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Fig 5 - DNA-binding miniproteins based on zinc fingers. Assessment of the interaction using nanopores

Fig. 5 (a) A single a-HL pore inserted in a lipid membrane allows the flow of ions in response to an applied voltage. The ionic current of the open pore is shown below. (b) A double stranded DNA containing the target CTCTC-A 2 T 2-GAGAG binding site inserted in a hairpin followed by a ssDNA overhang (see the ESI † for the full sequence). This DNA is driven to the pore by the electric field. The threading, unzipping and translocation of the DNA causes the characteristic signal shown below. (c) When the trivalent peptide is bound to the DNA there is an additional step with the DNA-protein complex on top of the pore, which causes a new high-conductance level (c1). Once the protein is detached, the reaction proceeds as with free DNA (c2-3). Below is the ionic current signal when a protein-DNA complex is analyzed. 
(a) A single a-HL pore inserted in a lipid membrane allows the flow of ions in response to an applied voltage. The ionic current of the open pore is shown below. (b) A double stranded DNA containing the target CTCTC-A 2 T 2-GAGAG binding site inserted in a hairpin followed by a ssDNA overhang (see the ESI † for the full sequence). This DNA is driven to the pore by the electric field. The threading, unzipping and translocation of the DNA causes the characteristic signal shown below. (c) When the trivalent peptide is bound to the DNA there is an additional step with the DNA-protein complex on top of the pore, which causes a new high-conductance level (c1). Once the protein is detached, the reaction proceeds as with free DNA (c2-3). Below is the ionic current signal when a protein-DNA complex is analyzed. 
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