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a Enlarged cell staining both for chromatin (dark central mass) and for biotin (pink material surrounding nuclei). Bar=50 microns b Enlarged cell and neighboring syncytium. Faint pink stains from biotin are seen in both structures. This polyploid single cell was found in a dish being induced for dome formation. Such cells are also found in cultures that are not being induced. Bar=50 microns
Source publication
The results in this paper demonstrate that Ishikawa endometrial monolayer cells become multinucleated by a process of nuclear “donation” from neighboring cells. As the resulting polyploid cell detaches from the colony in which it was formed, it is possible to detect mitonucleon(s) in the center of the cell. The mitonucleon is a transient mitochondr...
Contexts in source publication
Context 1
... structure in fig. 1a is larger than the surrounding cells and stains intensely for chromatin using hematoxylin and eosin. Cells immediately around it are oriented toward, sometimes even appearing to be physically in contact with, this enlarged central cell. That orientation together with the density of staining in the receiving cell suggests nuclei are ...
Context 2
... a chemical change in chromatin as they begin to fuse into aggregates. The neighboring multinucleated cell is far more compact, but still at least four to five times larger than monolayer cells. There is no visible border around the syncytium other than what is formed by neighboring mononucleated monolayer cells. The multinucleated cell in fig. 1a has a distinct cell membrane and some of the neighboring cells are fusiform with one polar end appearing to be in contact with the enlarged cell. Staining only for endogenous biotin which we and others have shown is bound to mitochondrial carboxylases (Fleming et.al., 1998;Gamache et. al., 2003 ), fig 2a reveals two additional ...
