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    ABSTRACT: In the present paper, we reported the chromosome numbers and karyotypes of five Hieracium species (H.medianiforme (Litw.et Zahn) Juxip, H. karagoellense (Zahn) Sell&West, H. argillaceoides (Litw.et Zahn) Juxip, H. asterodermum (Woronow &Zahn) Juxip, H. umbellatum L.) from Turkey. Among them, three are tetraploid with 36 somatic chromosomes and one triploid with 27 chromosomes, and the other one diploid with 18 chromosomes, respectively. The basic number x = 9 was confirmed for all of them and their karyotypes consist mainly of metacentric and submetacentric chromosomes. Chromosome morphologies of four species were firstly reported.
    No preview · Article · Jan 2014 · Caryologia -Firenze-
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    ABSTRACT: The chitinase B (chiB) and C (chiC) genes and flanking regions from a local isolate of Serratia marcescens were cloned individually and sequenced. Results showed that these chiB and chiC genes have a 96 % maximum similarity with chiB and chiC from different S. marcescens species (GenBank numbers Z36295.1 and AJ630582.1, respectively). The amplified chiB fragment, including some upstream and downstream regions, is 1,689-bp long with an open reading frame of 1,500 bp. The amplified fragment of chiC is 1,844 bp with an open reading frame of 1,443 bp. These sequences were submitted to the GenBank with accession numbers JX847796 (chiB) and JX847797 (chiC). Putative promoter regions and Shine-Dalgarno sequences were identified in both genes. The genes were cloned into a shuttle vector and the constructs were designated as pHYSB and pHYSC, respectively. Both plasmids were introduced separately into kurstaki and israelensis strains of Bacillus thuringiensis and the insecticidal activities of the engineered B. thuringiensis strains were assayed in larvae of Galleria mellonella and adult of Drosophila melanogaster. Engineered B. thuringiensis strains showed higher insecticidal activity than parental strain and the parental S. marcescens. In addition, pHYSB and pHYSC were stable over 16 daily passages under non-selective conditions in transformed B. t. israelensis 5724 strain.
    No preview · Article · Jun 2013 · Current Microbiology
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    ABSTRACT: A novel moderately thermophilic, Gram-positive, endospore-forming, rod-shaped, motile bacteria and alkaline active xylanase producing strain D1021(T) , was isolated from Kaynarca hot spring in the province of Izmir, Turkey and was characterized in order to determine its phylogenetic position. Growth was observed at 35-70 °C (optimum 60 °C), at pH 6.0-10.0 (optimum pH 7.0). The strain D1021(T) grew on a wide range of carbon sources including ribose, xylose, glucose, maltose, sucrose, arabinose, and melibiose. The major isoprenoid quinone was MK-7. 16S rRNA gene sequence analysis showed that strain D1021(T) is related to members of genus Anoxybacillus, with similarities to Anoxybacillus spp. varying from 94.7 to 98.7. The major fatty acids of strain D1021(T) were iso-C(15:0) (57.46%) and iso-C(17:0) (13.98%). The DNA G + C content was 42.9 mol %. DNA-DNA relatedness values between Anoxybacillus spp. and D1021(T) were lower than 70%. On the basis of phenotypic characteristics, rpoB analysis, phylogenetic and DNA-DNA hybridization data, it was proposed that the strain D1021(T) (= DSM 21706(T) = LMG 25303(T) ) should be placed in the genus Anoxybacillus as the type strain of a novel species, Anoxybacillus kaynarcensis sp. nov. The GenBank accession number for the 16S rRNA sequence is EU926955. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
    Full-text · Article · May 2013 · Journal of Basic Microbiology
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