Des Moines University

Regular exercise confers numerous physical and mental health benefits, yet individual variability in exercise participation and outcomes is still poorly understood. Uncovering the neurobiological mechanisms governing exercise behavior is essential for promoting physical activity and developing targeted interventions for related disorders. While genetic studies have provided insights, they often cannot account for protein-level alterations, such as changes in kinase activity. Here, we employ protein kinase activity profiling to delineate brain protein kinase activity and signaling networks modulated by acute voluntary exercise in rats. Focusing on the dorsal striatum, which governs voluntary exercise, as well as the hippocampus, which is susceptible to modulation by physical activity, we aim to understand the molecular basis of exercise behavior. Utilizing high throughput kinome array profiling and advanced pathway analyses, we identified protein kinase signaling pathways implicated in regulating voluntary exercise. Pathway analysis using Gene Ontology (GO) revealed significant alterations in 155 GO terms in the dorsal striatum and 206 GO terms in the hippocampus. Changes in kinase activity were observed in the striatum and hippocampus between the exercise (voluntary wheel running, VWR) and sedentary control rats. In both regions, global serine-threonine kinase (STK) activity was decreased, while global phospho-tyrosine kinase (PTK) activity was increased in VWR rats compared to control rats. We also identified specific kinases altered in VWR rats, including the IKappaB Kinase (IKK) and protein kinase delta (PKD) families. C-terminal src Kinase (CSK), epidermal growth factor (EGFR), and vascular endothelial growth factor receptor (VEGFR) tyrosine kinase were also enriched. These findings suggest regional heterogeneity of kinase activity following voluntary exercise, emphasizing potential molecular mechanisms underlying exercise behavior. This exploratory study lays the groundwork for future investigations into the causality of variations in exercise outcomes among individuals and different sexes, as well as the development of targeted interventions to promote physical activity and combat associated chronic diseases.

The recruitment of qualified family medicine faculty offers significant opportunities and challenges. Attention to diversity, equity, and inclusion and the fostering of a culturally diverse climate is vital to robust family medicine faculty recruitment and retention. An inclusive recruitment framework for diversifying graduate medical education (GME) training programs includes building on strong institutional support by setting diversity as a priority, implementing inclusive recruitment practices, seeking out suitable candidates, investing in trainee success, and building the pipeline. The program director must have a vision for the program that is aligned with that of the institution and which cultivates and guides each member of the institutional team in shared ownership and synergistic teamwork toward the program’s success. Residency leadership can benefit from maintaining a mindset that they are always recruiting faculty, even when recruiting students, where mentorship and role modeling efforts can help expand students’ imagination of what an academic career can look like. Specific benefits and challenges are outlined for faculty members transitioning from prior clinical practice, new residency graduates joining faculty as freshly minted attendings, and graduates retained by their own program through internal faculty recruitment efforts.

Forensic anthropology must embrace standards and credentialing that align with other medicolegal disciplines, such as forensic pathology and forensic odontology. This involves establishing itself more firmly as an essential specialty in the medicolegal system and limiting practice to qualified professionals. Without educational or professional requirements to practice forensic anthropology, the competencies of those involved in medicolegal cases vary, despite the potentially serious legal impact of errors. Negligent work can impede decedent identification or wrongly convict or free a suspect. Erroneous and unprofessional work also misrepresents the field and may deter agencies from utilizing forensic anthropologists. This commentary aims to convey the need to further professionalize forensic anthropology through the standardization of education, qualifications, and practice. Differences between certification and licensure and their implications are discussed. Recent and upcoming developments in the field highlight progress, including the development of a multi‐level certification system and list of core competencies by the American Board of Forensic Anthropology (ABFA). Additionally, the Anthropology subcommittee of the Organization of Scientific Area Committees for forensic science (OSAC) is developing a standard for qualifications in forensic anthropology. These developments emphasize the importance of certification and standardization in education and training for the advancement of forensic anthropology. Standardization efforts such as these will foster a cohesive, respected practice to serve the evolving needs of the medicolegal system.

Introduction Black Americans suffer from disproportionately high rates of cardiovascular disease (CVD). Psychosocial stress contributes to this disparity. Previous studies reported that the Transcendental Meditation (TM) technique reduced CVD risk factors, surrogate endpoints, and clinical events in high-risk populations. However, no study has evaluated the effects of stress reduction with meditation on surrogate CVD markers such as carotid intima-media thickness (cIMT) along with CVD clinical events. Therefore, this randomized clinical trial evaluated the long-term effects of meditation and health education (HE) on cIMT and CVD events in high-risk Black adults. Materials and methods Participants were Black women and men with CVD or at high risk who were randomized to either TM or HE. The primary outcome was a change in cIMT measured using B-mode ultrasound at baseline and 12 months. The main secondary outcome was major adverse cardiovascular events (MACE) at 5 years (maximum) of follow-up. Other secondary outcomes were MACE at 1 and 10 years of follow-up, blood pressure, and serum lipids after 1 year. Exploratory variables were a comparison of cIMT changes to historical controls and MACE after 14 years. Results There were 197 randomized participants, of whom 136 completed posttest for cIMT. After 1 year, the TM and HE groups showed average cIMT changes of −0.0004 and −0.0003 mm, respectively, with no significant difference between the groups. Additionally, there were no significant differences between the groups in lipid levels or BP. However, both TM and HE groups showed prevention of progression of cIMT compared to historical controls at 12 months. In the survival analysis of MACE, there was a 65% relative risk reduction in the TM group after 5 (maximum) years of follow-up (HR = 0.346; 95% CI = 0.134–0.893; p = 0.017). At 1 and 10 years of follow-up, there were significant risk reductions in the TM vs. HE group, which was not significant at 14 years (all yearly maximums). Discussion Both treatment groups demonstrated prevention of progression of cIMT over 12 months compared to historical controls. However, the TM group showed a relative risk reduction for MACE of 65% at 5 years. Therefore, as a lifestyle modification method, TM may be useful in the secondary prevention of CVD in this and possibly other high-risk groups. Clinical trial registration ClinicalTrials.gov, NCT05642936.

Introduction Community Health Workers (CHWs) play a crucial role in extending health services, particularly for people who are medically underserved. Despite efforts to expand CHW programs nationally and in Iowa, challenges persist in defining their roles and responsibilities. Few studies have considered the perspectives of both CHWs and CHW employers simultaneously. Methods We conducted an exploratory sequential mixed-methods study, first involving key informant interviews with CHWs and employers, which then informed the development of surveys distributed to both populations. We performed thematic analysis of qualitative data and calculated descriptive statistics of quantitative data. Results Key informant interviews were conducted with five CHWs and five employers. An additional 123 CHWs and 81 employers responded to the survey. From the interviews, we report six themes, including roles and responsibilities, interaction with the broader health care team, and support needed. Survey respondents reported 69 unique job titles, a wide range of populations served, and diverse training needs. Despite 93.6% (n = 102) of CHWs receiving on-the-job training, 48% (n = 52) indicated they would still benefit from more training to be effective in their roles. 46% (n = 21) of employers reported unstable funding as a major barrier to program implementation. Discussion CHWs in Iowa felt supported and valued as members of the care team, yet challenges to growing the CHW workforce remain. Our findings highlight the need for continued role definition of the CHW workforce, as well as the need to establish more sustainable sources of funding to ensure the continuity and expansion of this health equity-enhancing workforce.

Non-human vocalizations carrying information regarding external events have been likened to referential words and are thus integral for exploring the origins of linguistic reference. Previous research suggests receivers decode this referential information and some studies have indicated that such calls can, like in humans, evoke mental representations of the referent in receivers. However, the nature of these representations remains ambiguous. Specifically, whether calls elicit affect-based representations (e.g. signaller fear after alarm calls) or object-based representations (e.g. threats encountered by signallers after alarm calls), or both, in listeners remains untested. To investigate this, we conducted a match-to-sample task with a language-competent bonobo (Kanzi) asking him to match playbacks of conspecific alarm and food-associated calls to lexigrams representing either affect-based (‘scare’, ‘surprise’) or object-based (‘snake’, ‘food’) content. Kanzi matched alarm calls to ‘scare’ and ‘snake’ lexigrams at above chance levels regardless of caller familiarity but did not match food-associated calls to either ‘surprise’ or ‘food’ targets. We propose environmental cues are required to interpret food-associated calls that occur across a variety of contexts. These findings suggest bonobo alarm calls evoke object- and affect-based representations for Kanzi, indicating the mechanisms underlying the perception of non-human vocalizations may be more similar to those in language than previously thought.

The recent scoping review by Tomescu et al. on the effects of rurality on head and neck cancer brings a helpful focus to an important topic. There are many disparate effects of rurality on treatment and outcomes that must be better studied in a targeted manner.

The phylogenetic contextualization of human neuroanatomy is crucial for understanding the positive, neutral, and/or negative effects of therapeutic interventions derived from animal models. Here we determined the parcellation of, and quantified, orexinergic (or hypocretinergic) neurons in the hypothalami of humans and several species of primates, including strepsirrhines (two species), platyrrhines (two species), cercopithecoids (three species), and hominoids (three species, including humans). The strepsirrhines, platyrrhines, and cercopithecoids presented with three distinct clusters of orexinergic neurons, revealing an organization like that observed in most mammals. In the three hominoids, an additional orexinergic cluster was found in the tuberal region of the hypothalamus, termed the optic tract cluster extension. In humans only, an additional parvocellular cluster of orexinergic neurons was observed in the dorsomedial hypothalamus. The human presented with the most complex parcellation of orexinergic neurons of the primates studied. Total numbers of orexinergic neurons in nonhuman primates were strongly correlated to brain mass (Puncorr = 1.2 × 10−6), with every doubling in brain mass leading to an ∼1.5-times increase in neuron number. In contrast, humans have approximately 74,300 orexinergic neurons, which is significantly less than the 205,000 predicted using the nonhuman primate regression for a brain mass of ∼1363 g. We conclude that although the human orexinergic system is the most complex of primates in terms of parcellation, with potential associated functional specializations, this system is quantitatively paradoxical in having a significantly lower neuronal number than expected for a primate with an ∼1363-g brain.

Background Individuals aged 15–24 years are disproportionately affected by sexually transmitted infections (STIs) in the United States, and young women can face severe health consequences if STIs are left undiagnosed. Yet STI testing rates in this priority population remain low. Few studies incorporate health behavior theory to examine factors associated with STI testing among young women in the United States, which is vital for intervention development. This study therefore sought to identify the determinants of college-enrolled late adolescent women’s intentions to be tested for common STIs in the next 12 months using a theory-based approach. Methods Students who identified as female at a large Midwestern university completed an online survey about STIs (n = 171). Hierarchical multiple regression was used to assess the relationship between STI testing intention and the Reasoned Action Approach (RAA) global constructs of attitude, perceived norm, and perceived behavioral control, supplemented by STI knowledge, STI stigma, and STI shame. Results Findings indicate that the RAA global constructs predicted STI testing intention (P < 0.001), with perceived norm making the largest contribution. Neither STI knowledge, stigma, nor shame were significantly associated with STI testing intention. Conclusions Future interventions aimed at increasing STI testing among young women should focus on beliefs underlying the RAA global constructs and especially young women’s beliefs regarding other people’s support for getting tested for STIs.

Osteosarcoma is a highly aggressive cancer in children and young adults that has a remarkably high mortality rate upon metastasis. Current standard treatments have remained largely unchanged for nearly five decades, focusing on a combination of chemotherapy with high-dose methotrexate, doxorubicin, and cisplatin, complemented by aggressive surgical resections. Despite this lack of change, recent advancements in medical research have spurred hope for more effective and less invasive approaches to managing osteosarcoma. In this review, we provide an overview of existing therapeutic modalities, including chemotherapy regimens tailored to tumor stage and patient response, radiation therapies aimed at local tumor control, and advanced surgical techniques such as limb-sparing procedures. Additionally, we explore two promising future treatments that are currently under investigation for osteosarcoma cases: targeted therapies utilizing nanomaterials like graphene oxide and innovative oncolytic viruses. This review highlights potential breakthroughs in treatment options while identifying areas that warrant further investigation in the management of osteosarcoma. Considering the limited advancements in treatment over the past decades, identifying and highlighting novel and effective therapies is vital for improving patient outcomes and survival rates.

Skin cancer, including melanoma and non-melanoma types, presents a significant and growing global health challenge due to its increasing incidence and mortality rates. While conventional treatments such as surgical excision, immunotherapy, and targeted therapies are well-established, microorganism-based approaches represent an innovative and promising alternative. These therapies employ live, genetically engineered, or commensal bacteria, viral vectors, or bacterial components to achieve various therapeutic mechanisms, including tumor targeting, immune system modulation, vascular disruption, competitive exclusion, drug delivery, and direct oncolysis. Despite their potential, these approaches require further investigation to address safety concerns, optimize treatment protocols, and gain a comprehensive understanding of their long-term outcomes.

Since its discovery, the natural endocast of the Taung cranium has played a central role in the interpretation of human brain evolution. Aspects of the endocast including the identification of the lunate sulcus, possible expansion of the parietal lobe, and rounded profile suggested to R. Dart that the Taung individual was aligned with humans and not with other anthropoid primates, yet these interpretations were immediately controversial and remain so today. We have generated a detailed curvature map of the Taung endocast to evaluate its surface organization with reference to 189 chimpanzee and 20 human brains. These data enable evolutionary consideration of the surface detail of depressions and projections sufficient to mark primary sulci and variations in sulcal organization due to superficial bridges between adjacent gyri. Our results suggest that the lunate sulcus in the Taung endocast displays a gyral bridge between the occipital lobe and the inferior parietal lobule seen in 65% of our adult human brain hemispheres but in only 1.8% of our chimpanzee ones. The frontal lobe organization of the Taung endocast reflects a superior frontal sulcus pattern seen in 92.8% of our adult human brain hemispheres, but in 0% of our adult chimpanzee sample, and an inferior frontal sulcus pattern seen in 100% of our adult human brain hemispheres but in only 2.1% of our chimpanzee ones. The Taung inferior frontal gyrus retains a fronto-orbital sulcus which is seen in 0% of our adult human brain hemispheres and in 100% of our adult chimpanzee ones. These observations help to resolve some apparent inconsistencies of interpretation of the posterior endocast of the Taung specimen while showing that the specimen shared some derived aspects of endocast organization with humans that were not found in chimpanzees.

Asthma has long been associated with increased susceptibility to viral respiratory infections, leading to significant exacerbations and poorer clinical outcomes. Contrarily and interestingly, emerging data and research surrounding the COVID-19 pandemic have shown that patients with asthma infected with SARS-CoV-2 experienced decreased severity of disease, lower hospitalization rates, as well as decreased morbidity and mortality. Research has shown that eosinophils could enhance immune defense against viral infections, while inhaled corticosteroids can assist in controlling systematic inflammation. Moreover, reduced ACE-2 expression in individuals with asthma may restrict viral entry, and the Th2 immune response may offset the Th1 response typically observed in severe COVID-19 patients. These factors may help explain the favorable outcomes seen in asthmatic patients during the COVID-19 pandemic. This review highlights potential protective mechanisms seen in asthmatic patients, including eosinophilia, the use of inhaled corticosteroids, reduced ACE-2 expression, and a dominate Th2 immune response. Such a study will be helpful to better manage patients with asthma who have contracted COVID-19.

The purpose of the study was to determine the relationship between anger, hostility, physical aggression, verbal aggression, and emotional forgiveness (positive emotional forgiveness and reduction of negative emotions) in a longitudinal study design. Data were analyzed from 366 juveniles aged 10–18 (M = 15.18, SD = 1.63) who completed questionnaires during two waves. The procedure for both measurements consisted of completing questionnaires measuring emotional forgiveness and aggression. In order to achieve the aim of the study, we decided to evaluate a partial cross-lagged model. Our analyses showed that the reduction in negative emotions toward the offender at T1 was a significant predictor of anger and hostility at T2. Sex was a significant covariate for verbal aggression, anger, and hostility. This study adds to the current literature by supporting previously theorized relationships. It suggests the possibility of training forgiveness skills in juveniles, reducing aggressive and potentially delinquent behavior, especially in response to injustice and events that trigger anger and hostility.