mRNA expression of all 171 CAZymes during growth on pectins A–C, hemicelluloses D–E, glucans F–H, and raw corn stover I relative to expression on glucose.
Expression was quantified as log2(RPKM) with significantly differentially expressed genes on a given polysaccharide shown as triangles and unchanged genes as circles. The 56 purified CAZymes are red and others are blue.

mRNA expression of all 171 CAZymes during growth on pectins A–C, hemicelluloses D–E, glucans F–H, and raw corn stover I relative to expression on glucose. Expression was quantified as log2(RPKM) with significantly differentially expressed genes on a given polysaccharide shown as triangles and unchanged genes as circles. The 56 purified CAZymes are red and others are blue.

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Microbial metabolism of plant polysaccharides is an important part of environmental carbon cycling, human nutrition, and industrial processes based on cellulosic bioconversion. Here we demonstrate a broadly applicable method to analyze how microbes catabolize plant polysaccharides that integrates carbohydrate-active enzyme (CAZyme) assays, RNA sequ...

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... Thousands of enzymes are required to break down chemically diverse dietary components, including variable linkages found within complex polysaccharides. 32 Encoding the enzymes required to digest these compounds would require the host to dedicate an impractical fraction of its genome to this process. By making the large intestine a near-perfect environment for microbial fermentation, evolution arrived at a clever solution to the digestive challenge posed by the chemical complexity in food. ...
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