killing ability compared to both Egyptian geese and mallards. Mute swans also showed higher levels of haemolysis and haemagglutination, but lower levels of monocytes and haematocrit compared to Egyptian geese. Reactive oxygen metabolites, a marker of oxidative damage, were higher in mallards and lower in Egyptian geese compared to the other waterfowl species, while levels of antioxidants were generally similar among the three species. Our results point to a reduced investment in adaptive immune function in the invasive species as a possible resources-saving immunological strategy due to the loss of co-evolved parasites in the new colonised habitats, as observed in a previous study. A lower investment in immune function may benefit other energy-demanding activities, such as reproduction, dispersal, and territoriality, while maintaining relatively higher innate immunity is beneficial since invasive species mainly encounter novel pathogens. Results pointed out also other important species-specific differences in baseline immune status, supporting previous findings on the relationship between species' body mass and immune profile.
Our study addresses the urgent need to identify and prioritise threatened species in Vietnam for conservation and recovery. With a focus on Vulnerable, Endangered, and Critically Endangered species with high levels of endemicity (> 50% of their global range occurs in Vietnam), we conducted a comprehensive analysis of key species for conservation prioritisation, IUCN Green Status Assessment, and recovery strategies within the Vietnamese landscape. By integrating data on provincial hotspots and protected areas as key conservation sites, we established a robust framework to assess key species richness, threats, and conservation needs. We identified 203 key species across 12 taxonomic classes, establishing a viable list of wildlife recovery priorities. Habitat loss and exploitation were identified as primary threats to key species; alarmingly, only 38% of these key species had > 50% of their range within protected areas, emphasizing urgent conservation needs that extend beyond current protected area boundaries. Our findings hold significance for guiding conservation strategies, resource allocation, and policy decisions in relation to Vietnam's National Biodiversity Strategy and Action Plan (NBSAP) and inform measurable species prioritisation goals under Target 4 of the Convention on Biological Diversity (CBD). By pinpointing species and regions that require immediate attention, our study provides essential guidance for policymakers, conservationists, and resource managers/donors. Acting on these insights will enhance the effectiveness of conservation efforts and ensure the long-term sustainability of wildlife as a vital natural heritage.
While the production of a draft genome has become more accessible due to long-read sequencing, the annotation of these new genomes has not been developed at the same pace. Long-read RNA sequencing (lrRNA-seq) offers a promising solution for enhancing gene annotation. In this study, we explore how sequencing platforms, Oxford Nanopore R9.4.1 chemistry or PacBio Sequel II CCS, and data processing methods influence evidence-driven genome annotation using long reads. Incorporating PacBio transcripts into our annotation pipeline significantly outperformed traditional methods, such as ab initio predictions and short-read-based annotations. We applied this strategy to a nonmodel species, the Florida manatee, and compared our results to existing short-read-based annotation. At the loci level, both annotations were highly concordant, with 90% agreement. However, at the transcript level, the agreement was only 35%. We identified 4,906 novel loci, represented by 5,707 isoforms, with 64% of these isoforms matching known sequences in other mammalian species. Overall, our findings underscore the importance of using high-quality curated transcript models in combination with ab initio methods for effective genome annotation.
The diet of Western human societies is characterized by an excess of saturated fatty acids (FAs) and a high concentration of ω-6 relative to ω-3 polyunsaturated FA (PUFA). These unbalanced diets are suspected to trigger diseases and disorders. To alleviate this public health concern, the production of healthier meat with more PUFAs of higher ω-3 concentration could potentially be achieved by modifying livestock diets. The high nutritional value and limited breeding costs of edible insects have brought insect feed into the discussion as a promising fat source for animal and human diets. In this study, we sought to increase the amount of ω-3 PUFAs in mealworm (Tenebrio molitor) larvae. We investigated the effects of diets varying in ω-3/ω-6 ratio and FA concentration but similar in PUFA proportion on larva FA composition. Mealworm larvae showed significant plasticity in lipid composition. High dietary ω-3/ω-6 ratios induced an increase in the proportion of ω-3 and a decrease in ω-6, which resulted in higher larval ω-3/ω-6 ratios, but also in higher larval PUFA proportion. Increasing FA concentrations in larva diets also favored the accumulation of PUFAs to the detriment of monounsaturated and saturated FAs. Providing ω-3-rich seeds to mealworm larvae could allow the production of economical animal fat with healthier PUFA percentages (> 60%) and ω-3/ω-6 ratios (>0.5).
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