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Immunohistochemical analysis. Dentin matrix protein-1 (DMP-1) and dentin sialoprotein (DSP) localization in newly formed tissue. Staining of respective positive technique controls for (a) DMP-1 (mammary gland) and (g) DSP (placenta) and negative technique control in the tooth (b and h). The positive control in the tooth for DMP-1 (c) and DSP (i) shows a layer of columnar odontoblasts surrounding the inner surface of dentin and positive staining for both odontogenic proteins as observed for the revascularized tooth (d and j). The intense staining of DMP-1 and DSP on the dentin mineralization front in the newly formed tissue (f and l) is similar to the controls (e and k). Bar corresponds to 100 µm

Immunohistochemical analysis. Dentin matrix protein-1 (DMP-1) and dentin sialoprotein (DSP) localization in newly formed tissue. Staining of respective positive technique controls for (a) DMP-1 (mammary gland) and (g) DSP (placenta) and negative technique control in the tooth (b and h). The positive control in the tooth for DMP-1 (c) and DSP (i) shows a layer of columnar odontoblasts surrounding the inner surface of dentin and positive staining for both odontogenic proteins as observed for the revascularized tooth (d and j). The intense staining of DMP-1 and DSP on the dentin mineralization front in the newly formed tissue (f and l) is similar to the controls (e and k). Bar corresponds to 100 µm

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... intense staining of the dentin matrix indicated the apparent formation of calcification nodes and positive staining on the dentin mineralization front [ Figure 4h]. The internal surface of the surrounding dentin showed a dot pattern with intense staining for DMP-1 and DSP, small integrin-binding ligand N-linked glycoprotein proteins that function as nucleators of extracellular matrix mineralization [ Figure 5f and l]. ...