Source publication
Preprint
Full-text available
Objective:Here, we explored the expression of SYNM by means of Gene Expression Omnibus (GEO) and investigated its prognostic significance as well as potential functions in gastric cancer (GC). Methods:Toward this goal, differential gene expression analysis, univariate Cox regression, Lasso regression, best subset regression, Gene Set Enrichment Ana...

Contexts in source publication

Context 1
... of patients with high expression of SYNM mRNA was signicantly lower than that of patients with low expression (P < 0.001, (Fig. 1F, G). In addition, we also analyzed the relationship between SYNM mRNA expression and different clinical characteristics and the results showed that SYNM mRNA expression was higher in females than in males (P < 0.05, Fig. 2H); The mRNA expression of SYNM in intestinal GC was higher than that in diffuse GC (P < 0.01, Fig. 2H); SYNM mRNA expression increased with T, N and Stage staging (P < 0.001, P < 0.05, P < 0.001, Fig. ...
Context 2
... expression (P < 0.001, (Fig. 1F, G). In addition, we also analyzed the relationship between SYNM mRNA expression and different clinical characteristics and the results showed that SYNM mRNA expression was higher in females than in males (P < 0.05, Fig. 2H); The mRNA expression of SYNM in intestinal GC was higher than that in diffuse GC (P < 0.01, Fig. 2H); SYNM mRNA expression increased with T, N and Stage staging (P < 0.001, P < 0.05, P < 0.001, Fig. ...
Context 3
... expression and different clinical characteristics and the results showed that SYNM mRNA expression was higher in females than in males (P < 0.05, Fig. 2H); The mRNA expression of SYNM in intestinal GC was higher than that in diffuse GC (P < 0.01, Fig. 2H); SYNM mRNA expression increased with T, N and Stage staging (P < 0.001, P < 0.05, P < 0.001, Fig. ...
Context 4
... order to verify the results of bioinformatics analysis, the mRNA expression of SYNM in GC tissues and normal tissues was veried by qRT-PCR. SYNM mRNA expression in normal tissues was signicantly higher than that in GC tissues (P < 0.01, Fig. 2E). Then, Western blotting was performed to quantitatively detect protein expression level. SYNM expression level in normal tissues was signicantly higher than that in GC tissues (P < 0.01, Fig. 2D). Finally, the protein expression level of SYNM was detected qualitatively and locationally by immunohistochemistry. According to the ...
Context 5
... in GC tissues and normal tissues was veried by qRT-PCR. SYNM mRNA expression in normal tissues was signicantly higher than that in GC tissues (P < 0.01, Fig. 2E). Then, Western blotting was performed to quantitatively detect protein expression level. SYNM expression level in normal tissues was signicantly higher than that in GC tissues (P < 0.01, Fig. 2D). Finally, the protein expression level of SYNM was detected qualitatively and locationally by immunohistochemistry. According to the specication of SYNM antibody, SYNM was positively expressed in human myocardium tissue ( Fig. 2A), proving the effectiveness of SYNM antibody. Then we detected SYNM protein expression in 120 pairs of ...
Context 6
... expression level. SYNM expression level in normal tissues was signicantly higher than that in GC tissues (P < 0.01, Fig. 2D). Finally, the protein expression level of SYNM was detected qualitatively and locationally by immunohistochemistry. According to the specication of SYNM antibody, SYNM was positively expressed in human myocardium tissue ( Fig. 2A), proving the effectiveness of SYNM antibody. Then we detected SYNM protein expression in 120 pairs of cancer tissues and normal tissues of GC patients. As shown in Fig. 2B and C, we observed diffuse cytoplasmic staining in normal tissues, while most cytoplasmic and nuclear staining were not observed in GC tissues, and light cytoplasmic ...
Context 7
... was detected qualitatively and locationally by immunohistochemistry. According to the specication of SYNM antibody, SYNM was positively expressed in human myocardium tissue ( Fig. 2A), proving the effectiveness of SYNM antibody. Then we detected SYNM protein expression in 120 pairs of cancer tissues and normal tissues of GC patients. As shown in Fig. 2B and C, we observed diffuse cytoplasmic staining in normal tissues, while most cytoplasmic and nuclear staining were not observed in GC tissues, and light cytoplasmic staining was found in a few tumor tissues. As shown in Table 2, the positive rate of SYNM expression in GC was 47.5% (57/120) and the negative rate was 52.5% (63/120). The ...

Similar publications

Article
Full-text available
Background: Nonspecific Orbital Inflammation (NSOI) remains a perplexing enigma among proliferative inflammatory disorders. Its etiology is idiopathic, characterized by distinctive and polymorphous lymphoid infiltration within the orbital region. Preliminary investigations suggest that PALMD localizes within the cytosol, potentially playing a cruci...
Article
Full-text available
Introduction: The 5-year survival of gastric cancer (GC) patients with advanced stage remains poor. Some evidence has indicated that tryptophan metabolism may induce cancer progression through immunosuppressive responses and promote the malignancy of cancer cells. The role of tryptophan and its metabolism should be explored for an in-depth understa...