Avans Hogeschool
  • Breda, Noord Brabant, Netherlands
Recent publications
Aim The objective of this study was to characterize new psychoactive stimulants’ effects on the inhibition of the monoamine transporters for dopamine (DAT), serotonine (SER) and norepinephrine (NET). Introduction Still, new psychoactive substances (NPS) are continuously emerging in Europe and globally. A large group of NPS is stimulants, drugs including cathinones, phenylethylamines, MDMA-analogues and derivatives of cocaine. Many stimulants affect the reuptake and storage of neurotransmitters in neurons. A major site of stimulant action is inhibition of the reuptake transporters DAT, SERT and NET. These proteins transport monoamine neurotransmittors across the nerve synaptic membrane, thereby ceasing the synaptic activity. Inhibition of the reuptake of the neurotransmitter will prolong the synaptic activity. It has been shown that the relative potency in inhibiting the DAT, SERT and NET transporters correlate with the observed drug effects and also their abuse potential. Especially the DAT/SERT inhibition ratio gives an indication of the abuse potential. Method The DAT/NET/SERT inhibition potency for a set of NPS stimulant drugs were determined by a rapid and automated method to measure inhibition of neurotransmitter transport, using a fluorescent dye mix. Transporter inhibition studies were conducted using cell lines expressing either the human DAT (in CHO-K1 cells), SERT (in HEK293 cells) or NET (in MDCK cells) transporters. The signal from the dye transported into the cells was measured using a TECAN Spark plate reader. Inhibition was measured at 15 concentrations in triplicate and repeated in three independent experiments. In total eighteen cathinones, six phenylethylamines and four arylcyclohexylamines were characterized. Results DAT, NET and SERT transport inhibiting curves were generated and IC50 values were determined for all substances. For DAT the potency varied between an IC50 of 1.03 nM for the cathinone MDPiHP to > 50,000 nM for the phenytlethylamine TMA-6. For SERT inhibition the potency was in general lower. The phenylethylamines were the extremes with a range from 79.6 nM for methamnetamine to > 36,000 nM for TMA-6. The DAT/SERT inhibition ratios for the tested substances varied from 0.4 to 3080. Four substances exhibited an MDMA-like ratio below 1. Two substances had an inhibition ratio varying between > 1 and < 10, close to cocaine at 3.37. Nineteen of the tested substances had a ratio between > 10 and 330. The highest inhibition ratios were identified for MDPiHP (705) and 3F-α-PiHP (3080). For the cathinone N-butyl Hexedrone, it was not possible to determine the ratio. With the exception of TH-PVP all other cathinones had a higher DAT/SERT inhibition ratio than cocaine and ten even more than amphetamine, indicating a high abuse potential. The substance with the highest potency for NET transport inhibition was MDPiHP at IC50 27 nM, which was the only one with higher potency than amphetamine. Three cathinones, MDPT (tBuoNE), N-butyl Hexedrone and TH-PVP had IC50 value for NET > 11,000 nM. For most of the characterized substances, the potency preference was DAT > NET > SERT, but there are exceptions [deschloroketamine, 3F-PCP, TMA-6, methamnetamine, 5-MMPA, TH-PVP, eutylone, N-ethyl heptylone, MDPT (tBuONE) and 3,4-methylenedioxy PV8]. With the large variation among the stimulants in potency, it is therefore, essential to characterize DAT, SERT and NET inhibition. Conclusion These results show that many of the emerging NPS stimulants are potent inhibitors of the monoamine transporters, although some lack activity or have a partial inhibitory effect.
Waste activated sludge (WAS) originating from kraft pulp mill effluent treatment plants represents an important environmental challenge for this industry. Anaerobic digestion is a promising option for WAS treatment, with the added benefit of biogas production. This paper presents the application of thermal, thermal-alkaline, and mechanical pretreatment methods in order to promote the solubilization of organic matter to enhance the anaerobic digestion of the WAS from a Brazilian Eucalyptus bleached kraft pulp mill. A total of 16 pretreatment operating conditions were compared. Chemical analyses showed an improvement in organic matter solubilization, with an increase greater than 7-fold for soluble chemical oxygen demand and 4-fold for biochemical oxygen demand. Nutrient solubilization showed an increase greater than 10-fold for total Kjeldahl nitrogen and 3-fold for total phosphorus. Theoretical biochemical methane potential was improved from 211 mLCH4/gVS for raw sludge to 333-343 mLCH4/gVS after mechanical pretreatment, 314-360 mLCH4/gVS after thermal pretreatment, and 373-378 mLCH4/gVS after thermal-alkaline pretreatment. In general, thermal-alkaline pretreatment showed the best results for all the evaluated parameters, with the advantage of requiring lower temperature and retention time when compared to thermal conditions.
Background Loss-of-function mutations of the multiple endocrine neoplasia type 1 ( MEN1 ) gene are causal to the MEN1 tumor syndrome, but they are also commonly found in sporadic pancreatic neuroendocrine tumors and other types of cancers. The MEN1 gene product, menin, is involved in transcriptional and chromatin regulation, most prominently as an integral component of KMT2A/MLL1 and KMT2B/MLL2 containing COMPASS-like histone H3K4 methyltransferase complexes. In a mutually exclusive fashion, menin also interacts with the JunD subunit of the AP-1 and ATF/CREB transcription factors. Results Here, we applied and in silico screening approach for 253 disease-related MEN1 missense mutations in order to select a set of nine menin mutations in surface-exposed residues. The protein interactomes of these mutants were assessed by quantitative mass spectrometry, which indicated that seven of the nine mutants disrupt interactions with both MLL1/MLL2 and JunD complexes. Interestingly, we identified three missense mutations, R52G, E255K and E359K, which predominantly reduce the MLL1 and MLL2 interactions when compared with JunD. This observation was supported by a pronounced loss of binding of the R52G, E255K and E359K mutant proteins at unique MLL1 genomic binding sites with less effect on unique JunD sites. Conclusions Our results underline the effects of MEN1 gene mutations in both familial and sporadic tumors of endocrine origin on the interactions of menin with the MLL1 and MLL2 histone H3K4 methyltransferase complexes and with JunD-containing transcription factors. Menin binding pocket mutants R52G, E255K and E359K have differential effects on MLL1/MLL2 and JunD interactions, which translate into differential genomic binding patterns. Our findings encourage future studies addressing the pathophysiological relevance of the separate MLL1/MLL2- and JunD-dependent functions of menin mutants in MEN1 disease model systems.
Diutina catenulata is an ascomycetous yeast, that is regularly fluconazole-resistant and increasingly reported as the cause of invasive infection in humans. Here, we describe the de novo genome assembly of the clinical D. catenulata type-strain CBS565 and provide insights into the genome and compared it to an Illumina-sequenced environmental strain.
Schools can enable students’ capability for lifelong physical activity (PA) by providing a physical, social and pedagogical context fostering students’ agency. This entails allowing them to develop their autonomy and ability to shape PAs according to what they value. This paper explores whether, and, if so, how, school practices provide the conditions for developing and employing PA-related student agency. We followed a multiple case study design, partnering with six secondary schools in the Netherlands. We based our qualitative content analysis on the schools’ policy documents and transcripts of interviews and focus groups with school management, teaching staff and supporting sports professionals. First, we analysed the space provided for PA-related student agency using the whole-of-school framework. Next, we used a social practice theory perspective to understand how each school practice allows for student agency. Results suggest that schools offer sufficient and varied PA opportunities but have not embedded deliberation with students on their meaningfulness and transfer to other contexts in their practices. We conclude that for schools to promote lifelong PA for students, there is a need for a pedagogical approach involving students’ perspectives, participation and reflections, enabling them to transfer PAs to other (future) life settings.
Measurement properties of the Working Alliance Inventory (WAI) and its various translations and adaptations for specific target groups have been investigated for over 30 years. No systematic review analyzing studies on measurement properties of the WAI has been conducted to date. COnsensus-based Standards for the selection of health Measurements INstruments (COSMIN) were developed for conducting high-quality systematic reviews on measurement properties in a transparent and standardized way. Aim of this study was to systematically review studies on measurement properties of the WAI, and its adapted versions, within psychotherapy, and other healthcare contexts using COSMIN criteria. PsycINFO, Medline, and EMBASE were searched (1989–2021). In all phases of the review procedure, study selection, data extraction, risk of bias assessment, rating of the quality of measurement properties, and rating of the quality of evidence for measurement properties, disagreement between reviewers was resolved by discussion. Results on validity, internal structure, reliability, construct validity, and responsiveness were analyzed. In total 66 studies were included. In most studies, evidence for measurement properties was according to COSMIN criteria, insufficient, lacking, or conflicting. Content validity was rated insufficient because neither patients nor healthcare professionals were involved in the development and validation process. Hence evidence for content validity of the WAI is unknown. Conflicting evidence was found for structural validity. Evidence for internal consistency could not be established. Limited evidence was found for inter-rater reliability and convergent validity. Conflicting evidence was also found for test-retest reliability and divergent validity. COSMIN criteria exposed persistent problems in validation studies of the WAI. These findings may indicate that measurement properties of the WAI are not up to current standards, or that COSMIN criteria may be less appropriate for assessing measurement properties of the WAI, or it could indicate both. The results of this systematic review suggest that WAI outcomes should be interpreted with caution and further research is needed regarding the content validity and hypotheses development. For the future, the theoretical framework underlying the measurement of the working alliance needs to be studied in psychotherapy and other health contexts, and tested in methodologically sound studies. Systematic Review Registration https://www.crd.york.ac.uk/PROSPERO/ , identifier CRD42019051428.
Ammonia recovery from anaerobic digesters via side-stream stripping is a technique to recover nitrogen from manure wastes. This study demonstrated a novel approach to determining ammonia recovery to maintain total ammonia concentrations in the digester in the range of 1.7-2.1 gN/L. Increasing the pH during stripping from 8, 8.5 to 9.5 did not affect the stability of the digester. Methane yields of 60-80 mL/(gVS.d) and volatile fatty acid concentrations of 0-500 mg/L were reported throughout its operation. The low solubilisation increase upon recirculation of the digestate explained the lack of change in methane yields due to side-stream stripping. Increasing the pH during stripping also did not affect the digester's operating pH, which was attributed to the neutralising effect of biogas as stripping gas. Therefore, total ammonia concentrations in the digester can be controlled by determining the extent of ammonia recovery, and the pH during stripping can be increased without compromising the digester’s stability.
This study investigated the selective production of volatile fatty acids (VFAs) during anaerobic mixed‐culture fermentation. The experiment used chicken manure (CM) as a potential substrate to produce high added‐value propionic acid and butyric acid under an alkaline environment. The conversion of CM into selective VFAs depends highly on operational conditions such as pH and redox balance. Therefore, the current experiment is designed to employ amino acid addition and develop a redox balance control method to control the final VFA profile. This study showed that 0.2-5.0% valine and threonine addition successfully enhanced propionic acid and butyric acid production during alkaline fermentation and hence decreased the proportion of acetic acid from 83% to approximately 47%. The oxidation-reduction potential (ORP) and redox cofactor ratio (NADH/NAD⁺) were measured to support the selective VFA production mechanism. The results obtained in this study bring extra value to the valorization of CM within the circular economy concept for selective value-added VFA production.
Background: Nurses and nursing students experienced an emotional burden while working during the COVID-19 outbreak. During the COVID-19 outbreak three questions for nurses working under these extreme circumstances were formulated: 1. What today's events do you remember? 2. How do you feel (physically and mentally)? 3. Do you have enough support? The purpose of this study was to obtain insight into whether nurses and nursing students perceive that the use of the three-questions-method contributes to effective coping with the emotional burden during the COVID-19 outbreak. Methods: Focus group interviews were held with hospital nurses (n = 11) and nursing students with internships in mental health care (n = 2), hospital (n = 9), and homecare/nursing home care (n = 3) in September 2020 followed by twenty semi-structured interviews one year later. Results: Almost all nurses and nursing students named factors that contributed to the emotional burden: fear, powerlessness, frustration, lack of knowledge about COVID-19, and pressure to pass the internship. Participants indicated that using the three-questions-method can help to effectively cope with the emotional burden during and after the COVID-19 outbreak. Conclusions: Using the three-questions-method offers added value in coping with emotional burden and can be used in education as well as in practice.
Presenting novices with examples and problems is an effective and efficient way to acquire new problem-solving skills. Nowadays, examples and problems are increasingly presented in computer-based learning environments, in which learners often have to self-regulate their learning (i.e., choose what type of task to work on and when). Yet, it is questionable how novices self-regulate their learning from examples and problems, and to what extent their choices match with effective principles from instructional design research. In this study, 147 higher education students had to learn how to solve problems on the trapezoidal rule. During self-regulated learning, they were free to select six tasks from a database of 45 tasks that varied in task format (video examples, worked examples, practice problems), complexity level (level 1, 2, 3), and cover story. Almost all students started with (video) example study at the lowest complexity level. The number of examples selected gradually decreased and task complexity gradually increased during the learning phase. However, examples and lowest level tasks remained relatively popular throughout the entire learning phase. There was no relation between students' total score on how well their behavior matched with the instructional design principles and learning outcomes, mental effort, and motivational variables.
In order to investigate the effect of time pressure on the execution of falling and falling-rising pitch movements on phrase-final syllables, we ran a production experiment with 119 speakers distributed over five regional varieties of West-Germanic spoken in the Netherlands and Standard Dutch. They realized nuclear Falls and Fall-Rises on four IP-final monosyllabic target words in which the duration of the sonorant portion of the syllable rhyme was varied by combining a short and a long vowel with a sonorant and obstruent coda consonant. The different contours were elicited with the help of a ‘Statement’ dialogue and a ‘Rhetorical question’ dialogue, respectively. Phonetic adjustments fell into three categories, target undershoot, increased f0 slopes, and durational easing, the latter either by f0 target retraction or sonorant rhyme lengthening. Talkers produced monosyllabic Fall-Rises quite generally, although avoidance in favour of a plain rising contour was evident in one-fifth of the cases of the shortest sonorant rhyme in the more prestigious varieties. Broadly, southwestern Zeelandic Dutch and northeastern Low Saxon differed most noticeably from each other in their treatment of the valley of the Fall-Rise, which Zeelandic maximally undershot and Low Saxon faithfully preserved. Urban Hollandic and Standard Dutch were similar, while West Frisian shares some features with Hollandic and some with Low Saxon. Apart from these effects of geographic contiguity, the data argue for an interpretation of the various measures alleviating time pressure within the more general conception of speech as a compromise between the interests of the speaker and those of the hearer in the process of speech communication.
Background Assertive Community Treatment (ACT) is a well-defined service delivery model for the care and treatment of the most severely mentally ill in the community with American origins. The Dutch have adapted the model in order to accommodate a broader range of needs and allow more flexible implementation. Functional Assertive Community Treatment (FACT) provides the intensity of care needed to help participants sustain life in the community as well as continuity of care over time for many vulnerable client populations.
Study design A cross-sectional inter-examiner agreement and reliability study on fresh frozen cadaver shoulders. Background Musculoskeletal ultrasound (MSU) is frequently used by physical therapists and radiologists to improve specific diagnosis in rotator cuff related pathology. The evaluation of the rotator cable seems to be important as stabilizing structure when cuff tears occur. Objectives To evaluate the inter-examiner agreement and reliability of MSU of the shoulder to detect rotator cuff-pathology and the involvement of the rotator cable in comparison to “dissection”. Methods Physical therapists, a radiologist and an orthopedic surgeon (dissection) investigated 40 fresh frozen cadaver shoulders in order to detect shoulder pathology including rotator cable involvement. Examiners were blinded to each other's findings. Results We found a strong and significant agreement between the raters: PTs, the radiologist and the dissector in this cadaver study for all rotator cuff, the long head of the biceps pathologies and in detecting abnormalities of the rotator cable. The kappa value was substantial to (almost) perfect agreement for all diagnostic outcome categories. Conclusions This study shows that among a limited group of physical therapists, one radiologist and a dissector a strong level of agreement with kappa values from substantial to (almost) perfect in finding subacromial pathology.
Burgers in de Nederlandse samenleving mogen verwachten hun leven te kunnen leven in vrijheid en zich individueel te kunnen ontplooien tot unieke, autonome burgers. Zelfmanagement, eigen regie en een samenleving waarin men zelfredzaam is, zijn daarin belangrijke pijlers. Daaromheen zijn wetgeving, beleid en professioneel handelen gebouwd. Zoals besproken in de eerdere delen is de Wmo samen met de Wet verplichte ggz een stimulering tot lokale en wijkgerichte afstemming van zorg. Professionals, naasten en (ex-)cliënten zijn het steeds meer met elkaar eens dat begeleiding en zorg ontvangen in de eigen vertrouwde omgeving, ongeacht de problematiek, heel vanzelfsprekend zou moeten zijn; en dat daarbij, waar nodig en mogelijk, de omgeving wordt ingeschakeld. Deze gedachte, dat het bevorderen van zelfredzaamheid van burgers goed is, stelt professionals uit het sociaal domein, de zorg, de ggz en het onderwijs geregeld voor ingewikkelde dilemma’s. De praktijken zijn weerbarstig en complex. Enerzijds heb je oog voor het individu, anderzijds gaat het om de gehele wijk en moet je rekening houden met de systemen in de samenleving als geheel. De focus op eigen regie, zelfmanagement en zelfredzaamheid geldt niet voor iedereen. En de behoefte aan of mogelijkheid tot het inschakelen en versterken van het sociale netwerk is ook niet voor iedereen dezelfde. Er is niet één systeem dat past op ieder individu, één mal waar iedereen zomaar in past. Met deze onderlinge behoefteverschillen zullen professionals én systemen dus rekening moeten houden.
Om goede zorg aan mensen met ernstige psychiatrische aandoeningen te kunnen bieden is integraal, geïntegreerd en interdisciplinair werken noodzakelijk conform het kluwenmodel. Interdisciplinair werken vraagt van professionals een intensieve en praktische samenwerking vanuit de verschillende perspectieven van alle disciplines. Toch kent de praktijk enkele generieke uitgangspunten die door professionals gedeeld worden, zoals het streven naar kwaliteit van leven en algemeen welbevinden, het honoreren, bewaken en bevorderen van de rechten van de mens (met een beperking), beseffen deel uit te maken van een groter proces van dienstverlening en verantwoordelijkheid voelen voor zowel het eigen aandeel als voor het slagen van het interdisciplinaire proces als geheel. Met de nodige creativiteit en unieke uitgangspunten van iedere professional wordt in interdisciplinaire samenwerking een generiek proces ingezet van betrekken, begrijpen, handelen, evalueren en onttrekken. Methodisch en procesmatig wordt in de triade en op basis van gezamenlijke besluitvorming een gezamenlijk plan van aanpak opgesteld en telkens geëvalueerd. Zo kan methodisch en in samenspraak de zorg worden afgeschaald, worden gestaakt of warm worden overgedragen.
De zorg voor mensen met mentale en psychosociale (gezondheids)problemen is al eeuwen in ontwikkeling. De ggz heeft zich ontwikkeld van gesloten institutie voor vele gemarginaliseerde groepen tot een cluster van organisatievormen voor mensen met allerhande mentale gezondheidsproblemen. Deze transformatie van de ggz kent parallellen met de transitie in het sociaal domein. Gemeenten maken de beweging naar de burger, de samenleving en de wijk om zelfredzaamheid en eigen regie van eenieder te bevorderen. De ontwikkelingen in de ggz en het sociale domein duren voort. Beide lijken zich meer en meer te verenigen in een ecologisch netwerk voor mentale gezondheid, waarin de burger centraal staat en samenwerking de weg is.
Integrale dienstverlening, waarin een breed aanbod aan diensten naadloos en drempelloos wordt aangeboden aan mensen met complexe psychiatrische problematiek, is het antwoord op de complexe netwerken in de wijk. Er lijkt overeenstemming bereikt over de uitgangspunten voor goede integrale zorg, echter, de uitvoering is in iedere wijk of regio, door grote lokale verschillen, zeer divers. Ontwikkelingsmodellen zoals het DMIC en RMIC helpen om integrale zorg actiegericht in het netwerk te implementeren. Zo kan de mate en wijze van integrale zorg optimaal aansluiten bij de doelgroep, van integrale samenwerking in een transfer- of dienstenmodel tot het punt dat er volledig integrale, geïntegreerde, zorg geboden wordt in een kluwenmodel aan mensen met een zorgindicatie Ernstige Psychiatrische Aandoeningen.
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Jos F Brouwers
  • Research Group Analysis Techniques in the Life Sciences
Michael Echteld
  • End of Life Care
Kees W Rodenburg
  • Academy of Health and Environmental Technology
Daniel Telgen
  • Center of Applied Research for Technical Innovation
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Hogeschoollaan 1, 4818 CR, Breda, Noord Brabant, Netherlands
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www.avans.nl
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+31885257500
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