Kazan Federal University

The bacteriophage Pseudomonas phage Ka1 was isolated from the water of a tributary of Lake Baikal, in the area of discharge of treated wastewater in the city of Slyudyanka. The bacteriophage genome is 46,092 bp in size. and 50% composition of G + C pairs (AN OP455935.1) has 91% identity with the genome of Pseudomonas phage PSA37, belonging to the Bruynoghevirus from the class Caudoviricetes, which characterizes it as a new representative of Luz24-like phages (Bruynoghevirus). The bacteriophage lyses 62% of clinical isolates of Pseudomonas aeruginosa and is able to increase the effectiveness of gentamicin, ciprofloxacin, imipinem and meropenem by 4–8 times against this bacterium. Integrases, transposases and recombinases are not identified in the genome, which makes Pseudomonas phage Ka1 possible for use in complex therapy of infections caused by P. aeruginosa.

Chelating agents used in agriculture and land reclamation practices must exhibit the capacity to firmly bind metal ions while also rapidly decomposing into non-toxic compounds due to their introduction into the environment in substantial quantities. It is therefore crucial to identify an alternative to EDTA, a prevalent chelating agent known for its low biodegradability and relatively high toxicity. This study focuses on assessing the degree of biodegradation of iminoglutamic succinic acid (IGSA) and the toxicity of its half-decomposition products. For this purpose, two bacterial and fungal isolates capable of decomposing IGSA were isolated and characterized, identified as Ralstonia pickettii and Fusarium foetens, respectively. The results of a 28-day experiment demonstrated that Ralstonia pickettii was capable of decomposing IGSA by 18.0% according to the manometric test and by 24.5% based on the analysis of decomposition products via HPLC. In comparison, Fusarium foetens exhibited a more effective biodegradation capacity, with rates of 20.3% and 32.5%, respectively. The half-decomposition products of IGSA were characterized by low ecotoxicity levels (LID10) concerning Paramecium caudatum (11.2–13.2 g/l) and Ceriodaphnia affinis (3.6–8.9 g/l). However, a stimulating effect was observed on microalgae, with growth stimulation ranging from 63.65–96.60%.

The study revealed the enhanced conformational flexibility of thiacalix[4]arene Schiff base derivatives, which drastically affected their coordination and H-bonding behavior.

The superconducting state in heterostructures containing layers of a superconductor and a ferromagnet, which is split into domains, has been studied taking into account the long-range electron–electron interaction in the ferromagnetic layer. The critical temperature and spatial distribution of the order parameter in different heterostructures have been calculated. Various types of spin valves have been examined, including a valve based on solitary reentrant superconductivity in asymmetric superconductor/superconductor/superconductor systems. A shift of the minimum has been observed in the dependence of the critical temperature of the superconductor/superconductor/superconductor system on the thickness of the ferromagnetic layer due to the presence of a domain structure. A state with alternating signs of the phase of the superconducting order parameter in one of the superconducting layers against the background of domain walls has been obtained. In superconductor/superconductor/superconductor systems, the alternation of the phase sign is also possible at the edge of one of the ferromagnetic layers because of the alternation of the magnetization direction. The possibility of using the sensitivity of the order parameter phase to magnetic inhomogeneities to create a controllable 0–π Josephson junction is discussed.

The implementation of decarbonization strategies requires more active involvement of industrial corporations. Their motivation is rather low because they are not able to incorporate their profitability goals in the decarbonization process. The literature on orchestrating the corporate performance and decarbonization goals is limited. The purpose of this study is to develop theoretical and methodological foundations of the decarbonization management mechanism allowing for mutual coordination of corporate performance and decarbonization goals through adjusting corporate indicators and low-carbon targets. The concept of decoupling of corporate indicators and the concept of green orchestration have been the theoretical foundations of the study. The empirical results have validated the methodology used to develop a decarbonization management mechanism.

Four novel HLA alleles HLA‐A*02:01:222, ‐B*07:02:104, ‐C*07:02:156 and ‐DQB1*06:03:51 alleles detected during routine next generation sequencing.

Two novel HLA‐С alleles HLA‐C*07:1153 and ‐C*07:1154 alleles detected during routine next generation sequencing.

The review is devoted to analysis of recent advances, problems, and practical applications of enzymes in the framework of chemical enzymology and enzyme engineering. The achievements in the fundamental understanding of the molecular mechanisms of the catalytic cycle of enzymatic reactions are considered using quantum/molecular mechanics methods with supercomputer technologies and bioinformatics methods. The construction of protein biocatalysts with new properties is a fundamentally significant methodology of the bioengineering approach to solving practical problems, which is demonstrated by a number of examples. The increasing role of biocatalysis in medicine and biomedical research is illustrated by solving the problems of antibiotic synthesis and overcoming bacterial resistance to them, studying the mechanisms of neurodegenerative diseases and developing drugs to treat Alzheimer's disease, and investigation of biocatalytic processes of DNA repair and the role of mechanisms of functioning of heme peroxidases in the human body. The use of enzymes that destroy various endogenous and exogenous toxicants has been greatly developed in recent decades. The problems of using enzymes in therapy and drug delivery are analyzed. The fundamental role of enzymes in modern analysis and diagnosis is noted. The review considers a new trend in the development of bioanalytical methods using aptamers, multianalytical biochips, surface-enhanced Raman scattering systems, and bioelectroanalysis. The bibliography includes 460 references.

The presented article develops the thesis that Muslim women in Kazan act as actors who, in accordance with their needs, seek to change the structural conditions of their lives. In particular, it is about getting new positions, guaranteeing resources in the form of new knowledge, education and social stability. Positioning yourself as a moral subject allows you to get a new authoritative role, which makes it possible to advance your understanding of many Muslim and national traditions and symbols. In the context of modern society, Muslim women of Kazan emphasize the importance of education as a tool for emancipation. They strive to obtain higher education not only for personal development, but also to create a new social status. This desire to go beyond traditional roles is a manifestation of their desire for social justice and equality. Muslim women transmit their understanding of such important issues as women's Muslim clothing and the language of communication to the Islamic community of Tatarstan.

The crystal structure and vibrational spectra of CoFe 2 O 4 ferrite were studied using X-ray diffraction and Raman spectroscopy over a pressure range of 0–35[Formula: see text]GPa. A structural phase transition from the cubic [Formula: see text] phase to the post-spinel orthorhombic Bbmm phase occurs at a pressure of approximately 23[Formula: see text]GPa through a two-phase region. Pressure-induced changes in the structural parameters, lattice distortion, and vibrational modes of the studied ferrite were investigated in detail. Lattice parameters, bond lengths, compressibility, and bulk modulus for both the cubic and orthorhombic phases of CoFe 2 O 4 were determined.

Рассматриваются однопараметрические семейства порождающих квантовых каналов. Такие семейства называются порождающими квантовыми динамическими отображениями или порождающими квантовыми процессами. Под порождающими каналами составных квантовых систем понимаются каналы, по которым однозначно определяются каналы составляющих подсистем, называемые порожденными каналами. Изучаются свойства биективных квантовых каналов, а также свойства квантовых процессов, состоящих из таких каналов, с использованием критерия о порождающем и порожденном линейных отображениях. По порождающему квантовому динамическому отображению строится естественным образом порожденное динамическое отображение. Показано, что свойства непрерывности и вполне положительной делимости порождающих квантовых динамических отображений являются наследственными для порожденных динамических отображений. В качестве приложения полученных результатов построены непрерывные вполне положительные эволюции. Для порождающих квантовых динамических отображений, принимающих значения в множестве каналов, демпфирующих фазы, получен критерий вполне положительной делимости.

Ionic liquids (ILs) are salts which persist in liquid state near room temperature. They are characterized by high thermal and chemical resistance, good solubility, and high ionic conductivity. ILs can be used as permeability enhancers for transdermal delivery of drugs. The study of the interaction of ILs with lipids is important for understanding their potential toxicity to cells and the environment. In this work, we discuss features of the molecular structure and mobility of the aqueous system consisting of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and ethylammonium nitrate ionic liquid (EAN) mixtures studied by NMR and computer simulation MD methods. The ³¹P NMR line shape analysis revealed two lipid states in the systems: in D2O it is a lamellar liquid crystalline state associated with the formed vesicle-like structures of DMPC, while in EAN it is isotopic. The ratio of these states correlates with the ratio of solvents in the system. Based on the self-diffusion coefficients obtained by NMR, sizes of the diffusing particles were estimated. The method of MD showed that DMPC molecules assemble into micelles in the presence of water. In the mixtures of EAN and water the configuration of DMPC molecules changed. When DMPC interacts only with EAN, the micelle disintegrates. It is thus inferred that the presence of IL in the environment significantly affects the structure of the lipid system. The comparative analysis of the SDCs revealed a correlation between values obtained by MD and NMR methods.

It has been shown that human seminal fluid is a major factor in enhancing HIV activity. The SEM2(49–107) peptide is a product of cleavage after ejaculation by internal prostheses of the semenogelin 2 protein, expressed in seminal vesicles. It is established that the peptide SEM2(49–107) forms amyloid fibrils, which increase probability of contracting HIV infection. In this nuclear magnetic resonance (NMR) study, we present almost complete (86%) resonance assignments for the ¹H ¹⁵N and ¹³C atoms of the backbone and side-chain of the SEM2(49–107) peptide (BioMagResBank accession number 52356). The secondary structure of SEM2(49–107) peptide was estimated by using two approaches, secondary chemical shifts analysis (CSI) and TALOS-N prediction. Analysis of the secondary structure of the SEM2(49–107) peptide using both methods revealed that the peptide contains helical segments at the C-terminus. Also in this work, we used phase-sensitive 2D HSQC ¹H- ¹⁵N experiments measuring longitudinal T1 and transverse T2 NMR relaxation times to report predicted secondary structure and backbone dynamics of the SEM2(49–107) peptide. This resonance assignment will form the basis of future NMR research, contributing to a better understanding of the peptide structure and internal dynamics of the molecule.

Subject. The article considers various types of reporting as sources of information on the research activities of higher education institutions in Russia to assess its effectiveness. Objectives. The aim is to examine the current reporting system based on the specifics of the activities of Russian universities in order to identify problematic aspects of the presentation of information on research and development, determine further prospects for improving the structure and content of reporting on the results of research activities of universities. Methods. The study employs general scientific methods of cognition, including analysis, synthesis, observation, systematization, classification, comparison, generalization. Results. We reviewed forms of statistical monitoring of university activities in the field of research and development, the structure and content of reporting, examined the structure and content of university self-examination reports characterizing research activities, investigated possibilities for evaluating the research activities of the report on intellectual capital of universities, integrated reporting, and comprehensive public reporting of universities. Conclusions. All reports are generated with varying level of detail, the same information is duplicated in different forms of reporting, some reports are overloaded. Reporting is mainly aimed at satisfying the interests of government regulators and does not meet the needs of other stakeholders. This necessitates the development of modern advanced reporting concepts, taking into account the specifics of universities to meet the needs of a wide range of users.

Biocontrol strains of Pantoea brenneri expressing tdTomato fluorescent protein were obtained for further study of the bacteria’s ability to colonize plant surfaces. It was found that expression of the tdTomato fluorescent protein gene did not affect biomass accumulation and PGP- properties of the strains: ability to synthesize IAA, siderophores and biofilm production. Maximum production of fluorescent protein by P. brenneri strains was observed at 18 h of cultivation. The data obtained allows further experiments to assess plant colonization by P. brenneri strains.

The level of expression of srfAD gene, a subunit AD of surfactin synthetase, and the yield of the total lipopeptide fraction were studied in relation to the composition of nutrient medium and the growth stage of the probiotic strain Bacillus subtilis GM5. The maximum concentration of the total lipopeptide fraction was observed during the growth of strain GM5 on SMN medium with soybean flour. The relative expression of the srfAD gene of B. subtilis GM5 was quantified by RT- qPCR. We conclude that the increase in the concentration of the total lipopeptide fraction on SMN medium correlates with the expression of the srfAD gene during 96-hour cultivation of GM5, which may indicate the production of mainly lipopeptide from the surfactin group by under these conditions.

Populations of species living at the edge of their range need long-term and constant monitoring to analyze the number and condition of individuals of the species. When populations fall out of natural phytocenoses in conditions of regulated nature management, it is possible to restore these cenopopulations by repopulation. For optimization of work on structuring of data on populations, a GIS-map of medicinal plants was created. A full set of studies has been carried out for Bupleurum aureum populations, which are experiencing significant anthropogenic pressure near the western border of its range. The species has practically disappeared from the broad-leaved forests of the Predvolga region. The created GIS-map contains information with precise georeferencing of the identified cenopopulations in relation to landscape and soil type. Another block of the GIS-map database contains information on productivity and genetic polymorphism of cenopopulations, which should be taken into account when restoring lost populations.The conducted studies have shown that the share of generative plants of B. aureum in the population decreases when the light intensity decreases in the forest. In this case, individuals preserved in meadows adjacent to the forest are of great importance. It can be assumed that forest edge communities are important key sites for the conservation of forest biodiversity.

Creating synthetic datasets for artificial intelligence training has a crucial role in modern developments. Considering the difficulties in collecting real data, which is often a costly and time-consuming process that requires significant resources and time. Synthetic data, on the other hand, allows generating large amounts of varied and controlled data that can be customized for specific training and testing needs. This makes the process of algorithm development and improvement more efficient and affordable. This paper presents a comprehensive tool for creating synthetic motion datasets based on rigging a 3D robot model. The ability to create and edit animations through the Blender interface is described. It supports a variety of well-known 3D model formats, providing flexibility in use, and includes powerful tools to achieve high-quality visual effects and realistic scenes. In addition, the tool can automatically generate a large number of robot images needed for training neural networks. By utilizing these capabilities, the tool greatly simplifies the creation of training datasets, making the process more efficient and affordable. Possible future enhancements include automation of rigging, further optimizing the functionality and usability of the tool for robotics and machine learning.