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Cepharanthine, a regulator of keap1-Nrf2, inhibits gastric cancer growth through oxidative stress and energy metabolism pathway
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  • May 2023
Cephalanthine (CEP), a bioactive compound derived from Stephania Cephalantha Hayata , is cytotoxic to various malignancies. However, the underlying mechanism of gastric cancer is unknown. CEP inhibited the cellular activity of gastric cancer AGS and HGC27 cell lines in this study. CEP induced apoptosis, reduced Bcl-2 expression, and increased cleav...
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... CEP inhibits the growth and proliferation of gastric cancer cells, and each group was repeated four times. The global gene expression prole demonstrates that CEP regulates gene translation in AGS cells. Compared to the control group, the CEP group produced 3685 differentially expressed genes (DEGs) with 1523 upregulated and 2162 down-regulated ( Fig. 4A and 4B). The number of up-regulated genes is lower than that of down-regulated genes, indicating that CEP signicantly inhibits gene transcription in AGS cells, resulting in cell function ...
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... examined these DEGs using the GO assay. This section focuses on the biological process of DEGs. GO analysis revealed that DEGs were primarily concentrated in the negative regulation of cell processes, cell communication regulation, and signal transduction regulation (Fig. 4C). We then conducted KEGG analysis. DEGs were signicantly enriched in DNA replication, the cell cycle, and other pathways (Fig. 4D). We only examined the mitochondrial membrane potential to determine if the mitochondria are affected to determine whether oxidative stress is involved in cell CEP toxicity. The JC-1 assay was used to ...
Context 3
... focuses on the biological process of DEGs. GO analysis revealed that DEGs were primarily concentrated in the negative regulation of cell processes, cell communication regulation, and signal transduction regulation (Fig. 4C). We then conducted KEGG analysis. DEGs were signicantly enriched in DNA replication, the cell cycle, and other pathways (Fig. 4D). We only examined the mitochondrial membrane potential to determine if the mitochondria are affected to determine whether oxidative stress is involved in cell CEP toxicity. The JC-1 assay was used to determine the effects of CEP on AGS and HC27 cells. The increase in CEP concentration increased the number of JC-1 monomers (green ...