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Wastewater surveillance (WWS) has received interest from researchers, scientists, and public health units for its application in monitoring active COVID-19 cases and detecting outbreaks. While WWS of SARS-CoV-2 has been widely applied worldwide, a knowledge gap exists concerning the effects of enhanced primary clarification, the application of coag...

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... (at 60 mg/L Fe 3+ ), which lie within the optimal operating pH range of 5.0-8.5 for ferric precipitation during enhanced primary clarication ( Crittenden et al., 2012). Measurements of N1 and N2 copies/g oscillating between 1500 and 4880 copies/g showed no statistically signicant change between the two pH conditions (p = 0.173 and p = 0.536) ( Fig. 4A and B, respectively). However, measurements of PMMoV copies/g showed a statistically signicant decrease in the samples from 9.0 x 10 6 ± 3.2 x 10 6 at pH 6.6 to 5.5 x 10 6 ± 1.7 x 10 6 at pH 7.6 (p < 0.05) (Fig. 4C). This nding runs contrary to the original hypothesis that reducing the sample pH would result in a higher PMMoV viral ...
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... between 1500 and 4880 copies/g showed no statistically signicant change between the two pH conditions (p = 0.173 and p = 0.536) ( Fig. 4A and B, respectively). However, measurements of PMMoV copies/g showed a statistically signicant decrease in the samples from 9.0 x 10 6 ± 3.2 x 10 6 at pH 6.6 to 5.5 x 10 6 ± 1.7 x 10 6 at pH 7.6 (p < 0.05) (Fig. 4C). This nding runs contrary to the original hypothesis that reducing the sample pH would result in a higher PMMoV viral signal in solids since viral particles reportedly bind better to wastewater colloids at lower pH due to a reduction in the negative charge of the virus ( Walshe et al., 2010). PMMoV viral particles are negatively ...
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... due to possible degradation or alteration in the integrity of the virus or wastewater ocs ( Yang et al., 2022). Despite these changes in the PMMoV RNA measurements, the PMMoV-normalized SARS-CoV-2 N1 and N2 gene regions associated with these measurements are shown to not be signicantly affected by this change in the measured PMMoV in the solids ( Fig. 4D and E). Therefore, the pH decrease associated with the Fe 3+ addition, isolated from the effects of Fe 3+ itself, shows no statistical change in the PMMoV-normalized SARS-CoV-2 measurements in the wastewater ...