Eppendorf Group
  • Hamburg, Germany

How to optimize bioreactor inoculum in shake flasks?

30th Apr, 2020
Free Application Note: Bioreactor inoculum optimization with automated seed inoculation, conditioning, and online monitoring.
.
While monitoring and control of inoculum characteristics at the seed fermenter stage are well established in most bioprocesses, the very first steps of culture expansion in shake flasks are usually neglected, even though they are influencing all following process steps. Especially for organisms with complex metabolic regulation or morphological variability, the utilization of a suboptimal inoculum can severely worsen the bioprocess outcome. In this application note, we present an integrated approach for optimal inoculum preparation in shake flasks with automated seed inoculation, conditioning and online monitoring, which allows the generation of reproducible inoculums and the flexibility to adjust the harvesting time according to the operator’s requirements.
Image
How to optimize bioreactor inoculum in shake flasks?
Fill in the form below to download the free application note
*Required
Posted 30th Apr, 2020
1258 views
Read more from Eppendorf Group
2nd Sep, 2022

밀도 구배 울트라원심분리를 이용한 벼에서 골지체의 분리 및 농축

밀도 구배 원심분리는 특정 멤브레인의 농축에 사용되는 가장 확립된 기술 중 하나입니다.
이 애플리케이션 노트는 일련의 차동 펠릿화 및 밀도 구배 원심분리를 사용하여 벼 묘목에서 골지체 막의 분획을 얻는 기술을 설명합니다. 이 기술을 적용하면 사용자가 질량 분석과 같은 추가 다운스트림 분석을 위해 고순도 및 품질의 추출물을 얻을 수 있습니다.
자세한 내용은 애플리케이션 노트를 다운로드하십시오.
2nd Sep, 2022

密度勾配遠心法を用いたイネ苗からのゴルジ体の細胞分画工程

密度勾配遠心法は、特定の膜を濃縮するために用いられる最も確立された技術の一つです。
このアプリケーションノートでは、イネからゴルジ体の細胞分画の精製工程について、ペレッ トと密度勾配遠心法のシーケンスの技術について説明します。本技術を用いることにより、質量分析などのダウンストリーム分析に適した高純度・高品質の抽出物を得ることができます。
2nd Sep, 2022

リコンビナント抗体ホスホセリンホスファターゼの生産

組換えタンパク質の生産では、機能性タンパク質を高収率で生産することが目標となります。実験を成功させるための重要な要素には、細胞の培養と、タンパク質の分離および精製のための基本技術である遠心分離が含まれます。
このアプリケーションノートでは、エッペンドルフのシェーカーと遠心機を使って組換えタンパク質の生産に成功した例を紹介し、高品質の回収量と使いやすく時間のかからないソリューションの特質を強調しています。
2nd Sep, 2022

Free App Note: 재조합 휴먼 포스포세린 포스파타제의 생산

재조합 단백질 생산에서 목표는 높은 수율의 기능성 단백질을 생성하는 것입니다. 성공적인 실험을 위한 핵심 요소는 단백질의 분리 및 정제를 위한 기본 기술인 원심분리뿐만 아니라 세포 배양을 포함합니다.
이 애플리케이션 노트는 Eppendorf Shaker와 원심분리기를 성공적으로 사용하여 재조합 단백질을 생산하는 방법을 보여주며, 고품질의 회수량과 사용하기 쉽고 시간을 절약할 수 있는 솔루션의 특성을 강조합니다.
자세한 내용은 애플리케이션 노트를 다운로드하시고 확인하세요.
2nd Sep, 2022

단클론항체 약물 발견 문제 해결

백신과 항바이러스 약물에 대한 접근은 인구를 보호하고 환자를 치료하는 데 핵심적인 역할을 하며, 단클론항체는 COVID-19와 같이 예기치 않게 널리 퍼진 바이러스 질병에 대한 치료제로서 큰 가능성을 보여줍니다.그러나 이러한 치료법의 개발에는 여러 가지 어려움이 따릅니다.
본 eBook은 더 나은 결과, 더 빠른 처리 시간 및 고품질 제품을 달성하기 위해 이러한 과제를 해결하고 워크플로 최적화를 지원하도록 설계되었습니다.
2nd Sep, 2022

Tackling Monoclonal Antibody Drug Discovery

ワクチンや抗ウイルス剤の開発は、人々の健康や患者様の治療において重要な役割を果たします。しかしこれらの開発にはさまざまな課題があります。
このeBookは、それらの課題に対し、より良い結果、より早い開発、より高品質な製品を目指して、ワークフローの最適化をサポートするために作成されました。
9th Aug, 2022

Isolation and Enrichment of Golgi Bodies from Rice Seedlings Using Density Gradient Ultracentrifugation

Density gradient centrifugation is one of the most established techniques used for the enrichment of specific membranes.
This application note describes a technique which uses a sequence of differential pelleting and density gradient centrifugation to obtain fractions of Golgi apparatus membranes from rice seedlings. Applying this technique allows the user to gain extracts of high purity and quality for further downstream analysis such as mass spectrometry.
Download the application note to learn more.
9th Aug, 2022

Free App Note: Production of Recombinant Human Phosphoserine Phosphatase

In the production of recombinant proteins, the goal is to generate a high yield of functional protein. Key elements for a successful experiment include cell cultivation, as well as centrifugation as the basic technique for isolation and purification of the protein.
This application note demonstrates the successful use of Eppendorf shakers and centrifuges to produce a recombinant protein, underscoring the high quality yield as well as the user-friendly and time-saving qualities of the solutions.
Download the application note to learn more.
9th Aug, 2022

Tackling Monoclonal Antibody Drug Discovery

Access to vaccines and anti-viral drugs play a key role in protecting populations and treating patients, with monoclonal antibodies showing great promise as a treatment for unexpected, wide-spread viral diseases like COVID-19. Still, development of these therapies comes with an array of challenges.
This eBook is designed to help you meet those challenges and support workflow optimization in order to achieve better results, quicker processing times and higher quality products.
26th Jul, 2022

Security Measures Supporting VisioNize® Lab Suite

The lab has always been an interconnected place and cloud-based working has helped make connectivity easier and more reliable. Security is one of the main reasons for migrations into the cloud, and protecting customer data while offering cloud-based services is of the highest priority at Eppendorf. Therefore, standards and policies have been established to support the security of VisioNize Lab Suite (VNLS) and prevent unauthorized access to support the security measures of VisioNize Lab Suite (VNLS), an Eppendorf lab management solution.
Download this whitepaper to learn more about the standards, data security approaches, business practices, and certifications used for the cloud-based storage that supports VNLS and its services.
26th Jul, 2022

Digitalization in the Lab - Really a One-Way Towards More Sustainability?

Digitalization has been making a significant impact in our daily life over the last 3 years, as we have seen video conferencing become a new normal in connecting with colleagues at workplace from home. This is also true in the lab, where we are getting used to electronic lab notebooks, digital management of lab samples and connected devices in the lab. This can lead to advantages like increased efficiency, less resource consumption and contribute to a more sustainable lab, as well as challenges.
Download the white paper to learn what we should focus on to build a digitalized and sustainable laboratory.
23rd Jun, 2022

How to Remove Contamination in a Cell Culture Lab

Contamination forms a continuous risk to every cell culture lab and prevention is the key to reducing the burden of contamination.
Every lab will have different procedures for dealing with contamination. The steps outlined in this whitepaper form a good starting point for minimizing disruption and helping labs to operate efficiently.
Download the whitepaper to learn more.
11th Apr, 2022

Handling Magnetic Beads During NGS Library Prep

Next-generation sequencing sample preparation is a labour-intensive process which requires experience, precision and accuracy to generate high-quality NGS libraries.
One of the most critical steps in library preparation is the handling of the magnetic beads and it is important to do this correctly due to the following reasons:
Eliminate manual pipetting to reduce errors
Improve the reproducibility and accuracy of your sequencing data
Increase your productivity in the lab
Download the poster to learn more.
5th Apr, 2022

Handling Magnetic Beads During NGS Library Prep

Next-generation sequencing sample preparation is a labour-intensive process which requires experience, precision and accuracy to generate high-quality NGS libraries.
One of the most critical steps in library preparation is the handling of the magnetic beads and it is important to do this correctly due to the following reasons:
Eliminate manual pipetting to reduce errors
Improve the reproducibility and accuracy of your sequencing data
Increase your productivity in the lab
Download the poster to learn more.
21st Mar, 2022

Bioprocessing eBooks on Vaccine Development, Scale-Up Challenges, Digitalization and Improving Cell Culture Bioprocesses

In this free compilation of bioprocessing eBooks, gain access to best practices to improve your bioprocessing workflows along with application notes, product guides, and expert insights to bring you up to speed with the latest trends, challenges and solutions in bioprocessing.
Download the compilation to receive:
Bioprocessing: Solutions for Vaccine Development
Addressing Bioprocessing Scale-Up Challenges
Upstream Bioprocessing: Improving Efficiency Through Digital Tools
Increasing the Reproducibility of Cell Culture Bioprocesses
21st Mar, 2022

Bioprocessing eBooks on Vaccine Development, Scale-Up Challenges, Digitalization and Improving Cell Culture Bioprocesses

In this free compilation of bioprocessing eBooks, gain access to best practices to improve your bioprocessing workflows along with application notes, product guides, and expert insights to bring you up to speed with the latest trends, challenges and solutions in bioprocessing.
Download the compilation to receive:
Bioprocessing: Solutions for Vaccine Development
Addressing Bioprocessing Scale-Up Challenges
Upstream Bioprocessing: Improving Efficiency Through Digital Tools
Increasing the Reproducibility of Cell Culture Bioprocesses
11th Feb, 2022

Upstream Bioprocessing: Improving Efficiency Through Digital Tools

Digitalization can increase the efficiency, quality and reproducibility of upstream bioprocessing.
This eBook explores the growing world of bioprocess digitalization including:
How the bioprocess industry can benefit from digitalization
Concrete examples of process automation
How AI can transform bioprocessing
Download the eBook to learn more.
4th Feb, 2022

Addressing Bioprocessing Scale-Up Challenges

The approval of emerging biologic-based therapeutics - such as cell and gene therapies - presents challenges for drug companies looking to scale manufacturing volumes.
This eBook explores:
New therapies presenting scaling challenges
A scale-up study using Eppendorf Fermentation Systems
A thought leader Q&A interview with Ma Sha, Head of Bioprocess Applications at Eppendorf
Download the eBook to learn more.
4th Feb, 2022

Bioprocessing: Solutions for Vaccine Development

In this COVID-19 era, unprecedented pressure has been placed on the vaccine industry due to a surge in demand for efficient and rapid vaccine technology.
This eBook will provide you with:
A deeper understanding of how the COVID-19 pandemic has changed the global vaccine development landscape.
Workflows and technologies to help advance your vaccine research.
Application notes, product guides and expert insights to support your vaccine research and development.
4th Feb, 2022

Increasing the Reproducibility of Cell Culture Bioprocesses

This eBook explores 4 key topics in relation to bioprocessing reproducibility and efficiency:
Enhancing Bioprocessing efficiencies through run reproducibility.
Strategies to increase reproducibility of cell culture bioprocesses.
pH sensor recalibration based on exhaust CO2 concentration for bioprocess transfer and scaling.
pH sensor best practices.
Download the eBook to learn more.
10th Dec, 2021

Handling Magnetic Beads During NGS Library Prep

Next-generation sequencing sample preparation is a labour-intensive process which requires experience, precision and accuracy to generate high-quality NGS libraries.
One of the most critical steps in library preparation is the handling of the magnetic beads and it is important to do this correctly due to the following reasons :
Eliminate manual pipetting to reduce errors
Improve the reproducibility and accuracy of your sequencing data
Increase your productivity in the lab
Download the poster to learn more.
1st Dec, 2021

Handling Magnetic Beads During NGS Library Prep

Next-generation sequencing sample preparation is a labour-intensive process which requires experience, precision and accuracy to generate high-quality NGS libraries.
One of the most critical steps in library preparation is the handling of the magnetic beads and it is important to do this correctly due to the following reasons :
Eliminate manual pipetting to reduce errors
Improve the reproducibility and accuracy of your sequencing data
Increase your productivity in the lab
Download the poster to learn more.
19th Nov, 2021

White Paper: Improve Your Biomass Harvesting Efficiency

Biomass harvesting is a critical process step in every workflow related to bioprocess.
Its principle is relatively simple, however this preliminary harvesting step consists of multiple time-consuming steps - any feature that simplifies the process will greatly increase workflow efficiency.
Download this White Paper to learn how you can improve your biomass harvesting efficiency.
4th Nov, 2021

White Paper: Improve Your Biomass Harvesting Efficiency

Biomass harvesting is a critical process step in every workflow related to bioprocess.
Its principle is relatively simple, however this preliminary harvesting step consists of multiple time-consuming steps - any feature that simplifies the process will greatly increase workflow efficiency.
Download this White Paper to learn how you can improve your biomass harvesting efficiency.
29th Oct, 2021

White Paper: A Guide to Improving Biomass Harvesting

Looking for a way to save time and improve harvesting efficiency? This white paper from Eppendorf has you covered.
Complete with a step-by-step guide detailing relevant workflows, discover how you can save up to 32% on processing times whether harvesting bacteria, algae, yeast, mammalian or insect cells.
Download the white paper to learn more.