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Illustration of three sequence types of the cyrJ gene. (A) Schematic structures of cyrJ sequence types. White bar, cyrJ sequence; gray bar, repeat sequences; triangle, nucleotide deletions; C. raciborskii AWT205, reference strain. (B) Partial alignment of representative cyrJ gene sequences and deduced protein sequences . Repeat sequences were italicized. Dashed line, gaps introduced into the alignment; arrow, beginning of the repeat sequences.  

Illustration of three sequence types of the cyrJ gene. (A) Schematic structures of cyrJ sequence types. White bar, cyrJ sequence; gray bar, repeat sequences; triangle, nucleotide deletions; C. raciborskii AWT205, reference strain. (B) Partial alignment of representative cyrJ gene sequences and deduced protein sequences . Repeat sequences were italicized. Dashed line, gaps introduced into the alignment; arrow, beginning of the repeat sequences.  

Contexts in source publication

Context 1
... CyrI of C. raciborskii CHAB358 was found to be truncated because of an intragenic stop codon caused by a base transition from cytosine to thymine at bp 529 (according to C. raciborskii AWT205 numbering, used here and below). Single-base muta- tions were also observed within cyrI sequences from TG and SY reservoirs (see Fig. S3 in the supplemental material). Six sequences had similar mutations to the cyrI gene of C. raciborskii CHAB358. Base transversions from guanine to thymine at bp 304 of two sequences were observed and also formed stop codons. In addi- tion, four types of cyrI sequences were recognized according to intragenic sequence insertions compared ...
Context 2
... stop codons within the gene sequences and resulted in truncated protein sequences (see Fig. S4 in the supplemental material). A 48-nucleotide fragment was found to be repeated within the cyrJ sequences. Three cyrJ sequence types-Jtype1, Jtype2, and Jtype3-were identified based on copy numbers 1, 2, and 3 of this sequence repeat, respectively (Fig. 3). Jtype2 contained two sub- types, namely, Jtype2a with two intact repeats and Jtype2b with a 6-nucleotide deletion in the first repeat. Most cyrJ genes from CYN-producing strains belong to Jtype2a, and those of three C. raciborskii strains (i.e., AWT205, CS-505, and cyDB-1) and R. mediterranea FSS-150 belong to a third subtype Jtype2c ...
Context 3
... with two intact repeats and Jtype2b with a 6-nucleotide deletion in the first repeat. Most cyrJ genes from CYN-producing strains belong to Jtype2a, and those of three C. raciborskii strains (i.e., AWT205, CS-505, and cyDB-1) and R. mediterranea FSS-150 belong to a third subtype Jtype2c with a different 6-nucleotide deletion in the second repeat (Fig. 3). As displayed in Fig. S5 in the supplemental material, the sequence repeats within the cyrJ genes of the C/R group and Aphanizomenon sp. 10E6 were conserved. The second repeats in these species were divided into two groups based on nucleotide variations. One group contained the C/R group from Australia and Brazil, and the other ...