Chinese PLA General Hospital (301 Hospital)
Recent publications
Background Primary headache and psychiatric diseases are bidirectional correlated. The real-world data of depression and anxiety in Chinese patients hospitalized for primary headache, considering all subtypes, remain unclear. Methods This study enrolled patients attending eight Chinese headache centers from October 2022 to September 2023. A WeChat mini-program was designed to collect data. Headache was diagnosed and confirmed by two headache specialists. The Patient Health Questionnaire-9 and Generalized Anxiety Disorder-7 were used to assess depression and anxiety. Results Overall, 1963 patients with primary headache were analyzed; the prevalence of depression and anxiety was 20.1% (396/1963) and 14.8% (290/1963), respectively. Of the 1963 patients, 217 (11.1%) had history of anxiety or depression and 184 (9.4%) had undergone assessments. Patients with both primary headache and depression were more likely to be women (77.8% vs. 71.9%), experience more severe headache (numerical rating scale; 6.2±1.9 vs. 5.7±1.9) and greater impacts on quality of life (Headache Impact Test-6; 65.3±8.5 vs. 58.1±11.5). Those with both primary headache and anxiety exhibited similar results and were less educated. Depression and anxiety were more prevalent in chronic migraineurs (CM) than in episodic migraineurs (36.8% vs. 16.9% and 28.9% vs. 12.3%, respectively) and in those with chronic (CTTH) than in those with episodic tension-type headache (30.6% vs. 15.1% and 20.1% vs. 12.8%, respectively). Conclusion Depression and anxiety are inadequately diagnosed and strongly associated with sex, severe headache, chronification and disability in patients with primary headache in China. To improve the health of patients with primary headaches, early screening for depression and anxiety is important.
Silent magnetic resonance angiography (S-MRA) is primarily utilized to assess the blood flow in aneurysms and parent vessels of treated intracranial aneurysms. This study aimed to compare the diagnostic value of S-MRA and three-dimensional time of flight (3D-TOF) MRA for unruptured intracranial aneurysms. We included patients diagnosed with unruptured intracranial aneurysms using digital subtraction angiography (DSA) who subsequently underwent S-MRA and 3D-TOF MRA. Two independent neuroimaging and neurointerventional doctors evaluated the DSA images and measured aneurysm dimensions. Using DSA results as the gold standard, we determined the sensitivity and specificity of S-MRA and 3D-TOF MRA, as well as their accuracy in measuring aneurysm size and identifying aneurysms with daughter sacs. We detected a total of 41 intracranial aneurysms (in 37 patients) on both S-MRA and 3D-TOF MRA, with both techniques achieving a sensitivity and specificity of 100%. For aneurysm height, the intraclass correlation coefficient (ICC) was 0.977 (P < 0.001) between S-MRA and DSA, and 0.908 (P < 0.001) between 3D-TOF MRA and DSA. For neck width, the ICC was 0.663 (P < 0.001) between S-MRA and DSA, and 0.563 (P < 0.001) between 3D-TOF MRA and DSA. In terms of daughter aneurysm detection, 3D-TOF MRA Sensitivity 40%; specificity 92%: positive predictive value 100%; S-MRA sensitivity 60%; specificity 89%; positive predictive value 42%. In conclusion, S-MRA and 3D-TOF MRA did not significantly differ in aneurysm detection ability. For the detection of aneurysm with dauthger sacs indicators, the sensitivity is also higher.
Background Exosomes derived from pre-stimulated mesenchymal stem cells (MSCs) have improved therapeutic effects in disease-associated microenvironments. In this study, we investigated the therapeutic potential of exosomes from MSCs stimulated with plasma from patients with liver failure (LF-Exos). Methods Untreated exosomes (NC-Exos) and LF-Exos were extracted and characterized by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), western blotting, and miRNA sequencing. We then examined the protective effects of LF-Exos on hepatocytes acutely injured by D-galactosamine (D-GalN)/lipopolysaccharide (LPS) co-treatment and on a mouse model of acute liver failure (ALF). Apoptosis was assessed using the CCK-8 assay and flow cytometry. Liver tissue damage was examined by hematoxylin and eosin staining and immunohistochemistry. The levels of signaling pathway proteins were determined by western blotting. Results Stimulation with plasma from patients with liver failure significantly altered the morphology of MSCs and reduced their proliferative activity. Gene chip analysis identified 31 differentially expressed miRNAs, and further analysis showed that these differentially expressed miRNAs may affect the PI3K-AKT signaling pathway. Compared to NC-Exos, LF-Exos induced AKT phosphorylation in hepatocytes and liver tissues, inhibited D-GalN/LPS-induced apoptosis in hepatocytes, and reduced pathological liver injury in the mouse model of ALF. Conclusion The biological effects of Exos were improved after stimulation with plasma from patients with liver failure. LF-Exos may inhibit the activity of the NLRP3 inflammasome and activate the PI3K-AKT signaling pathway to exert protective effects on acutely injured hepatocytes and a mouse model of ALF.
The mitochondrial calcium uniporter (MCU) complex mediates Ca²⁺ entry into mitochondria, which plays a crucial role in regulating cellular energy metabolism and apoptosis. Dysregulation of MCU is implicated in various diseases, such as neurodegenerative disorders, cardiac diseases, and cancer. Despite its importance, developing specific and clinically viable MCU inhibitors is challenging. Here, Berberine, a well‐established drug with a documented safety profile, is identified as a potent MCU inhibitor through a virtual screening of an FDA‐approved drug library. Berberine localizes within mitochondria and directly binds to the juxtamembrane loop domain of MCU. This binding disrupts the interaction of MCU with its essential regulator, EMRE, thereby inhibiting rapid Ca²⁺ entry into the mitochondria. Notably, Berberine pretreatment reduces mitochondrial Ca²⁺ overload and mitigates ischemia/reperfusion‐induced myocardial injury in mice. These findings establish Berberine as a potent MCU inhibitor, offering a safe therapeutic strategy for diseases associated with dysregulated mitochondrial calcium homeostasis.
Background Fatigue seriously reduces the sensory sensitivity and operational efficiency ofmariners, which is a major safety threat to ship navigation. This study aimed to investigate the relationship between resilience and fatigue and to explore the chain-mediated role of coping style and psychological stress among mariners during ocean voyages. Methods Using Connor-Davidson resilience scale, simplified coping style questionnaire, psychological stress self-evaluation test and multidimensional fatigue inventory, a questionnaire survey was conducted on 510 mariners from a Shandong fleet who finished a 32-day ocean voyage on September 12, 2023. SPSS 26.0 was employed to perform correlation analysis, while Amos 21.0 was applied to establish structural equation model and demonstrate the mediator effects. Results Daily working hours, negative coping style and psychological stress were positively correlated with fatigue, while resilience was negatively related to fatigue (all P < 0.01). The structural equation indicated that the 4 paths of “resilience → fatigue”, “resilience → psychological stress → fatigue”, “resilience → positive coping → psychological stress → fatigue” and “resilience → negative coping → psychological stress → fatigue” occupied 68.72%, 11.45%, 9.99% and 9.84% of the total effect, respectively (all P < 0.05), suggesting the validity of hypothesis. Conclusion Resilience exerts significant direct and indirect effects on fatigue of mariners during ocean voyages. In addition, coping style and psychological stress partially and serially mediate the relation between resilience and fatigue. The results have significant implications for the intervention and prevention of fatigue, providing additional evidence for the relationship between resilience and fatigue among mariners during ocean voyages.
Despite advances in diagnostic and therapeutic strategies, the prognosis of lung squamous cell carcinoma (LUSC) patients remains poor, and the potential of microbiome-based prognostic biomarkers and therapeutic targets remains largely unexplored. LUSC patient data from The Cancer Genome Atlas (TCGA), including microbial genus level abundance data and RNA sequencing (RNA-Seq) data, were used as a training dataset. Two other independent datasets GSE19188 and GSE157009 serve as validation datasets. A microbiome-based risk score (RS) model was constructed by univariate Cox regression analysis combined with the least absolute contraction and selection operator (LASSO) regression. 18 microbial genera were found to be significantly associated with RFS in LUSC patients. The microbial signature built with these microbial genera, exhibited robust predictive accuracy in both the training and validation datasets. Furthermore, hub mRNA between high- and low-risk groups were selected by XGBOOST and intersect with mRNAs screened by univariate Cox regression analysis, finally identifying four mRNA significantly associated with LUSC prognosis. This study reveals a complex interplay between the lung microbiome and genetic biomarkers, and identifies specific microbial-based and mRNA associated with prognosis in LUSC. These findings provide a basis for future studies aimed to elucidate the mechanisms underlying these associations and provide potential biomarkers for guiding treatment decisions and improving patient outcomes.
Background Sedentary lifestyles among adolescents have been associated with various health concerns, particularly regarding psychological well-being and sleep quality. However, the associative relationship between sedentary behavior and these health outcomes remains unclear. This study aims to clarify the association between sedentary lifestyle and psychological and sleep health among adolescents through propensity scores matching analysis. Methods A total of 2,846 adolescents from three universities participated in the study. Data on demographics, exercise habits, eating patterns, sedentary behavior, psychological health, sleep health, self-esteem, and social support were collected. A sedentary lifestyle was defined as sitting for more than six hours daily. Psychological health was assessed using the Generalized Anxiety Disorder-7 (GAD-7) scale for anxiety and the Patient Health Questionnaire-9 (PHQ-9) for depression, while sleep quality was measured using the Pittsburgh Sleep Quality Index (PSQI). Self-esteem was evaluated with the Self-Esteem Scale (SES), and social support was measured using the Social Support Rating Scale (SSRS). Propensity scores matching analysis was employed to investigate the associative relationship between sedentary lifestyles and the measured outcomes. Results Prior to propensity scores matching, significant differences were observed in baseline characteristics between participants with and without sedentary lifestyles, including gender (P=0.01), dietary habits (P<0.001), mobile device usage (P<0.001), stress events (P=0.001), physical activity (P<0.001), and chronic diseases (P=0.024). Participants with sedentary lifestyles exhibited higher scores on the GAD-7 (P<0.001), PHQ-9 (P<0.001), and PSQI (P<0.001), along with lower self-esteem (SES, P=0.041) and social support (SSRS, P<0.001) compared to their more active counterparts. Following propensity scores matching, no significant differences in baseline characteristics were found between the two groups (All P>0.282), indicating a successful matching process. Post-matching analysis revealed that individuals with sedentary lifestyles had significantly higher GAD-7 (P=0.002), PHQ-9 (P=0.013), and PSQI scores (P=0.001) than those without sedentary lifestyles, while no significant differences were found in SES (P=0.755) and SSRS (P=0.676). Conclusion Our findings indicate that a sedentary lifestyle is associated with poorer psychological health and sleep quality among adolescents, even after controlling for various demographic and lifestyle factors. These results underscore the importance of promoting physical activity and reducing sedentary behavior in this population to enhance their overall well-being.
Background Despite the promising clinical outcomes of artificial polyethylene terephthalate (PET) ligaments in isolated anterior cruciate ligament reconstruction (ACLR), their biomechanical performance after combined anterolateral ligament reconstruction (ALLR)/ACLR in anterolateral structure (ALS)/anterior cruciate ligament (ACL)–deficient knees has not been investigated. Purpose/Hypothesis The purpose of this study was to compare biomechanical performance in cadaveric knees between combined artificial ALLR/ACLR and isolated artificial ACLR using PET ligaments. It was hypothesized that combined artificial ALLR/ACLR would restore native knee stability and outperform isolated artificial ACLR in ALS/ACL-deficient knees. Study Design Controlled laboratory study. Methods Eight fresh-frozen cadaveric knees were tested using a robotic manipulator. Each knee was tested in 4 states: (1) ALS/ACL intact, (2) ALS/ACL deficient, (3) ACLR, and (4) ALLR/ACLR. The anterior tibial translation (ATT) and tibial internal rotation (IR) in each knee condition were measured under 3 loads: (1) 89 N of anterior tibial loading, (2) 5 N·m of IR torque, and (3) simulated pivot shift (combined 5 N·m of IR torque and 7 N·m of valgus load). Results During 89 N of anterior tibial loading, there were no significant differences in ATT between the isolated ACLR and ALLR/ACLR knees. During 5 N·m of IR torque, the mean tibial IR at 45° of flexion was significantly higher in the ACLR knees (32.49°± 7.96°) than in the ALLR/ACLR knees (21.78°± 3.03°) ( P < .05). During the simulated pivot shift, the mean ATT and tibial IR at 30° and 45° of flexion were significantly higher in the ACLR knees (ATT: 5.09 ± 2.74 mm at 30°, 5.43 ± 2.79 mm at 45°; IR: 30.08°± 7.31° at 30°, 32.55°± 6.48° at 45°) than in the ALLR/ACLR knees (ATT: 1.93 ± 2.71 mm at 30°, 1.17 ± 2.26 mm at 45°; IR: 22.12°± 4.05° at 30°, 22.18°± 3.37° at 45°) ( P < .05). Conclusion Combined artificial ALLR/ACLR restored native knee stability across multiple flexion angles and outperformed isolated artificial ACLR in ALS/ACL-deficient knees, particularly with respect to ATT and tibial IR during the pivot-shift test. Clinical Relevance The indications of the artificial PET ligament may be expanded to include combined ALLR/ACLR to restore knee stability better than isolated artificial ACLR in ALS/ACL-deficient knees.
Background Cupriavidus gilardii is a species of the genus Cupriavidus. Knowledge about the pathogenic characteristics of Cupriavidus gilardii is limited, especially cardiac infection with this bacterium has not been reported. Case presentation We encountered a case of pulmonary infection after myocardial infarction and heart failure. The initial empirical treatment with meropenem was ineffective. After cultured Cupriavidus gilardii, cefoperazone sulbactam and minocycline were used, and the infection and heart failure was improved for few days. Unfortunately, the patient eventually died of heart failure exacerbated by infection with Staphylococcus Epidermidis. Conclusion Meropenem has a limited therapeutic effect on this bacterium. Monitoring heart function and selecting effective antibiotics are crucial for improving the prognosis of patients suffered from heart disease and Cupriavidus gilardii infection.
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472 members
Haiyi Wang
  • Department of Radiology
Ruozhuo Liu
  • Neurology
Zhiqi Mao
  • Department of Neurosurgery
Guogang Xu
  • Health Management Institute
Yuan Yongyi
  • otolaryngology
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