Dalian Polytechnic University
  • Liaoning province, China
Recent publications
The improvement of catalysts’ stability under harsh reaction conditions is vital for their practical applicability. Herein, iron carbide (Fe3C) nanoparticles were encapsulated in graphitic carbon in situ and a carbon ball served as the carrier. The synthesized Fe3[email protected]/C was first utilized to treat an m-cresol wastewater containing Si via catalytic ozonation. Compared with the commercial Fe/Al2O3 catalyst, the resistance to Si of the Fe3[email protected]/C was improved 22.68 times, while the TOC removal rate increased by a factor of 2.9, and it remained stable during 10 cycles and 12000 min of continuous reaction, which further demonstrated its potential for diverse applications. The catalyst exhibits improved resistance to Si because of the dual protection from the carbon-encapsulated structure and carbon carrier. Density functional theory calculations show that the encapsulation of Fe3C using carbon significantly increases the resistance to adsorption of Si on its active sites. In addition, the activation of O3 is unimpeded on the Fe3C adsorption sites by the protection from C, thus the generation of reactive oxygen species (ROS) by ozone is largely promoted. The mechanism associated with the resistance of the Fe3[email protected]/C catalyst to Si and its elevated activity are also elucidated.
A green combinatorial pretreatment using liquid hot water (LHW) and lactic acid (LA) was used to treat reed straw for effective lignocellulose digestibility and xylose recovery. The reeds were first treated with LHW at 180 °C for 60 min, followed by LA treatment at 170 °C for 45 min. Using 37.5 g/L lactic acid, the lignocellulose digestibility was 80.6 % and glucose yield was 56.0 %, which was much higher than that for one-step LHW pretreatment (the lignocellulose digestibility was 27.3 % and the glucose yield was 26.3 %). These data demonstrated that LHW-LA pretreatment increased lignocellulose digestibility and glucose yield by 1.95-fold and 1.13-fold, respectively. The highest total sugar yielded 39.2 g/100 g-substrate. The results revealed that LHW-LA pretreatment removed most hemicellulose and partial lignin from the reed straw and prompted cellulase binding to reed cellulose, which enhanced lignocellulose enzymatic saccharification. The high fermentable glucose yielded from the combinatorial pretreatment as presented in this study highlighted the great potential of our pretreatment technology to be applied in the biorefinery, which could lay down the cost and sustainability of current biorefinery industry.
This study aimed to investigate the protective effect of mussel polysaccharide (MP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice. MP was shown to stimulate secretion of cytokines (SIgA, IL-2, IF-γ, IL-4, IL-10) and production of transcription factors (occludin, claudin-1, ZO-1, mucin-2, IL-2, IF-γ, IL-4, IL-10). Key proteins ( p -IκB-α, p -p65) of the NF-κB pathway were upregulated after MP administration. SCFAs levels, which were decreased after the Cy treatment, were improved after treatment with MP. Furthermore, 16 S rRNA sequencing data of fecal samples revealed, through α-diversity and β-diversity analysis, that MP improved microbial community diversity and modulate the overall composition of gut microbiota. Taxonomic composition analysis showed that MP increased the abundance of probiotics species ( Lactobacillus ) and decreased the proportion of pathogenic species ( Desulfovibrio ). These findings suggested that MP has a potential immunomodulatory activity on the immunosuppressive mice.
Progranulin (PGRN) is an autocrine growth factor that regulates cell proliferation, migration, wound healing, and tissue repair in mammals. Lamprey is the most primitive of the extant vertebrates and is regarded as the survivor of a once flourishing group of paleozoic vertebrates, with a history of more than 500 million years. To date, the evolutionary dynamics and the underlying function of the PGRNs remain largely unclear in lamprey. Here, we screened four genes encoding PGRNs from the genomes of Lethenteron reissneri and Petromyzon marinus , including one long form (named Lr-PGRN-L) and three short forms (named Lr-PGRN-S1, Lr-PGRN-S2, and Lr-PGRN-S3), and performed phylogenetic tree, functional domain, and synteny analyses to identify the evolutionary history of the four Lr-PGRNs. In addition, the expressions of the four Lr-pgrn family genes and the immune response against various pathogenic challenges were also investigated. We found that these genes were widely distributed in various tissues of lamprey and performed a variety of functions. Moreover, our results suggest that Lr-PGRN-S1 induces cell migration and proliferation, and is involved in repair after skin and spinal cord injury under appropriate conditions. Our findings are valuable because they improve the understanding of the evolutionary relationship of vertebrate pgrn genes, as well as providing new insights into the diverse and important roles of Lr-PGRNs.
As for the structural design of photocatalytic materials, it is possible to prolong the recombination time by constructing heterojunctions to drive photogenerated carriers due to the preference between energy levels. However, the separation of transported electrons and holes appears only for the underlying physical properties and photocatalytic activity of the catalyst. The synergistic design of heterojunctions, including surface defect engineering as well as electron storage and delayed release in catalysts, has been rarely reported. In this work, a photocatalyst that loading TiO2 on multilayer CoOOH was reported, achieving the modulation of the oxygen vacancies existing on the TiO2 surface based on the heterojunctions. Compared with TiO2 loading on Co3O4, more oxygen vacancies are available, and the stored photogenerated electrons are released one after another through the multilayer structure. In the photocatalytic oxidation of rhodamine-B (Rh-B), the activity is enhanced to four times comparing with that of TiO2. As a novel and unique structural design, the extraordinary performance deserves to be approved, and it is promising to be further investigated in the future.
A comprehensive method was applied to evaluate the anticoagulant activity of a novel anticoagulant peptide (NAESLRK) derived from oyster (Crassostrea gigas). The anticoagulant peptide drastically reduced the extrinsic clotting activity and also impaired the intrinsic clotting activity slightly. Consistent with clotting data, the thrombin peak height was reduced to 84.7 nmol/L from 123.4 nmol/L, and thrombin generation time was delayed to 4.67 min from 4.42 min when the extrinsic trigger was applied. The inhibitory kinetics of FXIa, FIXa, FXa, FIIa, and APC in a purified component system rationally explained the reduction of extrinsic clotting activity and impairment of thrombin generation. Besides the inhibition of FXa and FIIa activity, the activation processes of FX and FII by intrinsic/extrinsic tenase complex and prothrombinase were also damaged. The anticoagulant activity in the plasma system was the result of comprehensive inhibition of various factors. The research provided a novel method for anticoagulant evaluation and inhibitory mechanism of bioactive peptides from food products.
Phenolic derivatives from lignocellulosic biomass under harsh pretreatment conditions may inhibit microorganisms. Waste liquid produced from alkali pretreatment of sugarcane bagasse (SCB) was qualitatively and quantitatively analyzed. Model compounds were used to evaluate the effects of major derivatives of alkali pretreatment liquid (APL) on the growth and succinic acid (SA) synthesis of Actinobacillus succinogenes ATCC 55618, and molecular mechanisms were analyzed with transcriptome and proteome sequencing. The 10 most abundant measured components in APL were all lignin degradation products, and they showed different degrees of inhibitory effects on A. succinogenes. Benzoic acid and p-hydroxy benzaldehyde exhibited most serious inhibition to A. succinogenes, causing biomass and SA concentration to decrease by over 30 % and 45 %, respectively. This could be attributed to the presence of inhibitors caused the down-regulation of genes including phosphofructokinase, xylulose kinase, pyruvate carboxylase, and acetate kinase, which are crucial in pathways for sugar uptake, CO2 fixation, and reducing power supply.
Based on first-principle calculations, the magnetic properties and ferromagnetic origins of Ca(Zn, Cr)2As2 systems with and without vacancy were investigated. The Cr-doped CaZn2As2 systems were magnetic, and eight Ca8(Zn14Cr2)As16 configurations in terms of distances between Cr pairs preferred ferromagnetic state. The magnetic moments mostly came from the Cr-3d states strongly hybridized with the As-4p states around the Fermi level. Compared with the Ca8(Zn14Cr2)As16 system, the ferromagnetic state of p-type Ca8(Zn14Cr2)As16 with a VCa or VZn system was more stable. The ferromagnetic stability of n-type Ca8(Zn14Cr2)As16 with VAs was reduced. These calculated results could provide theoretical guidance for further experimental research.
Ag-aggregates and Mn²⁺ doped germanate glasses were prepared by a melt-quenching method, and their spectroscopic and chromatic properties were studied. It was found that Ag-aggregates doped samples exhibited broadband emission covering almost the visible spectral range. When Mn²⁺ with ⁴T1(⁴G) to ⁶A1(⁶S) transition was introduced, the broadband emission of Ag-aggregates doped germanate glass was further extended in red light range. Meanwhile, the luminescence color coordinate and correlated color temperature (CCT) of Ag-aggregates/Mn²⁺ co-doped sample excited by 360 nm were derived to be (0.33, 0.32) and 5616.7 K, respectively, and its color rendering index (CRI) is up to 93. Moreover, the optical gain at various wavelengths was investigated based on an amplified spontaneous emission (ASE) technique, and the Ag-aggregates/Mn²⁺ co-doped sample possesses broadband optical gain in full visible range. These results indicate that Ag-aggregates/Mn²⁺ co-doped germanate glasses are promising candidates for applications in white light-emitting-diodes (wLEDs) and broadband tunable lasers.
Renewable and cheap reed roots are rich in polysaccharide and lignin and naturally possesses wastewater purification ability. However, if applied as industrial adsorbent, the active sites of reed roots need further enhancement to fit realistic amphoteric ion adsorption requirement and its recovery also request for optimization to avert secondary pollution caused by adsorbent residues. Under this background, a novel adsorbent based on reed root loaded with magnetic amino-functionalized nanoparticles (RrH@Fe3O4 @SiO2-NH2) with a bionic dendritic structure and a pleated surface was designed. The reed roots were firstly pretreated with hydrogen peroxide solution with strong oxidizing properties under UV light irradiation to expose more oxygen-containing functional groups, which then condensed with the amino groups on the surface of Fe3O4 @SiO2-NH2 nanoparticles, leading to the formation of the final adsorbent. The carboxyl, hydroxyl and methoxy and amino groups on the surface of the adsorbent synergistically contribute to the adsorption of both anions and cations. The maximum adsorption capacity for Pb (II) and Cr (VI) ions per unit mass of reed root in RrH@Fe3O4 @SiO2-NH2 reached 99.1 and 106.5 mg g− 1, much higher than those obtained with raw reed roots (24.8 and 30.6 mg g⁻¹). The superior adsorption performance was ascribed to its physicochemical properties as characterized by a series of characterization methods. The adsorption process followed pseudo-second-order kinetic and Freundlich isotherm model, indicating a chemical multi-layer endothermic adsorption process. The addition of magnetic Fe3O4 endows the easy recovery of the adsorbent by applying a magnetic field, and after five cycles, the adsorption capacity can still reach 82% of that of the first time. In summary, the desirable adsorption performance, easy recovery, and low cost make the bionic RrH@Fe3O4 @SiO2-NH2 a promising amphoteric adsorbent for eliminating heavy metal ions from wastewater.
Fermentation cost and oxygen-mediated lipid peroxidation have become economic and technical bottlenecks for the commercialization of docosahexaenoic acid (DHA) from microorganisms. Yacon (Smallanthus sonchifolius) tuber is rich in fructan, phenolics and other nutrients, and it represents a low-cost and abundant feedstock for microbial fermentation. Herein, yacon tuber hydrolysate (YTH) yielded by exo-inulinase was used as a novel potential substrate for Schizochytrium sp. ATCC 20888 to produce DHA. Under the optimized medium composition of reducing sugar of 100 g/L, C/N ratio of 30 and C/P ratio of 200, the maximal biomass, lipid and DHA yield in YTH medium reached 50.60, 24.44 and 10.85 g/L, respectively, which were much higher than those produced with glucose and fructose. Antioxidant capacity and enzyme activity analysis showed that the superior fermentation performance using YTH was mainly attributed to the significant increase in the activities of related key lipogenic enzymes, the reduction in intracellular reactive oxygen species and the inhibition of lipid peroxidation. Therefore, yacon tuber was proven to be an ideal alternative feedstock for efficient high-value DHA production by Schizochytrium sp.
Design a novel and efficient adsorption-reutilization system is required urgently to overcome the difficulty of heavy metal-polluted water and secondary pollution of adsorbent-heavy metals. Here, a functional imitation wood-derived adsorbent was fabricated with carboxyl-functionalized ordered mesoporous tube bundles (named as [email protected]), which was successfully used in the high efficiency removal of heavy metal and then reused in chemiluminescence after adsorption. Experimental results indicated that high-density carboxyl groups were grafted onto the ordered mesoporous tube bundles of the delignified parasol via chemical modification, resulting in an efficient adsorption toward various heavy metal from wastewater. More importantly, the adsorbed spent adsorbent (M²⁺@[email protected]) was reused for the first time to act as heterogeneous catalytic cites for the chemiluminescence via an ingenious strategy in the system of N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and H2O2. The prepared chemiluminescence system presented high intensity (1.42 ×10⁶ a.u.) and long duration time (over 10 h), which avoids the traditional secondary pollution and waste reprocessing. In view of the low price, easy availability and renewable of raw materials, the simple facile fabrication process, and the high efficiency of adsorbent as well as the interesting strategy for successive application in adsorption and chemiluminescence, the [email protected] will potentially bring about high speed development and widespread applications in wastewater treatment, biological imaging and cold light sources.
The development of hydrogel-based photocatalysts with three-dimensional ordered porous structure, fast charge separation, and low resistance is significantly in photocatalytic biorefinery but nonetheless poses certain challenges. Herein, we introduced a novel strategy to prepare “Fish gill”-shaped hydrogel-based photocatalysts via freezing-induced encapsulation of ultra-thin carbon nitride nanosheets into a three-dimensional ordered porous polyvinyl alcohol matrix (name as FG-s[email protected]x). The encapsulation of CNNS in FG-s[email protected]3 posed no effects on the absorption of visible light but accelerated the transfer/migration of photo-induced charge and reduced the resistance. For photocatalysis, the ordered porous structure of FG-s[email protected]3 proved to be conducive to the reactants adsorption and mass transfer while the lactic acid yield achieved 92.6%. FG-s[email protected]3 exhibited excellent stability and reusability, in which the yield of the 10th cycle well reached 95.6% of its first cycle. FG-s[email protected]3 also displayed exceptional universality wherein the lactic acid yields were 92.6% (glucose), 89.1% (fructose), 88.6% (mannose), 56.0% (rhamnose), 71.1% (arabinose), and 70.9% (xylose). In addition, the effects of various oxidative species on the photocatalytic reaction were systematically discussed, among which ·O2⁻ played a pertinent role. Overall, the construct of hydrogel-based photocatalysts and their application provide a strategy for photocatalytic biorefinery.
This study aimed to investigate the effect of the oral administration of sea cucumber protein (SCP) on wound healing. SCP was isolated and purified from the body wall of Stichopus japonicus. A mouse skin incision model was operated on to evaluate the wound repair effect of SCP. The histological changes in the skin at the wound sites of BALB/c mice were observed by staining with haematoxylin and eosin (H&E) and Masson's trichrome. The enzyme-linked immunosorbent assay (ELISA) was used to analyze the expression of inflammatory cytokines in BALB/c mice. The boost cell migration ability was detected by a scratch assay after HaCaT cells were cultured with digested SCP (dSCP). Western blotting and RT-PCR assays were performed to determine the mechanism of SCP promoting wound healing. As a result, the wound healing rate in the SCP high dose group was 1.3-fold, compared to that in the blank group on day 14. Also, increased epidermal thickness and 1.79-fold collagen deposition contrasted with the blank group. Additionally, SCP could up-regulate the levels of pro-inflammatory factors (IL-1β, IL-6, TNF-α) from day 3 to 7 firstly and decreased from day 7 to 14. IL-8 expression continuously decreased while the level of anti-inflammatory factor (IL-10) increased during the healing stage. Furthermore, the cell closure area reached 67% after being treated with 50 μg mL-1 of dSCP for 48 h. Cell proliferation was associated with the dSCP-activated PI3K/AKT/mTOR pathway. Taken together, SCP can be orally used as an effective agent for wound repair.
Conventional optical anti-counterfeiting strategies are based on the single-color emission, which are easily deciphered and thus greatly limited in the application of information security. Herein, a multimodal dynamic optical information coding with red, green, and blue (RGB) tricolors has been developed by photoluminescence (PL), persistent luminescence (PersL), thermally stimulated luminescence (TSL), and thermally stimulated persistent luminescence (TSPL). The BaSi2O2N2:Eu2+ phosphors with a blue emission peak at 494 nm were used as the crucial blue optical information coding material and exhibited the distinctive response properties to light, heat, and force stimuli with intrinsic trap depths of 0.674 and 0.82 eV. More importantly, by combining the red Sr2Si5N8:Eu2+,Dy3+ and green SrSi2O2N2:Eu2+,Dy3+ nitride phosphors, a RGB tricolor and multimodal strategy has been successfully developed for anti-counterfeiting applications. The "RGB tricolor flower" with RGB emissions is given as a typical example to achieve the dynamic display of optical information encryption and decoding through the various PL, PersL, TSL, and TSPL modes. Finally, the traditional quick response (QR) code mechanism has been integrated into the design of multi-information encrypted RGB tricolor anti-counterfeiting devices with different identifiabilities of the encrypted information in natural light, PL, PersL, TSL, and TSPL modes. The laminated layers of RGB QR code patterns containing different specific information, such as "DLPU" and "116034", can be effectively recognized in the corresponding modes. The design strategy of RGB tricolor and multimodal optical information encryption and decoding devices in this work greatly improves the security level of advanced optical information technologies and extends the potential applications in dynamic anti-counterfeiting fields.
Biomechanical Energy Harvesters In article number 2204304, William W. Yu, Wen Jung Li, Walid A. Daoud, and co‐workers demonstrate a high‐performance flexible biomechanical energy harvester by switching its interfacial adhesion via hydrogen bonding interactions and solvent evaporation induced phase separation. This wearable power source can generate a record high peak power density of 20.5 W m−2 Hz−1 under a low biomechanical input of 5 N, and can sufficiently power small electronics through harvesting regular or intermittent human motions.
Hyperlipemia is becoming a chronic disease that threatens human health. At the same time, people pay more and more attention to hyperlipemia. Holothuria Leucospilota polysaccharide (HLP) has been reported to ameliorate hyperlipidemia in high‐fat diet‐induced rats. Therefore, this study aimed to explore further metabolomics' role in improving liver function and reveal its mechanism. After oral administration of HLP for 4 weeks, total cholesterol (TC) and triglycerides (TG) levels of the liver in 100 and 200 mg/kg HLP groups were both decreased significantly (p < .05). The results showed that serum AST and ALT activity decreased by professing to be convinced of HLP. HLP also exerted antioxidant activities and up‐regulated the expression of ACC, CD36, TNF‐α and NF‐κB in the liver of diabetic rats. Six potential biomarkers were recognized by UPLC‐Q‐TOF/MS and OPLS‐DA. HLP alleviated liver injury by regulating the contents of metabolic end products in the serum of hyperlipidemic rats, such as nadolol and glycodeoxycholic acid. The results indicated that HLP effectively relieved HFD‐induced hyperlipidemia by regulating metabolic disorders. Practical applications As a chronic disease, hyperlipidemia has attracted more and more attention. Studies have shown that HLP regulates dyslipidemia, oxidative damage and inflammation to relieve hyperlipidemia. It mainly improved the liver damage caused by hyperlipidemia by inhibiting the expression of hepatic lipogenesis, oxidative stress and inflammatory factors. At the same time, we also detected six metabolites, among which high GDCA content indicated serious liver damage. Therefore, in the future, it can be suggested that HLP may be used as a functional, active substance in health products to assist in relieving hyperlipidemia, and GDCA may be used as an essential metabolic marker for the degree of liver injury.
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239 members
Ling-Ping Xiao 肖领平
  • Liaoning Key Lab of Lignocellulose Chemistry and BioMaterials
Xianzhen li
  • School of Biological Engineering
Jinfeng Pan
  • Department of Food Science and Technology
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Liaoning province, China