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i . Nervilia mackinnonii (Duthie) Schltr. A. habit; B. plant bearing flower; C, flower (spread out); D. labellum (front view); E. labellum (side view); F. column and ovary; G. anther; H. pollinia. ii . Nervilia plicata (Andrews) Schltr. A. habit; B. plant bearing flower; C, flower (spread out); D. labellum; E. anther. 

i . Nervilia mackinnonii (Duthie) Schltr. A. habit; B. plant bearing flower; C, flower (spread out); D. labellum (front view); E. labellum (side view); F. column and ovary; G. anther; H. pollinia. ii . Nervilia plicata (Andrews) Schltr. A. habit; B. plant bearing flower; C, flower (spread out); D. labellum; E. anther. 

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Article
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Nervilia makinnoni Duthie and Nervilia plicata (Andrews) Schltr. (Orchidaceae) are reported as new records to Nepal. Detail description, illustrations and relevant notes are provided. Key words: conservation status; herbarium; orchids; phenology.DOI: 10.3126/botor.v6i0.2920 Botanica Orientalis - Journal of Plant Science (2009) 6: 109-110

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Citations

... The flowers comprise 5 oblanceolate tepals and a labellum that varies in outline, colour and the degree to which its raised lateral margins envelop the column. Morphs with a white and yellow-marked labellum are known from India (Pagag et al. 2014), Nepal (Raskoti & Ale 2010), China (Song et al. 2007), Thailand (Gale & Watthana 2014), Vietnam (Averyanov 2011) and Borneo (Hamilton 2013), whereas morphs with a predominantly pink or purple labellum are reported from India (Hooker 1890), Bhutan (Pearce & Cribb 2002), China (Chen & Gale speciation in certain Bulbophyllum species (Teixeira et al. 2004). Indeed, micromorphological and histochemical characterisation of the labellar osmophore in two Ophrys species proved instrumental in refining hypotheses regarding highly specialised, deceptive attraction of pollinating male hymenoptera (Ascensão et al. 2005). ...
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Nervilia plicata is a morphologically variable terrestrial orchid with a wide geographic range in tropical Asia. Several forms have previously been recognised as distinct taxa due primarily to differences in the size, outline and colour of perianth parts. As a first step towards understanding the links between floral polymorphism, pollination ecology and genetic differentiation within the N. plicata complex, we sought to ascertain whether distinct colour morphs in 18 populations in Thailand and South China were correlated with discrete differentiation in macro- or micromorphological characters. Perianth morphology was studied in the field and under stereomicroscopy, and labellum anatomy was examined using light microscopy and scanning electron microscopy. Phenetic analyses comprising Principal Coordinate Analysis (PCoA) and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) of 16 characters revealed four clusters representing distinct floral morphs — pink morph with small, entire labellum; purple morph with large, undulate labellum; white and yellow morph with large, entire labellum; and white and yellow morph with small, entire labellum — among which the size, shape, density and periclinal wall surface of dome-shaped papillae in the secretory zone of the labellar adaxial epidermis varied discontinuously. Our results provide evidence of micromorphological differentiation among N. plicata populations in parts of tropical Asia. Pollinator observations and analyses of genetic diversity are required to establish whether this variation is of any ecological, evolutionary or taxonomic significance.
... At several instances, distributions are ambiguously written and they were cross-checked with actual herbarium specimens and field visits for proper authentication. We collected information from various published books, theses and web pages (Don 1825, Benerji and Thapa 1976, Malla et al. 1976, Benerji 1978, Benerji and Pradhan 1984, Polunin and Stainton 1984, Stainton 1988, Press et al. 2000, Rajbhandari et al. 2000, White and Sharma 2000, Anonymous 2001, Rajbhandari and Bhattarai 2001, Pearce and Cribb 2002, de Millevile and Shrestha 2004, Rajbhandari 2004, Rajbhandari and Dahal 2004, Ghimire 2009, Wu and Hong 2009, Maity et al. 2009, Raskoti 2009, Rajbhandari and Baral 2010, Web 1 and 2) and research papers that reported new species from Nepal (Panigrahi and Wood 1974, Tuyama 1975, Bajracharya et al. 1993, Shakya and Bania 1998, Shakya and Chaudary 1999, Shakya 2000, Tsukaya and Ohba 2000, Bajracharya 2001, Bajracharya et al. 2002, Shakya et al. 2002, Bajracharya and Shrestha 2003, Rajbhandari 2003, Bajracharya and Shrestha 2004, 2006, Panthi and Chaudhary 2005, Rai et al. 2007, Shakya and Shrestha 2007, Margonska and Kowalkowska 2008, Raskoti et al. 2008, Raskoti 2009, 2013, Raskoti and Ale 2009a, 2009b, 2011, Raskoti and Shakya 2009, Subedi et al. 2011, Web 1 and 2). Moreover, different collection trips were carried out in various parts of Nepal between 2001 and 2010. ...
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An annotated checklist comprised of 458 taxa of orchids known from Nepal is provided, including 104 genera, 437 species, 16 varieties, 3 subspecies and 2 forma and 18 endemic species. In Nepal, orchid species are distributed from 60–5200 m a.s.l. In the checklist, notes on altitudinal ranges, habit, habitat, global distribution, phenology, etc. are presented.
... At several instances, distributions are ambiguously written and they were cross-checked with actual herbarium specimens and field visits for proper authentication. We collected information from various published books, theses and web pages (Don 1825, Benerji and Thapa 1976, Malla et al. 1976, Benerji 1978, Benerji and Pradhan 1984, Polunin and Stainton 1984, Stainton 1988, Press et al. 2000, Rajbhandari et al. 2000, White and Sharma 2000, Anonymous 2001, Rajbhandari and Bhattarai 2001, Pearce and Cribb 2002, de Millevile and Shrestha 2004, Rajbhandari 2004, Rajbhandari and Dahal 2004, Ghimire 2009, Wu and Hong 2009, Maity et al. 2009, Raskoti 2009, Rajbhandari and Baral 2010, Web 1 and 2) and research papers that reported new species from Nepal (Panigrahi and Wood 1974, Tuyama 1975, Bajracharya et al. 1993, Shakya and Bania 1998, Shakya and Chaudary 1999, Shakya 2000, Tsukaya and Ohba 2000, Bajracharya 2001, Bajracharya et al. 2002, Shakya et al. 2002, Bajracharya and Shrestha 2003, Rajbhandari 2003, Bajracharya and Shrestha 2004, 2006, Panthi and Chaudhary 2005, Rai et al. 2007, Shakya and Shrestha 2007, Margonska and Kowalkowska 2008, Raskoti et al. 2008, Raskoti 2009, 2013, Raskoti and Ale 2009a, 2009b, 2011, Raskoti and Shakya 2009, Subedi et al. 2011, Web 1 and 2). Moreover, different collection trips were carried out in various parts of Nepal between 2001 and 2010. ...
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An annotated checklist comprised of 458 taxa of orchids known from Nepal is provided, including 104 genera, 437 species, 16 varieties, 3 subspecies and 2 forma and 18 endemic species. In Nepal, orchid species are distributed from 60–5200 m a.s.l. In the checklist, notes on altitudinal ranges, habit, habitat, global distribution, phenology, etc. are presented.
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