Florida Southern College

Coral reefs and their photosynthetic algae form one of the most ecologically and economically impactful symbioses in the animal kingdom. The stability of this nutritional mutualism and this ecosystem is, however, at risk due to increasing sea surface temperatures that cause corals to expel their symbionts. Symbioses with these microeukaryotes have independently evolved multiple times, and non‐coral cnidarians (e.g., sea anemones) serve as a valuable and insightful comparative system due to their ease of husbandry in the laboratory and their ability to shuffle different strains of their photosymbionts to acclimate to thermal conditions. This breadth of symbiont shuffling is exemplified by the sea anemone Anthopleura elegantissima , which naturally occurs in symbiosis with the dinoflagellate Breviolum muscatinei (formerly Symbiodinium ) or the chlorophyte Elliptochloris marina as well as being aposymbiotic. Here, we assembled a draft genome and used multi‐omics to characterise multiple physiological levels of each phenotype. We find that A. elegantissima has symbiont‐specific transcriptional and metabolomic signatures, but a similar bacterial community dominated by a single Sphingomonas species that is commonly found in the cnidarian microbiome. Symbiosis with either eukaryotic symbiont resulted in differential gene expression and metabolic abundance for diverse processes spanning metabolism and immunity to reproduction and development, with some of these processes being unique to either symbiont. The ability to culture A. elegantissima with its phylogenetically divergent photosymbionts and perform experimental manipulations makes A. elegantissima another tractable sea anemone system to decode the symbiotic conversations of coral reef ecosystems and aid in wider conservation efforts.

Background Prolonged intubation has been associated with unfavorable outcomes after cardiac surgery. A standardized approach is needed to ensure prompt extubation and shorten intensive care unit stays. Local Problem This quality improvement project was designed to evaluate the impact of a fast-track extubation protocol on time to extubation and intensive care unit length of stay. Methods The intervention group consisted of 26 adult cardiac surgery patients who underwent the fast-track extubation protocol. A Mann-Whitney test was used to compare time to extubation and intensive care unit length of stay in this group with those of a pair-matched control group of patients from the previous year who did not undergo the fast-track extubation protocol. Interventions An evidence-based literature review was used to develop a fast-track extubation protocol involving extubation in less than 6 hours. An educational activity was created to improve intensive care unit staff members’ knowledge of the fast-track extubation protocol, and its effectiveness was measured by a posttest score of 80%. Results The percentage of patients with extubation times of less than 6 hours was significantly higher in the fast-track extubation protocol group than in the pair-matched control group (U = 179, P = .003). The mean intensive care unit stay decreased from 2.92 days in the control group to 1.85 days in the fast-track extubation protocol group. Conclusion Implementing a fast-track extubation protocol for adult cardiac surgery patients shortened time to extubation and intensive care unit stay, expediting and improving recovery processes in the intensive care unit.

Sexual consent communication can be ambiguous when people rely on nonverbal, implicit cues. This ambiguity can lead to the reliance of contextual information to assess whether a sexual encounter was consensual, both in the moment and retrospectively. The current study examined how level of alcohol consumption, relationship type, and evaluator gender influenced the extent to which various sexual encounters were seen as consensual. Participants read a series of vignettes in which sexual consent was verbally granted, verbally rejected, or inferred using nonverbal cues. The vignettes also manipulated the amount of alcohol consumed by the target of the sexual advances (i.e., sober, tipsy, or intoxicated) as well as the relationship between the dyads (i.e., dating or strangers). Generally, male participants were more likely to interpret all encounters as more consensual. Encounters in which the target was intoxicated were more likely to be interpreted as nonconsensual, but instances when targets were described as tipsy (i.e., she consumed two or less alcoholic beverages) were seen as more consensual than sober encounters. The relationship between the dyads also influenced these perceptions. This work can inform educational efforts geared toward alcohol and sexual consent.

The purpose of the study was to compare heart rate (HR), systolic blood pressure (SBP), and diastolic blood pressure (DBP) following high load resistance exercise (HLRE) and blood flow restriction exercise (BFRE) with a knee wrap (kBFRE) and pneumatic cuff (pBFRE). Eleven men (N = 9) and women (N = 2) participated. HR, SBP, and DBP were collected at Rest, immediately post exercise (IP), 10-, 30-, and 45-minutes post exercise. Repeated measures ANOVAs assessed the effects of condition across time on all variables. Significant effects were examined with pairwise comparisons and a Sidak correction. Significance was defined a priori p ≤ 0.05. There were significant condition by time interactions for HR (p = 0.005; ES = 0.31), SBP (p = 0.016; ES = 0.27), and DBP (p = 0.03; ES = 0.24). There were significant main effects of time for HR (p < 0.001; ES = 0.78), SBP (p < 0.001; ES = 0.84), and DBP (p = 0.004; ES = 0.44). The HR was increased from Rest for up to 45 minutes across all conditions. IP, the HR was lower following pBFRE compared to HLRE. There was an increase in SBP at IP. SBP was higher following HLRE compared to kBFRE and pBFRE. DBP was decreased at 10 minutes across all conditions, with kBFRE prompting larger reductions compared to HLRE at 10 minutes. DBP was lower following kBFRE compared to pBFRE at 45 minutes. This study suggests that BFRE alters cardiovascular function differently than HLRE, but is dependent on cuff type. Specifically, pneumatic cuffs may attenuate the increase in the HR and SBP response, and knee wraps augment the reduction in DBP.

Parasitism is ubiquitous, yet little is known about the evolutionary mechanisms that lead to a parasitic lifestyle. Facultative parasites can switch between free-living and parasitic lifestyles, which may provide an opportunity for researchers to study the genetic mechanisms underlying a transition to parasitism. The oligochaete Dero (Allodero) hylae is a facultative parasite commonly found within the ureter of various anuran species, such as the Cuban tree frog (Osteopilus septentrionalis). The Dero hylae worm passes into the frog's cloaca, where it then infects the ureter. In the ureter, the worm loses its free-living characteristics such as hair setae, dorsal setae, a digestive tract, and fossa with gills as it transitions to a parasitic lifestyle. The parasite may be expelled from its host during urination, and when this occurs the worm will reacquire its free-living characteristics. The focus of this study was to compare the differential gene expression profiles observed when this rapid morphological change takes place. Specimens of D. hylae were extracted from wild Cuban tree frogs and either flash-frozen to capture the parasitic RNA gene expression profile or cultured for 2 wk and allowed to metamorphose to their free-living stage and then flash-frozen. The extracted RNA was used for de novo transcriptome assembly, and we conducted a differential gene expression analysis using an RNA Tag-Seq approach for both the free-living and parasitic life forms. Based on these results, we identified 213 differentially expressed transcripts between the 2 developmental forms, with 190 of these up-regulated in the free-living life form. Although over half of the differential genes recovered did not result in any significant BLAST hits, many of these genes did provide insight into which molecular signals are potentially used after the parasite transforms into the free-living form. This analysis provides significant insight into differentially expressed transcripts associated with the drastic morphological changes observed in this rare case of oligochaete parasitism by D. hylae encompassing both free-living and parasitic forms.

Lisdexamfetamine dimesylate, a prodrug stimulant, appears to effectively treat Attention-Deficit/Hyperactivity Disorder (ADHD) in children and adults. However, an analysis of the treatment effects of the two subscales (inattentiveness and hyperactivity) within the ADHD Rating Scale-IV (ADHD-RS-IV) has not yet been done to determine if clinical significance may be attributed to either one. Nor has there been a meta-analysis of the individual doses of lisdexamfetamine dimesylate. The current meta-analysis utilizes MEDLINE, Embase, Cochrane, PubMed, and clinicaltrials.gov to identify peer-reviewed studies. Selected studies were eligible if they met the following criteria: English language, randomized-controlled trials, and utilized the ADHD-RS-IV scale to assess the efficacy of lisdexamfetamine on treating ADHD in either children or adults. The primary studies utilized were published between January 2007 and April 2024. Many of these studies calculate effect sizes based on several dosages pooled together rather than by individual dosages. We conducted a random-effects meta-analysis to estimate the effect sizes for these pooled dosages on the full ADHD-RS-IV scale and its subscales, as well as to calculate effect sizes on the same scales based on the individual dosages. Our main outcome measures are the ADHD-RS-IV scale and its subscales in individual doses and pooled results in both children and adults. Adverse events during treatment were also analyzed based on stratified dosages. Eleven publications met our inclusion criteria. The analyses indicate that compared to placebo, lisdexamfetamine effectively alleviates the symptoms outlined by the ADHD-RS-IV. Moreover, there are no differences in the individual subscales or in the safety profile. Lisdexamfetamine demonstrates efficacy in treating the symptoms of ADHD, but we report that differing dosages did not yield significant differences in ADHD symptom management.

The atractid nematode Cyrtosomum penneri is an autoinfective parasite of several lizard species. Intraspecific transmission between hosts appears to occur exclusively through sexual copulation, yet it is unclear how worms are transferred between different host species. Our research aims to test the possibility of oral transmission of C. penneri using experimental infections. The lizards Anolis sagrei, Leiocephalus carinatus, Hemidactylus mabouia, and Agama picticauda were experimentally exposed to C. penneri in 1 of these groups: (1) oral infection using a feces and saline slurry to approximate host coprophagy, (2) oral infection with a large meal to approximate host predation, and (3) venereal infection using a pipette to confirm sexual transmission. Experimental infections to test venereal transmission were successful in A. sagrei, A. picticauda, and H. mabouia, but were unable to establish infections in L. carinatus. In the predation exposures, A. picticauda, A. sagrei, and H. mabouia hosted infections, whereas L. carinatus were uninfected. Finally, coprophagy experimental infections did not result in infections for any species of host. Our study corroborates venereal transmission of C. penneri in multiple species of lizards and establishes predation as an alternative route of infection. Predation as an oral route of transmission may provide C. penneri an opportunity for interspecific transmission that would otherwise be unlikely during host copulation.

With growing awareness of the environmental, economic, and social costs associated with food waste, there is a concerted effort on multiple scales to recover the nutrient value of discarded food. These developments are positive, but the rapid movement toward alternatives and the complexity of solving problems located at the intersection of economic, social, and environmental systems also have the potential to produce unanticipated risks. This paper draws upon long-term stakeholder-engaged research throughout New England, with a focus on Maine, to develop a transdisciplinary, systems-based model of the potential social, economic, and environmental risks of food waste nutrient cycling. Our effort is intended to help inform the creation of safe, functional, and environmentally benign circular food systems.